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Biomedical subjects

I Katayama

Publications and source records attributed to I Katayama.

At least 307 records · Page 17Linked to original sources
PubMed1984

Non-IgG1 nature of cutaneous basophil hypersensitivity factor in contact sensitivity. II. Demonstration of antigen-dependent basophil chemotactic activity of cutaneous basophil hypersensitivity factor.

It was recently demonstrated that there was a specific activity to induce basophil-rich skin reaction in the sera of contact-sensitized guinea pigs (CBH factor, CBH-F). In the in vitro chemotactic assay system, CBH-F was shown to have weak basophil chemotactic activity but enhanced its activity in the presence of corresponding antigen(s). Furthermore, basophil chemotaxis in reaction to the antigen(s) was observed when the cells were preincubated with CBH-F. It worked mainly for Percoll-separated bone marrow basophils but not for oil-induced peritoneal macrophages. Immunological analysis revealed that CBH-F was a protein with a small molecular weight (MW 4-6 X 10(4] with an antigen binding site and isoelectric point of between 4.5 and 5.0. It did not show any characteristics of IgG1 or IgG2 on immunoadsorbent column and immunoelectrophoresis. Enzyme treatment with insoluble trypsin eliminated the chemotactic activity but this was not the case with neuraminidase treatment.

Animals↗
PubMed1984

Eosinophilic spongiosis in bullous pemphigoid.

Eosinophilic spongiosis is accepted as one of the histopathological features of pemphigus. We found eosinophilic spongiosis in six (24%) of 25 patients in whom erythematous lesions, which were in the vicinity of blisters of bullous pemphigoid, were found. This finding was associated with eosinophilic infiltration of the upper dermis and peripheral eosinophilia.

Aged↗
PubMed1983

PAP immunoperoxidase method demonstrating endogenous estrogen in breast carcinomas.

A PAP immunoperoxidase method for demonstrating estrogen receptors in breast carcinomas is introduced. Whereas other methods pretreat the specimen with exogenous estrogen in high (unphysiologic) doses, this method demonstrates endogenous estrogen without such pretreatment. Experiments showed endogenous estrogen remaining in the formalin-fixed, paraffin-embedded breast tumors in sufficient amounts to be detected by the PAP method. The method can discriminate hormone responsive cells from hormone secreting cells because most responsive cells demonstrate both the cytoplasmic and nuclear stain and most secreting cells only the cytoplasmic stain. The method was applied to 277 human breast carcinomas, of which 197 (71.1%) were positive and 80 (28.9%) negative. These figures were comparable to those obtained by the biochemical methods. This PAP method may serve as an alternative to the biochemical assays of estrogen receptors.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1983

Analysis of transformation with Epstein-Barr virus and phenotypic characteristics of lymphoblastoid cell lines established from patients with hairy cell leukemia.

In order to assess the role of Epstein-Barr virus (EBV) in patients with hairy cell leukemia (HCL), we have sought to characterize 1) the ability of EBV to infect and transform hairy leukemic cells in vitro and 2) the phenotypes of cell lines putatively derived from those leukemic cells. Analysis of EBV-induced transformation and the kinetics of Epstein-Barr nuclear antigen (EBNA) induction in leukemic preparations indicated that most leukemic cells were not susceptible to EBV infection but that at least a small subpopulation of leukemic cells could be infected with EBV. Lymphoblastoid cells lines were established after exposure of peripheral blood or splenic cells from HCL patients to B95-8 or QIMR-WIL EBV. Splenic leukemic cell preparations were more sensitive targets for EBV transformation than were peripheral blood cell samples. The newly established cell lines, but not long-established B lines such as Raji, demonstrated high levels of synthesis of p35, (a protein complex expressed abundantly by cells of a subset of HCL patients) and high levels of tartrate-resistant acid phosphatase (an enzyme relatively diagnostic for HCL). Lymphoblastoid lines from one patient with HCL expressed lambda light chains and no kappa chains as did the patient's leukemic cells. Virus expression in these lines showed that HCL-derived lines had spontaneous early antigen (EA) and viral capsid antigen (VCA) expression. Transforming EBV could be rescued from HCL-derived cell lines but not from cord blood-derived lines.

Acid Phosphatase↗
PubMed1983

Macrophage specific antigen is expressed by resting microglia in the CNS but not by Langerhans cells in the skin.

Controversy exists as to whether Langerhans cells in the epidermis and resting microglia in the brain should be included among cells of the mononuclear phagocyte series (MPS). A monoclonal anti-guinea-pig macrophage antibody has been prepared that is specific for a macrophage membrane antigen and does not react with Fc receptors or Ia antigens. This antibody fails to react with Langerhans cells despite reacting with peritoneal exudate macrophages, alveolar macrophages, Kupffer cells and macrophages in infectious granulomas. It does, however, react with resting microglia in the brain. This could suggest that Langerhans cells, despite a similar bone marrow origin, are not typical cells of the MPS, whereas resting microglia share features with this cell system.

Animals↗