Biomedical subjects

K Tamura

Publications and source records attributed to K Tamura.

At least 1,225 records · Page 68Linked to original sources

PubMed1984

Immunochemical identification of an alpha 1-acid glycoprotein-antigenic determinant on carcinoembryonic antigen (CEA) and non-specific cross-reacting antigen (NCA).

An immunochemical characterization of carcinoembryonic antigen (CEA) and NCA (non-specific cross-reacting antigen) was performed. Positive reactions of CEA and NCA (Mr 60 000) with some antibodies to alpha 1-acid glycoprotein (AG) were observed. Thus, both antigens may contain immune determinants in common with alpha 1-acid glycoprotein. CEA showed positive reactivity with anti-NCA. NCA showed positive reactivity with either polyclonal or monoclonal antibodies to CEA, but negative reactivity with auto-antibodies to CEA. 125I-Tetracosapeptide (synthetic peptide-24 corresponding to the amino terminal sequence of CEA) failed to react with any antisera against CEA, NCA and AG. 125I-AG also showed no immuno-reaction with any antibody against CEA, NCA and tetracosapeptide. These results suggest that some monoclonal antibodies to CEA are directed against a common antigenic determinant of both CEA and NCA in addition to AG and tetracosapeptide, and that the auto-antibody to CEA is directed against a unique immune determinant which is not common to NCA. Thus, CEA appears to contain a unique determinant not found in NCA. Similarities in the composition of both amino acids and carbohydrates of CEA and NCA suggest that CEA is 'big-big' AG and NCA is 'big' AG.

Antibodies, Monoclonal↗

PubMed1984

Increased frequency of isolation of Pasteurella and Actinobacillus species and related organisms.

Ninety-six clinical isolates of Pasteurella, Actinobacillus and related organisms were submitted to our reference laboratory for identification. The procedures for detecting the 11 identified species, Pasteurella multocida, Pasteurella haemolytica, Pasteurella ureae, Pasteurella pneumotropica, Actinobacillus lignieresii, Actinobacillus equuli, Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Cardiobacterium hominis, and two unnamed species of CDC groups, HB-5 and EF-4, are described and their clinical importance is discussed. These organisms have been increasingly isolated in Japan and are most often associated with respiratory infections and endocarditis.

Actinobacillus↗

PubMed1984

Multiple regression analysis of the cholinesterase activity with certain physiochemical factors.

Nineteen hundred sixteen agricultural workers who have been living in a rural area in central Japan were studied. Their age, blood pressure, Broca index, hemoglobin content, serum total cholesterol, and activity of serum cholinesterase and transaminase (GOT and GPT) were determined. The relation between the cholinesterase activity and certain physiochemical factors of the subjects was studied. Cholinesterase activity (ChE) is related to certain factors such as age, hemoglobin content (Hb), serum total cholesterol (TCh), transaminase activity (GPT), and Broca index (BI). A multiple regression equation exists between the cholinesterase activity and these factors: ln ChE = a (age) + b (Hb2) + c X ln GPT + d X ln TCh + e BI + f, where a, b, c, d, e, and f are constants. The estimated value of cholinesterase activity agrees with its measured activity. The partial correlation coefficients of the equation can be classified into the following three classes: (1) The partial correlation coefficients of total cholesterol and Broca index are nearly constant without distinction of sex and season. (2) The coefficient of hemoglobin content has a small seasonal and sexual difference. (3) The coefficients of age and GPT have a great seasonal and sexual difference. Using the equation, the most probable value of cholinesterase activity can be estimated. Therefore, the significant changes of its activity may be attributed to the toxic effects of insecticides or the abnormality of liver function.

Adult↗

PubMed1984

Clinical significance of autorosette-forming cells in T-cell lymphoma.

