Search PubMed⌕ Search

Biomedical subjects

I Kondo

Publications and source records attributed to I Kondo.

At least 307 records · Page 17Linked to original sources
PubMed1977

A bacteriological study on children with staphylococcal toxic epidermal necrolysis in Japan.

From 1973 to 1976, 21 patients with staphylococcal toxic epidermal necrolysis were admitted to the Jikei University Hospital in Tokyo. Of all of them were isolated coagulase-positive Staphylococcus aureus from various sites. In Japan, unlike in Europe and America, phage group II cocci were found in few cases. On the other hand, S. aureus belonging to other phage groups than group II (I, III mixed group and III group) were very commonly isolated. All the isolates produced exfoliatin which was capable of producing exfoliation in newborn mice. The type (A or B) of exfoliation which was detected by the antiserum agar method did not correlate to the severity of this disease.

Child↗
PubMed1977

Mercury resistance and R plasmids in clinical isolates of Kebsiella pneumoniae.

The mercury and antibiotic resistance of 155 clinical isolates of Klebsiella pneumoniae (K. pneumoniae) was determined. Resistance to mercury tested was found in 64.5% of the total isolates. The frequencies of resistance to SM, TC, CP, KM and GM were 65.8%, 49.0%, 48.4%, 37.4% and 3.9%, respectively. And we selected 94 mercury and drug resistant strains, and they were tested for their ability to transfer the resistance to the mercury and drug sensitive recipients of E. coli K12 ML 1410-Nx and E. coli JE 17-Rif. Among 94 strains of Hg resistant K. pneumoniae, 86 R plasmids with Hg resistance could be demonstrated. It should be noted that transferable R plasmids with Hg resistance were demonstrated in 92% of the Hg-resistant isolates. Furthermore, R (Hg; SM, TC, CP, KM) plasmids were isolated most frequently, followed by R (Hg; SM, TC, CP), R (Hg; SM, KM), and R (Hg; SM, TC) plasmids.

Anti-Bacterial Agents↗
PubMed1977

Distribution of resistances to metals and antibiotics of staphylococcal strains in Japan.

Clinical isolates of 565 staphylococcal strains were studied for the resistance to four metals and eight antibiotics. Distribution patterns of their susceptibilities to Hg, As and Pb clearly revealed two peaks. But in the case of Cd resistance, there were three groups of Cd sensitivity. Frequencies of metal resistances were 36.6% to Hg, 95.6% to Cd, 48.8% to As and 91.9% to Pb. On the other hand, those of drug resistances were 87.3% to PC, 28.5% to SM, 33.5% to TC, 22.7% to CP, 23.9% to KM, 43.0% to EM, 38.8% to JM and 0% to GM. Most of tested cultures were metal-resistant (92.9%), moreover, these multiple-metal-resistant strains were also found to be multiple-drug-resistant. And about 10% of total isolates were metal-resistant and drug-senstitive strains, whereas the frequency of the metal-sensitive and drug-resistant strains was only 1.8%.

Anti-Bacterial Agents↗
PubMed1976

Unbalanced 13q/21q translocation: a revised study of the case previously reported as 21-monosomy.

Reexamination was made on a male infant previously reported as 21-monosomy. Extensive chromosome banding analyses in the patient and parents disclosed an unbalanced de novo translocation between chromosomes 13 and 21. The patient's karyotype was interpreted as 45,XY,--13,--21+der(13),t(13;21) (q2 or 3;q1 or 2)pat. The patient showed many clinical features characteristic of 13q--syndrome.

Aneuploidy↗
PubMed1975

Simple and effective method for selecting protein A-dificient mutants by consedimentation with sensitized sheep erythrocytes.

An effective slection method for protein A-deficient mutants was devised by means of cosedimentation with sensitized sheep erythrocytes and anti-protein A serum agar plates. Hitherto, two types of mutants have been successfully isolated by this method. One was deficient in both cell-bound protein A and soluble protein A. The other seems to release extracellularly soluble protein A but does not possess cell-bound protein A. Spontaneous mutants of the former type were contained in the culture of Staphylococcus aureus 248betaH in a concentration of approximately 10-minus-5, which is ten times as much as the latter. Repeated cosedimentation procedure greatly increased the efficiency of isolation of the mutants. As a result, a majority of the bacterial cells remaining in the final supernatant after three cosedimentation procedures were of the two mutant types. Details of the method as well as some preliminary results are described.

