Search PubMed⌕ Search

Biomedical subjects

K Ueda

Publications and source records attributed to K Ueda.

At least 1,351 records · Page 75Linked to original sources
PubMed1985

Modified Blalock-Taussig shunt following long-term administration of prostaglandin E1 for ductus-dependent neonates with cyanotic congenital heart disease.

In the treatment of cyanotic heart diseases in neonates who are ductus dependent, the small size of the pulmonary artery and/or subclavian artery often precludes construction of a sufficient Blalock-Taussig shunt. To avoid an emergency operation and to obtain pulmonary and subclavian arteries of adequate size (3 mm), a low dose of prostaglandin E1 was given to 16 neonates for a mean of 47 days (range 26 to 65 days), and a modified Blalock-Taussig shunt operation was performed in neonates whose mean age was 50 days. The pulmonary artery grew significantly (mean 4.5 mm) and the mean diameter of the subclavian artery was 3 mm, which was large enough for a good shunt at the time of operation. There were two late deaths and one operative death. All surviving patients shunts at the mean follow-up period of 22 months. The shunt was constructed on the same side as the ductus arteriosus so that the ductus could be ligated at the same operation in selected cases; the ductus may not close after discontinuation of prostaglandin E1 infusion and may cause uncontrollable congestive heart failure because of excessive pulmonary blood flow, particularly in patients with a univentricular heart. The ductus, dissected in nine patients, was enlarged and elongated in all and was ligated in four patients without complication.

Ductus Arteriosus, Patent↗
PubMed1985

Immunological responses to monoassociated Bifidobacterium longum and their relation to prevention of bacterial invasion.

After monoassociation of Bifidobacterium longum with germ-free BALB/c mice (nu/+, heterozygous to nu gene), B. longum was recovered (10(2)-10(4) viable units per organ) from the mesenteric lymph nodes, liver and kidneys for 2 weeks post intragastric inoculation, but not after 4 weeks and later. Cessation of bacterial translocation was not observed in monoassociated nude (nu/nu) mice. Anti-B. longum IgA antibody was detected by ELISA using B. longum cell wall antigen in bile from Week 1 and in ileac wall extract from Week 8 post-association in both nu/+ and nu/nu mice. Total IgA levels in bile, ileac wall extract and caecal contents were also elevated in both mice after monoassociation. Cell-mediated immunity measured by the footpad test and macrophage migration inhibition test using B. longum protein fraction was detected in nu/+ mice in Week 4 and later, but not in nu/nu mice. Nu/nu mice reconstituted 4 weeks prior to monoassociation with lymphocytes from flora bearing nu/+ mice developed delayed footpad reactivity and bacterial translocation stopped after 4 weeks. Cell-mediated immunity rather than IgA antibody correlated well with the cessation of translocation.

Actinomycetales Infections↗
PubMed1985

[A clinical evaluation of aspoxicillin in children].

Eighteen infants and children with infectious diseases were treated with aspoxicillin (ASPC), a new semisynthetic penicillin. The result was as follows: The clinical responses to ASPC were excellent in 6 patients and good in 5 patients of 11 children with bacterial infections. The bacteria isolated from the culture of throat swab and urine in 5 patients were Streptococcus pneumoniae, Escherichia coli, and Staphylococcus aureus which were all eradicated by the treatment of ASPC. The mean serum concentration of ASPC reached the peaks of 73.3 micrograms/ml in 5 cases with dose of 20 mg/kg, and 136.3 micrograms/ml in 3 cases with dose of 40 mg/kg 15 minutes after the intravenous administration. The mean half-lives of ASPC in the serum were 1.08 hours for the dose of 20 mg/kg and 1.07 hours for the dose of 40 mg/kg. The mean urinary recoveries of ASPC in 6 hours following the intravenous administration were 73.7% in 3 cases with dose of 20 mg/kg, and 79.6% in 1 case with dose of 40 mg/kg. No clinical side effect of ASPC was observed. An increase of platelet was noticed in a child with infectious mononucleosis in the course of administration of ASPC.

Amoxicillin↗
PubMed1985

[Assessment of response to therapy based on the application of the guideline of reporting treatment results of head and neck cancer].

It seems difficult to compare the treatment results to each other, without "common language" to describe cancer therapy and its results. In this paper, guideline of reporting treatment results of head and neck cancer was applied to carcinoma of the pharynx and larynx. There were some problems in reporting of response, such as the measurability and assessment of the response. However, there were small discrepancies between assessment of response to therapy and end-point evaluation, i.e., long-term control, survival and disease-free survival, according to the prospective department-based and retrospective hospital-based studies.

Head and Neck Neoplasms↗
PubMed1984

Modified Blalock-Taussig shunt operation without cardiac catheterization: two-dimensional echocardiographic preoperative assessment in cyanotic infants.

