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Biomedical subjects

K Ueda

Publications and source records attributed to K Ueda.

At least 1,279 records · Page 71Linked to original sources
PubMed1986

[Histopathological changes in endometrial cancer treated with tamoxifen].

Eight cases of endometrial adenocarcinoma, including 7 well differentiated cases (stage Ia three, stage Ib three, stage II one) and I poorly differentiated case (stage IVb), were treated with Tamoxifen (20 mg/day) for 7-14 days. The average age of these patients was 59.1 +/- 10.9 years old and they were all postmenopausal for between 6 months and 30 years. The endometrial tissue was biopsied before Tamoxifen administration and half of it was investigated for progestin receptor (PR) and estrogen receptor (ER) while the remainder was used for histopathological examination. After surgical treatment, some tissue fragments of the endometrium were taken for investigation of PR and ER. The effect of Tamoxifen was then studied for the amount of PR, ER and histopathology. The result was that the amount of PR was increased in well differentiated cases whereas it was unchanged in poorly differentiated ones, and the amount of ER was decreased in all cases. Meanwhile, obvious histopathological changes were noted as follows decrease of glandular epithelial stratification, increased secretory activity, increased epithelial cell size with columnar cell type, increase of transparent, vacuolated cytoplasm, increase of nuclear size with variable shape and prominent nucleoli with marginal aggregation of chromatin. However, no stromal change was noted. Furthermore, the greater the increase in the amount of PR, the more conspicuous histopathological change was noted. It was concluded that Tamoxifen could produce both functional (PR, ER) and morphological (histopathology) changes in endometrial cancers.

Adenocarcinoma↗
PubMed1985

An HRP study of afferent connections of the supracommissural ventral telencephalon and the medial preoptic area in himé salmon (landlocked red salmon, Oncorhynchus nerka).

The supracommissural ventral telencephalon and the medial preoptic area have been shown to play important roles in the sexual behavior of himé salmon (landlocked red salmon, Oncorhynchus nerka). In the present study, the sites of neurons projecting to these regions were examined by means of the retrograde horseradish peroxidase (HRP) tracing method. The morphology of neurons in these sites of origin was also studied by means of the Golgi method. The nucleus preopticus periventricularis and the rostral part of nucleus preopticus (NPP-rNPO) received bilateral projections from the middle parts of the area ventralis telencephali pars ventralis (Vv) and the area ventralis telencephali pars dorsalis (Vd), NPP and lateral part of the preoptic area (LPOA), ipsilateral projections from the caudal part of Vv, nucleus anterioris periventricularis (NAPv), nucleus ventromedialis thalami (NVM) at the level of the posterior commissure, nucleus lateralis tuberis pars medialis (NLTm), nucleus anterior tuberis (NAT), nucleus saccus vasculosus (NSV), nucleus recessus posterioris (NRP) and midbrain tegmentum (TG), and a projection from the nucleus posterior tuberis (NPT), which is situated on the midline of the brain. The area ventralis telencephali pars supracommissuralis and neighboring caudal ventral telencephalon (Vs-cV) received ipsilateral projections from almost all parts of the Vv, the middle and caudal parts of Vd, almost all parts of the NNP, the NPO at the level between the habenula and the posterior commissure, and the rostral part of the nucleus dorsomedialis thalami (NDM). The Vs-cV also received a projection from NPT. These findings seem to give anatomical bases for understanding the neural mechanisms involved in sexual behavior as well as neuroendocrine functions.

Afferent Pathways↗
PubMed1985

Repeated quantitative angiograms in coronary arterial aneurysm in Kawasaki disease.

Accurate evaluation of the extent of coronary artery lesions complicating Kawasaki disease is clinically important in patient management. Based on a total of 188 coronary angiograms and retrospective follow-up observations, the condition of coronary aneurysms was quantitatively graded as: 0 (normal)--no significant enlargement in any portion of the coronary artery; I (mild)--aneurysmal dilatation of the coronary artery evident but localized, with a maximal diameter of less than 4.0 mm; II (moderate)--maximal diameter of coronary aneurysms between 4.0 and 8.0 mm, regardless of body size; III (severe)--giant aneurysms, with the maximal diameter greater than 8.0 mm. Most mild coronary aneurysms regressed to normal within a short time, and the patient's prognosis was good. The course of grade II aneurysms varied, depending on initial angiographic coronary diameter, but all were eventually reduced in coronary size. In contrast, grade III aneurysms usually progressed to become obstructive or stenotic coronary lesions, or the large aneurysm persisted. Follow-up observations revealed that the course of coronary artery disease depended on the size and distribution of aneurysms at initial angiography. This grading of the severity of coronary lesions may provide useful criteria for predicting the prognosis of patients with Kawasaki disease.

