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Biomedical subjects

K Jinno

Publications and source records attributed to K Jinno.

At least 73 records · Page 4Linked to original sources
PubMed1987

Expression of ABH and Lewis blood group antigens in combined hepatocellular-cholangiocarcinoma. Possible evidence for the hepatocellular origin of combined hepatocellular-cholangiocarcinoma.

Expression of ABH, Lewis, and sialyl Lea antigens was studied in five combined hepatocellular-cholangiocarcinomas. Formalin-fixed liver tissues were immunostained for those antigens using well-characterized monoclonal antibodies and an avidin-biotin-peroxidase complex (ABC) method. Results were compared with those obtained in normal liver tissues and cholangiocarcinomas, and also with the previous observations of the authors on hepatocellular carcinomas. Although not detected in normal parenchymal liver cells, A, H, Lewis, and sialyl Lea antigens were found in combined hepatocellular-cholangiocarcinoma cells. Incompatible A antigen also was detected in one blood type O patient. Distribution and intensity of the antigens were similar to those in hepatocellular carcinomas and different from those in cholangiocarcinomas. No preferential accumulation of blood-group antigens could be found in the area of cholangiocarcinoma-like differentiation of the combined hepatocellular-cholangiocarcinoma. The observations suggested that Regional morphological differentiation of the hepatocellular-cholangiocarcinoma might not be always associated with the change in the expression of the blood group antigens. Moreover, the expression was essentially the same between the hepatocellular-cholangiocarcinoma and the typical hepatocellular carcinoma. The hepatocellular-cholangiocarcinoma, therefore could be a variant of the hepatocellular carcinoma.

ABO Blood-Group System↗
PubMed1987

Elution behaviour of peropyrene-type polycyclic aromatic hydrocarbons in liquid chromatography.

The elution behaviour of peropyrene-type polycyclic aromatic hydrocarbons (PAHs) on variously bonded stationary phases in non-aqueous reversed-phase liquid chromatography has been investigated. The results clearly indicate that the retention behaviour is effected by the degree of planarity of the PAHs and by the properties of the stationary phases used. Solute planarity might also be influenced by solvation. Therefore, these factors combine to determine the retention of large PAHs.

Chemical Phenomena↗
PubMed1987

Liquid chromatographic retention behaviour in the separation of anti-epileptic drugs on phenyl-, diphenyl-, and triphenyl-bonded silicas and glasses.

The retention and selectivity behaviour of some anti-epileptic drugs were studied by high-performance liquid chromatography on 21 kinds of phenyl-modified porous glasses and silicas, prepared from solutions of phenyldimethylchlorosilane, diphenylmethylchlorosilane or triphenylchlorosilane in xylene, and from various kinds of glass or silica with various mean pore diameters and/or specific surface areas. From elemental analysis data for carbon, the maximum number of bonded phenyl surface groups per gram (mean pore diameter 15 nm, specific surface area 217 m2/g, pore volume 0.85 ml/g) in phenyl-, diphenyl-, and triphenyl-bonded gels was calculated to be 0.313, 0.159, and 0.112 X 10(21), respectively. Using various acetonitrile-0.01 M potassium dihydrogen phosphate mixtures as eluents, the anti-epileptic drugs were separated on all the gels studied, but with different degrees of resolution. With increase in specific surface area on the glasses or silicas, the k' values of three anti-epileptic drugs increased. The selectivity for the separation of carbamazepine and diphenylhydantoin is discussed and explained by the pi-pi interaction between solutes and stationary phases. It has been shown that diphenyl and triphenyl phases are more suitable stationary phases for the selective separation of anti-epileptic drugs than monophenyl phases.

Anticonvulsants↗
PubMed1987

Elution behaviour of planar and non-planar polycyclic aromatic hydrocarbons on various chemically bonded stationary phases in liquid chromatography.

