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Biomedical subjects

K Ikuta

Publications and source records attributed to K Ikuta.

At least 361 records · Page 20Linked to original sources
PubMed1984

Cross-reactivity between cowpox and vaccinia viruses with monoclonal antibodies. Brief report.

Hybridoma cell lines producing monoclonal antibodies against cowpox virus (CPV) and vaccinia virus (VV) were established to examine the specific and cross-reactive antigenic determinants of these viruses. Monoclonal antibodies against CPV (LB red strain) and VV (Lister strain and Ikeda strain) were classified into several groups on the basis of the results of immunofluorescence and haemagglutination inhibition tests. It was suggested that the groups defined above include the group of antibodies reacting with each of known major antigens of poxvirus, i.e. nucleoprotein (NP) antigen, heat labile and stable complex (LS) antigen, haemagglutinin (HA), cell surface antigen (CSA) and type A inclusion body (A).

Antibodies, Monoclonal↗
PubMed1984

Studies on the serological cross-reaction between Marek's disease virus and herpesvirus of turkeys using monoclonal antibodies to major virus-specific polypeptides. Brief report.

A major virus-specific polypeptide antigenically cross-reactive between Marek's disease virus (MDV) and herpesvirus of turkeys (HVT) was characterized by two monoclonal antibodies against MDV. The antigenic determinant recognized by one monoclonal antibody was present only in the MDV polypeptide but not in the HVT polypeptide, whereas the antigenic determinant recognized by the other antibody was present in both MDV and HVT polypeptides. Peptide mapping showed the difference in the amino acid sequence of the two polypeptides.

Amino Acid Sequence↗
PubMed1984

Characterization of N-glycolyneuraminic acid-containing glycosphingolipids from a Marek's disease lymphoma-derived chicken cell line, MSB1, as tumor-associated heterophile Hanganutziu-Deicher antigens.

Heterophile, Hanganutziu-Deicher (HD) antigen-active N-glycolylneuraminic acid-containing glycosphingolipids (GSLs) were detected as tumor-associated foreign antigens of a Marek's disease lymphoma-derived cell line, MSB1, by enzyme-immunoassay with chicken antibody against N-glycolylneuraminyl-lactosylceramide (anti-NeuGc-LacCer). At least three species of HD antigen-active GSLs were detected by two-dimensional thin-layer chromatography (TLC) combined with enzyme-immunoassay. The reactivities of the GSLs with anti-NeuGc-LacCer, their behaviors on two-dimensional TLC and the results of an endo-beta-galactosidase digestion study indicated that these three GSLs were NeuGc-LacCer (NeuGc alpha 2-2Gal beta 1-4Glc-Cer), NeuGc-nLcOse4Cer (NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-Cer) and NeuGc-nLcOse6Cer (NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-Cer).

Animals↗
PubMed1984

Evaluation of DNA homology of Marek's disease virus, herpesvirus of turkeys and Epstein-Barr virus under varied stringent hybridization conditions.

Marek's disease virus (MDV) showed only 0.3-0.6% homology in DNA sequence with herpesvirus of turkeys (HVT) at Tm-24.4 degrees C in spite of its antigenic similarity to the latter. Southern blot hybridization under stringent conditions showed that the homology between MDV and HVT is located in the restricted portion of these viral genomes. At Tm-49.6 degrees C, which permits the detection of homology with one base mismatch in three between the MDV and HVT DNAs, sequences with weak homology were found to be distributed over most of these viral genomes. No homology was detected between Epstein-Barr virus and either MDV or HVT DNA.

Animals↗
PubMed1984

Processing of glycoprotein gB related to neutralization of Marek's disease virus and herpesvirus of turkeys.

