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Biomedical subjects

K Harada

Publications and source records attributed to K Harada.

At least 1,279 records · Page 71Linked to original sources
PubMed1977

Staining mycobacteria with periodic acid-carbol-pararosanilin: principle and practice of the method.

Mycobacteria may be acid-fast, non-acid-fast or even chromophobic in staining under different conditions. The pretreatments with oxidants including periodic acid increase effectually the acid-fastness of acid-fast bacilli. This is caused by additional free carboxyl groups resulting from non-acid-fast wax in the cell walls by demethylation with oxidants. Only by prolonged periodic oxidation the aldehyde groups formed as oxidation products of 1-amino-2-hydroxy groups in the cells can be demonstrated with carbol-fuchsin stain. This reaction is most probably attributable to the formation of Schiff's bases between fuchsin and aldehydes. Since pararosanilin is the active molecule in the diphenamine reaction, periodic acid (10%, 24 hours) followed by carbol-pararosanilin stain is a most sensitive and selective method to demonstrate mycobacteria including chromophobic forms.

Animals↗
PubMed1977

Gelation of the amoebocyte lysate of Tachypleus tridentatus by cell wall digest of several gram-positive bacteria and synthetic peptidoglycan subunits of natural and unnatural configurations.

Water-soluble adjuvant-active fractions prepared by digestion of the cell walls from several gram-positive bacteria with an endo-N-acetylmuramidase (Mutanolysin) were shown to gelate the amoebocyte lysate of Tachypleus tridentatus, the Japanese horseshoe crab. This finding was further extended by examination of the clotting activities on the amoebocyte lysate of a synthetic N-acetylmuramyl-L-alanyl-D-isoglutamine and related compounds, either adjuvant-active or -inactive, demonstrating that there are in general close correlations between the abilities to initiate lysate gelation, immunoadjuvancies and pyrogenicities, although there are some exceptions.

Adjuvants, Immunologic↗
PubMed1976

Echocardiographic features of congenital left ventricular inflow obstruction.

The echocardiographic features of congenital left ventricular inflow obstruction are described in six patients. The echocardiograms in two patients with cor triatriatum were distinguished by normal mitral valve motion and an abnormal echo within the left atrium. In two patients with supravalvar mitral ring, in addition to abnormal mitral valve motion, an abnormal echo, presumably originating from the obstructive membrane, was located between the anterior and posterior mitral leaflets. In two cases of parachute mitral valve, mitral valve motion was abnormal. In one of these cases there were multiple mitral valve echoes similar to those found in supravalvar mitral ring. The echocardiographic identification of an obstructive membrane within the left atrium is difficult because of the occurrence of artifacts. However, membranes may be identified if careful scanning techniques are employed in patients in whom left ventricular inflow obstruction is suspected. The echocardiogram is useful in detecting mitral valve abnormalities in these patients and is valuable in cases where mitral valve replacement is contemplated.

Adolescent↗
PubMed1976

An evaluation of the function of new special equipment for transrectal ultrasonotomography.

A new type of equipment for transrectal ultrasonotomography was described in a previous paper. This equipment was evaluated from the data obtained from 205 cases in which examinations were performed. Tomograms were successfully recorded in 94% of cases. Tomograms sufficiently useful for diagnosis and the measurement of organs were obtained in 89% of cases for the prostate, 78% for the urinary bladder, and 80% for the seminal vesicles. No basic problems in the procedure were found, although hypotension of unknown origin was observed during the examination in a few cases.

Adolescent↗
PubMed1976

The nature of mycobacterial acid-fastness.

Phenol is not essential to acid-fast staining, for it will occur in the absence of phenol where such lipoid-soluble basic dyes as night blue, Victoria blue B or Victoria R are used; it is essential for acid-fast staining with water soluble basic dyes such as basic fuchsin. When phenol is added to the staining solution, such water soluble basic dyes behave in effect like their lipid-soluble counterparts. The loss of mycobacterial acid-fastness with carbol-fuchsin after bromination or chromation indicates that this phenomenon is related to the presence of unsaturated lipids in the bacterial cells. Within the cells these acid-fast lipids are bound in such a way that they are easily removed from all mycobacteria by hot dilute HCl; from leprosy bacilli alone they are easily removed with hot pyridine. From the results of various blocking reactions it appears that carboxyl and especially hydroxyl groups of these cellular lipids are essential to the acid-fast reaction of mycobacteria.

Lipids↗