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Biomedical subjects

L Elsborg

Publications and source records attributed to L Elsborg.

At least 73 records · Page 4Linked to original sources
PubMed1980

[Trimethoprim].

Explore the source record for details and available documents.

Drug Resistance, Microbial↗
PubMed1980

Chromatographic isolation and characterization of folate binding proteins in porcine intestinal epithelial brush border membrane.

Gel filtration studies on solubilized porcine intestinal epithelial brush border membranes labelled with [3H] folate revealed three distinct protein peaks, Mr approximately 25 000, Mr approximately 80 000 and Mr greater than 130 000 (listed in order of decreasing folate binding affinity). The two large molecular size proteins may represent polymerized forms of the Mr approximately 25 000 peak. Folate binding proteins were eluted in front effluent after DEAE-Sepharose CL-6 B chromatography (pH 6.3, 30 mM NaCl) of a solubilized membrane preparation. This means that the cationic forms of these proteins predominate at pH 6.3. In conclusion, folate binding in brush border membranes display characteristics in many respects similar to those of high-affinity folate binding in other tissues and body fluids.

Animals↗
PubMed1979

Pernicious anaemia as a risk factor in gastric cancer.

In order to assess the risk of gastric cancer (GC) developing in patients with pernicious anaemia (PA), the prevalence of PA was analysed in all patients with GC notified to the Danish Cancer Registry in 1972. Among 877 patients with GC, PA had previously been diagnosed in 19 (2.2%). In seven of these, PA had been diagnosed only shortly before GC. Accordingly, the diagnosis of PA could be regarded as unquestionable only in the remaining 12 cases (1.3%). In either case, the frequency of PA was significantly higher than in a reference group of patients with cancer of the colon who had been selected in the same way. Calculation of the incidence of GC in PA patients showed that this was about three times higher than in the general population. The annual risk of GC was calculated to be 0.3%. In PA patients, the tumour was primarily localized to the body and fundus of the stomach, whereas it mainly involved the antral and pyloric region in patients without PA. In view of the low cancer rate it is concluded that routine gastroscopy and barium meal examination are not indicated in PA patients in general. Whenever a patient with PA complains of dyspepsia, examinations with gastroscopy and barium meal should, however, be carried out on liberal indications.

Adult↗
PubMed1979

Vitamin B12 concentrations in psychiatric patients.

In an attempt to evaluate the possible relationship between vitamin B12 deficiency and mental disease, the blood content of vitamin B12 was investigated in 835 consecutive psychiatric patients. Low serum vitamin B12 values were found in approximately 10% of these patients, due to latent pernicious anaemia in one case, post-gastrectomy in seven cases and small intestinal resection in one case. In the remaining 72 cases vitamin B12 deficiency was probably caused by nutritional insufficiency. After correction of the dietary defect there was a spontaneous increase in serum vitamin B12 in 75% of these patients. No specific psychiatric syndrome was connected with hypovitaminosis B12, but a preponderance of arteriosclerotic dementia suggests that low serum vitamin B12 values are secondary to mental illness leading to apathy and loss of appetite. Most cases will recover without further vitamin B12 supplements. But some patients may need treatment because of severe mental and physical disabilities.

Adolescent↗
PubMed1979

A new method for the determination of gastrointestinal transit times.

A new method for the determination of the gastrointestinal transit time (TT) is described. The normal range of the TT was 40--95 h in 58 normal subjects. No age or sex variation of the TT was disclosed. In 25 out-patients with the irritable bowel syndrome the TT was determined before and after 3 weeks' treatment with Vi-Siblin. Of these patients, 20% initially showed a prolonged TT; this decreased during treatment with Vi-Siblin. In the patients with normal TT, this remained unchanged during the treatment. Our study suggests that patients with long TT, long intervals between bowel movements, and frequent attacks of colicky abdominal pain may derive benefit from a plant fibre supplement to their diet. The method for the determination of the TT described is well suited to out-patient studies--also in large populations--since it is relatively easy to perform, stool collection is unnecessary, and the method shows good reproducibility.

Adult↗
PubMed1979

The role of liver passage for conversion of pteroylmonoglutamate and pteroyltriglutamate to active folate coenzyme.