Aneurysm↗
PubMed1985

Acute hemodynamic effects of nitroprusside in children with isolated mitral regurgitation.

The acute hemodynamic responses to nitroprusside during cardiac catheterization were evaluated in 12 children with isolated mitral regurgitation (MR) of congenital origin. The patients were classified into 2 groups. Group I consisted of 5 patients who received digitalis or diuretic drugs and group II consisted of 7 patients who received no medication. Hemodynamic measurements were performed using a multisensor catheter system during infusion of nitroprusside at a dose of 2.0 micrograms/kg/min. With nitroprusside, the mean right atrial pressure decreased an average of 50% in group I (from 6 +/- 1 to 3 +/- 1 mm Hg) and 75% in group II (from 4 +/- 1 to 1 +/- 1 mm Hg), and the mean decrease of pulmonary artery wedge pressure was 50% in group I (from 14 +/- 3 to 7 +/- 3 mm Hg) and 56% in group II (from 9 +/- 2 to 4 +/- 1 mm Hg). Administration of nitroprusside also caused a significant reduction in systemic vascular resistance by 40% in both groups (from 25 +/- 6 to 15 +/- 5 U/m2 in group I and from 25 +/- 2 to 15 +/- 3 3 U/m2 in group II), whereas forward cardiac output increased markedly, by an average of 48% in group I (from 3.3 +/- 0.5 to 4.9 +/- 0.9 liters/min/m2) and 58% in group II (from 3.3 +/- 0.3 to 5.2 +/- 0.8 liters/min/m2). No significant differences in hemodynamic response between groups I and II were noted.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Flow Velocity↗
PubMed1985

Coronary atherosclerosis and antecedent risk factors: pathologic and epidemiologic study in Hisayama, Japan.

The pathologic and epidemiologic study of coronary atherosclerosis was performed on 281 autopsy subjects aged 40 years and older during the period between 1971 and 1981, in Hisayama, Kyushu Island, Japan. During this interval, the autopsy rate was about 80%. Grading of coronary atherosclerosis was made by the degree of luminal stenosis. Seven selected factors such as age, gender, mean arterial blood pressure, serum total cholesterol, body mass index, alcohol intake and cigarette smoking were all given attention. The severity of coronary atherosclerosis revealed age-related progression. Coronary atherosclerosis correlated positively with antecedent elevated blood pressure and with higher serum total cholesterol levels. In multivariate analysis, factors that significantly (p less than 0.05) and positively correlated with coronary atherosclerosis were age, mean arterial blood pressure, serum total cholesterol, gender and body mass index in this order. The overall contribution of 7 selected factors to coronary atherosclerosis was nearly 33.5% of the total variation by the analysis of multiple regression model.

Adult↗
PubMed1985

Ganglion cells of the terminal nerve: morphology and electrophysiology.

The ganglion cells of the terminal nerve (TN cells) in the carp were identified using intracellular recording and staining techniques. The TN cells showed characteristic location and morphology as well as characteristic responses after electrical shocks to the olfactory nerve and tracts. These features of the TN cells were distinct from those of the mitral or other cells in the olfactory bulb.

Animals↗
PubMed1985

Trapping of reactive intermediates in enzymology. Exogenous flavin reduction during catalytic turnover of substrate by glyoxalase I.

Although but weak inhibitors of glyoxalase I under steady-state conditions, flavins are reduced by yeast glyoxalase I (lactoyl-glutathione lyase, EC 4.4.1.5) plus its substrate (the hemithiolacetal from glutathione and phenylglyoxal) during catalytic turnover. Studies with 10-ethylisoalloxazine showed that this flavin reduction was peculiar not merely to glyoxalase I's substrate, but was characteristic of the complete system, enzyme plus substrate undergoing catalytic turnover. Flavins are poor hydride-ion acceptors and the reduction observed most likely represents an oxidative trap of a transient carbanion formed in the glyoxalase I mechanism of action. Hydrophobic flavins were more efficient traps than the hydrophilic ones, and values of the Km for the phenylglyoxal: glutathione hemithiolacetal adduct measured by the flavin-reaction and by normal steady-state kinetics were closely similar. This argues that trapping has occurred of an enediolate ion (an enzyme-generated carbanion) still bound to glyoxalase I.

Animals↗
PubMed1985

Cleavage of stem-and-loop structure DNA by bleomycin. Reaction on the bacteriophage G4 origin of complementary strand synthesis.