The capability to form rosettes with sheep erythrocytes (E), antibody-complement-sensitized ox erythrocytes (EAC) and autologous erythrocytes (ARFC), and surface immunoglobulin determinations were studied using 21 lymph nodes and one tonsil with pathologically-proven non-Hodgkin's lymphoma and 10 lymph nodes with benign pathology. Fourteen of 22 non-Hodgkin's lymphoma patients (64%) had a high incidence of E-rosette formation and they were further differentiated into ARFC-positive and ARFC-negative lymphomas. The clinicopathological findings of the latter were compatible with those of adult T-cell leukemia. ARFC-positive lymphoma was regarded as non-Hodgkin's lymphoma of T-cell type and one patient showed lymphoblastic lymphoma with high ARFC counts. ARFC counts were very low in B-cell and non-T, non-B lymphomas. The results from benign lymph nodes were too variable to draw any conclusion, although ARFC counts were relatively high in lymphadenitis and hyperplasia.

Humans↗

PubMed1984

Effects of pressure on the phase transition of bilayers in liposomes. Influence of cholesterol and alpha-tocopherol.

The effects of pressure on the gel-to-liquid crystalline phase transition temperature of dimyristoylphosphatidylcholine (DMPC) bilayers containing cholesterol, alpha-tocopherol, and alpha-tocopheryl acetate were studied by fluorescence depolarization. The transition temperature of cholesterol mixtures (greater than 7.5 mol%) was lower than that of 100% DMPC at atmospheric pressure, but it became higher than the latter on increase in pressure. The thermodynamic parameters of the transition (delta V, delta S, delta H) were estimated and the functions of cholesterol and alpha-tocopherols in the bilayers are discussed.

Cholesterol↗

PubMed1984

Cultured human monocytes, granulocytes and a monoblastoid cell line (THP-1) synthesize and secrete immunosuppressive acidic protein (a type of alpha 1-acid glycoprotein).

Immunosuppressive acidic protein (IAP, pI 3.0) is a type of alpha 1-acid glycoprotein (alpha 1-AG). The secretion of IAP into the culture fluids of different subpopulations of human peripheral blood leukocytes was examined by a newly devised passive hemagglutination (PHA)-inhibition test. Human peripheral monocytes, an established monoblastoid cell line (THP-1) and peripheral granulocytes produced IAP. However, neither T nor B lymphocytes, nor lymphoblastoid cell lines induced by TCGF or EB virus respectively, produced IAP. The IAP concentration reached a maximum (215 ng/ml) in the culture fluids of peripheral monocytes (1 X 10(6)/ml) when monocytes were stimulated by the addition of either immune complex, carrageenan or endotoxin. The synthesis de novo and shedding of IAP by THP-1 were demonstrated by the immunoprecipitation of radioactive IAP in the culture fluids of [3H]leucine-labeled cells. SDS-polyacrylamide gel electrophoresis of the immunoprecipitates showed two peaks of radioactivity, one comigrated with authentic IAP at 50,000 daltons, and the other at 38,000 daltons, suggesting that two different forms of IAP (and/or alpha 1-AG) are produced from human monocytes.

Animals↗

PubMed1984

Increase in autorosette-forming lymphocytes in thyroid diseases.

A subpopulation of lymphocytes forming rosettes with autologous erythrocytes was studied on peripheral blood and thyroid tissues obtained from the patients with various thyroid diseases. The mean (+/-S.D.) percentage of autorosette-forming cells (ARFC) was 10.1(+/-5.5)% in the peripheral blood from patients with hyperthyroid Graves' disease, which was higher than that in normal subjects (5.6 +/- 2.8%), while the levels of ARFC in the peripheral blood from euthyroid patients with Graves' disease under treatment and Hashimoto's thyroiditis did not significantly differ from the normal level. The mean percentages of ARFC in the thyroid tissues from patients with Graves' disease and Hashimoto's thyroiditis were 14.7(+/-8.5) and 13.3(+/-7.8)%, respectively, which were higher than those in the peripheral blood from the same patients. Most of these cases with abnormally high levels of ARFC were accompanied with the abnormally low T cell to B cell ratios. The microscopic examination of the cytological materials from these patients showed an increased number of large stimulated lymphoid cells or lymphoblasts as compared with those who had few ARFC. These results suggest an increase in an activated T cell subset in the circulation and/or in the thyroid tissue, which is probably caused by active immune response to some stimuli.

