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Biomedical subjects

K Ikuta

Publications and source records attributed to K Ikuta.

At least 379 records · Page 21Linked to original sources
PubMed1982

Monoclonal antibodies specific to and cross-reactive with Marek's disease virus and herpesvirus of turkeys.

In order to identify the specific and cross-reactive antigens to Marek's disease virus (MDV) and herpesvirus of turkeys (HVT), we prepared hybridomas producing monoclonal antibodies against these viruses. These monoclonal antibodies were screened by the indirect immunofluorescence method. The purified virus-specific antigens appeared to be more effective immunogens than unpurified virus-infected cell homogenates. Totals of 50 and 14 hybridoma clones were found to produce antibodies specific to MDV and HVT, respectively. Of these, 14 MDV clones and 5 HVT clones produced antibodies that recognized antigenic sites common to both viruses.

Animals↗
PubMed1982

Heterophile Hanganutziu-Deicher antibodies in sera of patients with Kawasaki diseases.

The heterophile antibody levels in sera from patients with Kawasaki disease (49 sera from 39 cases) were measured by sheep erythrocytes (SRBC) agglutination and radioimmunoassay in microplates coated with Hanganutziu-Deicher (H-D) antigen-active glycosphingolipid, equine hematoside. The antibody levels were low in the first week of illness, increased rapidly in the 2nd week, and thereafter gradually decreased. The SRBC agglutination titers and H-D antibody titers of sera from patients with Kawasaki disease from week 2 to 8 of illness were significantly higher than those of healthy children (44 sera) and normal cord blood (13 sera).

Antibodies, Heterophile↗
PubMed1982

Hanganutziu-Deicher type-heterophile antigen-positive cells in human cancer tissues demonstrated by membrane immunofluorescence.

The possible expression of Hanganutziu-Deicher (H-D)-type heterophile antigen on the cell surface of several types of human cancer tissues was investigated by the membrane immunofluorescence test with anti-H-D serum. Anti-H-D serum of high titer was obtained from chickens immunized with H-D antigen-active glycosphingolipid, N-glycolylneuraminyl-lactosylceramide (equine hematoside). H-D antigen was demonstrated in 3 to 6 gastric cancer tissues, 2 of 3 lung cancer tissues, and 2 of 4 breast tissues examined, but not in 5 colorectal cancer tissues examined.

Antigens, Heterophile↗
PubMed1982

Hemopoietic colony-forming cells in Shwachman's syndrome.

Colony-forming capacities were studied in three Japanese children with Shwachman's syndrome (chronic neutropenia and exocrine pancreatic insufficiency). Bone marrow granulocyte-macrophage colony-forming cells assayed in a soft agar culture were markedly reduced in all three cases. The cytochemical examination of granulocyte-macrophage colonies by a new technique revealed that 90% of the colonies by a new technique revealed that 90% of the colonies consisted exclusively of granulocytes. Erythroid colony-forming cells assayed in a plasma clot culture were significantly reduced in two of the three cases. Bone marrow phagocytic cells did not suppress granulopoiesis in contrast to the cases of idiopathic aplastic anemia. Moreover, the patient serum did not inhibit granulopoiesis of normal bone marrow cells. These results have been discussed with the possibility of involving the hemopoietic stem cells and other additional factors.

Agranulocytosis↗
PubMed1981

Expression of Forssman antigen of avian lymphoblastoid cell lines transformed by Marek's disease virus or avian leukosis virus.

The expression of Forssman-type heterophile antigen on Marek's disease (MD) virus (MDV)-transformed cell lines, MDCC-MSBI 1, -HP1, -RP1 and -BP1, and avian leukosis virus (ALV)-transformed cell lines, LSCC-1104B1 and -1104X5 was investigated by membrane immunofluorescence and complement-dependent antibody cytotoxicity tests. Forssman antigen was detected on a high percentage of the cells in two ALV-transformed cell lines and on a smaller percentage of splenic lymphocytes from normal chicken. Of the MDV-transformed cell lines tested only the RP1 and BP1 cell lines, derived from transplantable MD tumours, expressed Forssman antigen, while the MSB1 and HP1 cell lines, derived from MD lymphomas, did not. Forssman antigen appears to be unrelated to MD tumour-associated surface antigen (MATSA).

