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Biomedical subjects

K Ueda

Publications and source records attributed to K Ueda.

At least 1,315 records · Page 73Linked to original sources
PubMed1985

Hemolytic anemia following postnatally acquired rubella during the 1975-1977 rubella epidemic in Japan.

Hemolytic anemia following postnatally acquired rubella first was reported by Sato et al. in 1977. Thirteen cases of hemolytic anemia (including two cases of hemolytic uremic syndrome) following postnatally acquired rubella infection reported in a nationwide epidemic of rubella in Japan in 1975-1977 are reviewed in this article, and another case is added. Clinical symptoms of hemolytic anemia occurred 2 to 6 days from the onset of rubella rash. Direct Coombs test was positive in three of the 11 cases tested, and the indirect test was positive in two of the 13 cases. Hemolytic anemia should be considered as a complication of postnatally acquired rubella, though to date, it has only been reported in Japan.

Adult↗
PubMed1985

A luteotrophic action of prolactin: suppression of intraluteal prostaglandin production or effect?

Adult rats were hypophysectomized and their pituitary glands autotransplanted on day 2 of an estrous cycle (day 1, ovulation); the rats were either hysterectomized or sham hysterectomized immediately before this operation. On day 21, a few days after the beginning of the 3+ month period of regression of progesterone secretion, they were injected sc with 500 micrograms prostaglandin F2 alpha (PGF2 alpha) either on day 21 alone, on days 21 and 22, on days 21, 22, and 23, or on days 21 and 23; controls were injected with saline on days 21, 22, and 23. In the latter the serum progesterone level slowly fell, as it does in untreated rats subjected to pituitary autotransplantation on day 2. PGF2 alpha treatment induced a rapid, drastic fall in the progesterone level, but in each group except the one injected on days 21 and 23, the level returned to, or close to, the control level in about 10 days. Even in the group injected on days 21 and 23, the pattern of serum progesterone resembled the others although the values were much lower. In a similar group of rats subjected to pituitary autotransplantation on day 2 (all of which were also hysterectomized on day 2), a single injection of 500 micrograms PGF2 alpha (which again induced the same changes in serum progesterone as in the first experiment) was combined with either a 6-h or 4-h withdrawal of PRL; this was achieved by injecting 2 bromo-alpha-ergocryptine (CB-154) at zero time to lower PRL secretion, and beef PRL, in a delaying vehicle, 6 or 4 h later, followed by a second dose of PRL 18 h after the first. When combined with such a brief period of PRL withdrawal, the PGF2 alpha treatment induced a very rapid, drastic and permanent fall in serum progesterone, equal to that induced by CB-154 alone, or by removing the pituitary transplant on day 21. The brief periods of PRL withdrawal, by themselves had very little (6 h) or no (4 h) effect on the progesterone level, and treatment with PRL alone was also without effect. Treatment with CB-154, PGF2 alpha, and PRL simultaneously (i.e. without a period of PRL withdrawal) had the same effect as treatment with PGF2 alpha alone.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1985

Ultrastructural localization of actin in nuclear matrices from mouse leukemia L5178Y cells.

We examined the distribution of actin in isolated nuclear matrices from mouse leukemia L5178Y cells using an anti-actin antibody and protein A-conjugated colloidal gold particles. Before immunogold staining, we partially digested the surface lamina of the nuclear matrix with trypsin (Nakayasu and Ueda, Exp. Cell Res. 143, 55-62, 1983) to allow penetration of the gold particles into the nuclear matrix. Trypsin digestion slightly modified the internal structure of the nuclear matrix, but did not affect the actin content in the nuclear matrix nor the reactivity of actin with the antibody. Many colloidal gold particles were present along fibrogranular structures in the nuclear matrix. The results reported here confirm the existence of actin in the interior of the nuclear matrices of L5178Y cells.

Actins↗
PubMed1985

Blood pressure tracking in Japanese adolescents. Five-year follow-up in Hisayama, Japan.

