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Biomedical subjects

K Kano

Publications and source records attributed to K Kano.

At least 289 records · Page 16Linked to original sources
PubMed1979

Hanganutziu-Deicher antigen and antibody in pathologic sera and tissues.

Heterophile, Hanganutziu-Deicher (H-D) antigen was studied in pathologic sera by means of inhibition of agglutination of bovine erythrocytes by H-D antibodies. H-D antigen was demonstrated in 38% of random cancer sera, 25% of lymphoma or leukemia sera, 25% of leprosy sera, 8% of infectious mononucleosis sera, 6% of rheumatoid arthritis sera, and 27% of synovial fluids of rheumatoid arthritis patients. None of 134 normal human sera gave positive results. Some of the inhibition-positive cancer sera formed precipitation lines with H-D antibody-containing sera. Over 50% of various extracts of cancer tissues as well as spleens of lymphoma or leukemia patients were shown to contain H-D antigen by means of the inhibition test.

Animals↗
PubMed1978

Pyranine as a sensitive pH probe for liposome interiors and surfaces. pH gradients across phospholipid vesicles.

Pyranine is shown to be a convenient and sensitive probe for reporting pH values, pHi, at the interior of anionic and at the outer surface of cationic liposomes. It is well shielded from the phospholipid headgroups by water molecules in the interior of anionic liposomes, but it is bound to the surface of cationic liposomes. Hydrogen ion concentrations outside the liposomes, 'bulk pH values', pHo, were measured by a combination electrode. While pHi = pHo for neutral, pHi less than pHo for anionic and pHi greater than pHo for cationic liposomes prepared in 5.0 . 10(-3) M phosphate buffers. pKa values for the ionization of pyranine were 7.22 +/- 0.04 and 6.00 +/- 0.05 in water and at the external surface of cationic liposomes. The surface potential for cationic liposomes containing dipalmitoyl-DL-alpha-phosphatidylcholine, cholesterol and octadecylamine in the molar ratio of 1.00 : 0.634 : 1.01, were calcuated to be +72.2 mV. Proton permeabilities were measured for single and multicompartment anionic liposomes. Transfer of anionic liposomes prepared at a given pH to a solution of different pH resulted in a pH gradient if sodium phosphate or borate were used as buffers. In the presence of sodium acetate proton equilibration is promptly established.

Amines↗
PubMed1978

Comparison of various procedures for the detection of antigen-antibody complexes.

The described test of inhibition of AA activity proved to be a procedure of exquisite sensitivity and specificity for detection of immune complexes. This could have been expected since AA is an antibody specifically combining with IgG antibody that suffered molecular transformation in the reaction with its corresponding antigen. Complexes formed at or close to the equivalence zone were most active in inhibiting AA, whereas those at extreme antigen excess did not inhibit it at all. Inhibition of AA was capable of detecting circulating immune complexes as well as immune complexes deposited in the tissues. It was also shown that by using this procedure, identification of the antigen participating in immune complex formation is feasible since excess of the specific antigen converts inhibitory complexes into inactive complexes.

Antibodies, Anti-Idiotypic↗
PubMed1978

Disappearance of the difference between adrenal A and NA cells in the level of radioactivity after 3H-dopa injection in the hypophysectomized mouse.

Radioactivity in adrenal medullary chromaffin cells of the mouse was examined by autoradiographic methods 15 min to 1 hr after an intraperitoneal injection of 3H-dopa. In the normal control mice, the concentration of radioactivity was significantly higher in the A cells than in the NA cells, whereas in the hypophysectomized mice (9 days after operation by the transauricular method) the radioactivity was evenly distributed in the two cell types. It was suggested that both A and NA cells possessed a special trapping and concentrating mechanism for extracellular dopa and/or its metabolites and that the activity of this mechanism was made greater in the A cells than in the NA cells by the pituitary gland.

Adrenal Medulla↗
PubMed1978

Reactions of rabbit antisera to human beta2-microglobulin (beta2-M) with platelets.

Reactions of rabbit antisera to human beta2-M against human platelets and man-mouse hybrid cells were studied by MA tests and cytolysis tests. Evidence is presented that the beta2-M act as species-specific cell surface antigens, some of which are most likely shared by cell surface proteins determined by genes on chromosomes other than the chromosome 15.

Absorption↗
PubMed1977

Fine structure of adrenal adenomata causing Cushing's syndrome.

Adenomata taken from nine patients with Cushing's syndrome were observed by electron microscopy. Agranular endoplasmic reticulum was prominently developed in all cases, occasionally fine dotty granules were observed in the tubules of agranular endoplasmic reticulum. Mitochondria showed a wide variety of changes in size, shape and internal structure, and somtimes contained electron dense droplets in their matrix. Mitochondria resembling those in the normal zona fasciculata intermingled with pathologically altered ones, suggesting that the adenomata which caused Cushing's syndrome were derived from the zona fasciculata. Fibrous structures were seen in the cytoplasm in one case. It is proposed that all of the changes in cellular organelles of the adenoma cells are correlated with increased secretory activity.

Adenoma↗
PubMed1977

Disseminated immune deposits in lupus erythematosus.

Immunohistologic studies were performed on autopsy tissues of 2 patients with systemic lupus erythematosus. All tissues examined--the kidney, lung, spleen, liver, intestine, peritoneum, and choroid plexus--contained immune deposits. Antinuclear antibody concentration in immunoglobulin G eluted from lung and spleen tissue was higher than in serum immunoglobulin G. These findings support the assumption that in systemic lupus erythematosus the renal as well as the extrarenal lesions can be attributed to vascular deposition of immune complexes.

Adolescent↗
PubMed1977

Isolation and partial characterization of the heterophile antigen of infectious mononucleosis from bovine erythrocytes.

The heterophile antigen (Paul-Bunnell antigen, PBA) of infectious mononucleosis was isolated by extraction of an aqueous suspension of bovine erythrocyte stromata with chloroform-methanol (2:1). The upper aqueous layer contained gangliosides, PBA, and a high-molecular-weight glycoprotein. PBA and gangliosides were separated from the high-molecular-weight glycoprotein by extraction of lyophilized upper layer with chloroform-methanol solvents. Separation of PBA from gangliosides was carried out by chromatography on DEAE-cellulose with chloroform-methanol solvents. PBA appeared to be a minor glycoprotein component of the erythrocyte membrane and had both hydrophobic and hydrophilic properties. It was soluble in either organic or aqueous solvents. On SDS-polyacrylamide gel electrophoresis, it migrated as a single component that stained for protein with Coomassie blue, for carbohydrate with periodic acid-Schiff reagent, and for lipid with oil red 0; it had an apparent molecular weight of 26,000. It was composed of 62% protein with major amino acids; glutamic acid, proline, glycine, isoleucine, leucine, and threonine (158, 116, 98, 90, 85, and 82 residues per 1,000 residues, respectively). Carbohydrate content was 9.2% with major sugar constituents: sialic acid, galactosamine, and galactose. Serologic activity of PBA was destroyed by pronase but not by trypsin.

Amino Acids↗