Primary omental torsion in a child: mimic acute appendicitis.
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Appendicitis↗
Biomedical subjects
H Wang
Publications and source records attributed to H Wang.
[Study on the enantiomeric separation of adrenalines by capillary zone electrophoresis].
Capillary zone electrophoresis (CZE) has been used for the enantiomeric separation of the racemic isoprenaline, noradrenaline and adrenaline with bare fused silica capillary and employing beta-cyclodextrin(beta-CD) and beta-cyclodextrin derivatives as the chiral selectors. Both the complexion and enantiomeric resolution were influenced by the temperature, beta-CD type, CD concentration and pH of background electrolyte (BGE). The effects of the BGE types and concentrations on the enantiomeric separation were also investigated. The results showed that 2,6-di-O-carboxymethyl-beta-cyclodextrin (CM-beta-CD) has stronger recognition than that of beta-CD for the chiral separation of the basic drugs under study. The CD concentrations and pH of BGE have strong influence on the efficiency of chiral separation. The adsorption of the basic compound on the surface of bare fused silica capillary is a disadvantage to the chiral separation while the use of beta-CD as the chiral selector and the addition of an amphiphile, such as tetraethylammonium bromide (TEA+) or tetrabutylammonium bromide (TBA+) to the BGE could improve the resolution of the enantiomers due to the reduction of the adsorption of the basic compound on the silica surface by the strong electrostatic force between the positively charged amphiphile and the silica surface. It was also found that as CM-beta-CD was used as the chiral selector there was no influence on the chiral separation of the basic compound whether the amphiphile was added to BGE or not. Lower temperature was favorable to improve the efficiency of the chiral separation. CM-beta-CD gave a baseline enantiomeric separation for isoprenaline, while beta-CD, under the same experimental conditions, gave an incomplete chiral separation. Both CM-beta-CD and beta-CD could not give enantiomeric resolution for noradrenaline and adrenaline.
Electrophoresis, Capillary↗
[Effect of orphanin FQ on acupuncture analgesia and noxious stimulation in the periaqueductal gray].
Orphanin FQ (OFQ) is a newly-discovered neuropeptide which shares great similar sequence with endogenous opioid peptide but with different functions. The present study was to investigate the role of OFQ microinjected in the periaqueductal gray (PAG) on pain threshold and acupuncture analgesia (AA) using tail flick tests. The results show that the hyperalgesia of OFQ and its antagonization on AA in PAG are dose-related. OFQ in PAG decreases the enhancing effect on AA induced by ohmefentanyl, a selective mu-receptor agonist. These observations suggest that OFQ in PAG can increase the rat pain sensitivity and antagonize AA.
Acupuncture Analgesia↗
[Effects of exogenous wild type p53 on malignant growth of human lung cancer cell line].
OBJECTIVE: To study the effects of exogenous wild type p53 suppressor gene on malignant growths of human lung cancer cell line with mutant type p53 gene. METHOD: Four human lung cancer cell line were screened for mutations in the exon 5 through exon 8 of the p53 tumor suppressor gene with immunohistochemistry, polymerase chain reaction (PCR)/single strand conformation polymorphism (SSCP) and DNA sequence analysis. The recombinent plasmid PZIPneoSV-p53 was constructed, which express wild type p53 gene. A transfected cell line, 801-D-p53, was obtained after transferred the plasmid into 801-D cell line by gene gun mediated and selected by G418. The exogenous p53 in the transfected cell line 801-D-p53 were inspected with PCR, and the alteration of growths of the transfected cell line in vitro and in vivo was observed. RESULT: The point mutation were CGG to CTT transversion at codon 248 in exon 7 was found in human lung cancer cell line 801-D by PCR-SSCP and DNA sequence analysis and nuclear accumulation of the p53 protein was observed. The neo gene and exogenous wild type p53 gene were detected in the transfected cell line 801-D-p53. The cell growth experiment in vitro showed that the parent cell line 801-D growth was very fast, from 1 x 10(5)/ml to 2.5 x 10(5)/ml within 6 days, the transfected PZIP-neo-SV cell line (plasmid without p53) growth as fast as 801-D, but the transfected cell line 801-D-p53 growth was inhibited seriously. The clonogenic formation rate of the parent cell line 801-D, transfected cell line 801-D-PZIP and the transfected cell line 801-D-p53 were 11.2%, 11.4% and 0.46% respectively. The clononogenic formation inhibition rate of the transfected 801-D-p53 was 96% comparing with the parent 801-D cell line. In the experiment of xenogenic tumor transplantation, each cell line was injected subcutaneously into four mice and the growth of xenogenic tumor transplant in nude mice was observed. xenograft growth in all 4 mice in both 801-D and 801-D-PZIP groups, but only 1 xenograft growth among 4 mice of 801-D-p53 group during 2 months. The average volume of xenogenic tumor transplant of 801-D and 801-D-PZIP groups were 6.500 cm3 and 2.231 cm3 respectively and the only xenograft volume of 801-D-p53 group was 0.940 cm3. The tumorigenicity of 801-D-p53 in nude mice was significantly suppressed. CONCLUSION: Exogenous wild type p53 gene may stably exist in the human lung cancer cell line with mutant type p53 after plasmid transfection and suppressed the malignant growth of the transfected cell line 801-D-p53 in vitro and in vivo. These results indicate that the recombinent plasmid expressing wile type p53 may be useful for gene therapy of human lung cancer.