In an attempt to clarify the possible site of conversion of folates to active methylated coenzyme forms mono- and triglutamate was injected rapidly into the portal vein of dogs. Samples of blood were removed at various intervals from catheters placed in the portal vein, the hepatic vein and the femoral artery for assay carried out both with L. casei, S. Faecalis and liquid scintillating counting. Unreduced monoglutamates were extracted in the liver, immediately converted to methyltetrahydrofolate and released again to plasma from which it was slowly taken up by peripheral tissue. Reduced folates were refused by the liver. Triglutamates were extracted exclusively in the systemic circulation, converted to unreduced monoglutamate later to be reduced in the liver. No tissue displacement of folate was demonstrated. Only small amounts of monoglutamates, mainly reduced forms, were lost in the urine during the experiments. The kinetics of unreduced monoglutamate suggest a one compartment system, while the kinetics of methyltetrahydrofolate and triglutamate may implicate a two compartment system.

Animals↗
PubMed1979

Folate deficiency in chronic inflammatory bowel diseases.

In a consecutive study of 216 outpatients with chronic inflammatory bowel diseases (CIBD) low serum and erythrocyte folate levels were found in 59% and 26%, respectively. In patients with low folate levels in both serum and erythrocytes, megaloblastic changes in the bone marrow ere found in 67% (29 out of 44). Their folate intakes were borderline. Absorption studies with tritiated folate showed low absorption values on repeated examinations in 23% of the patients with low folate values (9 out of 40), with no relationship to the intake of salazosulphapyridine. In patients with low folate values the reticulocyte count was elevated (related to the dose of salazosulphapyridine), and the 51Cr erythrocyte survival was decreased. It is suggested that folate deficiency in CIBD is of multiple origin: inadequate diet, malabsorption, and chronic drug-induced low-grade haemolysis. The clinical consequence of the findings remains to be evaluated.

Bone Marrow↗
PubMed1979

Folate intake by teenage girls and by pregnant women.

In a dietary analysis comprising 110 girls aged 13-15 years and 87 pregnant women the mean folate intake proved to be less than one-third of the Recommended Dietary Allowances. Among the girls there was a significantly positive correlation between folate intake and energy intake, while this did not apply to the pregnant women. The reason was that the excess energy intake during pregnancy was only modest, and in many cases it was met by energy calories of low nutritional value. If supplements of folic acid are needed during pregnancy, it is recommended to start prophylaxis as early as possible, as folate intakes by potential mothers (teenage girls) are insufficient to secure a high depot level for folates before pregnancy.

Adolescent↗
PubMed1979

Iron intake by teenage girls and by pregnant women.

A dietary study among 110 teenage girls and 87 pregnant women revealed that their mean iron intake was lower than the Recommended Dietary Allowances. The explanation is assumed to be a relatively low energy intake. Considering the composition of Scandinavian food, in which about 40% of the energy calories are derived from animal products, it is suggested that about 90% of all pregnant women can cover their iron requirement without further supplements.

Adolescent↗
PubMed1978

Pharmacokinetics of sulfadiazine and trimethoprim in man.

Sulfadiazine (SDZ) 800 mg and trimethoprim (TMP) 160 mg were given orally to 10 normal subjects and the concentration of SDZ and TMP in serum and urine was followed for 24 h. Both drugs showed a significant negative correlation between individual "peak" concentrations in serum and the body weight of the subject. Twelve hours after dosing the serum concentration was 12 to 25 microgram/ml for SDZ and 0.3 to 1.1 microgram/ml for TMP. Individual concentration ratios between SDZ and TMP in serum were 4.8 (1 h)--145 (24 h), and in the urine the ratio was close to 6 throughout the 24 h collection period. The range of urinary concentrations was from 65 to 400 microgram/ml for SDZ and from 13.8 to 93.4 microgram/ml for TMP. The fraction (formula: see text) was 21% during the 0--8 h period, 33% during the 8--15 h period and 41% during the 15--24 period. The average "t1/2" was 15.2 +/- 7.4 h for SDZ and 7.4 +/- 1.9 h for TMP. Individual subjects showed a significant correlation between the serum clearance of TMP and SDZ (p less than 0.01) and also between the renal clearance of the two drugs (p less than 0.05). The serum clearance was significantly correlated with the renal clearance for TMP but not for SDZ. For SDZ Vd was significantly negatively correlated with the elimination constant; for TMP no such correlation was found. The serum clearance of SDZ was significantly correlated with the percentage of SDZ which was excreted as the (presumably) acetylated compound. The renal clearance of SDZ was independent of the serum concentration of SDZ. There was a highly significant negative correlation between the renal clearance and serum concentration of TMP, as well as for "acetylated SDZ". The renal clearance of "acetylated SDZ" averaged more than six times that of unconjugated SDZ. With increased urine flow the renal clearances of TMP and SDZ were significantly increased.

Absorption↗