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Biomedical subjects

K Seki

Publications and source records attributed to K Seki.

At least 469 records · Page 26Linked to original sources
PubMed1982

[Estrogen receptors of the rat uterus during pregnancy and lactation].

Uterine estrogen receptors (ER) of the rat during pregnancy and postpartal lactation were assayed. Both the cytoplasmic and nuclear ER were determined by the controlled pore glass (CPG) method as previously described in detail. The cytoplasmic ER level slightly decreased as pregnancy advanced and then increased remarkably during postpartal lactation, with a tendency to decrease at 5 weeks of lactation when the estrous cycle could have recurred despite the nursing behavior of the rats. On the other hand, no remarkable difference in nuclear ER was noted during pregnancy and lactation. A high concentration of plasma progesterone could have reduced the translocation of the cytoplasmic ER to the nucleus and the replenishment of the cytoplasmic ER, despite a high concentration of plasma estrogen during pregnancy. In order to keep an environment of a high plasma concentration of estrogen similar to that of pregnancy, a long-acting estrogen (estradiol dipropionate) was injected in to the proestrous rats. The cytoplasmic ER rapidly translocated to the nucleus, followed by a prolonged maintenance of a low cytoplasmic and a high nuclear ER level until 7 days, with a gradual replenishment of the cytoplasmic Er and a gradual decline of the nuclear ER thereafter. A translocation of the cytoplasmic Er to the nucleus, similarly seen in the hysterectonized-adult rats treated with estradiol, was noted in the rats injected with estradiol at 3 weeks of lactation. In general, low estrogen and high prolactin may be held in plasma during lactation. These results indicate that the fluctuation of uterine ER simply depends on the plasma level of estrogen during pregnancy and postpartal lactation in rats.

Animals↗
PubMed1982

Hypertrophic and hypoactive smooth endoplasmic reticulum in hepatocytes uremic patients. A morphometric and biochemical study.

Morphometric analysis of the hepatic endoplasmic reticulum and assays of drug-metabolizing enzymes in microsomes prepared from needle biopsy specimens were performed with samples from uremic patients on chronic hemodialysis and normal controls. The smooth endoplasmic reticulum (SER) morphometrically increased in the uremic patients, whereas the cytochrome P-450 content and the activity of p-nitroanisole O-demethylase per mg microsomal protein decreased in the uremic patients. This condition of SER corresponds to that of the so-called hypoactive hypertrophic smooth endoplasmic reticulum, which could suggest lower activity of hepatic microsomal drug oxidation in uremic patients. On the other hand, the P-450 content and the activity of p-nitroanisole O-demethylase per g liver were not significantly different between the uremic and the normal subjects. This seems to indicate that the capacity of drug oxidation is retained in whole livers of uremic patients. However, since some patients in this study showed markedly low activity of p-nitroanisole O-demethylase per mg microsomal protein and per g liver, lipophilic drugs metabolized in the liver as well as hydrophilic ones eliminated by the kidney should be carefully administered to uremic patients.

Adult↗
PubMed1982

Laparoscopic findings of the liver in the diagnosis of primary biliary cirrhosis: "reddish patch", a laparoscopic feature in the asymptomatic stage.

The laparoscopic findings of the livers of 12 patients with primary biliary cirrhosis (PBC), including six asymptomatic and six symptomatic cases, were investigated. On the liver surface of asymptomatic PBC, we found a laparoscopic appearance, consisting of reddish patches of irregular shape, ranging from 0.5 to 3 cm in diameter, and not evenly distributed. Five of six cases of asymptomatic PBC had reddish patches in our series. From serial laparoscopic observations, the patches were found to lose some of their reddish tone and to develop protuberances with vaguely outlined white markings in each reddish areas. The protuberances and white markings are characteristic appearances of the liver surface in more advanced PBC cases. The transition of reddish patches to protuberances with white markings coincided with progression among histopathological stages in PBC classified by Scheuer. A histometrical examination showed that areas with reddish patches had less advanced changes of intrahepatic bile ducts. As a result, we propose that laparoscopic findings of the liver have a value in the diagnosis of PBC and "reddish patch" is considered a laparoscopic feature which has no been described in asymptomatic PBC.

Adult↗
PubMed1982

Stimulatory effect of cytochrome b5 induced by p-nitroanisole and diisopropyl 1,3-dithiol-2-ylidenemalonate on rat liver microsomal drug hydroxylations.