Blood pressures (BPs) were measured with standardized sphygmomanometers in 434 Japanese boys and girls living in the town of Hisayama. Simultaneously, data on pulse rate, weight and height were obtained. Out of the original 434 subjects, data were obtained repeatedly for 5 years in 280 subjects. BP levels were significantly correlated with weight in those aged 14-15 and also 19-20 years, but correlation coefficients were small. During the 5-year period, the mean systolic and diastolic blood pressure (SBP & DBP) increased significantly in both sexes, but the increments were greater in boys. Both SBP and DBP at 14-15 years of age were significantly correlated with data taken 5 years later for both sexes, and subjects with a higher initial BP (more than 90th percentile of the distribution) tended to have a higher BP after 5 years. SBPs after 5 years were independently correlated with initial SBP levels and changes in QI (D-QI) in both sexes. On the other hand, DBPs after 5 years were independently correlated with initial DBP levels and height for boys, and initial DBP levels and D-QI for girls.

Adolescent↗
PubMed1985

Spontaneous arthritis in nude rats of Rowett hooded strain.

Spontaneous arthritis was found in 19 of 55 Rowett hooded strain rats with rnu gene. Most cases were in the male rnu/rnu (15/19) but a few occurred in the male rnu/+ (3/10) and female rnu/rnu (1/8). The lesions were first noted as reddened swelling due to exudative inflammation of periarticular soft tissues including synovial membranes in the tarsal and/or carpal joints. Most of the affected animals cured leaving slight induration but in a few male rnu/rnu ankylosis with pannus formation and exostosis developed. No sign of mycoplasmal or bacterial infection was noticed in the colony.

Age Factors↗
PubMed1985

Bleomycin cleaves DNA depending on DNA primary, secondary, and tertiary structures.

The cleavage by bleomycin-Fe(II) complex in the presence of dithiothreitol was investigated by using 3'- or 5'-end-labeled DNA containing the region of the bacteriophage G4 origin of complementary strand synthesis as substrates. Bleomycin cleaved single-stranded DNA substrates preferentially at inverted repeat sequences, which potentially form stem-and-loop structures, in addition to the primary sequence specificity previously reported. DNA sequences preferentially cleaved in the double-stranded substrate were resistant when they lay outside the stem regions. These results suggest the formation of three predicted stem-and-loop structures and other possible secondary structures near the replication origin. Changes of the degree of bleomycin-induced DNA cleavage in a NaCl concentration between 0 and 50 mM suggest that a subtle change of ionic conditions within the double helix, or of DNA conformation, or of both, may occur at 0-50 mM NaCl. Bleomycin appears to be a useful reagent for analyzing secondary and tertiary structures of DNA.

Base Sequence↗
PubMed1985

[Clinical evaluation of Cernilton on benign prostatic hyperplasia].

Twenty-two patients whose average age was 67 years and who had benign prostatic hyperplasia of stage I and II were treated with Cernilton for more than 4 weeks. Subjective symptoms were excellently improved and the improvement rate was over 85% in all of the evaluated symptoms of dysuria. In the overall evaluation, 18 out of 22 patients were rated as moderately improved or better, 2 were slightly improved and 2 remained unaltered. Aggravation of the symptoms was found in none of the patients. Objective findings such as residual urine volume and urinary flow rate were improved in 3 patients, although the shrinkage of the prostate was not observed on rectal palpation, retrograde urethro cystography or transrectal ultrasonography. No adverse reaction was observed during Cernilton therapy. In conclusion, it is suggested that Cernilton may be effective and safe for the conservative treatment of patients with early stage prostatic hyperplasia of non-surgical indication.

Aged↗
PubMed1985

[Mitral valvular disease secondary to mitral ring calcification: a clinicopathologic study].

