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Biomedical subjects

K Andoh

Publications and source records attributed to K Andoh.

At least 73 records · Page 4Linked to original sources
PubMed1991

Clinical evaluation of a teleradiology system utilizing personal computers and public telephone line.

Practical usefulness of a teleradiology system using CCD camera, personal computer and telephone line was evaluated in a daily clinical practice. Image quality of this system is diagnostic for the majority of abnormalities on radiological images including plain radiographs. Radiological consultation between hospitals in the same city as well as between distant cities using this moderately priced system was thought to be useful in 90% of cases. Teleradiology using compact systems like ours is expected to be useful in the urban clinical environment as well as in distant areas.

Evaluation Studies as Topic↗
PubMed1991

Tissue factor released from leukemic cells.

Using clotting assay and radioimmunoassay (RIA), tissue factor activity (TFA) and TF related antigen (TFR:AG) were determined in an extracellular culture medium of HL-60 cells. After 12 h incubation, TFA and TFR:AG in the medium with endotoxin (EDX: 1 microgram/ml) reached maximums which were 1.8 and 2.1 times greater than those in the medium without EDX, respectively. In the leukemic cells of 10 patients with acute nonlymphoid leukemia (ANLL), TFR:AG showed a significant correlation with TFA (p less than 0.01). On day 1 of the induction chemotherapy, TFR:AG in the 7 patients with DIC significantly increased to 288.9 +/- 153.1 ng/ml (p less than 0.01), whereas no increase in TFR:AG was recognized in the 3 patients without DIC. These results suggest that TF may be released from leukemic cells into the culture medium or blood stream, and that this may correlate with the development of DIC.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1991

Identification of urinary metabolites in rats treated with p-chloronitrobenzene.

Urinary metabolites in rats treated with p-chloronitrobenzene were identified by gas chromatography-mass spectrometry. A single dose of 100 mg/kg body wt p-chloronitrobenzene was administered intraperitoneally to male Sprague-Dawley rats and urine samples were collected from the 8th to 24th hour after the administration. Urinary metabolites were extracted with diethylether at pH 1.0 and pH 10.0 from urine samples hydrolyzed with acid and base and from intact urine samples. Aliquots of the ethereal extracts were injected into a gas chromatograph-mass spectrometer. Nine substances were identified: p-chloroaniline, 2,4-dichloroaniline, p-nitrothiophenol, 2-chloro-5-nitrophenol, 2-amino-5-chlorophenol, p-chloroformanilide, 4-chloro-2-hydroxyacetanilide, a small amount of p-chloroacetanilide and traces of unchanged p-chloronitrobenzene.

Animals↗
PubMed1991

[The effect of oral administration of roxatidine acetate hydrochloride 3 hours prior to the operation on pH and volume of gastric juice].

The effect of roxatidine acetate hydrochloride, administered 3 hours prior to induction of anesthesia, on pH and volume of gastric juice was investigated in preoperative patients. In fifty patients, who were scheduled to undergo elective surgery, 150 mg of roxatidine acetate hydrochloride was administered orally 3 hours before the induction of anesthesia. The volume and pH of gastric juice were measured immediately after the induction of anesthesia. In 46 patients out of fifty, pH of gastric juice was more than 2.5, and its volume was below 25 ml. In another 4 patients, pH of gastric juice was more than 2.5, or its volume below 25 ml. We conclude that, oral administration of 150 mg roxatidine 3 hours preoperatively could be effective for the prevention of an aspiration pneumonitis.

Administration, Oral↗
PubMed1991

[An X-ray study of catheters abnormally placed in the epidural space].

