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Biomedical subjects

K Wada

Publications and source records attributed to K Wada.

At least 721 records · Page 40Linked to original sources
PubMed1984

Danazol binding to steroid receptors in human uterine endometrium.

To understand the mechanism of action of danazol, the binding of danazol to multiple classes of intracellular steroid binding proteins was studied in the human uterine endometrium. Danazol bound to endometrial receptors for estrogen, progesterone, and androgen and seemed to bind to endometrial intracellular corticosteroid-binding globulin and sex-hormone-binding globulin. Danazol occupies almost all binding sites of steroids in the steroid target cells in spite of the presence of endogenous steroids. It is speculated that the binding behavior of danazol may be related to its therapeutic effect on endometriosis.

Binding Sites↗
PubMed1984

Spirulina ferredoxin-NADP+ reductase. The complete amino acid sequence.

The amino acid sequence of ferredoxin-NADP+ oxidoreductase [EC 1.18.1.2, FNR] from Spirulina sp., a blue-green alga, was determined. Spirulina ferredoxin-NADP+ oxidoreductase was composed of 294 amino acid residues and the molecular weight of the holoenzyme was 34,135. An apparent homology of the amino(N)-terminal region was found between ferredoxin-NADP+ reductases from Spirulina and spinach. We also found some sequence similarities in human erythrocyte glutathione reductase and p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens, both of which are NADPH-dependent FAD enzymes.

Amino Acid Sequence↗
PubMed1984

Ferredoxins from the photosynthetic purple non-sulfur bacterium Rhodopseudomonas palustris. Isolation and amino acid sequence of ferredoxin I.

Two ferredoxins, ferredoxins I and II, were prepared from Rhodopseudomonas palustris. They were separated on a Sephadex column after carboxymethylation and ferredoxin I, the major component, was subjected to an amino acid sequence study. The protein was composed of 63 amino acid residues and the sequence was as follows: (sequence; see text). The molecular weight was calculated to be 6,718, excluding iron and sulfur atoms. The distribution of the nine cysteine residues was similar to but clearly distinct from those of ferredoxins of other photosynthetic bacteria. Comparison of this ferredoxin with those of other bacteria suggests that the photosynthetic bacteria evolved on separate lines. Ferredoxin II was also subjected to analyses of amino acid composition and terminal sequences, but no further study was possible due to the limited material. Although the composition was different from that of ferredoxin I, the terminal sequences were exactly the same as those of ferredoxin I.

Amino Acid Sequence↗
PubMed1984

Immunological study of heart diseases with special reference to the cytotoxicity of the heterophile antibody against cultured myocardial cells.

In our previous study, a high rate of "heterophile antibody" was found in idiopathic cardiomyopathy. In the present investigation the cytotoxic activity of this antibody was examined in cultured myocardial cells. Trypsin treated cells cultured for 3 days were used in the two-step method of the complement dependent cytotoxicity test. The trypan blue dye-exclusion method was utilized to determine the percentage of surviving cells, and the cytotoxic effect was expressed by the cytotoxic index (CI). With fresh rabbit serum as complement, the mean CI value of the heterophile antibody positive sera was 20.7, which was in contrast to the value of anti-heart antibodies negative sera (p less than 0.01). As the cytotoxic effect to the heterophile antibody was absent without the complement, it may be complement dependent. Thus, our results suggest that the heterophile antibody may play a role in the pathogenesis or be an incremental factor of idiopathic cardiomyopathy.

Animals↗
PubMed1984

[Afterloading interstitial brachytherapy by Ir-192 seed assembly for malignant brain tumor].

We developed and applied interstitial after-loading endocrine therapy as a cancer treatment modality. Between Sept. 1982 and May 1983, 5 patients with malignant brain tumors were treated using the removable after-loading Iridium-192 seed assembly implant technique. 4 of the 5 patients experienced recurrent gliomas after the operation alone or in combination with external post-operative radiotherapy. In 3 patients, local control was achieved and has been maintained for more than 11 months. Our preliminary brachytherapy procedures for the treatment of malignant brain tumors are described.

Adenocarcinoma↗
PubMed1984

[A case of primary adenosquamous carcinoma of the stomach associated with gastric polyposis].

