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Biomedical subjects

K Seki

Publications and source records attributed to K Seki.

At least 487 records · Page 27Linked to original sources
PubMed1981

A case of chronic veno-occlusive disease of the liver.

A case of chronic veno-occlusive disease of the liver manifested by ascites in a 59-year-old Japanese male is described. The patient had not been exposed to pyrrolizidine alkaloids, but had been receiving allopurinol for hyperuricemia, the only drug taken throughout the period in which the hepatic lesion developed. The hepatic veins and inferior vena cava were patent according to angiography. The first biopsy showed histological characteristics of the disease and the following two biopsies demonstrated the progression of the hepatic lesion from central fibrosis to non-portal cirrhosis. Morphometric analysis of the sublobular and central veins in the three serial biopsy specimens demonstrated stepwise decrease of the ratio of the veins per portal triad and stepwise increase of severely obliterative veins.

Allopurinol↗
PubMed1981

Elevated serum DHEA-S levels in association with hyperprolactinemia.

Serum DHEA-S levels were significantly higher in women with hyperprolactinemia than in normal women during the early follicular phase. When comparison was made of serum DHEA-S levels in hyper-and normoprolactinemic patients with secondary amenorrhea due to hypothalamic-pituitary failure, serum DHEA-S levels were significantly higher in hyperprolactinemic patients than in normoprolactinemic patients. This indicates elevated serum DHEA-S levels in association with hyperprolactinemia, but not with amenorrhea pe se.

Amenorrhea↗
PubMed1981

Changes of serum concentrations of dehydroepiandrosterone, its sulfate, 11-deoxy-17-ketosteroids, cortisol, estradiol, testosterone, prolactin and gonadotropins in normal prepubertal and pubertal females.

The concentrations of serum dehydroepiandrosterone (DHA), DHA sulfate (DHA-S), 11-deoxy-17-ketosteroids (11-deoxy-17-KS), cortisol (F), estradiol (E2), testosterone (T), prolactin (PRL), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured by means of radioimmunoassays in 157 prepubertal and pubertal girls whose age ranged from 5 to 16. Among the hormones studied, DHA exhibited a first significant increment at age 8, ahead of all other hormones. The significant increases of 11-deoxy-17-KS and DHA-S occurred at age 9 and 10, respectively. After the occurrence of the significant increment, the concentrations of these androgens increased progressively with age. DHA/DHA-S ratio showed a decreasing trend with the advance of age. F did not show any significant variation with age. E2 increased significantly at age 11 and T at age 10. PRL increased significantly at age 12 but its concentration did not seem to vary very much with age after 9. FSH and LH started to increase after age 9, with their significant increases occurring at age 10 and 9, respectively. These results confirm that during the course of pubertal development in girls, adrenal androgens increase first, which is followed by pituitary gonadotropins and then gonadal estrogen.

17-Ketosteroids↗
PubMed1981

Ultrastructure and some biologic properties of human oocytes and granulosa cells cultured in vitro.

Of follicular oocytes with germinal vesicles, 79% resumed meiosis within 48 hours in modified Ham's F-10 medium for oocyte culture, and 59% of them reached the second-metaphase stage of meiosis. An increase in cortical granules and tubular aggregates and a decrease in the Golgi apparatus were observed during the maturation process. Scanning electron microscopy of the oocyte surface revealed a decrease in number and length of microvilli. Nuclear DNA contents diminished to approximately one-half, but cytoplasmic protein contents measured by cytofluorometry were unchanged after culture in spite of these structural changes. Insemination with washed spermatozoa resulted in fertilization of 5 of 43 zona-intact cultured oocytes. After removal of the zona, 6 of 17 cultured oocytes showed polyspermic penetration, although immature oocytes were not fertilized even after removal of the zona. Cultured granulosa cells secreted progesterone spontaneously, and its amount was correlated with follicular size. Ultrastructurally, mitochondria with laminar cristae, enlarged nuclei, dispersed Golgi apparatus, smooth endoplasmic reticulum, lipid droplets in the ooplasm, and granular cytoplasmic protrusions were observed on the oocyte surface.

Cell Membrane↗
PubMed1981

[Relationship between the onset of labor and prostaglandins in rabbit tissues before and after delivery (author's transl)].

1) The studies were performed to elucidate the mechanism of onset of labor. It was investigated in relation to the movement of prostaglandins in individual tissues of pregnant rabbits before and after labor with the onset of labor. 2) The rabbit uterus duplex used is convenient material to investigate the movement of PGs in the same rabbit at the different term. 3) The concentrations of PGs were measured by RIA. 4) The concentrations of PGs in blood and amniotic fluid were not essential to explain account for the mechanism of onset labor. 5) From the results of the PGs values before and after labor, we have been considered that the relative PGs at the onset of labor were PG E1 and PG E2 in the placenta. We infer that PG F2 alpha plays a leading role in uterine contraction, because the large amount of PG F2 alpha presented in decidual parietalis during delivery, and PG E1 and PG E2 play a role in uterine contraction, too.

Amniotic Fluid↗
PubMed1980

Hepatocellular carcinoma in a case of Dubin-Johnson syndrome treated successfully with right extended lobectomy.

A 57-year-old man with hepatocellular carcinoma and liver cirrhosis combined with Dubin-Johnson syndrome was successfully treated by extensive right hepatic lobectomy. While the preoperative serum bilirubin level is one of the criteria in determining the indication for radical hepatic resection, it does not play a decisive role in the case of combined Dubin-Johnson syndrome.

Bilirubin↗
PubMed1980

Morphological changes of the liver in uremic patients treated with chronic hemodialysis--laparoscopic observations and light- and electron-microscopic studies.

In order to clarify morphological changes of the liver in the uremic state, 16 uremic patients treated with chronic hemodialysis were studied. Biopsy was performed in 14 cases under laparoscopic observation and in two on the occasion of renal transplantation. One uremic patients not being treated with dialysis was also studied for comparison. All biopsy specimens were examined by light and electron microscopy. The liver usually appeared mildly or moderately swollen under laparoscopic observations, which was considered at least partially due to the enlargement of the hepatocytes. All patients had hepatocytes with an Orcein-negative "ground glass" appearance, in which marked proliferation of smooth endoplasmic reticulum (SER) was found by electron microscopy. Since the patient not being on dialysis also had such hepatocytes, this finding may be characteristic of uremia. With electron microscopy, in addition to proliferation of SER, alteration of mitochondria and rough endoplasmic reticulum (RER) and an increase in cytoplasmic lipid droplets were observed. Hypertrophy of the Golgi apparatus containing electron-dense particles (VLDL) was often found in patients associated with hypertriglyceridemia. Amorphous electron-dense inclusions in microbodies were occasionally observed. Siderosis was observed in nine patients including three having parenchymal siderosis. With electron microscopy, various siderosomes were seen in the cytoplasm of hepatocytes in patients with parencymal siderosis. Conclusively, these histological and ultrastructral features of hepatocytes are rather associated with several metabolic abnormalities in uremia.

Adolescent↗