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Biomedical subjects

K Ueda

Publications and source records attributed to K Ueda.

At least 1,297 records · Page 72Linked to original sources
PubMed1985

Left ventricular systolic function in children with coronary arterial lesion following Kawasaki disease.

Seventy-five children with Kawasaki disease underwent quantitative left ventricular cineangiography, and angiographic measurements were made to obtain left ventricular ejection fraction (LVEF) and mean velocity of circumferential fiber shortening (mVcf). In addition, left ventricular pressure and echocardiograms were taken simultaneously before and after nitroprusside infusion using a micromanometer. In the present study, the slope of the line describing end-systolic pressure-diameter relations (Emax) was calculated. The 75 children with Kawasaki disease were classified into five groups according to the severity or condition of associated coronary lesions: Grade 0 group (normal--no significant enlargement of the coronary artery is seen), grade 1 group (mild state of lesion--the maximum diameter of the coronary artery is 4.0 mm or less), grade 2 group (moderate state of lesion--the maximum diameter of the coronary artery is 4.0-8.0 mm), grade 3 group (serious state of lesion--the maximum diameter of the coronary artery is 8.0 mm or over, i.e., giant aneurysm), and stenotic lesion (SL) group (myocardial infarction or various other stenotic lesions are involved). Each group was investigated for each index of left ventricular function. In the grade 2 group, the averaged value of LVEF was slightly smaller than those for the grade 0 and 1 groups, while in the grade 3 and SL groups, the averaged values of LVEF were markedly smaller than those for all other groups. Grade 3 and SL groups showed averaged mVcf values that were smaller than those for other groups, while grade 2-3 and SL groups showed averaged values of Emax that were markedly smaller than those for the remaining groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Aneurysm↗
PubMed1985

Association of rapidly-labelled RNAs with actin in nuclear matrix from mouse L5178Y cells.

More than 90% of rapidly-labelled nuclear RNA was associated with a nuclear matrix prepared from mouse leukemia L5178Y cells. The binding was not affected with up to 4 M NaCl; however, these RNAs were released from the nuclear matrix by treatment with a low ionic strength buffer (5 mM Tris-HCl buffer, pH 7.5, containing 1 mM ATP, 1 mM dithiothreitol, 0.2 mM ethylenediaminetetraacetic acid (EDTA) and 0.4 mM calcium chloride), without destruction of the sphere of the nuclear matrix. Actin filaments in the nuclear matrix were depolymerized with this buffer accompanied with rapidly-labelled RNAs. When the depolymerization was inhibited by slight modifications of the low ionic strength buffer (replacement of ATP by the same concentration of GTP; replacement of calcium ion by the same concentration of magnesium ion; addition of 20 micrograms/ml of phalloidine, which is a specific inhibitor of actin depolymerization), the release of rapidly-labelled RNAs from the nuclear matrix was also inhibited. The complex containing rapidly-labelled RNAs and matrix proteins was solubilized by a sonication from the nuclear matrix, and subjected to cesium chloride equilibrium centrifugation. Rapidly-labelled RNAs were concentrated on the bottom of the gradient accompanied with a small number of proteins (68K, 60K, 43K and 40K). The 43K protein was identified as actin by immunoblotting. By RNase digestion before equilibrium centrifugation, actin in the bottom fractions disappeared. These results suggest that rapidly-labelled RNAs anchor on the actin filaments in the nuclear matrix.

Actins↗
PubMed1985

Diadenosine tetraphosphate as a signal molecule linked with the functional state of rat liver.

Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A), which has been suggested to be a signal nucleotide involved in positive growth regulation, was determined in rat liver under various conditions using the luciferin/luciferase system. The Ap4A level was 920 pmol/g of wet liver in normal adult rats, but was high at early stages of growth, the maximum value being 1460 pmol/g in 3-wk-old rats. An increase in Ap4A levels preceded deoxyribonucleic acid synthesis in liver after hepatectomy. Ap4A levels were increased 5.5-fold (5125 pmol/g) 24 h after hepatectomy. The range of such increases was closely correlated with the mass of liver removed. Cholestasis produced by bile duct ligation and intraperitoneal injection of D-galactosamine remarkably lowered the Ap4A level to 20% and 42%, respectively; however, the adenosine triphosphate level was only slightly decreased to 65% and 78%, respectively. Fourteen days after bile duct ligation, Ap4A levels fell to less than 220 pmol/g, and the rats could no longer survive partial hepatectomy. These results suggest that hepatic Ap4A levels correlate positively with regenerative activity.

Adenine Nucleotides↗
PubMed1985

Deficiency of serum cholesteryl-ester transfer activity in patients with familial hyperalphalipoproteinaemia.