Adolescent↗

PubMed1984

Sequence of occurrence of abnormal regional cardiac function, global pump function and electrocardiogram after brief ligation and reperfusion of a coronary artery.

The following results were obtained. During brief ligation of the coronary artery, a change in the cardiac wall motion appeared as the first abnormality of the CMG. Succeedingly, epicardial ECG changes were recognized, and lastly, changes of dP/dt appeared. After reperfusion of the coronary artery, dP/dt was quickly normalized, followed by restoration of ST elevation. Cardiac motion, measured by CMG, was the last abnormality to improve.

Animals↗

PubMed1984

Serum somatomedin A in Perthes' disease.

Serum somatomedin A, determined by radioreceptor assay in 47 children with Perthes' disease, was significantly reduced as compared to normal children of the same age. Nine children underwent the 1-Dopa tolerance tests to evoke growth hormone release; a normal response was found in eight. Patients with Perthes' disease tend to be short in stature with "disproportionate skeletal growth" of the limbs; growth is less in the distal than in the proximal extremities. The low serum levels of somatomedin A suggest some growth disturbance during the development of Perthes' disease.

Adolescent↗

PubMed1984

[Treatment for advanced malignant lymphoma in patients with compromised bone marrow--a combination therapy using pepleomycin, vincristine and high-dose adrenocorticoids (POP regimen)].

It is often very frustrating for clinicians when in cases where Hodgkin's disease or non-Hodgkin's lymphoma is relapsing rapidly, sufficient doses of cytotoxic agents and/or radiation therapy cannot be given because of cytopenia due either to previous treatment or to bone marrow infiltration by the lymphoma. We have been treating such patients with a combination chemotherapy consisting of vincristine 0.25 mg/day i.v. push daily for 4 days, pepleomycin 5 mg/day s.c. by continuous infusion daily for 4 days, and prednisolone 1 g/day p.o. or methylprednisolone 1 g/day d.i.v. every other day for 4 days POP combination) administered every 2 to 3 weeks until an improvement in cytopenia occurs. Twelve patients with advanced non-Hodgkin's lymphoma, including 8 T-cell lymphoma patients, entered this pilot study when they were cytopenic or had been treated with cytotoxic agents or radiation therapy immediately prior to POP therapy. All patients responded and experienced more than 50% tumor reduction associated with improved general condition. Untoward effects included transient glycosuria, mild decline of serum immunoglobulin levels, herpes zoster and temporary aggravation of cutaneous or oral fungal infection in each of one to two patients. Since this treatment is switched to other forms of treatment when bone marrow function has recovered, duration of the response cannot be determined. The survival time was rather short because of poor general condition and aggravation of concurrent active pulmonary infection before the start of treatment. This treatment is sufficient effective, however, to be tried on patients with active lymphoma, not necessarily associated with poor bone marrow function, unless active infection supervention.

Adult↗

PubMed1984

[Induction of tumor necrosis factor (TNF) in tumor-bearing mice].

Tumor necrosis factor (TNF) can be induced in BALB/c mice bearing syngeneic tumor RL male 1 lymphoma or Meth A fibrosarcoma, by initial injection of either Corynebacterium parvum or BCG, in combination with a second injection of endotoxin (LPS) almost 10 days later. Either treatment resulted in hemorrhagic necrosis and complete regression of tumors in mice. The term, "endogenous induction of TNF therapy" was proposed for this trial. Although the TNF of tumor-bearing mice was identical to that of normal mice in molecular weight (about 40,000 daltons) and isoelectric point (4.4), and differed from IFN, the in vivo effect of endogenous TNF was much more remarkable than in mice receiving passive TNF administration. The mice bearing RL male 1 lymphoma cured after endogenous induction of TNF, rejected rechallenge of the same tumor cells but not Meth-A tumor cells. Splenocytes obtained from these cured mice were also found to be inhibitory in Winn's neutralization assay. The data indicate that tumor-specific immunity can be induced in mice receiving endogenous induction of TNF.

Animals↗

PubMed1984