The glycoprotein gB related to neutralization of Marek's disease virus (MDV) and herpesvirus of turkeys (HVT) is composed of several glycosylated polypeptides, which were immunoprecipitated with monoclonal antibodies and rabbit antiserum cross-reactive to MDV-gB and HVT-gB, and analyzed by SDS-polyacrylamide gel electrophoresis. The present pulse-chase experiments showed that the precursor forms of MDV- and HVT-gB were glycoproteins with molecular weights of 110K to 115K (gp115/110) and 115K (gp115), respectively. These precursor forms were processed to smaller gB's (gp63 and gp50 for MDV; gp62, gp52, and gp48 for HVT), at least in part by sialylation. The proteins synthesized in the presence of tunicamycin were two polypeptides of 88K and 83K in MDV-infected cells and a 90K polypeptide in HVT-infected cells, indicating the presence of unglycosylated precursor forms of MDV- and HVT-gB. Differences between virulent and avirulent MDV's and between HVT's with and without protective activity against Marek's disease were observed in the processed forms of MDV- and HVT-gB, especially at the processing step of sialylation.

Animals↗
PubMed1984

Identification with monoclonal antibodies of glycoproteins of Marek's disease virus and herpesvirus of turkeys related to virus neutralization.

By use of monoclonal antibodies cross-reactive with Marek's disease virus and herpesvirus of turkeys, three glycoproteins (for Marek's disease virus, gp115/110, gp63, and gp50; for herpesvirus of turkeys, gp115, gp62 and gp52) related to virus neutralization were identified. Immunization of chickens or rabbits with these glycoproteins purified by affinity chromatography resulted in production of neutralizing antibodies.

Animals↗
PubMed1984

Studies on experimental immune complex nephritis (3). Therapeutic effect of prostaglandin E1 alpha-cyclodextrin host molecule (PGE1 . CD) on serum sickness nephritis in rats.

The effect of prostaglandin E1 alpha-cyclodextrin host molecule (PGE1 . CD) was estimated by using a model of immune complex glomerulonephritis induced in rats by i.v. injection of 1 mg of rabbit serum albumin every other day. PGE1 . CD was given subcutaneously for 10 days after nephritis was definitely induced and resulted in a rapid restoration of various biochemical parameters, especially in plasma. The continuous s.c. administration of PGE1 . CD (300 micrograms/rat/day) with a mini osmotic pump provoked a therapeutic effect similar to that obtained with twice daily s.c. administration of PGE1 . CD (300 micrograms/rat X 2/day). Both PGE1 . CD groups revealed less glomerular damage and fewer locations of immune complexes in glomeruli as demonstrated by light and immunofluorescence microscopy. The beneficial effect of PGE1 . CD may be associated with reduced immune complex deposits in glomeruli. The present studies suggest that PGE1 . CD may enhance the clearance of immune complex deposits from the glomeruli rather than inhibiting the formation of immune complex in the circulation.

Acetylglucosaminidase↗
PubMed1984

Tumor-associated expression of glycosphingolipid Hanganutziu-Deicher antigen in human cancers.

Expression of heterophile Hanganutziu-Deicher (HD) antigen on the cell surface of various human malignant tumor tissues was studied by membrane immunofluorescence staining with chicken antiserum against N-glycolylneuraminyllactosylceramide (HD3) and fluorescein-conjugated rabbit anti-chicken IgG. The HD antigen was demonstrated in samples from 38 of 77 (38/77, 49%) cases, consisting of gastric (9/16), breast (8/14), colorectal (3/12), nasopharyngeal (4/7), and uterine (2/3) carcinomas, leukemias (5/10), malignant lymphomas (2/5), and other cancers (5/10). Histological examination indicated that expression of the antigen by the gastric, colorectal and breast carcinomas and leukemias was not related to their histological types. The cytoplasm and the surface of the malignant glandular epithelial cells were both specifically stained by immunofluorescence staining of frozen sections in one colon adenocarcinoma. Glycosphingolipids (GSLs) were extracted from the colon cancer tissue and two HD antigen-active GSLs were detected on thin-layer chromatography (TLC) by enzyme-immunostaining using affinity-purified chicken anti-HD3 and horseradish peroxidase-conjugated rabbit anti-chicken IgG. One migrated to the same position as HD3 on TLC, and the other to a position similar to that of authentic N-glycolylneuraminylneolactotetraosylceramide.

Antigens, Heterophile↗
PubMed1984

Isolation of monoclonal antibodies reactive with Marek's disease tumor-associated surface antigen (MATSA).