Animals↗
PubMed1981

Latency of herpesvirus of turkey and Marek's disease virus genomes in a chicken T-lymphoblastoid cell line.

The properties of latent herpesvirus of turkey (HVT) and Marek's disease virus (MDV) genomes have been studied in virus-non-producer MDCC-BO1(T) cells, a T-lymphoblastoid cell line derived from spleen cells of an HVT-vaccinated chicken. The numbers of the two virus genomes in BO1(T) cells remained stable at 1.6 to 1.8 HVT genome equivalents/cell and 3.4 to 3.8 MDV genome equivalents/cell throughout a number of passages and were not decreased by the presence of phosphonoacetic acid in the culture. When the culture temperature of the MDV-producer MDCC-MSB1 cell line was shifted from 41 to 37 degrees C, the cells cultured at 37 degrees C contained about five times as many virus genomes as those cultured at 41 degrees C. In contrast, the numbers of the two virus genomes in BO1(T) cells were not increased by culture at 37 degrees C. RNA extracted from BO1(T) whole cells and from the polyribosomal fraction hybridized to both MDV and HVT DNAs, indicating the expression of both latent virus genomes. Digestion of cell nuclei with micrococcal nuclease revealed that both latent HVT and MDV genomes possess a nucleosomal structure. Closed circular MDV DNA was demonstrated in BO1(T) by isopycnic centrifugation of DNA in ethidium-bromide-CsCl gradients.

Animals↗
PubMed1981

Restriction endonuclease analysis of the genomes of virulent and avirulent Marek's disease viruses.

The DNAs from virulent strain BC-1 and avirulent strains C2(A) of Marek's disease virus (MDV) were compared by electrophoresis on 0.5% agarose gels of the products obtained with the restriction endonucleases Bam H1, Sal 1, and Sma 1. The patterns of the fragments of the DNAs from these two strains were very similar, but showed some significant differences in the number and mobility of the DNA bands. The DNA fragments obtained with the restriction endonucleases, and especially Sma 1, were mostly present in equal molar ratios, but a few of those obtained with Bam H1 and Sal 1 were present in submolar amounts. In addition, several fragments obtained with Bam H1 and Sal 1 were present in greater than molar quantities, suggesting the presence of reiterated sequences in MDV DNA. The terminal fragments of MDV DNA were identified by their sensitivity to lambda 5'-exonuclease. The terminal fragments obtained with Bam H1 A and Sal 1B showed heterogeneous electrophoretic mobility and contained sequences with high content of guanosine and cytosine, suggesting the presence of reiterated sequences at the end of the MDV DNA molecule.

Centrifugation, Isopycnic↗
PubMed1981

Prevention of syngeneic tumor growth in vaccinia virus-primed mice by immunization with vaccinia virus-modulated tumor cells.

Immunization with vaccinia virus-infected and then X-ray-irradiated murine hepatoma MH134 cells provided C3H/He mice with strong resistance to challenge with viable MH134 cells. Male C3H/He mice of 5 to 6 weeks old were primed intraperitoneally (IP) with 1 x 10(7) PFU of live vaccinia virus (Ikeda strain) after irradiation with 250 R of X-ray. Three weeks after priming, the mice were immunized IP 3 times at weekly intervals with 1 x 10(7) X-ray-irradiated MH134 cells that had been infected with vaccinia virus 8 h before irradiation. Over 60% of these cells showed vaccinia virus-induced antigen on their surface (membrane antigen). Challenge with viable MH134 cells was done by inoculating 1 x 10(5) cells IP one week after immunization. During a 4-week observation period, all the untreated control mice died with ascites. On the contrary, all the mice that were X-ray-irradiated, primed and immunized survived challenge with the tumor cells for at least 4 weeks. The mortalities of mice in other groups that were not irradiated, or not primed, or immunized with only X-ray-irradiated tumor cells, were at lowest 50%.