We investigated the roentgenogram of the epidural catheter in 82 patients in whom the injection of local anesthetics had no effect. Contrast medium 0.5 ml was injected through the epidural catheter and antero-posterior roentgenogram was taken. The roentgenograms were categorized into three patterns, according to the relationship of the catheter to the pedicle of lamina, or to the spread of contrast medium. In 43 cases, the catheter passed through the intervertebral foramen. The tip of the catheter was located outside the foramen, and the contrast medium spread outside the vertebra. In 4 cases, the tip of the catheter was located laterally in the vertebral column, and only the catheter itself was contrasted but no spread of medium was observed. The catheter was thought to be misinserted intravascularly. In other 35 cases, catheter ran laterally on the pedicle of lamina, or the spread of medium 2 mins after the injection was indicated by a dumpling-shape even when the tip was in the vertebral column. In these cases, the catheter was thought not to be outside the epidural space.

Anesthesia, Epidural↗
PubMed1991

T-zone lymphoma in association with systemic lupus erythematosus.

Two patients with systemic lupus erythematosus (SLE) and T-zone lymphoma are described. On admission, both showed arthralgia, generalized lymphadenopathy, hypergammaglobulinemia and positive antinuclear antibody. Lymph node biopsies revealed diffuse infiltration of atypical T-lymphocytes in the expanded interfollicular area (T-zone), a finding characteristic for the T-zone lymphoma. Renal biopsy showed lupus nephritis and neoplastic lymphoid cell infiltration in the glomeruli of one patient, but only diffuse infiltration of neoplastic lymphoid cells and mature plasma cells were observed in the interstitium of the other patient. Both patients responded remarkably well to prednisolone (1 mg/kg/day).

Humans↗
PubMed1991

[A case of pneumothorax in a patient with costal exostosis].

A 21-year-old male was admitted to our hospital complaining of chest pain. He had undergone operations on multiple exostoses of his lower extremities when he was thirteen and fifteen years old. Family history revealed multiple exostoses in his mother and one of his cousins. Chest roentgenograms showed right pneumothorax and a mass arising from the seventh rib in the right lower lung filed. Chest CT revealed that the mass was located in thorax but outside the lung. These findings suggested that the pneumothorax was secondary to the injury of the right lung caused by an exostosis arising from the rib. Upon operation, we found a laceration of the lower lobe and a hard mass the size of a pea protruding from the seventh rib. A small bony spicule was also found to be projecting from the sixth rib. The hard mass and the spicule were resected with a normal portion of the seventh and the sixth ribs. The pathological findings of these bony lesion proved to be consistent with exostosis. The patient's postoperative course was uneventful. This case of pneumothorax caused by an exostosis lacerating the lung is rare.

Adult↗
PubMed1991

The role of endogenous gonadotropin release in the etiology of ovarian enlargement during purified urinary follicle-stimulating hormone therapy.

Patients with polycystic ovarian disease were grouped into three groups according to their maximum ovarian diameter (maxD) after ovulation induction by purified urinary follicle-stimulating hormone (FSH). Serum luteinizing hormone (LH) and FSH were measured daily by radioimmunoassay and size and number of follicles were assessed by ultrasonography. Follicle-stimulating hormone in group A (80 mm less than or equal to maxD) was significantly higher than those of group B (60 mm less than or equal to maxD less than 80 mm) and C (maxD less than 60 mm) for the last 4 days of the treatment. This FSH rise in group A was not accounted for by FSH accumulation by the study of pharmacodynamics, and so was thought to be of endogenous origin. Luteinizing hormone was also elevated 3 days before the administration of human chorionic gonadotropin (hCG) in both groups A and B. The number of follicles in group A at hCG administration was significantly greater than that of group C. Any significant differences were not found in either total amount of purified urinary FSH or in the basal FSH and the LH levels before treatment of the three groups. These results suggest that excessive ovarian enlargement during gonadotropin therapy is caused by multiple follicular development primarily stimulated by endogenous FSH. Endogenous LH release further enhances ovarian enlargement.

Chorionic Gonadotropin↗
PubMed1990

Observations on the cell biology of tissue factor in endothelial cells.