Lipoprotein patterns and cholesteryl ester transfer activity (CETA) were examined in 2 patients with familial hyperalphalipoproteinaemia (FHALP). The proband was a healthy 58-year-old Japanese male who had an HDL cholesterol of 7.83 mmol/l (301 mg/dl). His sister's HDL cholesterol was 4.52 mmol/l (174 mg/dl), which suggested that both were homozygous carriers of FHALP. In both subjects HDL showed a high cholesterol/apo A-I ratio and appeared to be a larger-sized particle than normal HDL on agarose gel chromatography. Two of the proband's children showed higher HDL cholesterol levels (1.74 mmol/l, 2.16 mmol/l) than normal, but another 2 children showed normal levels (1.48 mmol/l, 1.40 mmol/l). However, the ratios of HDL cholesterol to total cholesterol and to apo A-I in all children were higher than normal. These data suggest, but do not prove, that all his children were heterozygotes. Apo B levels in all of the family members studied were lower than normal (47-80 mg/dl). Deceased members of the same family had not died from cardiovascular disease. Cholesteryl-ester transfer activity was studied in both patients. When serum or lipoprotein deficient serum (d greater than 1.21) and [3H]cholesteryl ester labelled HDL3 were incubated in the presence of an LCAT inhibitor, there was no evidence of cholesteryl ester transfer from HDL to VLDL and/or LDL, unlike normal subjects. The deficiency of CETA in these patients with FHALP presumably accounted for the increase in particle size and cholesterol enrichment of HDL.

Adult↗
PubMed1985

An electromyographic analysis of electrically-evoked prey-catching behavior by means of stimuli applied to the optic tectum in the Japanese toad.

As a step toward elucidating the tectal-controlling functions for generating the prey-catching motor pattern, electrically-evoked "snapping" (the final consummatory phase of the prey-catching sequence) by means of stimuli applied to the optic tectum was analyzed using electromyographic methods in freely-moving Japanese toads. Electromyographic activities were recorded from the following 7 muscles in the head region (the presumed "snapping"-related muscles): M. depressor mandibulae, M. temporalis, M. sternohyoideus, M. geniohyoideus, M. genioglossus, M. hyoglossus, and M. submentalis. It was found that the characteristic activities evoked in these muscles were associated with jaw/tongue movements during the electrically-evoked "snapping". All of these muscles, except for sternohyoideus and geniohyoideus muscles, were activated in an all-or-nothing manner which corresponded to the elicitation of the electrically-evoked "snapping". It was suggested that such an all-or-nothing character may reflect an all-or-nothing property of the neuronal circuits for generating the prey-catching motor-pattern.

Animals↗
PubMed1985

Efferents from the supracommissural ventral telencephalon in the hime salmon (landlocked red salmon, Oncorhynchus nerka): an anterograde degeneration study.

Efferents from the area ventralis telencephali pars supracommissuralis (Vs) of hime salmon (landlocked red salmon, Oncorhynchus nerka) were studied using anterograde degeneration methods. It was found that Vs sends intratelencephalic fibers bilaterally to the internal cell layer of the olfactory bulb and wide areas of telencephalon, while it sends extratelencephalic fibers bilaterally to habenulae, inferior lobes and midbrain tegmenti through medial and lateral forebrain bundles and lateral preoptic area. A possibility was pointed out that some of these extratelencephalic projections are involved in the pathway controlling the sexual behavior which are suggested from behavioral experiments.

Animals↗
PubMed1985

Beneficial preparatory aortopulmonary shunt for anatomical repair of transposition of the great arteries with intact ventricular septum.

Anatomical repair was successfully performed in an infant with transposition of the great arteries and intact ventricular septum who had previously received a Blalock-Taussig shunt. In this patient, creation of an aortopulmonary anastomosis induced a well-developed left ventricle with concomitant adequately increased pressure and mass. Beneficial aortopulmonary shunt that precedes the anatomical repair in selected patients with simple transposition is discussed.

Aorta↗
PubMed1985

High incidence of aortic regurgitation following Kawasaki disease.

Among 131 patients with Kawasaki disease who underwent cardiac examinations from December 1979 to July 1984, six (4.6%) had aortic regurgitation documented on aortography. A protodiastolic murmur typical of aortic regurgitation was heard in four patients. Both cardiac enlargement on chest roentgenogram and ECG signs of left ventricular hypertrophy were demonstrated in only half of the patients. Of five patients who underwent a second angiographic study 1 year later, two no longer had aortic regurgitation; pulsed Doppled echocardiography at this point revealed aortic regurgitant flow in the remaining three patients. All patients had associated coronary artery aneurysms, and two patients developed subsequent myocardial infarction. The development of aortic regurgitation following Kawasaki disease has been considered to be causally related to the carditis that most frequently occurs during the acute stage of the illness. Our data indicate that aortic regurgitation is not rare but is an important additional cardiovascular complication of Kawasaki disease.

