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Biomedical subjects

K Harada

Publications and source records attributed to K Harada.

At least 1,297 records · Page 72Linked to original sources
PubMed1976

Staining mycobacteria with carbolfuchsin: properties of solutions prepared with different samples of basic fuchsin.

Acid fast staining of mycobacteria in the form of beadings is obtained by means of a carbolfuchsin solution (Ziehl-Neelsen stain) prepared from pararosaniline or from certain kinds of basic fuchsin. After such acid-fast stains, the intensity of the bacilli's colouring was rather poor and unstable, so that some bacilli lost their acid-fast stain. In contrast, an acid-fast staining of mycobacteria in rod form results by using a carbolfuchsin prepared from rosaniline or from other basic fuchsins included new fuchsin. The spectrophotometric and thin-layer chromatographic data indicate that the main component of those basic fuchsins showing beady staining may be pararosaniline, whereas the main ingredient of basic fuchsin with staining the bacteria in rod form may be its higher homologues. Neither chloride nor acetate of the fuchsin could affect the appearance and number of stained bacilli. The commercially available "basic fuchsin" is either the chloride or acetate of pure pararosaniline or consists of variable mixtures of it with higher homologues. Consequently, only a basic fuchsin which has an absorption maximum at lambda greater than or equal to 552 nm could be employed for the acid-fast stain of mycobacteria in a stable manner. Pararosaniline included some basic fuchsins, composed mainly from pararosaniline, should not be selected for the preparation of the carbolfuchsin formula.

Animals↗
PubMed1976

Correlation between the immunoadjuvant activities and pyrogenicities of synthetic N-acetylmuramyl-peptides or -amino acids.

A total of 14 different N-acetylmuramyl-peptides or -amino acids with or without configurations inherent to bacterial cell wall peptidoglycans were synthesized and their pyrogenicities on intravenous injection into rabbits were tested. N-Acetylmuramyl-peptides, and especially N-acetylmuramyl-L-alanyl-D-isoglutamine and N-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-lysine, which were previously shown to be adjuvant-active in both induction of delayed-type hypersensitivity and stimulation of increased serum antibody levels to ovalbumin in guinea pigs, exhibited distinct pyrogenicity at as low dose as 16 mug per rabbit. However, none of the adjuvant-inactive analogues or diastereomers of the above N-acetylmuramyl-dipeptide or related compounds caused any significant febrile response in rabbits, even at a dose of 250 mug per animal.

Adjuvants, Immunologic↗
PubMed1975

[The effect of bleomycin on brain tumor cells in vitro (author's transl)].

In order to evaluate the efficacy of Bleomycin (BLM) against brain tumor, a study was made on the inhibitory effect of BLM on the proliferation of cells derived from 8 cases of astrocytoma and 2 cases of meningioma and the proliferation of Ehrlich ascitic, HeLa and Mouse-Foot-Pad cells of strained epithelial origin. Morphological changes induced by BLM were also observed with phase contrast microscope and scanning electron microscope. Actively growing monolayer cells were exposed to BLM whose concentration was adjusted to 0.15, 0.6, 2.5, 10, 40, 160 mug/ml in the cultivating medium for 7 days. The inhibitory effect on cellular proliferation was evaluated by calculating the number of cell nuclei. When the concentration of BLM was adjusted to 0.15 mug/ml, the inhibition of Ehrlich ascitic, HeLa and Mouse-Foot-Pad cells was greater than the control by 50%. On the contrary, a higher concentration of BLM, that is 0.6-10 mug/ml, was necessary in the case of brain cells. The latter cells appear to be generally less sensitive to BLM than the foregoing strained cells of epithelial origin. Morphological changes consisted of cytoplasmic vacuolation, shortening and narrowing of cytoplasmic processes, nucleolysis and flattening of the surface structure. These findings, however, were not confirmed to be changes specific to BLM. Lastly, a brief review of the literature and discussion were made on the methods of administration and tissue concentration of BLM.