The elution behaviour of planar and non-planar polycyclic aromatic hydrocarbons has been studied on various stationary phases in reversed-phase and normal-phase liquid chromatography. The results show that the elution behaviours of the isomer sets which have distinctly different planarities such as triphenylene, triphenylmethane or o-terphenyl reflect the characteristics of the chemically bonded stationary phases. The phases studied included polymeric C18, monomeric C18 (with end capping), monomeric C18 (without end capping), triphenyl, naphthylethyl and pyrenylethyl phases.

Chemical Phenomena↗
PubMed1987

Elution behaviour of peropyrene-type polycyclic aromatic hydrocarbons in various chemically bonded stationary phases in reversed-phase liquid chromatography.

The elution behaviour of polycyclic aromatic hydrocarbons (PAHs, 13 of the 16 U.S. Environmental Protection Agency priority pollutants and peropyrene types) was studied on several chemically bonded stationary phases (octadecylsilicas, di- and triphenylsilicas, naphthylethylsilica and pyrenylethylsilica) under reversed-phase conditions. The results showed that the elution order of peropyrene-type PAHs is highly dependent on the degree of planarity of the solute and on the orderlines of the bonded phases, whereas no definite differences were found in the retention behaviour of the 13 small PAHs on various stationary phases. The characteristics of all the stationary phases could be classified by statistical cluster analysis.

Chemical Phenomena↗
PubMed1987

Neoexpression of ABH and Lewis blood group antigens in human hepatocellular carcinomas.

Expression of blood group ABH, Lewis, and sialylated-Lea antigens in human hepatocellular carcinomas and the adjacent nontumorous liver tissues was investigated with the use of seven monoclonal antibodies against these carbohydrate determinants. Chromatogram antibody-binding assay and solid-phase enzyme immunoassay of the upper-phase neutral glycolipids revealed the tumor-associated expression of blood group A-active glycolipids incompatible with blood-type status of the patients, a blood group A-active glycolipid with mobility on thin-layer chromatography between the known 6- and 8-sugar blood group A-active glycolipids in human erythrocytes, blood group H-active glycolipids, and blocked synthesis of Lea-active glycolipids with or without concomitant accumulation of Leb-active glycolipids. Immunohistochemical analysis of the fixed tissues with the use of an avidin-biotin-peroxidase complex method revealed blood group antigens in biliary epithelial cells but not in parenchymal liver cells. However, hepatocellular carcinoma cells in some cases expressed H and Leb antigens. Although only type 1 chain H antigen was detected in biliary epithelial cells, both type 1 and type 2 chain H antigens were found in hepatocellular carcinoma cells.

ABO Blood-Group System↗
PubMed1987

Combined hepatocellular and cholangiocarcinoma arising in a cirrhotic liver. Report of an autopsy case.

A rare case of combined hepatocellular and cholangiocarcinoma arising in a 56-year-old female is reported. The autopsy disclosed the presence of two different kinds of tumors in the right lobe of the liver, which showed advanced cirrhosis; a massive rubbery, ill-defined and whitish-yellow cholangiocarcinoma and a nodular soft, encapsulated and dark green hepatocellular carcinoma. They were adjacent to each other, but showed no intermingling. Only the massive cholangiocarcinoma had invaded the portal vein and showed several intrahepatic metastatic foci and hepatic, pancreaticoduodenal and perigastric lymph node metastases. Immunohistochemically, carbohydrate antigen 19-9 was strongly positive only for the cholangiocarcinoma component, explaining the high titer of this antigen in the serum on admission. On the basis of these findings, the possible morphogenesis of the tumor observed in the cirrhotic liver is discussed.

Adenoma, Bile Duct↗
PubMed1987

The clinical evaluation of the late potentials in patients with ventricular arrhythmias.

