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Biomedical subjects

K Fukuda

Publications and source records attributed to K Fukuda.

At least 919 records · Page 51Linked to original sources
PubMed1983

[Histopathological evaluation of transarterial one shot infusion of adriamycin as a preoperative adjuvant chemotherapy for the breast cancer].

Thirty patients with resectable breast cancer were treated with transarterial one shot infusion of 20 mg of adriamycin as a preoperative adjuvant chemotherapy. After breast angiography by Seldinger's method, adriamycin at a dose of 20 mg was administered through the feeding artery with one shot injection. Anticancer effect of adriamycin was evaluated microscopically after radical mastectomy. In the primary tumor, the effective histological changes were found in 9 (30%) of 30 cases. In the metastatic lymph nodes, the effective changes were found in 5 (50%) of 10 cases. Mild side effects were seen in 70% of the cases, but not serious. It was suggested that transarterial one shot infusion of 20 mg adriamycin was a histopathologically effective procedure as preoperative chemotherapy for breast cancer.

Adult↗
PubMed1982

Structural studies of the acidic oligosaccharide units from bovine glycophorin.

The O-glycosidically-linked carbohydrate units of glycophorin from bovine erythrocyte membrane were released by alkaline borohydride treatment. These oligosaccharides were separated into the neutral fractions and the acidic fractions by ion-exchange chromatography followed by gel filtration. The two acidic fractions (fractions 10 and 13) which have the smallest molecular weight in acidic oligosaccharides, were further purified by gel filtration on Bio-Gel P-4 column. Two acidic oligosaccharides (fractions 10-I and 10-II), heptasaccharides, were separated by gel filtration on a Bio-Gel P-4 column from fraction 10. These structures were determined by methylation analyses, nitrous acid deamination after hydrazinolysis and Smith degradation after desialylation. In addition, the structures were also analyzed by direct-probe mass spectrometry of the permethylated derivatives before and after desialylation. These studies indicated that one of them (fraction 10-I) was NeuNGc alpha(2 leads to 3)Gal beta(1 leads to 4)GlcNac beta(1 leads to 3)Gal beta(1 leads to 4)GlcNAc beta (1 leads to 3)Gal beta(1 leads to 3)GalNAcol and another heptasaccharide (fraction 10-II) was Gal beta (1 leads to 4)GlcNAc beta (1 leads to 3)Gal beta (1 leads to 3)[NeuNGc alpha(2 leads to 3)Gal beta(1 leads to 4)GLcNAc beta(1 leads to 6)]GalNAcol. Although another acidic fraction (fraction 13, was obtained as a single peak on a Bio-Gel P-4 column, it appeared to be the mixture of a heptasaccharide, NeuNGc alpha(2 leads to 3)Gal beta(1 leads to 4)GlcNAc beta(1 leads to 3 or 6)[Gal beta (1 leads to 4)GLcNAc beta (1 leads to 6 or 3)]Gal beta (1 leads to 3)GalNAcol and an oligosaccharide similar to fraction 10-II, by analysis of two products obtained by Smith degradation after desialylation.

Animals↗
PubMed1982

Isolation and characterization of alkali-labile oligosaccharide units from porcine erythrocyte glycophorin.

The oligosaccharide units of glycophorin isolated from porcine erythrocyte membranes were released by alkaline borohydride treatment and purified by gel filtration and ion-exchange chromatography. Structures of the O-glycosidic oligosaccharides were determined by methylation analysis, the methylated sugar being identified by gas-liquid chromatography-mass spectrometry and nitrous acid deamination after hydrazinolysis. The major oligosaccharide was a trisaccharide, Gal(1 leads to 3)[Neu-NGly(2 leads to 6)]GalNAc. The other oligosaccharides were larger and contained Glc-NAc. One was a pentasaccharide, Gal(1 leads to 3)Gal(1 leads to 4)GlcNAc(1 leads to 3)Gal(1 leads to 3)GalNAc. The structure of the trisaccharide was also analyzed by direct-probe mass spectrometry of the permethylated derivative, and the result obtained was consistent with the proposed structure.

Animals↗
PubMed1982

Stimulation of migration of human monocytes by bacterial cell walls and muramyl peptides.

