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Biomedical subjects

I Katayama

Publications and source records attributed to I Katayama.

At least 325 records · Page 18Linked to original sources
PubMed1983

Analysis of cells of the mononuclear phagocyte series in experimental mycobacterial granulomas by monoclonal antibodies.

Two distinct types of granulomas were produced in the draining lymph nodes by immunizing guinea pigs with Mycobacterium bovis BCG or Mycobacterium leprae, as reported earlier (Narayanan et al., J. Pathol. 134:253-265, 1981). In the BCG-induced granuloma there is successful containment, killing, and degradation of the organisms with the presence of epithelioid cells and fibrosis. M. leprae, on the other hand, induces a granuloma where there is an absence of organization of the cells, failure to completely degrade the organisms, absence of epithelioid cells, and minimal fibrosis. By using a macrophage-specific monoclonal antibody and an anti-Ia monoclonal antibody and applying the immunoperoxidase, immunofluorescence, and fluorescence-activated cell sorter analysis techniques, the epithelioid cells of the BCG granuloma were found to have macrophage-specific antigen, but not detectable amounts of Ia antigen. This suggests that these cells have a close relationship to other cells of the mononuclear phagocyte series with which they share a common antigen. The absence of Ia antigen, on the other hand, suggests that epithelioid cells may not be involved in antigen presentation or other accessory cell functions where the presence of Ia antigen is crucial. The macrophages in the M. leprae-induced granuloma expressed both macrophage-specific and Ia antigens.

Animals↗
PubMed1983

Suppression of contact sensitivity by IgG1 antihapten antibody. IgG1 antihapten antibody suppresses migration inhibitory factor production by lymph node cells.

Sera obtained from guinea pigs sensitized with 2,4-dinitrofluorobenzene (DNFB) showed suppressive activity on the migration inhibitory factor (MIF) production by the lymph node cells obtained from DNFB-contact-sensitized guinea pigs. A similar suppressive effect was achieved by the sera of 2,4,6-trinitrochlorobenzene- (TNCB-)sensitized guinea pigs. The suppressive factor in the sera was found to be IgG1 antihapten antibody. It suppressed MIF production dose-dependently during a period between 3 h before and 1 h after the antigen stimulation. Pretreatment of macrophages with IgG1 antihapten antibody abolished the MIF production of the lymph node cell. The antibody lost its suppressive activity after splitting off its Fc portion, and pretreatment of macrophages with Fc fragments of normal IgG1 interfered with the action of the IgG1 antibody. Therefore, the IgG1 antihapten antibody in the sera of contact-sensitized guinea pigs acted on macrophages through its Fc portion to inhibit MIF production by lymph node cells.

Animals↗
PubMed1983

Naphthol AS-D chloroacetate esterase reaction. Systematic modifications for optimization to smears and sections.

Currently used methods for naphthol AS-D chloroacetate (NASDCA) esterase reactions can be divided into 3 groups according to the coupler used, i.e., fast garnet GBC, pararosanilin, and new fuchsin. These 3 groups were systematically modified with regard to modes of fixation and incubation in order to develop optimal methods for their application on sections of formalin-fixed and paraffin-embedded tissue as well as blood smears. After obtaining the optimal modifications for each of the 3 coupler methods, comparison was made between the 3 optimal methods for blood smears and the 3 for tissue sections. For blood smears, the methods using either fast garnet GBC or new fuchsin as coupler were found to be equally excellent, while the method using pararosanilin as coupler was not as superior. For tissue sections, the methods using either fast garnet GBC or pararosanilin were found to be equally excellent, while the method using new fuchsin was not as superior. Thus, only the method using fast garnet GBC as coupler was found excellent for both purposes. It was also found that bone marrow biopsies routinely processed through formalin-fixation would yield excellent NASDCA esterase reaction provided Plank and Rychlo's method instead of EDTA was employed for decalcification.