We investigated the recognition of late potentials in patients with and without organic heart diseases and spontaneous ventricular arrhythmias. None of the normal subjects had late potentials and patients with ventricular arrhythmias but no organic heart diseases, also had no late potentials as well as patients with idiopathic ventricular tachycardias. Late potentials in patients with idiopathic cardiomyopathy were noted more frequently in the dilated type than in the hypertrophic type, especially in those with high grades of ventricular arrhythmias. Patients with old myocardial infarctions had a higher rate of late potentials recognition in cases of sudden death or ventricular tachycardias. On the other hand, we observed lower rates in patients during early stage of acute myocardial infarction in spite of the evidence of a higher rate of ventricular electrical instability. There was no association between ejection fractions, wall motion scores and late potentials. However, a higher recognition of late potentials was found in patients with inferior or posterior myocardial infarction and ventricular aneurysm. We concluded that the late potential must be evaluated in each of the different groups of organic heart diseases in order to estimate the clinical value of ventricular arrhythmias.

Adolescent↗
PubMed1987

[An autopsied case of malignant fibrous histiocytoma of the spleen].

An auspsied case of malignant fibrous histiocytoma (MFH) is reported. The patient, a 53-year-old male had developed splenomegaly 1 year before admission. On admission, hepatosplenomegaly and ascites were seen, with prominent leucocytosis in the peripheral blood, and the patient died of cachexia. On autopsy, the primary neoplasma was found in the spleen, with metastasis to the liver, vertebrae, lymph nodes and peritoneum. Histologically, to tumor showed a storiform arrangement of tumor cells, with foamy or hemosiderin-faden cytoplasm in places, corresponding to MFH. As far as we know, there have been no reports of primary MFH of the spleen in Japan.

Histiocytic Sarcoma↗
PubMed1986

[Serum and tissue concentrations of UFT in patients with lung cancer].

Postoperative serum and tissue concentrations of 5-FU, FT-207 and uracil were measured in 36 patients with lung cancer who were administered UFT for seven days preoperatively. The concentration of 5-FU was high in tumor tissue and lymph nodes, but very low in serum. Such differences were not observed in the FT-207 levels. Tumor concentration of 5-FU in patients administered daily doses of 600 mg was 0.151 +/- 0.099 microgram/g which was three times higher than the minimum inhibitory concentration, and higher than that seen with other doses. The histological type and T factor were not related to the tissue concentration of 5-FU. Lymph node metastasis was not related to the concentration of 5-FU in the lymph nodes. The optimal daily dose of UFT for patients with lung cancer was considered to be 600 mg.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1986

[SAM sound studied by pulsed Doppler echocardiography].

The occurrence of a systolic sound in hypertrophic obstructive cardiomyopathy (HOCM) has been well known for more than 20 years. This was phonoechocardiographically regarded as the sound coincident with the abrupt halt of the systolic anterior movement (SAM) of the mitral valve echo, and it has been termed the SAM sound. A 58-year-old man with HOCM was admitted with right hemiplegia. He was found to have a SAM sound which waxed and waned in intensity, and at times moved earlier into systole. He was studied by cardiac catheterization, M-mode and two-dimensional Doppler echocardiography (pulsed, continuous wave and color flow Doppler methods). Asymmetric septal hypertrophy (interventricular septal thickness = 25 mm, left ventricular posterior wall thickness = 14 mm), as well as SAM and midsystolic aortic valve closure were demonstrated. The presence and intensity of the sound was not related to rhythm (normal sinus rhythm vs atrial flutter), heart rate, respiration, position, or inhalation of amyl nitrite. Two-dimensional Doppler echocardiography revealed the following: 1. In the left ventricular outflow tract just below the aortic valve, a systolic turbulent flow was always present. 2. In the left ventricular chamber near the apex, a systolic laminar flow was interrupted in those cycles where the SAM sound was present. Otherwise, in cycles lacking the SAM sound, laminar flow in this locality continued throughout systole (even shorter duration than normal). 3. In the left ventricular inflow tract, diastolic flow was unaffected by the presence of the sound. 4. No mitral regurgitation was observed using color flow Doppler echocardiography. In summary, a SAM sound appeared to be associated with sudden deceleration of blood flow from the apex to the mid left ventricle.

Cardiomyopathy, Hypertrophic↗