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Biomedical subjects

K Shimada

Publications and source records attributed to K Shimada.

At least 1,423 records · Page 79Linked to original sources
PubMed1984

Phorbol ester increases the level of interleukin 2 mRNA in mitogen-stimulated human lymphocytes.

TPA alone did not induce the production of IL 2 in human tonsillar lymphocytes but enhanced the PHA-induced IL 2 production by seven-fold. That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. In these ways, it was shown that TPA alone did not induce any significant IL 2 mRNA synthesis, but when added together with PHA it increased the level of IL 2 mRNA by at least 10-fold, as compared with that induced by PHA alone.

Animals↗
PubMed1983

Isolation and characterization of a human interleukin 2 gene.

An interleukin 2 (IL-2) gene was isolated from a Charon 4A human gene library. Electron microscopic examination of 15 heteroduplexes formed between the genomic DNAs and the IL-2 cDNAs demonstrated that the size of the IL-2 gene is about 5.1 +/- 0.5 kb and that there are at least two introns in this gene. Nucleotide sequence of the 5' flanking region of the IL-2 gene showed a homology with that of the corresponding region of the human immune interferon gene.

Base Sequence↗
PubMed1983

Cloning of interleukin 2 mRNAs from human tonsils.

Human interleukin 2 (IL-2) mRNAs were cloned from a cDNA library prepared from mitogen-stimulated tonsillar mononuclear cells. One of these clones was sequenced and, using this cDNA as a hybridization probe, Southern blot analysis of the human placental DNA was performed. Our results indicate that there is only one IL-2 gene in the human genome.

Cloning, Molecular↗
PubMed1983

The majority of cDNA clones with strong positive signals for the interferon-induction-specific sequences resemble mitochondrial ribosomal RNA genes.

A complementary DNA library prepared from the 12S polyadenylated RNAs extracted from interferon-induced KG-1 cells, a human myeloblast cell line, was screened for the presence of induction-specific sequences. Clones that exhibited strong positive signals were separated by hybridization criteria into nine classes. Clones from classes I through IV consisted of about 78% of the total and unexpectedly were found to resemble human mitochondrial ribosomal RNA genes.

Base Sequence↗
PubMed1983

High-performance liquid chromatographic determination of phenytoin and hydroxyphenytoin in human urine.

Simple and rapid quantitative analysis utilizing high-performance liquid chromatography was performed to determine the concentration of the antiepileptic agent phenytoin, and its main metabolite hydroxyphenytoin, in human urine. For the purposes of simple and rapid determination, the commercially available Extrelut columns were used with diethyl ether and chloroform as extraction solvents. High-performance liquid chromatography was performed on a LiChrosorb RP-8 column, with a mobile phase of methanol-0.02% ammonium acetate (1:1). The internal standard was 5-(4-methylphenyl)-5-phenylhydantoin. The method was applied to patients' urine, to examine the influence of concomitant drugs. Also, the results obtained using the commercially available enzymatic immunoassay method were compared with those from the present method, and it was concluded that a simple and rapid microanalysis is possible with a high extraction ratio.

Adult↗
PubMed1983

High-performance liquid chromatographic determination of free fatty acids with 1-naphthylamine.

A 1% benzene solution of oxalyl chloride was added to saturated and unsaturated fatty acids and the mixture was allowed to react at 70 degrees C for 30 min; by this procedure, each fatty acid was converted into its acid chloride in a considerably quantitative manner. By reacting this acid chloride with 1-naphthylamine at 30 degrees C for 15 min, naphtylamine derivatives were produced, which showed strong ultraviolet absorption around 280-290 nm. Experiments were made on the recovery of the fatty acids added to 0.5 ml of human serum, and the recovery was found to fall in the range of 94-106% (coefficient of variation = 0.5-4.1%) when the following amounts of six fatty acids were added: C14:0, 2 micrograms; C16:0, 20 micrograms; C16:1, 5 micrograms; C18:0, 4 micrograms; C18:1, 20 micrograms; C18:2, 10 micrograms.

1-Naphthylamine↗
PubMed1983

Presence of mitochondrial-DNA-like sequences in the human nuclear DNA.

Two lambda phage clones carrying contiguous human nuclear DNA sequences with extensive homology to non-contiguous human mitochondrial 16S ribosomal RNA sequences were isolated from a human gene library. The one clone carried mitochondrial-DNA(mtDNA)-like sequences flanked with two kinds of repetitive nuclear DNA sequences and the other carried mtDNA-like sequences, between unique nuclear DNA sequences and repetitive DNA sequences of Alu-family. These results demonstrate that mtDNA-like sequences are present in human nuclear DNA.