Animals↗
PubMed1980

Biochemical studies on early cell surface antigen induced by vaccinia and cowpox viruses.

The early cell surface antigen (ECSA) induced by vaccinia or cowpox virus infection was analysed by immunoprecipitation and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), The components with a mol. wt. of about 40 000 (40 K) in vaccinia virus-infected cells and with a mol. wt. of about 43 000 (43 K) in cowpox virus-infected cells were specifically immunoprecipitated with anti-ECSA serum prepared by immunizing rabbits with rabbit kidney cells infected with a conditional lethal mutant. The 40 K and 43 K polypeptides were synthesized even in the presence of cytosine-I-beta-D-arabinofuranosyl-HCl (Ara C) and were detectable even in the cells pulse-labelled between I and 2 h p.i.

Animals↗
PubMed1980

Persistence of genomes of both herpesvirus of turkeys and Marek's disease virus in a chicken T-lymphoblastoid cell line.

A cell line tentatively designated as MDCC-BO1(T), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (HVT). BO1(T) cells were T lymphoblastoid cells and the more than 95% of them had Marek's disease (MD) tumor-associated surface antigen (MATSA). However, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-HVT and -MD virus (MDV) sera. The virus could be rescued from BO1(T) cells by co-cultivation with chick embryo fibroblasts (CEF). The DNA of the rescued virus was characterized as HVT DNA by its sedimentation profile in a neutral glycerol gradient and its endonuclease Hind III cleavage-pattern. Ultrastructural studies on CEF infected with the rescued virus revealed the presence of HVT-like virions. However, DNA-DNA reassociation kinetics showed that the BO1(T) cells contained a few copies of NVT and also MDV genomes.

Animals↗
PubMed1980

Demonstration of heterophile antibody in chicken antiserum against Marek's disease tumor-derived cell line, MSB-1.

Sheep red blood cells (SRBC) were agglutinated by all six chicken anti-MSB-1 sera examined, but not by sera of thirty specific pathogen-free chickens. The SRBC-agglutination titer was greatly reduced by absorption with SRBC, bovine red blood cells (BRBC) or guinea-pig kidney cells (GPKC). The dissociation of heterophile antibody and antibody to so-called Marek's disease tumor-associated surface antigen (MATSA) is discussed.

Animals↗
PubMed1980

Distribution of Forssman antigen in chickens.

The distribution of Forssman antigen in various tissues of chickens was studied by the indirect and direct immunofluorescence (IF) tests using rabbit immune serum against purified Forssman glycosphingolipid (GSL) [GalNAc (alpha, 1-3)GalNAc(beta, 1-3)Gal(alpha, 1-4)Gal(beta, 1-4)Glc-ceramide]. A general finding in all tissue sections was the presence of Forssman antigen in vascular endothelium and perivascular connective tissues. Erythrocytes gave a Forssman positive reaction, but the intensity of fluorescence was slight. Forssman antigen was found in about 20% of all peripheral leukocytes. Fractionation of lymphocytes from peripheral blood into T and B lymphocytes showed that about 30% of the B lymphocytes gave a positive reaction, while only 2-3% of the T lymphocytes gave a positive reaction. About 40% of bone marrow cells expressed Forssman antigens. On the other hand, about 40-50% of adherent cells in vitro cultures from spleen were Forssman-positive. The antigen was also abundant in hematopoietic organs, such as spleen, thymus and the bursa of Fabricius. Positive reactions in the digestive tract, ovary, testis and skin varied from portion to portion of the tissues. In brain and spinal cord, only the vascular endothelium gave a positive reaction. In peripheral nerves, only the connective tissue stroma around the nerve fibers gave a positive reaction. In other tissues, the distribution of the antigen was generally in accord with the results of Tanaka and Leduc (1956). These positive reactions in the IF test were specifically inhibited by addition of purified Forssman GSL. The distributions of Forssman antigen in tissues were found to be the same in chickens of various ages.

Animals↗