Human umbilical vein endothelial cells (HUVEC) are inducible for tissue factor (TF) activity in culture. Based on experiments using ECGF (4-20 micrograms/ml) with heparin (90 micrograms/ml), we obtained the following results: 1) In confluent HUVEC cultures, ECGF had essentially no influence on the levels of inducible TF. 2) In growing HUVEC cultures, ECGF reduced the TF response shortly after seeding but full response was regained when cells were kept confluent for 2-3 days. 3) Although secondary cultures responded best to TF induction in the absence of ECGF, the response was essentially equal over at least 8 passages in the presence of ECGF. 4) Of total cellular TF induced in HUVEC, about 25% was available on the surface, and less than 4% was released with the shed plasma membrane vesicles. The proportion of total TF activity available on the surface of intact cells was not influenced by the presence of ECGF. 5) T1/2 for the decay of TF activity induced was 8.3-9.5 h, whereas in HUVEC when protein synthesis was blocked after TF induction a T1/2 of about 30 h was found.

Blood Physiological Phenomena↗
PubMed1990

Effect of mannitol on intracranial pressure-volume status and cerebral haemodynamics in brain oedema.

Continuous measurement of CBV and CBF by means of laser-Doppler flowmetry was performed to analyze the temporal profile of cerebrovascular response to water accumulation in an infusion model of brain oedema. Using this method, the effect of mannitol on CBV was also examined to determine how mannitol improves the intracranial pressure-volume relationship. The results show that: (1) The presence of an oedema generator elicits a continuous reduction of CBV and a transient reduction of CBF. With cessation of this mechanical force of oedema production, CBV and CBF return to control levels. (2) Mannitol increases CBV and PVI. The improvement of PVI might be due to an increase in CBV as well as ICP reduction.

Animals↗
PubMed1990

The effects of cerebral haemodynamics on the progression of cold-induced oedema.

A cold-injury lesion was made on the brain of 14 of 21 adult mongrel cats. Cerebral blood flow (CBF) and cerebral blood volume (CBV) were observed in both groups during the next 6 to 9 hours using a laser Doppler flow meter. Intracranial pressure (ICP), water contents, and blood pressure were also measured. Hyperemia frequently reached a peak early in the test period in the gray matter and somewhat later in the white matter. In a few cases, hyperemia was observed to occur late in the test period in the gray matter and even later in the white matter. The least frequent pattern was a continuous decrease of CBF and CBV in gray matter over time and of CBF in white matter. Intracranial pressure rose rapidly and correlated well with the early hyperemia. The water content was significantly increased in the white matter. However, the difference in the rate of the increment of the water content was not significant from 7-9 hours after injury. Hyperemia seems to cause the severe impairment of the microcirculation during oedema formation induced by cold injury. A time difference of hyperemia was detected in the gray matter and the white matter.

Animals↗
PubMed1990

One-stage method for assay of tissue factor activity of leukemic cell with special reference to disseminated intravascular coagulation.

Tissue factor activity (TFA) of leukemic cells (1 x 10(8) cells/mL) was measured in 44 patients with acute nonlymphoid leukemia (ANLL) by the one-stage assay using factor-IX deficient plasma (OSA-dIX) and two-stage assay (TSA). According to the preventative heparin dose schedule based on the TFA measured by the TSA, all disseminated intravascular coagulation (DIC) was controlled successfully. The procedure of the TSA was too complicated for clinical use, and its minimal measurable value was 125 units (U)/L of TFA. The OSA-dIX was simpler in its procedure and sensitive enough to measure accurately a TFA quantity as small as 30 U/L with high reproducibility. In 20 ANLL patients with 125 U/L or more of TFA measured by both assays, there was a significant relationship between their logarithms of TFA (r = 0.93, P less than 0.01). These results suggested that DIC complication in ANLL patients would be controlled successfully by the administration of heparin dosage based on the TFA measured by the OSA-dIX.

Adult↗
PubMed1990

Changes in spinal cord function evaluated by evoked potentials and spinal cord blood flow from a lateral retraction post-cervical laminectomy.

