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Biomedical subjects

J Wasserman

Publications and source records attributed to J Wasserman.

At least 127 records · Page 7Linked to original sources
PubMed1981

Incidence of hypothyroidism after 131I therapy for hyperthyroidism. Relation to pretherapy serum levels of T3, T4 and thyroid antibodies.

A correlation is reported between serum levels of triiodothyronine (S-T3) and thyroxine (S-T4) before treatment, as well as levels of thyroid antibodies before treatment, and the development of hypothyroidism following 131I therapy in 86 patients with hyperthyroidism. Patients with marked elevation of S-T3 or S-T4 had demonstrable antibodies to thyroid cytoplasmic antigen more often than those with normal or moderately elevated levels, and patients with markedly elevated levels of S-T3 also had a higher incidence of hypothyroidism after treatment. Patients with nodular thyroid glands and with markedly elevated levels of S-T3 required a larger number of 131I doses before no signs of hyperthyroidism persisted in comparison to those with moderately elevated levels.

Adult↗
PubMed1981

Adjuvant radiation therapy compared with cyclic chemotherapy in patients with mammary carcinoma. II. Changes of mitogen responses of blood lymphocytes.

Blood lymphocyte reactivity to purified protein derivative of tuberculin (PPD) and phytohaemagglutinin (PHA) was examined in 62 patients with breast carcinoma who received postoperative adjuvant radiation therapy or cyclic chemotherapy with chlorambucil, methotrexate and 5-fluorouracil. Both treatments impaired the immunologic reactivity of blood lymphocytes as measured by PPD and PHA stimulation in vitro. Radiation therapy seemed to cause more profound and protracted suppression of the PPD response than chemotherapy.

Adult↗
PubMed1981

Adjuvant radiation therapy compared with cyclic chemotherapy in patients with mammary carcinoma. I. Changes of blood lymphocyte subpopulations.

Peripheral lymphocyte subpopulations were examined in 37 women with breast carcinoma who were postoperatively randomized to adjuvant local radiation therapy or cyclic treatment with 5-Fluorouracil, Methotrexate and Chlorambucil. Both T and non-T lymphocyte counts were reduced but the latter subpopulation was reduced to the highest relative extent in both groups of patients. Following radiation therapy the non-T cells repopulated more rapidly than the T cells, whereas the repopulation seemed to be the reverse in patients treated with chemotherapy.

Adult↗
PubMed1981

Blood lymphocyte subpopulations and antibody dependent, cell-mediated cytotoxicity (ADCC) in alopecia areata and universalis.

T-lymphocyte subpopulations and antibody-dependent, cell-mediated cytotoxicity (ADCC) were studied in patients with alopecia areata and universalis. The proportions of T-cells with the receptors for IgG (Tg) and T-cells with receptor for IgM (Tm) were determined in 16 cases. The ADCC of unfractionated lymphocytes was examined in 5 cases. A higher proportion of Tg-cells (T-cell with low affinity for sheep red cells) and a more pronounced ADCC of the peripheral lymphocytes were found in patients with alopecia areata and universalis than in age- and sex-matched normal donors.

Adult↗
PubMed1981

Cell mediated cross-reactivity in vivo and in vitro to purified dermatophyte antigen preparations in sensitized guinea pigs.

Guinea pigs were sensitized to either Trichophyton rubrum, Trichophyton mentagrophytes, or Epidermophyton floccosum. Cross-reactivity experiments were performed in vivo and in vitro with purified antigen preparations prepared from the respective dermatophyte according to the ethylene glycol method. All sensitized animals responded with delayed-type skin reactions to all three antigen preparations, although the strongest reactivity observed was to the homologous antigen. Spleen and blood lymphocytes from many (but not all) animals were stimulated by these antigens and the response to the homologous antigen was always more pronounced. The findings indicate the presence of a common group-specific dermatophyte antigen as well as the possibility of species-specific antigens in the experimental system studied.

Animals↗
PubMed1981

Spontaneous cell-mediated cytotoxicity (SCMC) in patients with alopecia universalis.

The spontaneous cell-mediated cytotoxicity (SCMC) of unfractionated lymphocytes was determined in 5 patients with alopecia universalis (AU). The target cells used were K562 and Chang cell lines. All patients had an increased SCMC as compared with age- and sex-matched donors. The results indicate that SCMC against K562 cells is more pronounced than that against Chang cells.

Adult↗
PubMed1981

ELISA-determined serological reactivity against purified trichophytin in dermatophytosis.