Between May 1981 and December 1983, 25 infants with cyanotic congenital heart defects underwent 26 Blalock-Taussig shunt operations without cardiac catheterization and angiocardiography. In these infants, the diagnosis was established by 2-dimensional echocardiography (2-D echo) supplemented with clinical findings, chest x-ray and electrocardiography. The right and left pulmonary artery measurements, a prerequisite for a shunt operation, determined by 2-D echo were compared with those at surgery and had an excellent correlation (r = 0.94). No infant died as a consequence of an early shunt failure, and only 1 died of postoperative pyothorax. In conclusion, 2-D echo may eliminate the need for invasive investigation in selected patients undergoing the Blalock-Taussig anastomosis.

Blood Vessel Prosthesis↗
PubMed1984

Rhythmic discharge of mitral cells in the carp olfactory bulb.

We examined conditions in which rhythmic discharges of the mitral cell are obtained in the carp olfactory bulb. When the mean firing rates of the responses to various concentrations of NaCl solutions were high (more than 5 Hz), rhythmic discharges appeared. Results provide experimental support for the rhythm generation hypothesis on the dendrodendritic synapse in the vertebrate olfactory bulb.

Animals↗
PubMed1984

A field-potential study of centripetal and centrifugal connections of the olfactory bulb in the carp, Cyprinus carpio (L).

The projection areas of mitral cell axons in the lateral (LOT) and medial (MOT) olfactory tracts and the pathways of two groups of centrifugal fibers (with fast and slow conduction velocities) to the olfactory bulb were studied in the carp using the field potential method. Mitral cell axons of the LOT and those of the MOT projected to the overlapping areas in the bilateral telencephalon and preoptic area (POA), the contralateral MOT, and the nucleus posterior tuberis (NPT) in the diencephalon. However, the precommissural, ventromedial part of the ipsilateral telencephalon received the projection only from mitral cell axons of the MOT, not from those of the LOT. No projection was detected in the contralateral LOT and the contralateral olfactory bulb. The distribution of the fast and slow centrifugal fibers and/or their cells of origin were similar to each other. They were distributed bilaterally in the telencephalon and POA, in the NPT, and contralateral LOT and MOT. These areas overlapped with the projection areas of the mitral cell axons considerably. The results support and extend those obtained from anatomical studies.

Animals↗
PubMed1984

Sequence-specific DNA damage induced by reduced mitomycin C and 7-N-(p-hydroxyphenyl)mitomycin C.

Mitomycin C reduced with sodium borohydride induced the DNA damage at deoxyguanosines preferentially in dinucleotide sequence G-T. The DNA damage produced strand breaks when subsequently heated. The DNA damage scarcely occurred when the end-labeled DNA was preincubated with ethidium bromide or actinomycin D before the addition of mitomycin C and the reducing agent. Fully reduced mitomycin C did not induce the DNA damage. The mitomycin C-inducing DNA damage seems to require the intercalation of the partially reduced mitomycin C of short life time, probably semiquinone radical, between DNA base pairs. The inhibitory effects of sodium chloride and radical scavengers suggested that the requirement of the covalent bond formation of mitomycin C to DNA and the involvement of oxygen radicals in the DNA damage. 7-N-(p-hydroxyphenyl)mitomycin C, which is reported to show a higher antitumor activity and a lower toxicity than mitomycin C, was readily reduced with dithiothreitol and induced the sequence-specific DNA damage, whereas mitomycin C was not.

Bacteriophage phi X 174↗
PubMed1984

Sequence specificity of heat-labile sites in DNA induced by mitomycin C.

The sequence specificity of the mitomycin C-DNA interaction was directly determined by using DNA sequencing techniques and by using 3'- or 5'-end-labeled DNA fragments of defined sequence as substrates. Mitomycin C reduced with sodium borohydride induced heat-labile sites in DNA preferentially at specific sequences. The heat-labile sites were induced most preferentially at the dinucleotide sequence G-T ( especially Pu G-T), which was determined by scanning autoradiograms with a microdensitometer after gel electrophoresis. DNA was cleaved at the 3' side of deoxyguanosines and of some deoxyadenosines by heat treatment. Oligonucleotides produced by heat treatment after reaction with reduced mitomycin C contained phosphoryl groups at the 5' termini. The 3' termini seemed not to have simple structures, judging from their electrophoretic mobilities. Oxygen radicals such as singlet oxygen and hydroxyl radical were possibly involved in the induction of heat-labile sites.

Antibiotics, Antineoplastic↗
PubMed1984

A new procedure for determining thymine residues in DNA sequencing. Photoinduced cleavage of DNA fragments in the presence of spermine.