Base Sequence↗
PubMed1983

Clinical evaluation of ceftazidime in internal medicine in Japan.

A total of 424 medical patients were treated with ceftazidime: 376 were assessable for clinical efficacy and 413 for possible adverse events. The overall clinical efficacy rate was 80%. The eradication rates of initial pathogens were 82% in monomicrobial infections and 71% in polymicrobial infections. Adverse events considered to be attributable to ceftazidime were observed in 15 (3.6%) of the patients. Drug-related changes in laboratory parameters occurred in 29 patients but were always mild and transient. Ceftazidime appears to be a safe, efficacious antibiotic for treatment of infections in internal medicine.

Adult↗
PubMed1983

Construction of a lambda packageable ColE1 vector which permits cloning of large DNA fragments: cloning of thyA gene of Escherichia coli.

A small packageable plasmid depending on the ColE1 replicon was constructed from ColE1-gal-cos lambda:: Tn3, pKY2113 (T. Nakamura et al. (1981) J. Biochem. 90, 1013), and named pKY2662. This plasmid carries ampicillin resistance and colicin E1 immunity genes as selective markers, and has neither mobilization function nor movable transposon. The molecular size of pKY2662 is 8.7 kb, and it has a single cleavage site each for BamHI, Bg/II, ClaI, EcoRI, HpaI, PstI, and Tth111I. By using pKY2662 as a vector, a 32 kb Escherichia coli DNA fragment covering thyA, recC, recB, and argA genes was cloned. This new small cosmid is among the most efficient vectors hitherto found for in vitro cloning of large DNA fragments.

Bacteriophage lambda↗
PubMed1983

Monoclonal antibody against microtubule associated protein-1 produces immunofluorescent spots in the nucleus and centrosome of cultured mammalian cells.

A monoclonal antibody was raised against the highest molecular weight protein associated with microtubules (MAP-1). Its specific binding to MAP-1 was determined by immunoblotting of the gel electrophoretogram of microtubule proteins prepared from porcine brain. The antibody reacted only with MAP-1, not with MAP-2, tau or tubulin. Indirect immunofluorescent staining by this antibody showed bright intranuclear spots, the centrosome and the faint meshwork of the cytoplasm in several types of cultured mammalian cells; HeLa, PtK2, human skin fibroblasts, mouse melanoma cells, Chinese hamster ovary cells. The nuclear spots in the interphase cells, were replaced by diffuse enhanced fluorescence throughout the cell except for chromosomes during mitosis. They reappeared in late telophase, first in the cytoplasm, late in the nucleus. The punctate pattern of nuclear immunofluorescence was not affected by microtubule-depolymerizing agents. The result that it persisted on residual cell structures after extraction with a high salt concentration buffer containing Triton X-100 followed by digestion with DNase I and RNase A suggests that the antigen is associated with the nuclear skeleton.

Animals↗
PubMed1983

Antigenic properties of estriol 3-glucuronide-[C-6]-bovine serum albumin conjugates having oxime bridges.

The preparation and antigenic properties of estriol 3-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-3-carboxypropylcarbamoylmethyl)oxime bridge at the C-6 position on the steroid nucleus, has been described. 16,17-Di-O-acetyl-6-oxoestriol 6-(O-carboxymethyl)oxime 3-glucuronide acetate-methyl ester was condensed with gamma-amino-n-butyric acid by the mixed anhydride method. Subsequent coupling with BSA followed by removal of the protecting groups with alkali gave the desired hapten-BSA conjugate. The antisera elicited in the rabbit with the conjugate were highly specific to estriol 3-glucuronide, discriminating from other 3-substituted compounds. The specificity of antisera improved by elongation of the 6-(O-carboxymethyl)oxime bridge has been discussed.

Animals↗
PubMed1983

Further studies on the effects of flavine adenine dinucleotide on the platelet functions.

Flavine adenine dinucleotide (FAD) may inhibit not only the aggregation but also ATP release of platelets in vitro. FAD did not inhibit the synthesis of thromboxane from 1-14C-arachidonic acid by platelets. FAD was found to be hydrolyzed to FMN and AMP by a plasma enzyme, the activity of which in normal adults being estimated as 32.6 +/- 9.1 m microM/ml plasma/min and AMP, thus produced, and/or FAD might compete for the receptor sites on the platelet surface. This could be the mechanism of the inhibitory effect of FAD upon the platelet functions. The intravenous drip infusion of FAD in a dose of 0.2 mg/kg within 30 min was followed by no marked decrease of platelet aggregation, probably because the plasma concentration of FAD and/or AMP did not reach the levels high enough to suppress the platelet functions.

Adenosine Triphosphate↗