The relationship between the changes in the amount of the components of the liver microsomal electron transport systems and drug hydroxylase activities on administration of p-nitroanisole was investigated. The content of cytochrome P-450 in the p-nitroanisole-treated rats was not significantly different from that in the controls during the treatment. The cytochrome b5 content increased 2.2-fold over that of the controls after 14 days of treatment. Demethylation activities per mg microsomal protein of p-nitroanisole, aminopyrine, and benzphetamine were enhanced 2.7-, 2.4-, and 2.8-fold, respectively, but aniline hydroxylase activity was not enhanced. Similar results were obtained with microsomes from diisopropyl 1,3-dithiol-2-ylidenemalonate (NKK-105)-treated rats. The time-course study of changes in the amount of the components of drug hydroxylase systems suggested that the increase in cytochrome b5 content enhanced the demethylation activities. In the reconstituted system containing cytochrome P-450 partially purified from p-nitroanisole- or NKK-105-treated rats, cytochrome b5 was required for the maximal activities of the demethylation reactions, but did not participate in aniline hydroxylation. These findings showed good correlation between the change of cytochrome b5 content and the demethylation activities and suggested that the increase in cytochrome b5 content might enhance the demethylation activity by stimulating supply of a second electron to cytochrome P-450.

Animals↗
PubMed1982

Effects of a dopamine antagonist (metoclopramide) on the release of the adenohypophyseal hormones in amenorrhoeic patients with and without hyperprolactinaemia.

To obtain insight into the role of endogenous dopamine (DA) on the adenohypophyseal hormones release in amenorrhoeic women, responses of the adenohypophyseal hormones to a DA antagonist, metoclopramide (MCP), were evaluated in 4 normal women, in 9 normoprolactinaemic-amenorrhoeic patients, and in 7 hyperprolactinaemic-amenorrhoeic patients. The hyperprolactinaemic-amenorrhoeic patients were, in the majority, without a demonstrable microadenoma. Maximal net increase of prolactin (Prl) after MCP administration were lower (P less than 0.01) in hyperprolactinaemic patients compared with those seen in normal women or in normoprolactinaemic-amenorrhoeic patients. Administration of MCP resulted in significant elevation in serum FSH, LH and TSH in hyperprolactinaemic patients, but neither in normal women nor in normoprolactinaemic-amenorrhoeic patients. These findings suggest an increased dopaminergic inhibition of the release of TSH as well as FSH and LH in hyperprolactinaemic-amenorrhoeic patients.

Adult↗
PubMed1982

[Left ventricular hypertrophy in hypertensive and athlete's hearts: echocardiographic and vectorcardiographic characteristics].

The difference in the patterns of left ventricular hypertrophy (LVH) between the athlete's heart (Judo player: Judo-Ath group) and the hypertensive heart (HT group) was studied using vectorcardiography and echocardiography. The both groups were classified into two types based on the pattern of a QRS loop vector in the horizontal plane, respectively. One type (type II) satisfied the vectorcardiographic criteria of LVH by Upshaw, and the other (type I) did not satisfy it. The subjects composed of 12 Ath-I, 18 Ath-II, 10 HT-I and 16 HT-II. In the Ath-I, the characteristic vectorcardiogram was demonstrated in the horizontal plane, where an increased amplitude of an initial QRS vector was in the right and anterior direction. A mean ratio of the thickness of the interventricular septum to left ventricular posterior wall (IVSTd/PWTd) was 1.20 (p less than 0.001) in their echocardiograms. In HT-I, on the other hand, a decreased amplitude of an initial anterior vector and an increased amplitude of a posterior vector were observed in the horizontal plane. IVSTd and PWTd did not increase in these patients. These results indicated that an increased amplitude of an initial QRS vector in Ath-I is a reflection of the increased IVSTd. In type II, both IVSTd and PWTd were symmetrically increased. Concerning a spatial maximum QRS magnitude and left ventricular mass (LV mass), there was a significant correlation between the two only in HT-II (r = 0.75, p less than 0.01). It was concluded that there was some vectorcardiographic and echocardiographic differences between the left ventricular hypertrophic patterns of the athlete's heart and the hypertensive heart.

Adult↗
PubMed1982

[Changes in the criteria for planned delivery in the National Defense Medical College Hospital with statistical analysis].

Planned deliveries have been attempted with the administration of prostaglandins E2 and F2 alpha since 1978. Eight hundred and eighteen prostaglandins-induced and 729 uninduced deliveries from January, 1978 to July, 1980 were statistically analyzed. The uninduced deliveries included 465 spontaneous and 264 stimulated labors. The cervical ripening was considered the basic requirement stimulated labors. The cervical ripening was considered the basic requirement for labor induction for the first year of study. However, spontaneous labor onset or the membranes rupture occurred before the induction in 52.4% of cases, where babies were large enough (3,052.6 +/- 33.4g, mean +/- SE, n = 154). Therefore, the cervical factor was excluded from the essential requirement for induction. Since the beginning of 1979, labor induction has been performed based on gestational age (38 weeks or more) and uterine fundal length (32cm or more). Laminaria tents were inserted into the unfavorable cervix the night before induction. Delivery time in the day, duration of labor, and birth weight of babies in the induced group were 2.04 +/- 0.18 p.m., 8.0 +/- 0.3 hours and 3,138.8 +/- 12.3g, respectively. Apgar scores and the amount of postpartum hemorrhage were not significantly different from those in the uninduced group. Similar results were obtained between the laminaria subgroup with unfavorable cervix and the non-laminaria subgroup with ripe cervix in the induced primigravidas.

Birth Weight↗