We induced ischemia and stretching by retraction of the spinal cord after laminectomy in 12 anesthetized mongrel adult cats, recorded spinal motor evoked potential (SpMEP) and spinal somatosensory evoked potential (SpSSEP) and determined the spinal cord blood flow (SCBF). The results showed a correlation between progressive deterioration of the function and a decrease in SCBF. Comparing the abnormal findings of both the peak and interpeak latencies in evoked potentials, useful data were more frequently obtained using SpSSEP than SpMEP. Factors involved in their deteriorations seem to be a moderate ischemia by compression, and a stretching by the retraction of the spinal cord. From these results, we conclude that SCBF and SpSSEP are important for monitoring the impulse propagation during retraction of the cervical cord.

Animals↗
PubMed1990

[Changes in arterial oxygen tension during and after enflurane or halothane anesthesia as well as epidural analgesia].

Recovery from inhalation anesthesia is often marked by the occurrence of postoperative hypoxemia. In this study, we compared the effects of enflurane or halothane anesthesia and epidural analgesia on arterial oxygen tension during and after the operation in 60 ASA physical status 1-2 patients who underwent cholecystectomy. Anesthesia was induced with thiopental and maintained with 66% N2O and -enflurane (1.5%), -halothane (1%), or -epidural lidocaine (1% solution, 17.5 ml) in oxygen. Blood gas analysis was done before and 10, 30, 60 min after induction. PaO2 was measured on 1st and 3rd postoperative days in all patients breathing air spontaneously. PaO2 decreased during operation in all three groups of anesthesia. PaO2 values on first postoperative day were significantly lower than those before operation, and PaO2 value in enflurane group (PaO2 = 67 +/- 1 mmHg) was significantly lower than that in halothane group (PaO2 = 72 +/- 2 mmHg, P less than 0.05).

Adult↗
PubMed1990

[Study on the accuracy of uterine endometrial dating].

In order to examine the accuracy of endometrial dating, endometrial dating from 52 infertile women with normal luteal function was investigated. Dating from the histological findings was evaluated from three time points, the onset of the next menstrual period (NMP), the last day of the low phase of basal body temperature (BBT) and the urinary LH peak determined with the "Organon LH Color" kit (LH). Beside microscopic observation, morphometric analysis of endometrial findings was done by means of a high speed color image analysis system "Olympus SP-500". On the day of LH Color (+2), the serum LH level showed a maximum of 35.5 +/- 12.6 mIU/ml (Mean +/- S.D.) (n = 27). The correlation coefficients for histological dating and chronological dating based on NMP, BBT and LH were 0.685, 0.728 and 0.879 in gland and 0.620, 0.737 and 0.833 in stroma, respectively. The multiple correlation coefficient for histological dating evaluated by morphometric analysis and chronological dating based on LH was 0.959. So we conclude that endometrial dating evaluated by morphometric analysis seems to be the most objective and useful.

Adult↗
PubMed1989

Studies on leukemic cell tissue factor.

Apoprotein part of tissue factor of human placenta was purified 871 fold from the starting material with 4.2% yield by concanavalin A-Sepharose affinity chromatography and SDS-PAGE. The molecular weight of purified apoprotein was 45,000 in non-reduced condition and 49,000 in reduced condition. Tissue factor of human leukemia cells (FAB classification:M2 and M3) and cultured leukemia cell lines (HL-60 and Molt-4) was analyzed using specific rabbit anti-tissue factor IgG raised against purified material. Endotoxin stimulated HL-60 and Molt-4 also expressed procoagulant activity which was inhibited by tissue factor immune IgG. By immunostaining of the purified material, the lysate of leukemia cells (M2 and M3) and cultured leukemia cells (HL-60 and MOLT-4) revealed a major band of the same apparent molecular weight. Immuno-electron microscopic study on tissue factor of HL-60 cells produced the following findings: stimulation by endotoxin resulted in the formation of pseudopods of the cell membrane, and immunogold particles accumulated mainly on these pseudopods and cisternal spaces of rough endoplasmic reticulum, indicating exposure of the tissue factor to the surface of perturbed cell membrane with concurrent increase in tissue factor synthesis.

Antibody Formation↗