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Biomedical subjects

L Ekelund

Publications and source records attributed to L Ekelund.

At least 37 records · Page 2Linked to original sources

Knee motion in total knee arthroplasty. A roentgen stereophotogrammetric analysis of the kinematics of the Tricon-M knee prosthesis.

The three-dimensional kinematics of the Tricon-M knee prosthesis during active knee flexion and extension were recorded in 11 patients with arthrosis or rheumatoid arthritis using roentgen stereophotogrammetric analysis. Twenty-three normal knees constituted the control group. The prosthetic knees displayed the same degrees of freedom regarding rotational and translational movements as the normal knees, although the kinematics were different. A combination of internal rotation, abduction, and lateral translation of the tibia was recorded during flexion, and the reversed movements were recorded during extension. During the first 25 degrees of flexion, these movements were small, reflecting the high congruency between the articular surfaces, while beyond 25 degrees they increased. The normal knees displayed a combination of internal rotation, adduction, and medial translation of the tibia during flexion and the reversed movements during extension. The prosthetic knees also exhibited an increased posterior displacement during increasing flexion when compared with the normal knees. There was a correlation between the positioning of the femoral component in the sagittal plane and the recorded anterior/posterior translations. In conclusion, the kinematics of the Tricon-M knee prosthesis significantly differ from the normal knee, probably because of the design of the prosthesis and the absence of the cruciate ligaments.

Aged↗

Low field (0.02 T) magnetic resonance imaging of bone and soft tissue tumors.

Extremely low field magnetic resonance imaging (MRI) was evaluated in 16 patients with bone and soft tissue tumors, both benign and malignant. Twelve of the lesions were located in the lower extremity, 2 in the gluteal and 2 in the shoulder region. The findings were compared to the results of computed tomography (CT). Low signal-to-noise ratio and poor spatial resolution result in an image quality which is inferior to that obtained with MR imaging at higher field strengths. In spite of this, the diagnostic information seems to be at least comparable to that obtained from CT.

Adolescent↗

Tumour radioimmunolocalization in nude mice by use of antiplacental alkaline phosphatase monoclonal antibodies.

Three monoclonal antibodies and their Fab and Fab'2 fragments with specificity against human placental alkaline phosphatase (PLAP) were evaluated for tumour immunolocalization of human PLAP-producing Hela Hep 2 tumours in nude mice. The antibodies and their fragments were labelled with 125I and injected intraperitoneally in mice with developing Hep 2 tumours. The animals were followed individually for 14 days with repetitive computerized gamma-camera recordings, which enable quantitation of several crucial parameters, i.e. the time-dependent antibody uptake in the tumours, decrease in background activity and tumour/background ratio. Excellent radioimmunolocalization was obtained with both the intact PLAP-specific immunoglobulins and their fragments but not with the nonspecific antibodies. No background subtraction had to be used. As much as 15% of the initially injected dose could be visualized in the tumours and for the uncleaved mab up to 80% of the radioactivity in the animals was retained in the tumours after 14 days, a considerably longer observation time than usually reported in such tumour xenograft models. The Fab and Fab'2 fragments were found to be excreted fast with less than 5% of the injected dose remaining in the animals after 48 h, but still with positive specific localization to the tumours after an initial high uptake in the kidneys. The results are encouraging and indicate significant potentials of the PLAP-antiPLAP mab system for immunolocalization studies in patients.

Adenocarcinoma↗

Pharmacoangiography of the penile arteries.

Angiography may play an important role in the evaluation of patients with erectile failure and in whom abnormal Doppler studies indicate arterial insuffiency. The angiographic examination should give information about major pelvic arteries as well as the peripheral branches of the internal pudendal artery. We describe a method for performing pharmacoangiography of the penile arteries, which involves the local injection of a vasodilator into the corpus cavernosum. Angiography during pharmacologic erection provides optimal visualization of the penile arteries and helps to differentiate functional from organic vascular disorders.

Angiography↗

Venous invasion in renal cell carcinoma: a correlative clinical and radiologic study.