Fifteen cases with mitral valvular disease caused by mitral ring calcification (MRC) were observed among 2,800 consecutive autopsy patients more than 60 years of age. They consisted of one man and 14 women whose average age was 86.4 years. All had been diagnosed as having mitral valvular disease during life. For this clinicopathologic study, the cases were categorized as nine cases with mitral regurgitation (MR Group) and six with mitral stenosis (MS Group). Among the 15 cases, phonocardiograms were obtained in 14 and echocardiograms in 6. In addition, 122 cases with MRC, the length of which was 5 mm or more, were selected from 900 recent consecutive autopsies of senile patients, to evaluate the site of calcification and to analyze the ratio of calcification length to mitral valve ring circumference. The following conclusions were obtained: The prevalence of mitral valvular disease due to MRC in the aged was 15/2,800 (0.5%). MR was observed in nine cases and MS in six. Phonocardiograms of the MR Group revealed a holosystolic murmur in seven cases, a late systolic murmur in one, a third heart sound in four and a fourth heart sound in five. In the MS Group, a holosystolic murmur was found in four, a presystolic murmur in four, a diastolic rumble in one, but no opening snap in any case. A diamond-shaped systolic murmur was found in nine cases with MS or MR, suggesting an ejection systolic murmur caused by an associated calcified aortic valve. Echocardiograms showed markedly decreased DDR in five cases and increased echo intensity of the aortic valve in four. Pathologic findings revealed that the mean length of MRC was 36.6 mm in the MR Group and 58.0 mm in the MS Group. The calcification ring ratio (CRR = MRC/MVR X 100) was 50.3% in the MR Group and 69.8% in the MS Group. In the MR Group, MRC involved the anterolateral commissure in three, posteromedial commissure in five, and both in one. In five of six cases with MS, both commissures were involved by MRC. The study of 122 cases with MRC length greater than or equal to 5 mm suggested that MRC occurred first in the middle scallop of the posterior mitral leaflet, and extended to the posterior scallop, subsequently extending up to the anterior scallop, and finally involved the anterior mitral leaflet beyond the commissures.(ABSTRACT TRUNCATED AT 400 WORDS)

Aged↗
PubMed1985

[Tricuspid regurgitation after corrective surgery of ventricular septal defect: evaluation using pulsed Doppler echocardiography].

Using two-dimensional (2DE) and pulsed Doppler echocardiography (PDE), the prevalence, cause, and 2DE findings of tricuspid regurgitation (TR) occurring after corrective surgery for ventricular septal defect (VSD) were investigated. Fifty-one postoperative patients (25 boys and 26 girls) ranging in age from 2 months to 11 years and 9 months were studied. There were 23 preoperative patients with VSD and 26 children without cardiac disorders who served as controls. TR was diagnosed when pansystolic abnormal Doppler signals were obtained in the right atrial cavity by PDE. Twenty-three (45.1%) of the 51 postoperative patients, two (8.7%) of 23 preoperative patients, and none of 26 normal subjects were found to have TR. The prevalence of TR in postoperative patients was significantly (p less than 0.01) greater than those of the two control groups, but there was no significant difference between the control groups. The prevalence of TR was not relevant to sex or type of VSD, but TR was detected more frequently in patients with residual shunts (p less than 0.01), in those with postoperative pulmonary hypertension (p less than 0.01), and in those with morphological abnormalities of the tricuspid valve (p less than 0.01). In postoperative patients, the mean age at surgery of those with TR (mean +/- SD = 18.3 +/- 17.4 months) was younger than that of those without TR (29.9 +/- 21.4), and the cardiothoracic ratio (0.59 +/- 0.07 and 0.55 +/- 0.07, respectively), tricuspid annulus size (122 +/- 19 and 104 +/- 10% of normal), and right atrial size (143 +/- 34 and 106 +/- 12% of normal) were greater in patients with TR. The maximal extent of the TR signal correlated positively with right atrial size and tricuspid annulus size, and seemed to be a useful index for evaluating the severity of TR. Abnormal morphological findings of the tricuspid valve detected by 2DE were (1) no coaptation of the anterior and septal leaflets in systole (= discoaptation), (2) no coaptation of both leaflets throughout the cardiac cycle (= valve defect), and (3) apical displacement of the valve in systole (= bulging). (1) and (2) appeared to be associated with TR produced by operative injury; and (3), with functional TR. It was concluded that TR occurs frequently after corrective surgery for VSD, and 2DE and PDE were useful for evaluating the cause and severity of TR.

Child↗