Aneurysm↗
PubMed1985

Sequential studies of rat urinary bladder epithelial lesions induced by ifosfamide (Z4942).

Urinary bladder damage caused by ifosfamide in male F344 rats was studied by light microscopy and scanning electron microscopy. Ifosfamide was injected intraperitoneally at doses of 30, 60 and 120 mg. per kg. body weight, and rats were killed at several intervals following treatment. The changes in the epithelium observed by light microscopy and scanning electron microscopy following ifosfamide injection were compared to those observed following cyclophosphamide injection. Necrosis and exfoliation of the urinary bladder epithelium occurred after day 1 of ifosfamide treatment and were followed by regenerative hyperplasia. This hyperplasia was reversible. A dose response was evident in the number and size of lesions induced and the time of regeneration and repair. Scanning electron microscopy disclosed short, uniform microvilli on the luminal surface of cells during the early phases of hyperplasia. These microvilli persisted for only 1 day, from days 1 to 7, and days 1 to 12 respectively, following injection of 30, 60 and 120 mg. per kg. of ifosfamide. The hyperplastic lesions also contained cells with pleomorphic microvilli and ropy or leafy microridges on their surfaces. These findings after ifosfamide administration were similar to those reported to be induced in the urinary bladder by cyclophosphamide.

Animals↗
PubMed1985

Clinicopathological study of the heart and coronary arteries of autopsied cases from the community of Hisayama during a 10-year period. Part V. Comparison of autopsy findings with electrocardiograms--Q.QS items of the Minnesota Code.

In a longitudinal study of a general population in Hisayama, Japan, 339 persons aged 40 years or over at death were autopsied during the period November 1, 1961-October 31, 1971. In 308 of these people, electrocardiograms taken at the periodic examinations were available, and Q.QS items of the Minnesota Code were recorded in 49 persons. The sensitivity of item 1-1 to autopsy-proven old myocardial infarction was 0.32 and the specificity was 0.95. Using the estimated prevalence of old myocardial infarction in this community--77 per 100,000--the proportion of persons without old myocardial infarction among those with item 1-1 in the general population (PF+) was 0.9954 and the proportion of persons with old myocardial infarction among those without item 1-1 (PF-) was 0.0005. The sensitivity of items 1-1 and 1-2 to old myocardial infarction was 0.43 and the specificity was 0.94. PF+ of items 1-1 and 1-2 was 0.9949 and PF- was 0.0005. These large values of PF+ mean that more than 99 per cent of persons with these items are probably false positives if these items are used as a screening test for this disease in the general population of this community, i.e., that these items cannot be defined as a definite myocardial infarction.

Aged↗
PubMed1985

Nasopharyngeal teratoma attached to the lower jaw.

A case of an intraoral teratoma attached to both the maxillary and mandibular regions is reported. This case strongly suggests the relationship between the buccopharyngeal membrane and intraoral teratoma. However, the number of cases showing such attachments is too small to permit a definite conclusion.

Female↗
PubMed1985

Cellular and humoral immune responses of mice subclinically infected with Pneumocystis carinii.

Cellular and humoral immune responses to Pneumocystis carinii were investigated. ICR and DDD mice were intranasally infected with 10(4) mouse lung-derived P. carinii, and the delayed-type hypersensitivity (DTH) reaction and antibody titers to P. carinii were measured along with the number of P. carinii cysts in the lungs after infection. The number of P. carinii cysts in the lungs peaked at 2 weeks after infection and then decreased to barely detectable levels by 4 weeks. Serum antibody (immunoglobulin G) titers measured by indirect immunofluorescence increased up to 4 weeks. The DTH footpad reaction was most prominent at 2 weeks postinfection and declined thereafter. Thus, the decline in the number of P. carinii cysts in the lungs corresponded well with the time of the peak of the DTH reaction but not with the serum antibody response. Spleen T cells from infected mice mediated the DTH reaction when transferred intravenously into normal recipients and reduced the number of P. carinii cysts in the lungs when transferred intravenously into P. carinii-infected mice. The results indicated that cellular immunity is important for protection from subclinical P. carinii infections.

Animals↗
PubMed1985

Serological observations of Pneumocystis carinii infection in humans.

Serological examinations of Pneumocystis carinii antigen and antibody were performed in adult patients with P. carinii pneumonia. The antibody titer was assayed by indirect immunofluorescence, and antigenemia was detected by counterimmunoelectrophoresis. Profiles of the time course of antibody levels in serum indicated that development of the antibody occurred at an early stage of pneumonia and that peak titers were obtained during the febrile stage.

Adult↗
PubMed1985

ADP-ribosylation.

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Adenosine Diphosphate Ribose↗