[A method of studying the exocrine function of the livers of dogs with intact gallbladders and intact hepato-intestinal circulation].
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Biomedical subjects
Publications and source records attributed to M F Nesterin.
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In test conducted on rats kept for 60 days on a complete standard ration that included one of the fat varieties (sunflower, cotton oils, butter, mutton fat, margarine) a hypocholesterolemic effect of the diet with sunflower oil was recorded, which was attended by an increased excretion of bile acids and cholesterol in the bile. With the rats fed on a low-protein or fat-rich rations that included sunflower oil (30 and 60 per cent, respectively) a marked change in the ratio between the bile acids and cholesterol, in favour of the latter, was noted.
In tests set up on rats the effect of methandrostenolone on the external secretion of the liver, fermentative activity of the pancreatic tissue and histological and hisochemical findings subsequent to an investigation of internal organs were studied. Introduction of methandrostenolone led to changes in bilification and to a reduced concentration basic components of the bile, to a distinctly pronounced rise in the level of the trypsin inhibitor and to a depressed activity of trypsin in the pancreatic tissue. It also resulted in a change of histochemical and fermentative indices in the tissue of the liver, spleen and other organs.
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Gain in body mass, mucosal morphology and microflora of the contents of the cecum were experimentally studied in adult growing male rats kept for 15 days on parenteral (intravenous) feeding with various nutrient mixtures. The rats on parenteral feeding and the control ones fed orally (via the natural route), kept on diets including all the major nutrients, gained in body mass about 40 g, during the experiment. Body mass of the animals on parenteral feeding with mixtures not containing the nitrous component was practically unchanged by the end of the experiment in all the groups of animals. Dystrophic changes in the cecum were detected in rats kept completely on parenteral feeding. These changes manifested mainly in altered nuclear structure of cryptal epithelium, being especially grave, up to the death of cells, in cases of reduced depth of the cryptae, in rats fed diets free from nitrous components. In rats parenterally fed diets including the principal nutrients the level of anaerobic Lactobacilli in the cecum was significantly reduced, as compared to rats on natural feeding.
In tests conducted on rats the process of eliminating the endogenous formed lipids with bile was studied and the discovered changes were contrasted against the lipids metabolism disorders in the liver and blood of the animals receiving rations with differing quantitative and qualitative content of carbohydrates. A balanced ration with saccharose led to a higher quantity and radioactivity of the total lipids and triglycerides in the liver and blood. In the bile a fall in the level of the cholic acid, cholesterol and the lipid complex and an increased radioactivity in the composition of the lipids complex, cholesterol and phospholipids were observed. A ration with an elevated level of starch did not upset the lipids metabolism in the liver and blood. In the bile the lipids complex decreased, the quantity of cholic acid increased, while the radioactivity in the lipids complex and phospholipids was rising. A carbohydrate-rich ration with saccharose raised the content of total lipids, triglycerides and of the ether-bound blood cholesterol and their radioactivity. The described diet resulted in an increased amount of the bile and in a drop therein of the level of the lipids complex, cholesterol and phospholipids and in an accrued radioactivity in them.
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In tests with rats observations over changes in the lipids metabolism in the liver, blood and bile with application of low-protein diets and different proportions of carbohydrates and fat were carried out. The No-1 ration containing 5 per cent of protein and an augmented to 70 per cent carbohydrates (starch) amount led to accumulation in the liver and to increased radioactivity in triglycerides and ether-bound cholesterol, to diminution of the amount and radioactivity of phospholipids in the liver and blood and to a lowered lipids complex concentration in the bile of cholic acid and proportions of glycoconjugates and tauroconjugates. The No-2 ration, where a greater carbohydrates quota was represented by a mixture of starch and saccharose, and also the No-3 ration with an elevated share of fat, produced similar, but to a higher degree, pronounced changes in the liver, blood and bile.
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