Angiography, cavography, ultrasound (US), computed tomography (CT), and low-field magnetic resonance imaging (MRI) were used to explore venous tumor invasion in 86 patients with renal cell carcinoma. The findings confirm previous experience that ultrasound and dynamic CT are reliable methods for the evaluation of venous invasion. Low-field magnetic resonance imaging does not improve tumor staging. In case of equivocal findings, vena cavography should be used as a complementary examination.

Adult↗

Pharmacoangiographic assessment of penile arteries in patients with erectile failure.

Pudendal angiography was done before and thirty minutes after intracavernous administration of 6 mg phenoxybenzamine in 8 men in whom screening with ultrasound Doppler had revealed abnormalities of the penile blood flow. Five vessels were not visualized until phenoxybenzamine was given. Four vessels displayed marked dilatation, three had moderate and one had slight dilatation after the pharmacologic erection was provoked. The method appears to be of value to increase the quality of the angiographic examination.

Angiography↗

Maternal diabetes and its effect on biochemical and functional development of rabbit fetal lung.

The effect of maternal diabetes on functional and biochemical maturation of the fetal lung was studied in a rabbit model. Pregnancy was initiated only after diabetes had been established. Both the pregnant doe and its fetuses were hyperglycemic. For comparison, the fetal heart and liver were also studied. In the diabetic group, the DNA content was lower in the fetal heart and lung while the protein content was higher in all three tissues. The glycogen levels were higher only in the fetal lung. Glycogen synthase was higher in the fetal lung and heart while phosphorylase activity was higher in all three tissues from the diabetic group. The activities of key enzymes involved in glycolysis were not affected. No difference was observed in the concentration of total phospholipids or in the ability of the airway fluid to reduce surface tension. In contrast, fetal lungs from diabetic does did not expand as well as the controls and retained less air on deflation. These findings suggest that the utilization of glycogen in fetal lungs from the diabetic does was not complete and that the increased incidence of respiratory distress in infants of diabetic mothers may not be due to a lack of surfactant.

Animals↗

Studies on renal damage from percutaneous nephrolitholapaxy.

To study in detail the effects of percutaneous nephrolitholapaxy on renal function, a consecutive series of 11 patients were investigated preoperatively by excretory urography, gamma camera renography for determination of individual renal function and computerized tomography of the kidneys. Postoperatively, gamma camera examination, computerized tomography, antegrade nephrostography, renal angiography and excretory urography were performed. With 2 exceptions, percutaneous nephrostomy, dilation of the tract and stone removal were done in 1 stage with the patient under continuous epidural anesthesia. Nephrostomy tract dilation was done with an Olbert type balloon catheter or Alken metallic dilators. Thickening of Gerota's fascia was demonstrated by computerized tomography in most cases, and small to moderate perirenal hematomas were found in several. At gamma camera examination decrease of renal function was noted regularly on postoperative day 1 and returned to near initial levels 2 weeks postoperatively in most cases. Angiography in 10 patients showed discrete parenchymal scarring in some and a peripheral arteriovenous fistula in 1. We conclude that percutaneous renal stone surgery usually is tolerated well by the kidney.

Adult↗

Glucocorticoids and beta-adrenergic-receptor agonists: their combined effect on fetal rabbit lung surfactant.

In a previous study on pregnant rabbits (Am J Obstet Gynecol 1983; 147:437) we found that a prolonged infusion of the beta 2-adrenergic-receptor agonist terbutaline would first cause a release of fetal pulmonary surfactant, so that more was available in the airways. However, the airway fluid then contained less surfactant, indicating a depletion of stores. Since terbutaline is often used in high doses as a tocolytic agent, surfactant depletion could be a serious side effect. With further studies on rabbits, we wanted to test the hypothesis that with an accelerated surfactant synthesis, achieved with glucocorticoids, the increased release, evoked with the terbutaline, would never cause a depletion of the surfactant stores. Our results supported this hypothesis. Betamethasone, administered to the pregnant doe on the twenty-sixth and twenty-seventh days of gestation, 0.1 mg/kg, increased compliance of the fetal lungs, and more phospholipid phosphorus could be lavaged from the airways. These effects were further increased when, following steroid administration, the doe was infused with terbutaline. Depletion of the surfactant stores was never seen when betamethasone was given prior to the beta-adrenergic-receptor agonist.

Animals↗

Pulmonary surfactant release in fetal rabbits as affected by enprofylline.