Bacterial cell walls, water-soluble fragments of the wall peptidoglycan, N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), and 6-O-acyl derivatives of MDP were examined for migration-stimulating activity on human peripheral blood monocytes by using a multiwell chemotaxis assembly. Cell walls isolated from 11 bacterial species caused a definite increase in monocyte migration, but the walls of Micrococcus lysodeikticus were scarely active. The migration-enhancing activity of Staphylococcus epidermidis cell walls was retained by a monomer as well as a polymer of disaccharide peptides which were prepared by digestion of the peptidoglycan with enzymes. It was finally revealed that the migration of monocytes was enhanced by MDP. 6-O-Octadecanoyl-MDP, 6-O-(2-tetradecylhexadecanoyl)-MDP, and 6-O-(3-hydroxy-2-docosylhexacosanoyl)-N-acetylmuramyl-L-seryl-D-isoglutamine were active, but to a lesser extent. A checkerboard assay demonstrated that the increased monocyte migration caused by S. epidermidis cell walls was directed toward a positive stimulus (chemotaxis).

Acetylmuramyl-Alanyl-Isoglutamine↗
PubMed1982

Nephritic factor (NeF) of alternate pathway (NeFA) and of classical pathway (NeFc).

NeFA and NeFc were studied in various cases (115 cases). 5 cases were followed up for a long time. NeFA assay was done by the "microtest plate method". We detected the NeF activity for the first time in the cases of Sjögren syndrome (SjS) and SjS+SLE+Hashimoto's disease (Hashimoto). In some cases NeF activity disappeared after therapy, and in one case NeFA and NeFc were positive at first, but then only NeFA activity became negative following the adequate therapy. As to the antibody nature of NeF, the possibility was suggested that NeFA might be anti C3b autoantibody and NeFc might be anti C4b and/or C4b2a auto-antibody.

Complement Activation↗
PubMed1982

Binding metal elements in metallothionein fraction from liver of rat injected various metals.

Six elements, Cd, Co, Cu, Mn, Se and Zn, in the metallothionein fractions from liver of rat injected various metals (Cd, Co, Cu, Hg, Se and Zn) were analyzed by neutron activation analysis and were investigated metal binding of it. The concentrations of Cd and Zn were increased by administration of Cd and those of Co and Se were also increased by administration of the each element. On the contrary, the concentration of Cd and Cu were decreased by administration of Se. It may be considered that Co and Se are attached to metallothionein, if its protein is present and the presence of Se influence the binding of Cu and Cd to metallothionein, further it also influence Cd-thionein production.

Animals↗
PubMed1982

[Amyl nitrite test in the evaluation of left ventricular function: application to ischemic heart disease and Duchenne's progressive muscular dystrophy].

The effect of amyl nitrite (AN) inhalation on the left ventricular function was evaluated by mechanocardiography and echocardiography. The patient's group consisted of 110 cases with ischemic heart disease (IHD) and 25 cases of dystrophia musculorum progressiva (DMP) of Duchenne type. The former was a representative of impairment of blood supply and myocardial involvement, and the latter was of predominant myocardial disease. The control was age-matched 32 normals for IHD group and 17 cases for DMP group. Left ventricular function was mainly evaluated by systolic time intervals (STI) and the echocardiographic correlates. Fifty-five cases of IHD group were tested by coronary angiography and left ventriculography and these data were compared with the noninvasive measures. The results were as follows: I. IHD group: The ratio of ejection time (ET) to pre-ejection period (PEP), ET/PEP, did not change so much as in controls after AN inhalation, and this percent change was much smaller in cases with lesions of the left anterior descending artery (LAD) than in cases with lesions of the right coronary artery (RCA). On the other hand, mean posterior wall velocity (mPWV) and posterior wall excursion (PWE) changed greater in patients with LAD lesion than in those with RCA lesion. In cases with LAD stenosis, percent change in ET/PEP was smaller in cases with asynergy than in cases without it, disclosing more significant impairment of the left ventricle in the former. II. DMP group: In severe cases, ET/PEP was small even at rest, and percent change by AN inhalation was smaller than control in mild cases and smallest in severe cases. This seems to be useful in evaluating the severity of the diseased process. The mPWV and PWE showed impairment of left ventricular motion even at rest, but it was clearly showed in severe cases after AN inhalation. These results indicate that impairment of left ventricular function induces the poor response to AN inhalation and this, in turn, results in the lack of hyperactivity of the heart produced by this drug.

Adolescent↗