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J Testart

Publications and source records attributed to J Testart.

At least 217 records · Page 12Linked to original sources
PubMed1983

A study of factors affecting the success of human fertilization in vitro. II. Influence of semen quality and oocyte maturity on fertilization and cleavage.

The incidence of in vitro fertilization was analyzed with respect to the degree of cumulus dissociation (expansion) at the time of oocyte recovery and also the semen quality. Of the oocytes surrounded by perfectly ("++") or moderately ("+") dissociated cumuli, 78.6% and 30.8%, respectively (P less than 0.001), were fertilized when the husband's semen analysis was in the normal range. The proportion of fertilized oocytes was not decreased in cases of polyzoospermia (greater than 130 X 10(6) spermatozoa/ml), but was decreased (P less than 0.05) when the semen analysis revealed other anomalies: oligozoospermia (less than 15 X 10(6) spermatozoa/ml), asthenozoospermia (less than 50% motile cells) or teratozoospermia (greater than 50% abnormal spermatozoa). The proportion of fertilized eggs cleaving in vitro was unaffected by semen quality but was lower when "+" cumulus oocytes were collected than when "++" cumulus oocytes were obtained (58.3% vs. 87.0%, P less than 0.02). In vitro incubation of the oocyte prior to insemination increased the incidence of fertilization by about 28% for both "+" (22.2 to 50.0%) and "++" (65.7 to 93.9%) cumulus oocytes. Finally, 67.6% of "++" cumulus oocytes developed into embryos when the insemination with spermatozoa from normal semen samples was delayed by several hours, compared with only 29.0% when the conditions were suboptimal ("+" cumulus oocyte, abnormal semen analysis or no delay prior to insemination). Eight pregnancies began following the replacement of 38 embryos in 34 patients. Six spontaneous abortions occurred, and chromosomal abnormalities were proven in the two cases analyzed. Two pregnancies continued for more than 3 months, resulting in term deliveries of two normal babies.

Cell Division↗
PubMed1983

In-vitro ovulation of rabbit ovarian follicles isolated after the endogenous gonadotrophin surge.

Follicular rupture was never observed when preovulatory rabbit follicles were isolated from the ovary before the ovulatory gonadotrophin discharge and placed in hormone-free culture for 10-14 h (n = 48). However, if the follicles were taken 1 h post coitum and cultured under the same conditions, 11/24 (45.8%) ruptured spontaneously. Follicular rupture under these conditions appeared to be chronologically and histologically comparable to that of ovulation in vivo. Culture in the presence of progesterone increased the proportion of follicles ovulating in vitro (17/23 = 73.9%; P less than 0.05). Inhibition in vitro of the synthesis of steroids or prostaglandins suppressed ovulation, although meiotic maturation of the oocyte did take place. These findings indicate that the follicle constitutes an independent entity within the ovary from 1 h post coitum.

Animals↗
PubMed1983

Role of the extra-follicular compartment in the ovulation of isolated rabbit ovarian follicles.

Ovulation was never observed, even in the presence of gonadotrophins, when preovulatory follicles isolated from oestrous does were cultured in vitro (n = 112). If the follicles were co-cultured with pieces of ovarian tissue and gonadotrophins, then 50% ovulated (P less than 0.01). Attempts to replace the ovarian tissue by various steroid hormones or prostaglandins did not result in follicular rupture except when PGE-2 was used (23.8%, P less than 0.05). We suggest that gonadotrophins have an immediate (less than 1 h) action upon the follicle and ovarian interstitial tissue simultaneously. The response of the ovarian tissue to gonadotrophins is essential for eventual follicular rupture and involves PGE-2.

Androstenedione↗
PubMed1983

[Plasma and intrafollicular hormone profiles in the late preovulatory phase. I. Spontaneous cycles].

The hormone state of development in the late preovulatory phase is described in its relationship to the start of the LH surge which occurs 37-39 hours before ovulation. No precise relationship has been found between the oestradiol (E2) peak in the plasma and that which occurs before ovulation. All the same, the succession of hormone events that have been observed in 77 spontaneous cycles is the following: the level of E2 suddenly rises between 35 and 30 hours before the ovulatory discharge and this rise is immediately followed by a rise in the level of LH. Then the plasma concentration of 17-hydroxyprogesterone (17-OHP) increases 5 hours before the LH surge. In the preovulatory follicle 5 hours after the start of the LH surge, a drop in the level of E2 and a rise in the level of progesterone is noted. Concentrations of androgens only lessen in the last 12 hours before ovulation whereas the level of 17-OHP stays stable. We have thus been able to work out the sequence of plasma hormone phenomena that occur in determining whether a preovulatory gonadotrophic surge will occur. We have described the hormone levels in follicular fluid immediately before spontaneous ovulation. These findings will be able to serve as a reference to evaluate the quality of the late preovulatory phase in abnormal cycles or in cycles that have been induced.

Adult↗
PubMed1983

[Treatment of acid gastroesophageal reflux by posterior hemifundoplication. Clinical and pH-metric results].