A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal lamp emitting mainly 254-nm light in the presence of 1 M spermine in distilled water resulted in a T specific cleavage of the DNA chains. This method does not require piperidine treatment. By contrast, when the DNA fragments were irradiated in the presence of methylamine under similar conditions, both G and T bands with the intensity of G greater than T have appeared. A similar but less selective T cleavage has also been observed in the irradiation of 5'-[32P]-end-labeled DNA fragments in the presence of spermine followed by brief heating of the photolysate in a loading buffer for gel electrophoresis. The T specific photoreaction with spermine and the G greater than T reaction with methylamine described here may be conveniently used in combination with the standard Maxam-Gilbert's reactions to provide independent confirmatory readings.

Autoradiography↗
PubMed1984

ADP-ribosyl protein lyase. Purification, properties, and identification of the product.

ADP-ribosyl protein lyase, formerly termed ADP-ribosyl histone-splitting enzyme (Okayama, H., Honda, M., and Hayaishi, O. (1978) Proc. Natl. Acad. Sci. U. S. A. 75, 2254-2257), was purified approximately 4,000-fold from rat liver and characterized. The purified enzyme exhibited a single protein band at the position of Mr = 83,000 upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme split the bond between ADP-ribose and histone H2B or H1, and also acted on ADP-ribosyl pentapeptide (Pro-(ADP-ribosyl)Glu-Pro-Ala-Lys) of H2B but not its deadenylylated derivative, phosphoribosyl pentapeptide. The enzyme cleaved the bond between histone and mono(ADP-ribose), but hardly cleaved the bond with oligo- or poly(ADP-ribose). The enzymatic product was close to, but not identical with, ADP-ribose. The terminal ribose residue, obtained by hydrolysis of the split product by snake venom phosphodiesterase and alkaline phosphatase, was identified as 3-deoxy-D-glycero-pentos-2-ulose by the following gas chromatography-mass spectrometric analyses: 1) the reduced sugar was a mixture of 3-deoxy-threo- and 3-deoxy-erythro-pentitol, and 2) the deuterated reduced sugar was identical with that derived from synthetic 3-deoxy-D-glycero-pentos-2-ulose. This result indicated that the direct product was 5'-ADP-3"-deoxypent-2"-enofuranose, a dehydrated form of ADP-ribose, and that the enzyme is a lyase and not a hydrolase.

Adenosine Diphosphate Ribose↗
PubMed1984

Genetic variants of protease inhibitors against fungal protease and alpha-chymotrypsin from hemolymph of the silkworm, Bombyx mori.

Many electrophoretic variants of hemolymph inhibitors of proteases from Aspergillus melleus and pancreatic alpha-chymotrypsin were found using 126 silkworm strains. Six inhibitors of the fungal protease were detected and eight of chymotrypsin; the distribution of inhibitors among Japanese, Chinese, and European races was investigated. Comparison of electrophoretic patterns from F1 hybrids and parents showed that the offspring produce inhibitors of both parental types. Segregation in F2 and backcrossing suggest that the expression of each inhibitor is controlled in most cases by a pair of alleles which are responsible for strong and null bands. Two bands of fungal protease inhibitors C and D were controlled by codominant alleles. These results suggest that polymorphism of hemolymph protease inhibitors in the silkworm would be a useful experimental system for the study of the genetic control of protease inhibitors.

Animals↗
PubMed1984

Natural history of borderline hypertension in the Hisayama community, Japan--I. The relative prognostic importance of transient variability in blood pressure.

Long-term prognosis of borderline hypertensives was studied in a prospective population survey carried on since 1961 in the town of Hisayama, Japan. Five consecutive BP recordings on 1621 subjects aged 40 and over were obtained at entry, and the variability in BP between the first and fifth readings was taken into account when classifying the subject into categorical groups. Even with an estimated variability in BP in several measurements on one occasion, a large fluctuation in BP was observed in both the borderline hypertensives and the normotensives. Borderline hypertensives with a transient elevation in BP more frequently died from cardiovascular disease than did those without BP elevation, as estimated by the long-term cumulative mortality. However, there was no difference in the frequency of hypertension-related organ damage between these two groups at entry.

Adult↗
PubMed1984

Telencephalic and preoptic areas integrate sexual behavior in hime salmon (landlocked red salmon, Oncorhynchus nerka): results of electrical brain stimulation experiments.

Various patterns of sexual behavior were evoked in freely swimming hime salmon by electrical stimulation of specific loci in the telencephalon and the preoptic area (POA) using chronically implanted electrodes. Furthermore, co-ordinated sexual behavior corresponding to stages of the natural spawning sequence was elicited from some of these brain regions. These results suggest that (1) sexual behavior is integrated in specific parts of the telencephalon and POA, and (2) within these regions there is a hierarchy of neural systems which mediate progressively more complete components of normal sexual behavior.

Animals↗