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Biomedical subjects

L Jakab

Publications and source records attributed to L Jakab.

At least 55 records · Page 3Linked to original sources
PubMed1992

[Effect of estrogen on the blast transformation of lymphocytes and interleukin-2 production in lupus erythematosus].

The mitogenic response of peripheral lymphocytes was investigated in 12 patients with systemic lupus erythematosus and in healthy female volunteers who were on 11 and without 9 contraceptive pills. The effect of estrogen (ethinyl-estradiol 10(-5)-10(-6)-10(-7)M) was studied on Phytohaemagglutinin and Pokeweed mitogen induced blastogenic transformation and interleukin-2 production of peripheral lymphocytes in vitro. We observed a significantly depressed Phytohaemagglutinin induced lymphoblastic transformation both in patients and women taking oral contraceptive in presence of 10(-5)M estrogen as compared to normal controls. However there was no significant alteration neither in the response of lymphocyte nor in the production of interleukin-2 using of Pokeweed mitogen. The stimulataneous inhibition of the interleukin-2 production proved to be moderate. Marked significant correlation (r greater than = 0.8) vas detected between lymphoblastic transformation and interleukin-2 production in healthy females. Correlation coefficient measured in females taking oral contraceptive (r less than = 0.64) and patients with systemic lupus erythematosus (r less than = 0.34) suggest that in these groups the inhibition of lymphoblastic transformation is due to the inhibition effect of estrogen on the interleukin-2 production.

Adolescent↗
PubMed1991

[The role of the central nervous system in lupus erythematosus].

Central nervous system involvement of systemic lupus erythematosus was observed in 34 (36%) of the 94 patients studied between 1970-1990. A review of the diagnostic methods and therapy for central nervous system lupus is presented. The diagnosis of primary cerebral lupus was based on the history, physical examination and on the results of the cerebrospinal fluid analysis, CT-scan and EEG. Intractable headache (22/34), behavioural abnormalities (18/34), cranial neuropathy (16/34) occurred most frequently among neuropsychiatric symptoms. Immunoglobulin analysis of the cerebrospinal fluid proved to be the most sensitive method for detecting clinical activity (in 20/23). Central nervous system involvement was suggested by conventional serological test to a lesser degree. Alterations on CT scan and EEG were found in 17/27 and 14/26 of cases, respectively. IgM, IgA, and IgG indexes (indicators of intratechal immunglobulin synthesis) decreased when the central nervous system events subsided after successful treatment but the CT abnormalities (e. g. atrophy) were not altered.

Electroencephalography↗
PubMed1991

Serum alpha 2-HS glycoprotein concentration in patients with hematological malignancies. A follow-up study.

We observed significantly reduced serum alpha 2-HS glycoprotein concentrations in patients with acute lymphocytic, acute nonlymphocytic, chronic granulocytic and chronic myelomonocytic leukemias, Hodgkin's and non-Hodgkin's lymphomas, myelofibrosis, and multiple myeloma, but not in patients with chronic lymphocytic leukemia and polycythemia vera, as compared with healthy controls. We followed the serum level of the protein for 18 months. Patients with infectious complications, those receiving cytostatic treatment, and those in the preterminal period had further reduced serum alpha 2-HS glycoprotein levels. The reduction of serum alpha 2-HS glycoprotein concentration was primarily due to decreased production caused by infiltration of the liver, a hepatotoxic effect of cytostatic treatment, and, to a lesser degree, to increased consumption. We found statistically significant negative correlations between serum alpha 2-HS glycoprotein concentration and erythrocyte sedimentation rate, serum aspartate aminotransferase and alkaline phosphatase activities, and IgG and IgM concentrations. The determination of the alpha 2-HS glycoprotein concentration is useful for the assessment and follow-up of the clinical status and therapy of patients with hematological malignancies and also has prognostic significance.

Adolescent↗
PubMed1991

The effect of the alpha 2-HS-glycoprotein on the mitogen-induced lymphoblastic transformation and IL-2 production.

