Sphingobacterium multivorum isolated from a patient with cystic fibrosis.
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Biomedical subjects
Publications and source records attributed to J Reina.
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An unusual case of chronic intussusception, without any digestive sign, secondary to mesenteric lymphadenitis caused by Yersinia enterocolitica is reported. Operative reduction by taxis was performed but ileopexy and antibiotic treatment were also carried out to reduce chances of recurrent intussusception.
We present a case of a 50-year-old woman who was admitted to the hospital as a result of a process affecting the lower respiratory tract, associated with a community-acquired bacteremia due to Serratia plymuthica. The patient was treated with erythromycin and gentamicin and was discharged to home 7 days after admission with complete clinical cure. In view of the lack of reports concerning human infections by S. plymuthica, we present our case and review the literature concerning the microbiological and clinical characteristics of this unusual human pathogen.
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We study the "suicide" phenomena as well as metabolic pathways of mixed acids (methyl red test, MR) and butylene glycol (Voges-Proskauer, VP), in 107 strains belonging to mesophilic Aeromonas group, isolated from stools. The strains have been identified as A. hydrophila, 28 cases (26.1%), A. sobria 26 cases (24.3%) and A. caviae 53 cases (49.6%). All A. caviae strains behave homogeneously as F+, RM+ and VP-, independently of temperature (30 or 37 degrees C). A. hydrophila strains only showed their trend to "suicide" at 37 degrees C, being this behavior linked to RM test positivity. At 30 degrees C all strains were NS and RM-, keeping always positive the VP test (both temperatures). In A. sobria we have recorded changes in their behavior related to the temperature of incubation. At 37 degrees C, 57.7% were NS, whereas at 30 degrees C, 69.2% showed the same phenotype. The metabolic activity had remained stable, therefore F+ strains were VP and RM+, and NS strains were VP+ and RM-. It seems that FS is a phenotypic behavior of this bacterial group species and temperature-dependent, and also is related to a fermentative metabolic activity modulation of each of them.
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We have studied the restricted antibiotic susceptibility tests for 180 strains belonging to mesophilic Aeromonas sp. isolated from stools samples. Strains has been identified as follows: A. caviae (64.6%), A. hydrophila (17.7%) and A. sobria (17.7%). Antibiotics tested were ampicillin (10 micrograms), carbenicillin (100 micrograms), cephalotin (30 micrograms) and colistin (10 micrograms), by means of agar diffusion tests (disk-plate). We identified four different patterns of antibiotic susceptibility. The most frequent pattern (pattern #1) (77.8%) is characterized by susceptibility only to colistin, and was predominant among A. caviae strains (80%). Pattern #2 (susceptibility to cephalotin and colistin) was found in 18.9% of strains, being predominant in A. sobria (88.2%). We confirm the higher susceptibility of A. sobria sp. to cephalotin as well as the possibility that these different patterns might be used as screening method for species identification among mesophilic group of Aeromonas.
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We evaluate the presence of heat-stable STa toxin producers E. coli strains from stools of patients with acute diarrhoea. As detection method, we used a commercially-available enzyme-linked competitive assay (ELISA). Over a three months period, 279 E. coli strains were analyzed. Only two (0.71%) were STa toxin producers. Both isolates are from pediatric cases (2 and 4 years of age), without any epidemiologic relationship between them. Therefore, we conclude that this infection could be considered as a non-epidemic community-acquired diarrhoeal disease.
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Phenotypic characteristics of 100 strains pertaining to the group of mesophilic aeromonas isolated in feces of patients with diarrhea (23 A. hydrophila, 34 A. sobria, 19 A. caviae, and 24 considered atypical because produced a the negative esculin reaction and a positive gas formation from glucose [TSI]). The percentages obtained in the different biochemical tests support the hypothesis that in this group there is a taxonomic complexity. We observed variations in the following tests: LDC, arabinose, Voges-Proskauser, lactose, and motility and hemolytic activity. We compared manual and automatic procedures in detecting esculinase and beta-galactosidase activity (ONPG). The study of constitutional enzymatic activity by means of API ZYM system can not be used to differentiate the distinct species although the enzyme beta-glucosidase is detected preferentially in A. hydrophila.