Adolescent↗
PubMed1975

Immunological and electrophoretical approaches to macroamylase analysis.

The characteristics of abnormally large-size amylase (macroamylase) of the three patients were studied. Amylase isoenzyme patterns of the three macroamylase samples of agar gel electrophoresis were different from the normal one: a post-beta-globulin or middle-ganna-globulin zone was found in macroamylase, while a fast-gamma, and a pre-gamma-globulin or both zones is found in normal amylase. With gel chromatography at pH 3.4, macroamylase dissociated to normalsized amylase. The electrophoretic patterns of the normal-sized amylase were identical with those of normal amylase. The binding proteins of the macroamylase were identified as immunoglobulin IgG in onecase and immunoglobulin IgA in the other two cases by the immunochemical criteria and immunoenzyme electropherogram. These immunoglobulins bound with serum amylase having normal molecular weight and electophoretic mobility to form macroamylase.

Amylases↗
PubMed1975

Transrectal ultrasonotomography of the prostate.

New equipment for transrectal ultrasonomography has been developed. Wth this equipment an excellent cross-section picture of various intrapelvic organs is easily obtained. Diagnostic application of the technique is particularly significant for abnormalities of the prostate because the shape of the prostate cannot be revealed by x-ray. Diagnostic criteria for prostatic disease are described, with the demonstration of typical cases. In a test by well trained physicians the diagnostic accuracy of the new technique was more than 80 per cent. Ultrasonotomography provided for precise measurement of prostatic size. Transrectal ultrasonotomography is a promising new diagnostic technique that yeilds abundant information not available with former methods of examination. The procedure is likely to become one of th most important diagnostic tools in the field of urology.

Male↗
PubMed1975

Volume estimation of the seminal vesicles by means of transrectal ultrasonotomography: a preliminary report.

The volume of the seminal vesicles was estimated in 27 cases by means of transrectal ultrasonotomography. The seminal vesicles of normal subjects were found to be generally larger than in cases of prostatic hypertrophy or cancer. The volume of the seminal vesicles in Klinefelter's syndrome was remarkably small. The correlation between the volume of the seminal vesicles and of the prostate is given.

Humans↗
PubMed1975

[The effect of nasal obstruction on the susceptibility of mice to noxious gases (author's transl)].

The effect of nasal obstruction on mice--the maice were forced to breathe through the mouth--was studied when the animals were exposed to noxious gases. It was noted that the forced mouth breathing made the mice less vulnerable to hydrogen sulfide, carbon monoxide and toluen vapor when expressed by the time interval before they lost righting reflex or showed respiratory arrest. The levels of the gases which could induce these sings within 5-10 minutes were significantly elevated with the "mouth breathing" mice. Possible mechanisms for this phenomenon and its application for men were discussed.

Animals↗
PubMed1975

[A case of "membranous lipodystrophy (Nasu)" with emphasis on psychiatric and neuropathologic aspects (author's transl)].

A housewife, 42 years old, died from a chronic progressive neuro-psychiatric illness of 15 years duration characterized by memory disturbance, moria-syndrome, euphoria, social disorder and extrapyramidal symptoms combined with a severe bone disease. Pathologically, the adipose tissue in bone marrow, and also in other body areas such as the periadrenal region and renal pelvis showed a characteristic degeneration with "membranous cycle" structures. Neuropathological examination showed an extensive atrophy of cerebral white matter, especially in the frontal and temporal lobes, with a marked fibrillary gliosis, a moderate destruction of myelin sheath without grossly visible distinct demyelination and with a relatively normal cerebral cortex: a picture resembling sclerosing leukoencephalopathy. From these findings the case was identified as "membranous lipodystrophy--Nasu." Moreover, the case may be related to the "lipomembranous polycystic osteodysplasia with progressive dementia" of Järvi, Hakola et. al. In cases with an unknown atrophic illness of cerebral white matter there should be carried out a pathological examination of adipose tissue, especially of the bone marrow.

Adipose Tissue↗