Authors investigated the in vitro effect of alpha 2-HS-glycoprotein on the lymphoblastic transformation and interleukin-2 production. The observations were made on the peripheral blood lymphocytes of healthy persons, on those of women taking oral contraceptives and on those of patients with systemic lupus erythematosus. The alpha 2-HS-glycoprotein inhibited the mitogen responses both in physiologic and in the halved concentrations. The inhibition of the interleukin-2 production seemed less remarkable.

Adolescent↗
PubMed1990

Effect of mannozym treatment on plasma fibronectin concentration in germfree and conventional mice.

As a result of a single intraperitoneal treatment with 1 mg/ml of Mannozym (M) the plasma fibronectin (FN) level was significantly increased both in germfree (Gf) and conventional (Cv) mice, and though it started from a lower value in Gf mice, it rose to a similar level to that of Cv animals. The maximum of FN level was observed on the first day after treatment both in Gf and Cv mice. It had returned to normal by the 7th day in Cv mice, but it was higher in Gf mice as compared to untreated controls even on the 14th day. Thus the increase of FN level was of higher degree and longer duration following M treatment in Gf mice than in Cv animals. In treated Gf mice the plasma FN concentration was in the same range as in untreated Cv mice even at the termination of experiment. Both in Gf and Cv mice, there was a relative spleen weight increase, the degree of which was similar, but the duration was longer in Gf mice than in Cv ones.

Adjuvants, Immunologic↗
PubMed1990

Correlations between serum alpha 2-HS-glycoprotein concentration and conventional laboratory parameters in systemic lupus erythematosus.

Serum alpha 2HS-glycoprotein (A2HSG) concentrations of 63 patients with systemic lupus erythematosus (SLE) were determined, and found to be significantly low compared to those of 59 healthy blood donors. The diminution of serum A2HSG concentration was proportional to the degree of activity of SLE, and was not influenced by secondary infections. There was a statistically significant positive correlation between serum A2HSG and the C3 complement component levels. A negative correlation between serum A2HSG and IgG, IgA concentration and anti-DNA activity was observed. Serum A2HSG was significantly low in cases of positive for the following laboratory parameters: anti-nuclear antibodies, circulating immune complexes and LE cell phenomenon. We found no correlation between serum IgM concentration, cryoglobulins, latex agglutination and serum A2HSG levels. The unusually good negative correlation between A2HSG pathogenetical role of this glycoprotein in SLE. The determination of A2HSG concentration may be of clinical importance in SLE.

Adult↗
PubMed1989

[Systemic lupus erythematosus and pregnancy].

The authors report on the successful outcome of pregnancy of a young woman with systemic lupus erythematosus. Her disease presented with severe renal involvement. The delivery was followed by lasting, complete remission. Even at the time of the report both the mother and her child are in a good health. On the occasion of this case-history the authors attempts, to summarize the present knowledge of pregnancy in systemic lupus erythematosus.

Adult↗
PubMed1989

Binding of fibronectin to human lipoproteins.

High molecular mass adhesive glycoprotein plasma fibronectin binds to isolated HDL and LDL lipoprotein fractions in a solid phase radioimmunoassay. Mean dissociation constants of interaction of fibronectin and immobilized HDL and LDL lipoproteins isolated from eight patients with type IIa and type IV hyperlipoproteinemia are 7.8 +/- 3.2 X 10(-7) mol/l and 6.8 +/- 2.6 X 10(-7) mol/l, respectively. Fibronectin can also bind to HDL and LDL isolated from six healthy subjects with mean dissociation constants of 2.07 +/- 0.45 X 10(-6) mol/l and 2.25 +/- 0.48 X 10(-6) mol/l, respectively. The binding is not dependent on the presence of divalent cations. Fibronectin-lipoprotein interaction is inhibited by soluble lipoproteins. There is no observable interaction between fibronectin and VLDL fraction. Binding of fibronectin to HDL and LDL lipoproteins can have an in vivo significance, since the interaction may play a role in the metabolism, deposition of lipoproteins into the vessel wall and in atherogenesis.

Adult↗
PubMed1989

Plasma fibronectin concentration in germfree and conventional mice of various age.

Lower plasma fibronectin (FN) levels were detected in C3H germfree (Gf) mice as compared to conventional (Cv) ones. FN levels increased with aging both in Gf and Cv mice. Stimulation of the immune system with live Newcastle disease virus (NDV) vaccine increased the FN levels in young germfree mice, but had no effect in conventionals.

