Search PubMed⌕ Search

Biomedical subjects

J Murray

Publications and source records attributed to J Murray.

At least 361 records · Page 20Linked to original sources
PubMed1984

Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor.

A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.

Angiogenesis Inducing Agents↗
PubMed1984

Late metastases of ovarian carcinoma. A case report.

In cases of ovarian carcinoma distant metastases are rarely discovered before local spread has become evident. This article reports an unusual case in which renal metastases appeared 9 years after the initial diagnosis of epithelial ovarian carcinoma. A discussion of the histological features of the tumour and the spread of ovarian carcinoma is included.

Adult↗
PubMed1984

Controlled release of microquantities of macromolecules.

A technique for insuring the controlled release of small amounts of macromolecules such as polypeptides from polymeric delivery systems is described. We show that incorporation of albumin in milligram quantities into these delivery systems can facilitate the sustained release of nanogram or microgram quantities of a model macromolecule such as inulin. The albumin-containing controlled-release polymers did not cause inflammation or tissue damage in two commonly used assay sites for certain biological growth factors such as tumor angiogenesis stimulators and inhibitors--the chick chorioallantoic membrane (CAM) and the rabbit cornea. The method reported here should be particularly suited to the delivery of purified growth factors which are active and usually obtainable in microgram or smaller amounts.

Animals↗
PubMed1984

Prolonged skin photosensitization induced by methoxsalen and subphototoxic UVA irradiation.

Topical 8-methoxypsoralen (8-MOP) was used to briefly provide free psoralen sufficient for marked cutaneous photosensitization, but only a small dose of UVA was delivered initially, in an effort to produce many monoadducts but few crosslinks. After ample time for clearance of the remaining free psoralen a second UVA exposure was delivered. The second exposure should not have generated any additional monoadducts in the absence of free psoralen, but the remaining monoadducts could be converted to crosslinks. The observation of a prolonged persistent UVA-photosensitive state caused by prior, very small doses of UVA given while free 8-MOP was present strongly suggests that psoralen-DNA crosslinks per se initiate much of the phototoxic effect of 8-MOP on skin, and that monoadducts induce much less acute inflammatory response. Because erythema was studied as the end point, the data say nothing about relative contributions of monoadducts vs crosslinks in causing mutagenesis, hyperpigmentation, therapeutic or other cutaneous responses. Other explanations for the induced persistent photosensitive state are also possible, but less tenable or entirely hypothetical.

DNA↗
PubMed1983

Osteosarcoma: intra-arterial treatment of the primary tumor with cis-diammine-dichloroplatinum II (CDP). Angiographic, pathologic, and pharmacologic studies.

Intra-arterial CDP was utilized to treat the primary tumor in 11 pediatric patients with osteosarcoma and in one with malignant fibrous histiocytoma. The investigation commenced with a phase I-II pilot study in four osteosarcoma patients. A dose of 150 mg/m2 was found to be safe and effective in producing a clinical response. This was followed by a definitive study in the remaining seven osteosarcoma patients and in the one malignant fibrous histiocytoma patient. The results were assessed by specific clinical, pharmacologic, radiographic and pathologic criteria. The overall response in the definitive study was 50% with two patients exhibiting total tumor destruction. The success of intra-arterial CDP was attributed to its ability to achieve high local drug concentration and tumor penetration. This was demonstrated by pharmacologic studies.

Adolescent↗
PubMed1983

Angiogenesis inhibitors and their delivery systems.

The extensive vascularity of solid tumors has been recognized for over 100 yr (1). However, only in the past 15 yr has the importance of this phenomenon been appreciated and only in the past 6 yr has the possibility of chemical interference been apparent. In this article, we review the emerging field of tumor vascularization inhibitors.

Angiogenesis Inhibitors↗
PubMed1983

Spielberger's State-Trait Anxiety Inventory: Measuring anxiety with and without an audience during performance on a stabilometer.

Half of the 56 subjects (n = 28) performed 15 pre-treatment trials on a stabilometer, then six more with an audience of three faculty (Group 1), the other half performed the same task with no audience (Group 2). Subjects completed the State-Trait Anxiety Inventory prior to and after the treatments. Orthogonal contrasts indicated that Group 1 (audience) post State-anxiety was significantly different from its own pre-State-anxiety and significantly different from Group 2 (no audience) on post-State-anxiety. It was concluded that the State-Trait Anxiety Inventory is an appropriate measure of trait and state anxiety in studies of motor performance.

Adolescent↗
PubMed1983

Restriction endonuclease mapping of gamma-delta-beta-globin region in G gamma (beta)+ HPFH and a Chinese A gamma HPFH variant.

Restriction endonuclease mapping of the beta-globin genomic region was used for studying the molecular basis of two variants of hereditary persistence of fetal hemoglobin (HPFH): an African G gamma (beta)+ HPFH and a Chinese HPFH variant with predominant synthesis of A gamma chains. HPFH and control DNA samples were digested with a battery of restriction enzymes, and the fragments were identified by hybridization to a family of discrete probes. DNA fragments from the A gamma HPFH (Chinese) and the G gamma (beta)+ HPFH individuals were identical with those of the normal controls. These findings suggest that the two mutants are the result of small structural anomalies of DNA sequences that play a role in the regulation of the expression of gamma-globin genes.

Chromosome Mapping↗