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Biomedical subjects

L G Miller

Publications and source records attributed to L G Miller.

At least 199 records · Page 11Linked to original sources
PubMed1967

Antibacterial sensitivity of Bifidobacterium (Lactobacillus bifidus).

The antibacterial sensitivity patterns of gram-positive, nonsporeforming, anaerobic bacilli variously classed as Lactobacillus bifidus, Actinomyces bifidus, or Bifidobacterium were studied by the plate dilution method. A total of 34 strains, mostly from human feces, was studied. Three species, B. longum, B. adolescentis, and B. bifidum, were represented with 11, 11, and 6 strains, respectively. The other six strains fell into four other species. Most strains of all types resisted 100 mug/ml or more of neomycin, polymyxin B, and nalidixic acid. They were somewhat less resistant to kanamycin and still less so to streptomycin. All strains were inhibited by less than 1 mug/ml of penicillin G and erythromycin, by 3.1 units or less per ml of bacitracin, by 3.1 mug/ml or less of chloramphenicol, and by 6.2 mug/ml or less of tetracycline and lincomycin. Most strains were inhibited by 3.1 mug/ml of vancomycin. Results were very variable with cephalothin and nitrofurantoin, with some strains quite resistant. With half of the drugs tested, there were moderate differences in sensitivity between different species. These data are discussed in relation to the effect of antimicrobial agents on bifid bacilli in the normal human fecal flora, in relation to the implications thereof, and in relation to the usefulness of several agents (particularly neomycin, nalidixic acid, and polymyxin B) in selective media for Bifidobacterium.

Anti-Bacterial Agents↗
PubMed1967

Antibiotic susceptibility patterns as aids in classification and characterization of gram-negative anaerobic bacilli.

Patterns of susceptibility of gram-negative anaerobic bacilli to antibiotics have been found to be distinctive and of significant help in classification and identification. Five major groups of gram-negative anaerobic bacilli have been defined on the basis of morphological and biochemical criteria. Antibiotic sensitivity patterns conform to these groupings and provide additional taxonomic criteria. The Bacteroides fragilis group is resistant to penicillin G, whereas the other groups are generally sensitive. B. fragilis strains are relatively sensitive to erythromycin, whereas the Sphaerophorus necrophorus group is resistant. B. melaninogenicus strains, B. oralis, and Fusobacterium are all more sensitive to kanamycin and neomycin than the other two groups. Kanamycin is more active against Fusobacterium strains than neomycin, but less active against all other groups. Colistin or polymyxin B is useful for distinguishing between the resistant B. fragilis and the sensitive S. necrophorus. Antibiotic susceptibility determinations may be more readily performed in clinical laboratories than certain biochemical tests recommended for differentiation of the gram-negative anaerobic bacilli and may serve as helpful adjuncts to morphological and biochemical observations in classifying and characterizing these organisms. The use of standardized procedures for antibiotic susceptibility tests is essential if comparable results are to be obtained in different laboratories.

Anti-Bacterial Agents↗
PubMedDate not supplied

Negative therapeutics.

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Cultural Characteristics↗
PubMedDate not supplied

A comparative evaluation of oral contraceptive use and associated compliance issues in a rural population.

One hundred thirty-three patients were enrolled in a study designed to evaluate and compare oral contraceptive use in three rural communities. The patients averaged 31 years old (range, 13 to 49 years); 92% were white, 4.5% Hispanic, and 3% Native American. Genora, Ortho-Novum, and Triphasil were the most frequently prescribed oral contraceptives. Triphasic oral contraceptives accounted for one third (n = 64) of 203 prescriptions, and conventional monophasic preparations accounted for two thirds. Twenty percent (n = 26) of the patients studied were non-compliant. The most frequent causes of noncompliance cited were cost and inconvenience. However, generic alternatives were requested in only 43% of the cases for which generic equivalents were available. Less-expensive alternatives (eg, generic products, medroxyprogesterone acetate injection) should be advocated to deter patient noncompliance because of cost issues. Drug interactions and adverse effects were not reported in our patient population and thus did not contribute to noncompliance.

Adolescent↗
PubMedDate not supplied

MIF-1 and Tyr-MIF-1 augment GABA-stimulated benzodiazepine receptor binding.

Behavioral evidence in laboratory animals and human beings indicates possible links between the endogenous opiate and gamma-aminobutyric acid (GABA)-benzodiazepine receptor systems, especially with regard to antagonistic properties. To assess possible interactions between endogenous opiate antagonists and benzodiazepine receptor binding, we evaluated the effects of the peptides MIF-1 and Tyr-MIF-1 on benzodiazepine receptor binding in mouse brain membranes. Neither peptide affected receptor binding in cortex over a broad dose range, but both peptides significantly augmented GABA-stimulated benzodiazepine receptor binding at GABA concentrations of 10(-8) and 10(-7) M. Rosenthal-Scatchard analysis indicated that the increase in binding was largely due to increased apparent affinity. Both peptides augmented GABA-enhanced binding at low doses (MIF-1 10(-11) M, Tyr-MIF-1 10(-13) M) with decreased effects at higher doses. In cerebellum and brainstem, MIF-1 tended to enhance GABA-stimulated binding but Tyr-MIF-1 was inactive. These results indicate benzodiazepine-opiate and benzodiazepine-peptide interactions.

Animals↗
PubMedDate not supplied

Use of patient education and monitoring software in community pharmacies.

OBJECTIVE: To determine the level of use of health education and monitoring software programs in independent community pharmacies in rural locales. DESIGN: The use of seven software programs that deliver patient education and provide blood pressure monitoring was assessed at five sites of the Nebraska Drug Information Network (NDIN) for one month. Direct Access, a Windows-based software program, documented which programs were accessed and for what time period. RESULTS: During a one-month period, 326 accesses were logged representing 44 hours of computer time. Extrapolated to all 30 sites of NDIN, this represents 264 hours and 1,956 accesses monthly. On average, each pharmacy logged 8.8 hours within 65 accesses each month. Each access averaged 8 minutes. DynaPulse (167 accesses; 16.4 hours) was the most frequently used product, followed by Home Medical Advisor Pro (54 accesses; 8.9 hours) and Mayo Clinic The Family Pharmacist (35 accesses; 8.3 hours). The least amount of time was spent with BodyWorks (0.6 hours) but Wellness Checkpoint had fewest accesses (7 accesses). CONCLUSION: Applied medical informatics is assuming a larger role in daily clinical practice as pharmacy practitioners strive to better inform their patients. This is congruent with the finding that a better informed public can be a healthier public. This study demonstrates that computer systems are readily used by patients and pharmacists in rural communities.

Adult↗