Biopsy↗
PubMed1982

Primary nodular lymphoma of the lung. Immunoperoxidase and ultrastructural studies.

In two patients, primary nodular lymphomas of the lung which presented incidentally were diagnosed following routine chest radiography, exploratory thoracotomy, and the application of histologic criteria. The diagnoses were confirmed using the peroxidase-antiperoxidase (PAP) technique and transmission electron microscopy. Whereas most investigators had demonstrated intracytoplasmic immunoglobulins in only the large cells of nodular lymphomas using the standard PAP method, we were able to prove the presence of monoclonal immunoglobulin within the cytoplasm of both the large cells and small cells using a modification of the PAP technique as described by DeLellis. This modification uses very dilute antisera and a prolonged incubation time and is more highly sensitive and specific. Monoclonal immunoglobulin was also shown to be within the cytoplasm of mature plasma cells observed within the interfollicular areas. This monoclonal immunoglobulin was the same as that seen within the cytoplasm of the small and large cells of each respective lymphoma. Electron microscopy permitted the identification of three cell types within each patient's tumor: small cells, large cells, and dendritic reticulum cells((DRCs). The finding of the DRCs, presently considered a residual marker of the germinal center, is supporting evidence that both lymphomas studied were nodular lymphomas of B cell derivation.

Female↗
PubMed1982

Non-IgG1 nature of cutaneous basophil hypersensitivity factor in contact sensitivity.

Cutaneous basophil hypersensitivity (CBH)-inducing factor was demonstrated in immune sera obtained from dinitrofluorobenzene (DNFB)-sensitized animals 2 weeks after sensitization (DNP-GPS-2W). It showed hapten specificity and worked dose dependently. It was fractionated into a non-gamma-globulin fraction by Sephadex G-150 gel filtration following ammonium sulfate desalting and CM-cellulose chromatography. The factor was eluted into a fraction of a little smaller molecular weight than bovine serum albumin on Sephadex G-150 gel filtration. It passed through an antiguinea pig IgG1 column and was absorbed to a DNP-BGG column. On SDS-PAGE it failed to show any staining band because of low protein concentration. From these results CBH factor appearing in circulation in contact-sensitized animals was thought to be a somewhat different molecule from that of Askenase's factor, i.e. IgG1 antibody.

Animals↗
PubMed1982

Wegener's granulomatosis: successful treatment with high-dose cyclophosphamide.

A 30-year-old male taxi driver developed extensive purpuric necrotic lesions all over his body. He had a consolidated shadow in the right upper lung field, proteinuria with microscopic hematuria, and granulomatous change in the nasal mucosa. The standard regimen of prednisolone alone or in combination with cyclophosphamide showed only a limited effect on the lesion. However, weekly intravenous injections of a high dose of cyclophosphamide (500 mg/week) in combination with 60 mg/day of prednisolone exhibited dramatical suppression of the disease activity.

Adult↗
PubMed1982

In vivo macrophage suppression of delayed hypersensitivity in the guinea-pig.

The nature of the suppressive activity in the peritoneal exudate cells (PEC) of guinea-pigs immunized with dinitrophenyl bovine gamma globulin (DNP50-BGG) was investigated. A method was developed to isolate from the peritoneal exudate large numbers of macrophages. Using density gradient centrifugation on Percoll it was possible to obtain a population of cells which contained over 90% macrophages. This macrophage preparation was found to respond to lymphokine but to be incapable of passively transferring delayed hypersensitivity reactions. When these immune macrophages were transferred into antigen immunized animals, which had been pretreated with cyclophosphamide (CY), the skin reactions were suppressed to the same extent as when the total PEC was transferred. PEC from guinea-pigs immunized with ovalbumin in Freund's incomplete adjuvant did not suppress the skin reactions in CY-pretreated DNP50BGG immunized animals. However, in contrast, macrophages from these animals did suppress the skin reactions in the recipient guinea-pigs indicating that the macrophage suppression was not antigen specific.

Animals↗