We report a 76-year-old woman with Borrmann 3 carcinoma associated with gastric polyposis, who underwent total gastrectomy and splenectomy. Histopathological study of resected specimens revealed squamous cell carcinoma in some sections keratinization was seen; other sections evidenced adenocarcinoma. Due to intra- acinous epithelial stratification and platyform development, the adenosquamous carcinoma was considered to have originated from squamous epithelial metaplasia of the adenocarcinoma cells. Of 90,639 patients who had undergone resection to treat gastrocarcinoma, 236 (0.26%) had adenosquamous carcinoma. However, only 7 cases were associated with polyposis.

Adenocarcinoma↗
PubMed1983

Dephosphorylation and activation of chicken liver acetyl-coenzyme-A carboxylase.

Studies were made on the dephosphorylation and activation of chicken liver acetyl-coenzyme-A carboxylase. The enzyme isolated by avidin-Sepharose affinity chromatography in the presence of protein phosphatase inhibitors contained 4.9 +/- 0.2 mol of alkali-labile phosphate/mol subunit and had a specific activity of 3.5 +/- 0.4 units/mg protein. The purified enzyme was dephosphorylated and activated concomitantly when incubated in the presence of protein phosphatase with a release of approximately 2 mol of phosphate/mol subunit. Limited tryptic digestion of the native and dephosphorylated forms of the enzyme (Mr 220 000) containing 4.9 and 2.9 mol of phosphate/mol of subunit, respectively, gave almost quantitatively similar polypeptides of Mr 215 000 containing 4.0 mol and 3.0 mol of phosphate per mol, respectively, which were indistinguishable by dodecylsulfate-polyacrylamide gel electrophoresis. A peptide of Mr approximately 5000 was lost from both enzymes. This result suggests that at least one of the two protein-phosphatase-labile phosphorylation sites is sensitive to trypsin. The native enzyme and those modified by protein phosphatase or by limited tryptic digestion exhibited a bi-phasic dependence on citrate. Activation of the native enzyme by protein phosphatase occurred at all the concentrations of citrate used. However, activation of the enzyme by limited tryptic digestion was found at concentrations greater than 5 mM citrate. The dephosphorylation by protein phosphatase caused an approximately fivefold activation in the enzyme activity when assayed at physiological concentrations of citrate (0.5-2.0 mM).

Acetyl-CoA Carboxylase↗
PubMed1983

Trophic effect of iron-bound transferrin on acetylcholine receptors in rat skeletal muscle in vivo.

Trophic effect of iron-bound transferrin (FeTf) on the total content of acetylcholine receptors (AChRs) and the specific activity of AChRs in innervated and denervated skeletal muscle was investigated in vivo. The right ischiadic nerves of 15 rats weighing 160 g were transected. FeTf (1.2 mg/ml) was injected daily into bilateral crural muscles of rats for the following 11 days. Control groups received injections of saline or no treatment. FeTf significantly increased the total content of AChRs and the specific activity of AChRs in innervated and denervated muscle compared with control groups (P less than 0.001). This result shows that intramuscular injections of FeTf may be useful for the treatment of disorders of neuromuscular transmission.

Animals↗
PubMed1983

Serum mitochondrial aspartate aminotransferase in patients with polymyositis.

The clinical significance of serum aspartate aminotransferase (GOT) isozymes was studied in 18 patients with polymyositis. Abnormally high levels of mitochondrial GOT (mGOT) (6.2 +/- 1.2 IU/L, mean +/- SEM; normal, less than 2.0 IU/L) and cytosol GOT (sGOT) (95 +/- 21.6 IU/L; normal, less than 25 IU/L) were observed in sera. In polymyositic muscles, the sGOT level was significantly decreased but mGOT was not. The levels of serum sGOT and mGOT and the ratio of mGOT/tGOT before corticosteroid therapy correlated well with the severity of muscle weakness. Serial determination of CPK, sGOT, and mGOT during corticosteroid therapy revealed that mGOT most rapidly returned to normal. Exercise did not increase serum mGOT in polymyositis.

Adrenal Cortex Hormones↗