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Biomedical subjects

J Reina

Publications and source records attributed to J Reina.

At least 109 records · Page 6Linked to original sources
PubMed1990

Analysis of the autoagglutination phenomenon, serogroup and hydrophobicity as virulence markers in mesophilic aeromonads isolated from stool cultures.

The autoagglutination phenomenon (AA) was studied in 71 strains of mesophilic aeromonads isolated from stool cultures of patients with acute diarrhea (28 Aeromonas hydrophila, 20 Aeromonas sobria, and 23 Aeromonas caviae). Two phenotypes were considered in the AA+ strains: SP+ (self-pelleting) and PAB+ (precipitation after boiling). Serogrouping of the strains was performed only with the 0:11 serum. Other properties studied were agglutination with acriflavine (Acr) and crystal violet uptake (CVB). Phenotype AA+ was found in 29.5% of the strains; 7% of these AA+ strains exhibited the SP+ PAB+ pattern and 22.5% the SP- PAB+ pattern. The species Aeromonas hydrophila comprised the highest proportion (28.5%), and Aeromonas caviae the lowest (13%). Fifty percent of the PAB+ strains of Aeromonas hydrophila and Aeromonas sobria belonged to serotype 0:11. None of the PAB+ strains of Aeromonas caviae belonged to this serotype. 8.4% of the strains were Acr+ and 23.9% CVB+.

Acute Disease↗
PubMed1990

Microbiological characteristics of Weeksella virosa (formerly CDC group IIf) isolated from the human genitourinary tract.

Weeksella virosa (formerly CDC group IIf) is a nonsaccharolytic, oxidase- and catalase-positive, gram-negative rod which is unable to grow on MacConkey agar. At 48 h of incubation on blood or chocolate agar, the colonies present a characteristic appearance: intensely mucoid, adherent, and cream colored as a result of the production of a nondiffusible yellow pigment. This microorganism has been isolated predominantly from the female genitourinary tract, which indicates the opportunity for sexual transmission. We present the microbiological study of three strains of W. virosa identified in a study of 707 female genital samples, representing an incidence of 0.42%. At the same time, we analyzed the principal biochemical tests used in the identification of this microorganism and the susceptibilities of the organism to the different antimicrobial agents assayed.

Female↗
PubMed1990

[Biochemical, physiological, and enzymatic study of 78 strains of Helicobacter (Campylobacter) pylori isolated from gastroduodenal biopsies].

A biochemical, physiological and enzymatic study of 78 C. pylori strains isolated from gastroduodenal biopsies is reported. All strains were positive in the oxidase, catalase and urease tests. 97.4% produced SH2 in the lead acetate band and 79.4% showed beta-hemolytic activity in sheep blood agar. In the antibiotic selection tests, C. pylori was characterized to be resistant to nalidixic acid and sensitive to cefalotin . The enzymatic study demonstrated the presence of acid and alkaline phosphatases. This finding and the urease test give C. pylori a define bacteriological character which differentiate it from the remaining campylobacteria.

Anti-Bacterial Agents↗
PubMed1990

[Analysis of the sensitivity of mesophilic Aeromonas isolated from feces to antibiotics of everyday use].

The sensitivity to antibiotics of 56 mesophilic strains of Aeromonas (20 A. caviae, 18 A. sobria and 18 A. hydrophila) isolated from the feces of patients with enteritis, 89.2% of strains had some type of resistance, such as 83.9% to ampicillin, 32.1% to carbenicillin, 28.8% to cefalotin, 5.3% to co-trimoxazole and 1.7% to chloramphenicol. 48.2% of strains were simultaneously resistant to two of the tested antibiotics, 3.5% to three and 5.3% to four. 95.7% of ampicillin resistant strains were beta-lactamase producing. A. sobria was more sensitive to cefalotin (MIC less than 8 micrograms/ml) than the remaining species. This finding supports the use of that marker for identification.

Acute Disease↗
PubMed1989

[Chronic fatigue syndrome associated with Epstein-Barr virus infection].

Epstein-Barr virus (EBV) infection is ubiquitous and may result in multiple and widely different clinical features; the most common of these is infectious mononucleosis (IM). Recently, a group of patients has been included in the chronic EBV infection syndrome (EBVIS), with a sustained nonspecific syndrome consisting of asthenia, anorexia, low grade fever and changes in mood, associated with a viral infection not necessarily caused by EBV; this has been called chronic fatigue syndrome (CFS). We report a patient who fulfilled the criteria for CFS associated with EBV after an acute, well documented EBV infection. We discuss its etiological and pathophysiological implications, emphasizing the need for extreme caution in the diagnosis of CFS. A merely clinical diagnosis may hide severe mistakes.

Adult↗
PubMed1989

Urinary tract infection caused by Enterobacter taylorae.

We present a case of urinary tract infection caused by Enterobacter taylorae in a 70-year-old male with renal lithiasis. The microorganism was isolated in significant numbers from a urine culture. The disappearance of clinical symptoms after antibiotic treatment points to the participation of this microorganism as the etiological agent in the infection.

Aged↗
PubMed1989

[Campylobacter pylori's sensitivity to the bactericidal action of human serum].

The sensitivity to the bactericidal power of the human serum was evaluated on 8 strains of C. pylori, 8 strains of C. jejuni, 2 strains of C. coli and 2 strains of C. fetus. Each of them was incubated at 37 degrees C in the presence of a complete pool of human sera, the same pool after inactivation of the complement, and trypticase soy broth (control). After 0, 15, 30 and 60 minutes the capacity to grow in blood agar plates (count of colonies) incubated in microaerophillic conditions for 3 days was evaluated. In this experimental study it was shown that C. pylori has a rapid and complete sensitivity to the human serum and, particularly, to the complement; this could be one of the factors that determine its lack of invasive capacity.

Blood Bactericidal Activity↗
PubMed1989

[Effectiveness of ClNa-esculin broth for the rapid identification of the Enterococcus genus].

The efficacy of the ClNa-esculine broth for the rapid identification (2 hours) of the species of the genus Enterococcus was evaluated and compared with the conventional biochemical methods requiring an incubation of 18-24 hours. Out of the 274 evaluated strains, 171 were positive for this investigation; 160 of them corresponded to Enterococcus spp (100%). The sensitivity of the test was 100%, the specificity 99.1%, the positive predictive value 99.3% and the negative predictive value 100%.

Culture Media↗
PubMed1989

[Human infections caused by Enterobacter sakazakii. Microbiologic considerations].

The clinical and microbiological features of four patients with positive cultures for Enterobacter sakazaii are reported. They corresponded to two wound exudates, one appendicitis and one newborn conjunctivitis. The biochemical and physiological characteristics of this organism are analyzed (inability to ferment d-sorbitol and production of a non-diffusible yellow pigment); its difficult identification and the clinical rarity of its isolation are discussed. The relevant literature is reviewed.

Adult↗