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Biomedical subjects

M Brozman

Publications and source records attributed to M Brozman.

At least 55 records · Page 3Linked to original sources
PubMed1984

Pericapillary spongy sheaths in the human spleen. An immunohistological study.

Antisera to extracts of human hyaline cartilage allow distinct visualization of extracellular structures of adventitial sheaths around arterial capillaries in the red pulp and marginal zone in the human spleen. These pericapillary sheaths have a prominently spongy character indicating their essential filter function. In childhood they are most marked. In premature and stillborn infants they sometimes occupy whole stretches of pulp cords, whereas in old age they are frequently diminished to small formations lacking a distinctly spongy structure.

Adolescent↗
PubMed1984

Morphology of haemorrhagic shock, systemic immunocomplex reaction and systemic anaphylactic reaction. A comparative study.

Diagnosis and pharmacological control of shock represent the problems of everyday public health practice. Our experimental approach using three different shock models contributes to further elucidation of the pathogenesis of haemorrhagic shock as well as shock developing during systemic anaphylactic reaction (SAR) and systemic immunocomplex reaction (SIR). It could help to explain why adequate diagnosis and application of mostly proved and commonly used therapeutic procedures may fail under some conditions. For the future, based on our and other experimental data obtained in particular by techniques of electron microscopy, it will be perhaps possible to consider some new interpretations of findings occurring in humans who died in shock reactions and in those with a suspicion of clinically diagnosed shock respectively.

Anaphylaxis↗
PubMed1984

[Kaposi's sarcoma (an electron microscope case study)].

Endothelial histogenesis of sarcoma Kaposi was referred to in a case of 36-year old man studied by electron microscopy. Characteristic features corresponding to the literature were found: Weibel-Palade bodies, a ferritin-like material, phagocytosed erythrocytes, nuclear bodies, microfilaments and microtubules. Myelinated and unmyelinated axons were considered as remnants of original nervous tissue destroyed by the tumour or as nerve fibres accompanying proliferated vascular structures.

Adult↗
PubMed1983

The use of anti-cartilage antisera in the study of human spleen.

Antisera against human hyaline cartilage, particularly antisera against collagenase-treated residue obtained after extraction of cartilage homogenate with 3.4 mol dm-3 NaCl and extraction with 0.05 mol dm-3 sodium citrate buffer at pH = 3.2, reacted with different fibrillary and membraneous structures of the human spleen. Very intensive immunofluorescence was observed in special spongiform structures at the end of the arterial system, referred to as spongy arterial terminals. Fibrillary and membraneous structures situated perivascularly, in lymphatic sheaths and the periphery of follicles displayed similar reactions. The discontinuous walls of sinuses and reticular fibrils in pulp cords reacted less intensively. The given antisera are used to advantage as immunohistologic reagents since they clearly visualize the basic structure, and particularly the spongy arterial terminals, otherwise inconspicuous. In the hyaline cartilage of the rib and knee, the cartilage channels yielded the most intensive reaction. Of the other organs studied the marked reaction in the mesangium of the renal glomerulus was the most remarkable.

Cartilage↗
PubMed1983

Immunofluorescence and enzyme cytochemical analysis of arterial terminals in the human spleen.

The simultaneous application of immunofluorescent and enzyme cytochemical methods made it possible to analyze the components of the walls of the arterial branches and arterial capillaries, and particularly the spongy arterial terminals in the red pulp of human spleen. The endothelium was identified by alkaline phosphatase demonstration, the basement membranes (BM) by means of antisera to glomerular BM, the media by using the same antisera, and the adventitia by means by antisera to hyaline cartilage, which are presumed to react particularly with adventitial structures. In the spongy arterial terminals visualized by means of anti-hyaline-cartilage antisera, the endothelium and mostly also BM could be detected, yet the enzyme cytochemical reaction to acid phosphatase and alpha-naphthyl acetate esterase and the immunofluorescent reaction to lysozyme yielded negative reactions. The findings suggest that neither macrophages nor other cells containing the given enzymes in detectable form are accumulated in these structures.

Alkaline Phosphatase↗