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Biomedical subjects

K C Bergmann

Publications and source records attributed to K C Bergmann.

At least 91 records · Page 5Linked to original sources
PubMed1982

Occurrence of influenza specific antibodies in the lung after oral immunization in mice.

After nasal, oral and rectal immunization of Balb/c and CF 1 mice with influenza virus (A/PR/8/34, HON 1) specific antibodies (hemagglutination inhibition test) occur in serum and in the lung of mice, detectable in lung lavage fluids. The nasal route of immunization leads to slightly better local antibody production, but the oral route is a promising route, and should be tried in humans.

Administration, Intranasal↗
PubMed1982

Oral immunization against influenza.

An anti influenza vaccine was administered by oral route, as capsules, to 24 volunteers. The presence of IgAs in nasal secretions led us to the fact that some lymphocytes of the small intestine were the precursors of circulating IgAs, and, while proliferating, they settled on mucous sites at a distance.

Administration, Oral↗
PubMed1981

[Results of studies on dogs after unilateral allogeneic lung transplantation without immunosuppression].

The observations and the results after allogenic lung transplantation in 24 receivers were reported. The animals were not treated immuno-suppressively. They survived 6.2 days on an average and died in consequence of the rejection. The transplant is progressively threatened of bacterial infections after the beginning of the rejection even in case of an operation under sterile conditions. The investigations confirmed a narrow correlation between the leucocyte count and the course of the rejection. However, the condition of the transplant can be estimated approximately certainly by means of complex clinical methods of examination.

Animals↗
PubMed1981

[Immunological methods in influenzavirus-infections (author's transl)].

Immunological methods for detection of specific antibodies after viral infections are described. The counterimmunoelectrophoresis detects various precipitating antibodies in serum and sputum (against organic dusts, p.e. bird proteins, aspergillus and viral antigens) and is suitable for routine-work due to its sensitivity and simplicity. The sepharose-fluorescence-test is very sensitive and only small quantities of test reagents are required (detection of IgA-, -M, -G-antibodies). The LN-Test (lectin-neuraminidase-test) is well suitable to detect virus-neuraminidase activities as well as antibodies against neuraminidase in human sera.

Antibodies, Viral↗
PubMed1981

[Bronchoalveolar lavage fluid--a new material in pulmonology].

Bronchoalveolar lavage is a new method of evaluating the inflammatory and immune processes of the human lung. Sufficient material are obtained from normal individuals to allow characterization of cells and proteins. Comparisons with lung biopsies show that these constituents are representative for the composition of the alveolar structures. With the use of lavage new insights will be obtained into the pathogenesis of interstitial and other lung disorders.

Humans↗
PubMed1980

[Allergic alveolitis (pathogenesis and diagnosis)].

Extrinsic allergic alveolitis--a lung disease with low incidence--is caused by antibody and cell-mediated allergic reactions due to inhaled antigens (several organic dusts with fine particle sizes). The diagnosis is based on anamnesis, clinical examination, immunological methods of examination (including detection of antibodies, skin tests, allergen inhalation tests), chest X-ray and lung function tests. The therapy consists in antigen deficiency and corticosteroids.

Adrenal Cortex Hormones↗
PubMed1980

Site and type of impaired lung function in extrinsic allergic alveolitis.

We investigated 109 persons exposed and sensitized against organic antigens. 46 of them (group I) are affected with the typical clinical and roentgenological signs of allergic alveolitis. The other 59 subjects (group II) were sensitized only. Included in all examinations were immunological diagnosis by means of counterimmunoelectrophoresis, systematic interviewing for case histories, and registration of respiratory symptoms. Pulmonary function was recorded by means of the following techniques: analysis of ventilation (flow-volume indices), respiratory mechanics of the larger airways and lung (body plethysmography, esophageal balloon technique), respiratory mechanics of the small airways (closing volume, amplitude of cardiogenic oscillations), distributional analysis, diffusion analysis, blood gas analysis, and right heart catheterization. Extrinsic allergic alveolitis caused changes in lung mechanics, in airways as well as in lung tissue. The most impressive findings in allergic alveolitis are mechanical inequalities of ventilation. These inequalities may be caused by changed mechanics of overall airways indicated by obstruction parameters and by changed structure of lung tissue reflected in decreased compliance, transfer factors and increased pulmonary arterial pressure. Not only a restrictive but also an obstructive disturbance of ventilation is found. Impairments are less in sensitized persons. We find slight mechanical and ventilatory inhomogeneities. An overall airway obstruction is obvious but not to such a large extent as in allergic alveolitis. On the contrary, here the lung tissue is not affected. Compliance and diffusion are within the normal range. There is no typical functional pattern specific for the diagnosis of allergic alveolitis.