At a gestational age of 27.9 days, pregnant rabbits were infused with enprofylline, a new xanthine with potent antiasthmatic effects. Following a priming dose of 5 mg/kg, 20 mg/kg were infused over a 2-h period. Controls received Ringer's solution. On completion of the infusion, the fetuses were delivered and the effect on their surfactant system was evaluated. Surface tension in airway fluid, measured with the pulsating bubble surfactometer, at minimal bubble size in the third cycle, was 21.8 +/- 2.2 (mean +/- SE) versus 28.7 +/- 1.7 mN m-1 (p less than 0.01). Pressure-volume loops of the lungs of the fetuses showed that at a deflation pressure 10 cm H2O the volume was 64 +/- 3 versus 49 +/- 3% (p less than 0.0005). The lung lavage fluid had a phospholipid phosphorus content of 41.9 +/- 3.8 versus 18.5 +/- 1.3 micrograms/g dry lung (p less than 0.0005). The values indicate that enprofylline caused a release of surfactant into the fetal airways.

Animals↗

Comparison between hemiarthroplasty and total hip replacement following failure of nailed femoral neck fractures focused on dislocations.

The frequency of and the factors causing dislocations of 71 total hip replacements and 59 hemiarthroplasties following failure of nailed femoral neck fractures were investigated. Dislocations were frequent and occurred in four (6%) and seven (l2%) hips respectively. Malposition of the components increased the risk for dislocation. A well-positioned total hip replacement was more stable than a hemiarthroplasty.

Aged↗

Blood supply of experimental liver tumors after intraarterial embolization with gelfoam powder and absolute ethanol.

Twenty rats with implanted liver tumor were studied. Following baseline angiography, the hepatic artery was embolized with Gelfoam powder or ethanol (n = 12) while the controls (n = 8) did not undergo embolization. Postmortem Microfil perfusion was performed in all livers. Filling of tumor lakes from the portal vein was seen more often in embolized animals than in controls, indicating the potential role of the portal venous system in the supply of dearterialized hepatic tumors. We discuss the possible clinical implication of our results.

Animals↗

Swanson implant arthroplasty of the wrist in rheumatoid arthritis.

Sixty rheumatoid wrists operated with Swanson implant arthroplasty were evaluated after a mean observation time of thirty-three months. Grip function in daily living improved in 60%, pain decreased in 88%, range of motion increased in 83% and grip strength increased in 69% of operated wrists. Significantly impaired function was found in wrists with implant fracture (12%) and in cases with pronounced bone resorption around the implant (23%). Ulnar deviation and carpal collapse were commonly found but did not impair the function significantly. In seven patients the contralateral wrist had been fused and was compared to the arthroplasty. The merits and indications of arthroplasty are discussed.

Adult↗

Silastic scaphoid implants in osteoarthritis of the radioscaphoid joint.

The long term results of Silastic Scaphoid implants are presented in two groups of patients, with osteoarthritis of the radioscaphoid joint without known history of trauma or established pseudarthrosis of the scaphoid bone. The patients in the former group all became satisfied with the operative result whereas in the latter group only half of the patients were satisfied with the result.

Adult↗

Occlusion of renal arterial tumor supply with absolute ethanol. Experience with 20 cases.

Absolute ethanol was used for renal ablation in 20 patients with carcinoma of the kidney. The procedure was followed by nephrectomy in 6 patients without metastases, all of whom are alive without evidence of recurrence 4 to 20 months after treatment. Fourteen patients with metastatic or locally advanced disease were embolized to palliate pain or hematuria without subsequent nephrectomy. Of these only 2 are alive, 4 and 18 months following the procedure. The technique for ethanol embolization is described and the use of occluding balloon catheters is recommended. No complications were encountered and the 'postembolization syndrome' was less marked as compared with a previous material of equal size where other methods of renal artery occlusion were employed. Microscopic examination in 10 of the infarcted kidneys showed extensive tumor necroses, sometimes with no evidence of viable tumor cells at all. Natural killer cell activity was determined before and 48 hours after renal artery occlusion in 7 patients, showing somewhat increased activity in 4. It is concluded that absolute ethanol is a safe, effective and easily administered agent for the infarction of renal tumors.

Adenocarcinoma↗