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Biomedical subjects

M Brozman

Publications and source records attributed to M Brozman.

At least 37 records · Page 2Linked to original sources
PubMed1987

Human hyaline cartilage--biochemical and immunochemical study.

Hyaline cartilage from the knee or rib of newborns was divided in neutral salts into a soluble and insoluble part. The insoluble part was treated with collagenase and was separated into 2 peaks by gel chromatography on Sephadex G-200. The 1st peak revealed a low concentration of proteins, no hydroxyproline, and very high levels or uronates. In the 2nd peak, very high values of hydroxyproline and very low values of uronate were found. Thus the 1st peak contained only proteoglycans and no collagen, while the entire collagen was found in the 2nd peak. The individual fractions were inoculated into rabbits to obtain antibodies. Compared to the fraction containing macromolecular proteoglycans, antisera proved remarkably suitable particularly for analysis of human spleen, yet also of other organs. These anti-cartilage antisera visualized primarily spongy structures of pericapillary sheaths and circumferential reticulum of the periarterial lymphatic sheaths, but to a lesser extent also other extracellular structures of the spleen and of other organs.

Antibodies↗
PubMed1986

[Micromorphometric study of changes in the gastric mucosa after proximal selective vagotomy in rats].

Micromorphometry produced a graphic hardware for computer in a study of gastric mucosal hyperemia in rats after proximal selective vagotomy. Hyperemia was found in intervals of 1 day and 7 days after operation in gastric body, whereas lasted only 1 day in pyloric antrum [after 7 days the picture was similar to the sham-operated animals]. Histology did not help to distinguish between a passive and active hyperemia.

Animals↗
PubMed1985

Outer mantle zone of the follicle in the human spleen.

The follicular circumferential connective fibre reticulum, which is a continuation of the circumferencial reticulum (CR) of the periarterial lymphocyte sheaths, was visualized at the periphery of lymphoid follicles in the human spleen by means of anticartilaginous antiserum. If this CR is considered to be the borderline between the follicular and extrafollicular structures, then at the inner aspect of the CR and at the periphery of the fully developed follicle two mantle zones can be distinguished: an inner zone consisting of small lymphocytes, and an outer consisting of medium-sized lymphocytes. Both all groups are B-lymphocytes displaying positive surface IgM. The reticulum is the same in both mantle zones and it is completely different from the reticulum of the marginal zone. In some instances it is so sparse as to appear to be absent completely. The newly delimitated outer mantle zone also has a specific cellular composition differing from the marginal zone. Most importantly, it does not possess that mixture of lymphocytes and other blood cells which is considered to be the defining feature of the marginal zone. The characteristic cellular components of the outer mantle zone are the densely accumulated medium-sized lymphocytes, with other cells interspersed very sporadically.

B-Lymphocytes↗
PubMed1985

The adult human female urethra. Enzyme-histochemical study.

In the female urethra, the activity and distribution of 15 enzymes was determined by using both conventional and special histochemical methods. The enzymatic equipment differed according to the type of epithelial lining whose variation is characteristic for the female urethra. In the stratified squamous epithelium of the urethra, alkaline phosphatase, beta-glucuronidase, acetyl-beta-D-glucosaminidase, thiamine pyrophosphatase, and glucose-6-phosphatase exhibited but minimal or no activity, yet the other 10 enzymes studied displayed activity particularly in basally situated cells. Nearer to the lumen of the urethra, the activity in the epithelium kept decreasing and was mostly absent in superficial and desquamated cells. In the pseudostratified columnar and in the transitional epithelium of the urethra, the majority of enzymes showed an evenly distributed activity at all epithelial levels. In the apical parts of the most superficially situated cells bounding the lumen of the urethra, a distinct narrow zone of higher activity was observed. It was seen not only on determining the majority of dehydrogenases but also on examining acid phosphatase and naphthyl esterase. The endocrine cells occurring in the uroepithelial lining of the female urethra displayed, yet always with the exception of squamous epithelium (Zaviacic et al. 1983), distinct activity of acid hydrolytic enzymes, and of the enzymes studied it was particularly acid phosphatase. The majority of the demonstrated enzymes, of the dehydrogenases priority, is to be given to succinate dehydrogenase, enabled to differentiate readily between the highly active striated muscle fibers located in the most peripheral parts of the excisions along the urethral circumference and the smooth musculature of the urethral wall with a lower or only minimal activity.

Acetylglucosaminidase↗
PubMed1985

Anatomical pathways from the white to the red pulp in the human spleen.

In the human spleen we failed to find marginal zone bridging channels which in rats and mice are said to serve as return routes for lymphocytes from the white into the red pulp. In human spleens, using anticartilaginous antisera which distinctly visualized extracellular structures, in some parts we found the periarterial lymphocyte sheath to be closely attached to the red pulp, so that lymphocytes and other material could pass from the white pulp directly into the red pulp and vice versa. The strips of reticular fibres that seemed to bridge the marginal zone between the follicles and the red pulp proved to be components of reticular structures around the arteries, passing from the periarterial lymphocyte sheath into the follicles or from the follicles through the marginal zone into the red pulp.

Adolescent↗
PubMed1985

Immunohistological study of the red pulp in the human spleen.

By means of anti-cartilage antisera, on using immunofluorescent technique, special spongy structures were detected in the red pulp of the human spleen, occurring around arterial capillaries and at their terminals. At some sites, these formations were closely attached to the walls of sinus, suggesting a direct connection between the arterial and venous system. Exceptionally they were found to be attached to the wall of the veins in the red pulp. In the majority of instances, anti-cartilage antisera distinctly visualized also fibrillar and membranous adventitial structures around small arterial branches, which were more or less connected with the spongy structures of pulp cords. In several-month-old infants the spongy structures occupied relatively large areas of pulp cords, and exceptionally also the entire segment of the pulp cord, whereas at advanced age, these structures were very small. Yet they invariably contained structures of sheathed capillaries. These spongy arterial terminals seem to represent specifically formed parts of pulp cords, constituting a particularly specialized part of the vascular system of the human spleen.

Adolescent↗