Serological activity in 62 patients wih dermatophytosis was investigated using purified trichophytin preparation as antigen (produced by the ethylene glycol method). Antibodies of IgG and IgM classes were determined by means of ef enzyme linked immunosorbent assay (ELISA). In comparison with 79 controls consisting of groups of healthy blood donors and dermatological outpatients, the dermatophyte-infected patients showed a significantly higher mean IgG response to trichophytin antigen preparation. On the other hand several individual cases were low responders. In a group of 10 control children, no IgG activity against trichophytin could be demonstrated. IgM class antibodies showed no significant differences between the groups investigated. These findings indicate that differences in the amounts of antibodies of IgG class against trichophytin as measured by the ELISA method reflect differences in the degree of mycotic sensitization between groups of individuals having experienced varying degrees of exposure to dermatophytes. Nevertheless, the determination of IgG antibodies in dermatophytosis seems to be of limited clinical value.

Antibody Formation↗
PubMed1981

Blood lymphocyte subpopulations in Mycosis fungoides and their functions in vitro.

T-lymphocyte subpopulations, PWM stimulated in vitro Ig production by mononuclear cells, and spontaneous cell-mediated cytotoxicity (SCMC) were determined in patients with Mycosis fungoides (MF) as well as in sex- and age-matched normal controls. We were able to confirm our earlier findings of a significantly higher frequency of T-lymphocytes with Fc-receptors for IgG (Tg cells) and a proportion of T lymphocytes with Fe receptors for IgM (Tm cells) not significantly differing from normal controls. As defined by monoclonal antibodies, the proportion of T-helper cells (Leu3A) was significantly lower, whereas the T-suppressors cells (Leu2A) were in the range of normal controls. The PWM stimulated and spontaneous Ig synthesis in vitro was lower in MF patients whereas there was no difference in the SCMC activity, in comparison with normal controls. The lower frequency of T-helper cells in the peripheral blood may be explained by their migration to the skin.

Aged↗
PubMed1980

Immunosuppression and alterations of T-lymphocyte subpopulations after rubella vaccination.

Lymphocyte stimulation tests and determinations of T-lymphocyte subpopulations were undertaken in 11 subjects before and after rubella vaccination and in 11 controls. The lymphocyte stimulation response to phytohemagglutinin was suppressed 7 to 10 days after vaccination. The proportion of T lymphocytes with receptors for the Fc part of immunoglobulin G increased after vaccination, whereas there was no significant change in the proportion of T lymphocytes with immunoglobulin M-Fc receptors.

Adolescent↗
PubMed1980

Natural killer activity in peripheral lymphocyte population following local radiation therapy.

The natural killer (NK) activity of peripheral lymphocytes against 51Cr-labelled Chang or K562 cells was tested using a 4 h release assay before and after postoperative radiation therapy for mammary carcinoma. NK activity against K562 was significantly reduced at completion of therapy (a total target dose of 45.0 Gy) and restituted 3 to 4 months later. NK activity against Change cells exhibited a slight but non-significant decline at completion of therapy followed by an overshoot 3 to 4 months later. The frequency of Fc-IgG receptor bearing lymphocytes was decreased at completion of therapy and largely restored after 3 to 4 months.

Adult↗
PubMed1980

In vitro lymphocyte reactivity to rubella antigen following vaccination.

In vitro lymphocyte stimulation and several serological tests were used to study the development of immunity after subcutaneous immunization with attenuated rubella virus. Maximal lymphocyte reactivity was obtained 3--4 months after vaccination and a decline after 1.5--2.5 years. Lymphocyte reactivity following natural immunity was of a higher magnitude. No correlation between the degree of lymphocyte stimulation and titers of neutralizing, hemagglutination inhibiting and hemolysis-in-gel antibodies was observed. The influence on the results of some methodological factors and different rubella antigens is discussed.

Adolescent↗
PubMed1979

Lymphocyte and granulocyte reactions during sleep deprivation.

The possible influence of 48 hr of sleep deprivation on in vitro DNA synthesis of blood lymphocytes and on the adhesiveness and intracellular, stainable activity of alkaline phosphatase in blood granulocytes was studied in twelve young male volunteers. Following the sleep deprivation, all 12 subjects showed marked reductions of DNA synthesis after stimulation with phytohemagglutinin. Pre-exposure levels were regained 5 days after terminating the vigil. No changes were noted in granulocyte adherence or alkaline phosphatase activity. The results suggest that sleep deprivation may decrease cell-mediated immune reactions and thereby impair some aspects of host defense.

Adult↗