Adult↗
PubMed1980

[Effect of smoking on immune function (author's transl)].

Several tobacco antigens have been isolated inducing both precipitating and reaginic antibodies in humans and experimental animals. Chronic smoking leads to histological changes in the respiratory tract. --Alterations of humoral immunity have been demonstrated in the respiratory tract of smokers. Smoking may impair the systemic humoral immunity both in vitro and in vivo. Cell-mediated immunity are altered locally and systematically in smokers. --The clinical incidence of tobacco allergy is not sure, but there are evidences that allergic individuals are more sensitive to the nonspecific noxious effects of active or passive smoking than healthy individuals.

Adult↗
PubMed1980

Simple test for detection of virus neuraminidase and antineuraminidase using lectins (lectin-neuraminidase test system).

The fast and sensitive detection of virus neuraminidase effectiveness (influenza virus A and B or mumps virus) and of antibodies against virus neuraminidase in human serum is described. Lectin mainly obtained from Arachis hypogaea and Helix pomatia is used in the lectin-neuraminidase test (LN-test). The lectins are capable of agglutinating erythrocytes after virus incubation because virus neuraminidase reveals the T-antigen to be situated on erythrocytes. The presence of anti-neuraminidase in human sera can be detected with the aid of lectins after incubation of the virus suspension with human serum as a result of the agglutination having failed to take place. Neuraminidase activities of the influenza virus A and B are detectable up to a virus dilution of 1: 40,000 and anti-neuraminidase could be determined in human serum samples with titres of up to 1: 10,000. All materials required for the LN-test can be arranged in a set.

Animals↗
PubMed1980

[Methodical investigations on the leucocyte-migration-test in capillary technique (author's transl)].

The results of the leucocyte-migration-test in capillar technique have only a relative low validity. The antigen concentrations used in the test influence the level of eliberated inhibition factor directly. Reproducible stimulations of the leucocyte migration was also observed. The antigens have a different toxicity on the leucocytes. All cells was vital after 18 hours in leucocyte cultures without antigens.

Adult↗
PubMed1979

[Immunologic diagnosis].

The development of the immunologic basic research and a deeper insight into the pathogenesis of diseases with changed immune reactivity led to an intensivation of the clinic-immunologic in-vivo- and in-vitro-diagnostics. At the instance of the pulmonary diseases according to the classification of Gell and Coombs the immunologic-diagnostic possibilities and problems are shown. Here is referred to the importance of the use of standardized tests and of the wide replaceability of in-vivo-techniques by in-vitro-methods.

Anti-Glomerular Basement Membrane Disease↗
PubMed1979

[Evaluation of lymphocyte transformation test: relationship between nucleus and cell sizes of PHA-stimulated lymphocyes (author's transl)].

A methodological comparision of evaluation of lymphocyte transformation test is described. Three experts examined smear-slides of cells from five lymphocyte cultures and the corresponding controls in a blind study. In the morphological differentiation of 100 cells per slide in comparison to 1 000 cells per slide it was found a good intraindividually but a unsatisfactory interindividually correlation. 1 000 cells from stimulated and non-stimulated cultures shows the greatest difference for the parameter "total cell size" and the lowest difference for the "nucleus plasma relation" by the planimetrically imeasurement.

Cell Nucleus↗
PubMed1979

[Detection of antibodies against Aspergillus fumigatus. 1st report: making of antigens (author's transl)].

A method of antigen preparation from Aspergillus fumigatus has been described. We received the antigens from medium and mycel used for the detection of precipitins and IgE-antibodies. The antigenic components are demonstrated in the immunoelectrophoresis. By the use of absorption we did not find essential differences between medium antigen and mycel antigen for the detection of precipitins. The medium antigen recommended for diagnostic is easier to produce and, moreover, has advantages for the detection of specific IgE antibodies.

Antibodies, Fungal↗