The cleavage by bleomycin-Fe(II) complex in the presence of dithiothreitol of 3'-or 5'-end-labeled DNA from the region of the bacteriophage G4 origin of complementary strand synthesis was investigated by using the DNA-sequencing technique. Bleomycin cleaved a single-stranded DNA substrate preferentially at inverted repeat sequences, which potentially form stem-and-loop structures, while it cleaved double-stranded DNA substrates with different specificity. The results support the formation of three adjoining stem-and-loop structures in the region of the phage G4 origin of complementary strand synthesis under the low-salt conditions used and suggest a difference in the form of the double helix between the stem and the double-stranded DNA fragment. Bleomycin appears to be a useful reagent for searching stem-and-loop structures. The results may also contribute to the understanding of the mode of action of bleomycin as an antitumor antibiotic.

Bacteriophages↗
PubMed1985

Site-specific DNA damage caused by lipid peroxidation products.

DNA damage induced by autoxidized lipids was investigated using covalently closed circular (supercoiled) DNA and DNA fragments of defined sequence. DNA-strand-breaking substances accumulated during autoxidation of methyl linolenate, and strand breakage was measured with samples taken at different times. The DNA-strand-breaking activity reached its maximum a little after the peak value of peroxide and decreased upon further autoxidation. The peak of the DNA-strand-breaking activity did not always coincide with the peak of thiobarbituric acid reactants or of conjugated diene, either. The DNA-strand-breaking reaction was dependent on metal ions and was inhibited by potassium iodide and tiron and partially by catalase, suggesting the involvement of radical species and/or oxygen radicals. No direct cleavage of singly end-labeled 100-200 basepair DNA fragments by autoxidized methyl linolenate and cupric ion was detected under the conditions used. Cleavage occurred during subsequent heating in piperidine after the reaction. The alkali-labile damage was preferentially induced at pyrimidine residues, especially in dinucleotide sequences of pyrimidine-guanine (5'----3'), which was determined by sequencing.

1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium ↗
PubMed1985

Sequence specific damage of DNA induced by reducing sugars.

Reducing sugars induced alkali-labile sites in DNA. The DNA reacted with D-fructose 6-phosphate or D-fructose in the presence of Cu2+ was cleaved by the treatment with aqueous piperidine at 90 degrees C for 30 min. Alkali-labile sites were induced frequently at the pyrimidine residues, especially at the pyrimidine residues in pyrimidine-purine (5'----3') sequences. Transition metal ions such as Cu2+ and oxygen radicals such as hydrogen peroxide were possibly involved in the induction of alkali-labile sites.

Base Sequence↗
PubMed1985

Tongue-muscle-controlling motoneurons in the Japanese toad: topography, morphology and neuronal pathways from the 'snapping-evoking area' in the optic tectum.

As a step to clarifying the neural bases for the visually-guided prey-catching behavior in the toad, special attention was paid to the flipping movement of the tongue. Tongue-muscle-controlling motoneurons were identified antidromically, and their topographical distribution within the hypoglossal nucleus, the morphology, and the neuronal pathways from the optic tectum including the 'snapping-evoking area' (see below) to these motoneurons were investigated in paralyzed Japanese toads using intracellular recording techniques. The morphology of motoneurons innervating the tongue-protracting or retracting muscles (PMNs or RMNs respectively) was examined by means of intracellular-staining (using HRP/cobaltic lysine) and retrograde-labeling (using cobaltic lysine) methods. Both PMNs and RMNs showed an extensive spread of the branching trees of dendrites; 4 dendritic fields were distinguished: lateral/ventrolateral, dorsal/dorsolateral, medial, and in some motoneurons, contralateral dendritic fields, although there was a tendency for the dorsal/dorsolateral dendritic field to be less extensive in the PMNs than in the RMNs. The axons of both PMNs and RMNs arose from thick dendrites, ran in a ventral direction without any axon-collaterals branching off, and then entered the hypoglossal nerve. The PMNs and RMNs were distributed topographically within the hypoglossal nucleus; the RMNs were located rostrally within the nucleus, whereas the PMNs were located more caudally within it. In about 3/4 of the RMNs tested, depolarizing potentials [presumably the excitatory postsynaptic potentials (EPSPs)], on which action potentials were often superimposed, were evoked by electrical stimuli applied to the nerve branch innervating the tongue protractor. These EPSPs were temporally facilitated when the electrical stimuli were applied at short intervals (10 ms). Both PMNs and RMNs showed hyperpolarizing potentials (IPSPs) in response to single electrical stimuli of various intensities (10-200 microA) applied to the 'snapping-evoking area' (lateral/ventrolateral part of the optic tectum) on either side. These IPSPs were facilitated after repetitive electrical stimulations at short intervals (10 ms) and of weaker intensities (down to 10 microA); i.e., a temporal facilitation of the IPSPs was observed. On the other hand, large and long-lasting EPSPs which prevailed over the underlying IPSPs were evoked after repetitive electrical stimulations (a few pulses or more) at short intervals (10 ms) and of stronger intensities (generally 90 microA or more); thus, a temporal facilitation of the EPSPs was also observed.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