Two hybridoma clones producing monoclonal antibodies were obtained from mice immunized with the Marek's disease (MD)-lymphoblastoid cell line MSB1. These monoclonal antibodies reacted with the surface of MD-lymphoblastoid cell lines at higher titers than with avian lymphoid leukosis cell lines or with normal chicken thymus, bursa or peripheral blood lymphocytes. The serological specificity of these monoclonal antibodies seemed to correspond with that of rabbit antiserum reactive with MD tumor-associated surface antigen (MATSA).

Animals↗
PubMed1983

Monoclonal antibodies reactive with the surface and secreted glycoproteins of Marek's disease virus and herpesvirus of turkeys.

Hybridomas were formed between mouse myeloma cells and spleen cells from mice immunized with Marek's disease virus (MDV) or with herpesvirus of turkeys (HVT). Three monoclonal antibodies were obtained, two (M26 and M34) from MDV clones and one (H9) from an HVT clone, all of which were specific for cross-reactive membrane antigen (MA) expressed on the surface of cells infected with MDV or HVT. All three antibodies also reacted with MDV- and HVT-specific glycoproteins in the molecular weight (mol. wt.) ranges 54K to 70K (MDV-gp54/70) and 50K to 64K (HVT-gp50/64), respectively. These glycoproteins constitute the putative 'A' antigens which are found in the medium of cultures infected with MDV or HVT. These results suggest that the cross-reactive MA may correspond to 'A' antigen. Pulse-chase experiments using monoclonal antibodies revealed the presence in virus-infected cells of precursor and processed forms of MDV-gp54/70 and HVT-gp50/64 which differ in size. Moreover, by two-dimensional gel electrophoresis we found that MDV and HVT glycoproteins were separated to heterogeneous spots by electric charge as well as mol. wt. The several spots with higher mol. wt. and with more acidic isoelectric points among them were lost by treatment with neuraminidase, suggesting that the processing was, at least in part, due to the addition of sialic acid to the precursor forms. Tunicamycin blocked the surface expression of cross-reactive HVT-MA on HVT-infected cells. Phosphonoacetic acid inhibited both the appearance of HVT-MA on the cell surface and synthesis of HVT-gp50/64, indicating that the MA and secreted glycoprotein were late gene products of the HVT genome.

Animals↗
PubMed1983

Most virus-specific polypeptides in cells productively infected with Marek's disease virus or herpesvirus of turkeys possess cross-reactive determinants.

Most virus-specific polypeptides in cells productively infected with Marek's disease virus (MDV) or herpesvirus of turkeys (HVT) possess cross-reactive antigenic determinants, although the two viruses have very little DNA homology. The cross-reactivity appeared to be more evident when [35S]methionine-labelled polypeptides were immunoprecipitated than when the [3H]glucosamine-labelled polypeptides were immunoprecipitated, suggesting that the glycoproteins of MDV and HVT may be less related in structure than other proteins. The major cross-reactive glycoproteins excreted into the culture mediums of MDV- and HVT-infected cells had molecular weights of 64 000 (gp64) and 56 000 (gp56) respectively.

Animals↗
PubMed1982

Specificities of human heterophilic Hanganutziu and Deicher (H-D) antibodies and avian antisera against H-D antigen-active glycosphingolipids.

The precise specificities H-D antibodies and newly obtained avian antisera to the H-D antigen-active glycosphingolipids. N-glycolylneuraminyl (alpha 2-3) lactosyl ceramide (hematoside) and N-glycolylneuraminyl (alpha 2-3) lactoneotetraosyl ceramide (sialosylparagloboside) were compared by gel precipitation and hemagglutination inhibition using other gangliosides with a related structure and chemically modified hematoside derivatives and the oligosaccharides from both the antigenic glycosphingolipids. Human H-D antibodies were found to have the same affinity for both antigenic glycosphingolipids; however, the avian anti-hematoside or anti-sialosylparaglobosides showed a higher affinity for the homologous antigen. Human H-D antibodies were directed more exactly toward the terminal sialic acid residue and more roughly to the hydrophobic portion of both compounds; however, the two avian antibodies were directed more specificity to the whole structure of the homologous antigen glycosphingolipid.

Animals↗