The efficiency of posterior hemifundoplication (Toupet's procedure) in the treatment of gastroesophageal reflux was studied prospectively in 25 patients. Surgery was performed because of resistance to medical therapy and/or ulcerated esophagitis (12 cases). Clinical symptoms were evaluated three times: preoperatively, from 2 to 4 months after surgery and thirdly at a late post-operative interview (mean 21 months). Esophageal pH was recarded through a 3-h period after a standard meal and acid peptic reflux was expressed using a scoring index taking into account the duration and magnitude of pH fall. Esophageal pH was assessed in all the patients before and shortly after surgery and in 10 patients at the late postoperative control. Twenty-four of the 25 patients, had a dramatic improvement or were symptom-free, from 2 to 4 months after operation; the gastroesophageal reflux score decreased in all but 1 patient, but returned to physiological values in only 19 patients (group A), and remained above the normal range in 6 patients (group B). In the latter subjects, the preoperative score was significantly higher and the time elapsed below pH 3 significantly longer than those in group A. At the late postoperative control, clinical symptoms of gastroesophageal reflux were noted in 10 of the 23 patients interviewed. The incidence of late postoperative deterioration was more frequent in group B patients (4 among 6 subjects) than in group A (6 out of 17), and in patients presenting with severe esophagitis before surgery. In conclusion, while they confirm the immediate clinical efficiency of posterior hemifundoplication in gastroesophageal reflux, these results indicate that an abnormal esophageal pH test persists in 25 p. 100 of patients, and seems to carry an increased risk of further clinical deterioration.

Adult↗
PubMed1983

Treatment of flail chest with Judet's struts.

This report describes our experience with the operative stabilization of flail chest with the use of Judet's struts. In a series of 18 patients with flail chest, the method allowed shorter duration of artificial ventilation and decreased functional sequelae. We find this technique to be better than previously published methods, since it provides better stabilization and immobilization of the ribs and thus obviates the need for artificial ventilation.

Adult↗
PubMed1982

Minimum time lapse between luteinizing hormone surge or human chorionic gonadotropin administration and follicular rupture.

Occurrence of ovulation was detected by laparoscopy between 22 and 47 hours following the onset of the luteinizing hormone (LH) surge in plasma (61 patients) or human chorionic gonadotropin (hCG) administration (76 patients). None of 22 patients had ovulated before the 34th hour following LH surge or hCG, as compared with 3.4% (3 of 89) and 50.0% (11 of 22) laparoscoped after 34 to 37 hours or 37 to 39 hours, respectively. Whether measured with respect to the initial rise of LH or the injection of hCG, the time lapse before ovulation was comparable. Ovulation was more frequently established at 37 to 39 hours in spontaneous cycles (10/13) than in clomiphene-treated cycles (1/9, P less than 0.01). The onset of the LH rise was found to be a more accurate criterion than the LH peak in determining the time of ovulation.

Adult↗
PubMed1982

Hormonal and histological study of the luteal phase in women following aspiration of the preovulatory follicle.

A hormonal and histologic study of 60 luteal phases was carried out in 40 patients during spontaneous and stimulated cycles before and after follicle puncture for oocyte recovery. The duration of the luteal phase was modified neither by follicle rupture nor by hormonal stimulation. The aspiration of a spontaneous preovulatory follicle caused a temporary deficiency in plasma progesterone (P) (P less than 0.01) on the third day following aspiration, and a higher prolactin (PRL) level (P less than 0.02) on the ninth day. Dystrophia of the endometrium was observed in one-third of the cases, whereas the P level was normal. In cycles stimulated by clomiphene citrate, no P deficiency was observed. When luteinizing hormone (LH) discharge occurred spontaneously, biopsies carried out 3 days following follicle aspiration indicated a normal secretory state of the endometrium, and the P level was higher from, the sixth day following aspiration (P less than 0.05), as compared with the control cycles. This phenomenon was not observed when human chorionic gonadotropin (hCG) was administered.

Androstenedione↗
PubMed1982

[Electroencephalographic surveillance during carotid endarterectomy].

Continuous EEG monitoring during 110 carotid endarterectomies showed transient or permanent abnormalities in 35 cases. In 13 cases, these abnormalities occurred during carotid clamping and led the surgeon to place a shunt in 8 cases. In 22 cases, electrical abnormalities of various types appeared outside the clamping period. Continuous EEG monitoring demonstrated that cerebral disturbances can occur at any time during the operative period. Thus, intra-operative EEG monitoring allows a better understanding of clinical signs which may be seen postoperatively.

Anesthesia, General↗
PubMed1982

Premature ovulation after ovarian ultrasonography.

Whereas follicle rupture never occurred before the 37th hour after an ovulatory stimulus (either the onset of the LH surge or hCG administration) in control patients, ovulation was observed at 26 to 36 h in women submitted to ultrasonography during the late follicular phase. Premature ovulation was observed in 5 out of 23 and 8 out of 19 cycles when ultrasonography occurred during the 3 days preceding or in the 36 h following ovulatory stimulus. This as yet unexplained observation leads us to reconsider the advisability of ovarian scan during the late follicular phase of the menstrual cycle.

Adult↗
PubMed1982

Seasonal influence of diurnal rhythms in the onset of the plasma luteinizing hormone surge in women.

The time of the LH surge onset in plasma was detected in 75 human cycles. The preovulatory LH surge began between midnight and 0730 h in two thirds of the patients. According to the season of study, the LH surge began between 1030 and 2215 h in 52.4% of the spring cycles compared with 12.9% of the cycles studied during other seasons (P less than 0.001). The highest frequency of the LH surge occurrence over a 3-h time interval was 1500 +/- 1.5 h during the spring cycles (28.6%) and 0300 +/- 1.5 h (42.5%) during the other seasons. It was estimated that ovulation occurred primarily in the morning during the spring and primarily in the evening during autumn and winter.

Adult↗