Aging↗
PubMed1989

Effect of selenium, vitamin E and riboflavin supplementation of the feed on the humoral and cell-mediated immune responses of growing pigs.

The feed of weaned piglets of Hungarian Large White X Duroc and Dutch Landrace X Duroc genotype was supplemented with 0.5 mg selenium, 50, 100 or 150 mg vitamin E, and 2.5 or 5 mg riboflavin per kg. Feed supplementation enhanced the cytotoxic reaction and elevated the antibody titres produced against purified horse gamma globulin antigen. However, as compared to the control the differences were not significant. Feed supplementation exerted a beneficial, though varying, influence on the indices of cell-mediated immunity. The proportion of rosette-forming cells and blastogenic transformation induced by specific (horse globulin) and nonspecific (phytohaemagglutinin, PHA) mitogens underwent the most expressed and most significant increase in pigs fed 5 mg selenium, 100 mg vitamin E and 5 mg riboflavin per kg of feed. On the other hand, feed supplementation failed to enhance the responsiveness to intradermal PHA (type IV allergic reaction).

Animal Feed↗
PubMed1986

[Determination of plasma fibronectin in myeloproliferative and lymphoproliferative diseases and other malignancies].

Plasma fibronectin concentration was determined by electroimmunodiffusion and laser nephelometry in 40 healthy persons and 321 patients with myelo- and lymphoproliferative diseases and other malignancies. Decreased fibronectin concentrations were found in patients with leukemia, Hodgkin's and non-Hodgkin's lymphomas, myelofibrosis, polycythaemia rubra vera and angioimmunoblastic lymphadenopathy. Elevated fibronectin level was detected in patients with multiple myeloma. In patients with cancer of the lung, stomach and colon, fibronectin level was found in the normal range. Decreased fibronectin concentration was observed in patients with cancer of the breast and prostate. Lower plasma fibronectin concentrations were detected in all groups of patients with infectious septical complications as compared to the patients without infections.

Adult↗
PubMed1985

Fibronectin on the surface of human lymphocytes.

Fibronectin was detected by immunofluorescence technique on the surface of one part of separated normal peripheral blood lymphocytes by using FITC-conjugated anti-human fibronectin antibodies. Approximately one-fifth of isolated B cells and 7% of O cells contained surface-bound fibronectin but T cells failed to stain. There were no detectable free receptors for fibronectin on the surface of lymphocytes of different subsets as it was studied with FITC-labelled purified fibronectin. The percent of B and O cells bearing surface bound fibronectin was markedly decreased in patients with acute and chronic lymphocytic leukemias.

Adolescent↗
PubMed1985

Binding of fibronectin to DNA: new application of the Crithidia luciliae immunofluorescence test.

Binding of purified plasma fibronection to Crithidia luciliae kinetoplast DNA was demonstrated by fluorescence microscopy. The method was suitable for the detection of fibronectin in human plasma diluted 1:256 and 1 microgram/ml concentration of isolated fibronectin. Purified human Clq, monoclonal human myeloma proteins of IgG1 and IgG3 subclasses and calf thymus DNA inhibited the binding of fibronectin to kinetoplasts. The method can be used as a functional assay for fibronectin and for various materials containing fibronectin.

Animals↗
PubMed1983

Effect of aminoethyl-isothiuronium (AET) treatment on intestinal cell proliferation during ageing.

To establish whether aminoethyl-isothiuronium X bromide X hydrobromide (AET) treatment influenced intestinal cell proliferation during ageing, juvenile and aged female rats were treated with AET for a period of 20 days. Subsequent incorporation of 3H-thymidine was studied in the epithelial cells of the duodenal and jejunal crypts. It was found that after AET administration the major changes occurred in the duodenum, i.e. the number of the villous epithelial columnar cells were found to be elevated during senescence with a markedly higher level of mitotic numbers in the crypts. Changes were less in the ageing jejunum, here neither the cells nor the number of mitoses were as altered as those of the duodenal region. In general, the labelling indices of crypt cells increased in young ages after AET treatment. The findings can be explained by the role of AET with respect to nuclear metabolism.

Aging↗