Animals↗
PubMed1975

Two serotypes of exfoliatin and their distribution in staphylococcal strains isolated from patients with scalded skin syndrome.

Two serotypes of exfoliatin, A and B, previously reported by Kondo et al. (1974) were examined for their presence in 43 strains of Staphylococcus aureus, most of which were isolated from patients with Ritter's disease and impetigo. The tested strains consisted of 24 strains of phage group II and 19 strains not of phage group II. Twenty-four strains were found to produce an exfoliatin of either type A or type B, but 16 strains produced both types and 3 strains produced neither. No relationship was found between the serotype of an exfoliatin and the phage type of the exfoliative strain, although the single producers of exfoliatin A were all found to belong to phage group II and those of exfoliatin B to the other phage group.

Bacteriophage Typing↗
PubMed1974

New type of exfoliatin obtained from staphylococcal strains, belonging to phage groups other than group II, isolated from patients with impetigo and Ritter's disease.

Four strains of Staphylococcus aureus of a phage type other than the second group, isolated from patients with impetigo and Ritter's disease, were found to produce an exotoxin similar to those reported by Melish et al. (1972), Kapral and Miller (1971), and Arbuthnott et al. (1973). This toxin could elicit a general exfoliation of the epidermis with the so-called Nikolsky sign when subcutaneously inoculated into neonatal mice within 4 days after birth. The new toxin was serologically different from exfoliatin produced by the phage group II staphylococci previously reported (Kondo et al., 1973) and showed an electrophoretic pattern corresponding to that of the B-type toxin of the latter in acrylamide disc electrophoresis. It had the same molecular weight as that of the latter, which was estimated to be about 24,000. It was thermolabile and lost its toxic activity by heating at 60 C for 30 min; in addition, most of the toxicity was lost within 1 month of storage even at -30 C. We propose to designate the old typical heat-stable exfoliatin as S. aureus exfoliatin A and the new heat-susceptible exfoliatin as S. aureus exfoliatin B.

Animals↗
PubMed1974

Mercury and cadmium resistances mediated by the penicillinase plasmid in Staphylococcus aureus.

Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat innocuous form. This process is independent of temperature and seems to be controlled by an inducible enzyme. The killing effect of Hg salts was not influenced by the coexistence of other di- or monovalent ions such as MgCl(2), CaCl(2), MnCl(2), and NaCl. No vaporization of Hg, which explains the resistance mechanism such as that proposed by Komura et al. for R factor-mediated Hg resistance in enterobacilli, was found in the case of Hg resistance in staphylococci. On the other hand, the resistance to Cd ion is mediated by some protective mechanism to retain the ion outside the cell. Pc-sensitive organisms not carrying the Pc-ase plasmid incorporate Cd ions into the cells, whereas the Pc-ase plasmid-carrying organisms do not. The incorporation of this ion is temperature dependent and does not take place at 4 C. When incubated with this ion at 4 C, Pc-sensitive organisms as well as Pc-resistant organisms are also able to show a resistance. The addition of CaCl(2) could eliminate the killing effect of CdCl(2) with a dose-effective response.

Cadmium↗
PubMed1973

Purification of exfoliatin produced by Staphylococcus aureus of bacteriophage group 2 and its physicochemical properties.

An effective method for the isolation and purification of exfoliatin which has been recently reported by Melish and others as the staphylococcal toxin responsible for the scalded skin syndrome and the physicochemical properties of the purified toxin were described. From an active crude toxin produced by one of the clinical isolates of phage group 2, four types of toxic proteins which were all capable of causing the typical Nikolsky sign in neonatal mice were obtained and designated A, B, C, and D toxins. They had a molecular weight of about 24,000 and showed the same serological features in neutralization and precipitation tests, but were different from each other in showing a different single band with their respective mobilities in polyacrylamide disk electrophoresis. They were precipitated between pH 4.0 and 4.5 and lost their exfoliative capabilities. The resulting precipitates, however, could be solubilized in acetate buffer containing 0.5% sodium dodecyl sulfate, restoring their toxicities to almost the same extent as before. They were all stable when heated at 60 C for 60 min and at 100 C for 20 min, but lost their toxicities when heated at 100 C for 40 min. Additionally, the present authors observed that some staphylococcal strains not belonging to the typical phage group 2, isolated from patients with the scalded skin syndrome, were also capable of producing a similar but serologically unrelated exfoliative toxin.

Animals↗