Animals↗
[The effect of herbal medicine including astragalus membranaceus (fisch) bge, codonpsis pilosula and glycyrrhiza uralensis fisch on airway responsiveness].
OBJECTIVE: To study the effect of astragalus membranceus(fisch) bge, codonpsis pilosula and glycyrrhiza uralensis fisch on airway responsiveness. METHOD: FVC, FEV1 and PEF of twenty-eight asthmatic patients were measured before and six weeks after treated with Astragalus membranceus(fisch) bge, codonpsis pilosual and glycyrrhiza uralensis fisch; methacholine challenge test with 0.033 mumol and 1.98 mumol. RESULT: After 28 asthmatic patients received the treatment of those herbs, the results showed that FVC value increased obviously (t = 2.217, P < 0.05) and the value of FEV1 and PEF was all higher compared with before treatment of the herbs (P < 0.01). CONCLUSION: The herbal medicine including astragalus memberanaceus (fisch) bge, codonpsis pilosual and glycyrrhiza uralensis fisch can decrease the airway responsiveness of the patients with asthma.
Adolescent↗
[Comparison of polymerase chain reaction and culture method for assaying ureaplysma urealyticum in urogenital specimens].
OBJECTIVE: A comparison study of polymerase chain reaction (PCR) and culture method were used to detect urogenital ureaplasma urealyticum. METHODS: Urogenital specimens from 116 patients were assayed in parallel by the two methods. RESULTS: 46 cases were positive by PCR, 42 cases positive by culture, and UuDNA were dedected in 42 cases of the 45 positive by culture. Compared with culture, the sensitivity and specificity of PCR were 93.3% and 94.4%, respectively. The positive rate of both methods were comparable (P > 0.05). CONCLUSIONS: The PCR technique to detect Uu is highly sensitive and specific. As for culture method, it takes the advantage of being relative simple and takes much less time to give results.
Adolescent↗
[Reconstruction of transcriptional regulatory elements of human alpha-fetoprotein gene].
OBJECTIVE: To construct vectors that express therapeutic genes specifically in hepatocellular carcinoma, the TREs (Tissue-specific transcriptional regulatory elements) of natural human alpha-fetoprotein (AFP) was reconstructed. METHODS: Using subcloning techniques, the -870 bp(-)-3.06 kb fragment of the natural human AFP TREs was deleted to construct an artificial AFP(e/p) TREs which contains the "core" promoter and the -3.06 kb(-)-5.1 kb fragment of natural human AFP TREs that contains enhancer activity. RESULTS: By using chloramphenicol acetyltransferase (CAT) assay, the artificial AFP(e/p) TREs showed an activity 1.8 fold as high as that of the natural AFP TREs in an AFP positive human hepatocarcinoma cell line HepG2, and it also remained to be tissue-specific since there was no activity was detected in an AFP negative human larynxcarcinoma cell line Hep2. CONCLUSIONS: These results indicate that the artificial AFP(e/p) TREs we have constructed has higher activity than the natural AFP TREs and it also remains its hepatocarcinoma specificity. The reconstructed AFP(e/p) TREs is suitable for constructing of gene therapy vector with hepatocarcinoma expression specificity.
Carcinoma, Hepatocellular↗
[Clinical observation on effect of comprehensive immunotherapy in treating hepatic carcinoma after embolism chemotherapy].
OBJECTIVE: To probe the relationship between survival period and immune function of hepatic carcinoma patients after embolism chemotherapy. METHODS: One hundred and eight patients of hepatocarcinoma were randomly divided into three groups. Group A was given simple embolism chemotherapy (ECT), immunotherapy and Chinese herbal medicine were given to Group B and C respectively after ECT. RESULTS: The short-term effective rate of Group A, B and C was 61.1%, 83.3% and 80.6% respectively, three-year survival rate 28.6%, 39.1% and 47.3%, median length of survival 11, 17 and 15 months respectively. The difference in terms of these indices were significant. Observation on immune function after treatment showed that patients received ECT alone had a lower immunity than those supplemented with immunotherapy or Chinese herbal medicine. CONCLUSIONS: Period of survival of hepatocarcinoma patients is related with their immune function. Patients with better immunity could enjoy a relatively extended period of survival.
Adolescent↗
[Clinical study on the forced oscillatory pulmonary function test].
OBJECTIVE: To discuss the change of airway resistance and phase angle (phi) in normal subjects, patients with COPD, asthma and pulmonary fibrosis when the new technique of the forced oscillatory pulmonary function test was applied to measure them as well as the change of pulmonary function of patients with asthma after using bronchodilator. METHOD: Use Custo-vitm forced oscillation device manufactured in Germany to determine airway resistance and phase angle and to compare their statistical differences between normal subjects and patients with pulmonary disease, and then comparing the improvement rates of their FEV1, F-V curve and airway resistance in patients with asthma after inhaling bricasol. RESULT: The measurements of group COPD and asthma were significantly higher than those of normal group (P < 0.05), and there were obvious frequency dependence in these two groups. The results of phase angle in each group of patients were significantly lower than those of normal group (P < 0.05), and the group of patients with pulmonary fibrosis only demonstrates a decrease in phase angle, and the improvement rate of airway resistance in patients with asthma after inhaling bricasol was significantly higher than FEV1 and F-V curve (P < 0.05). CONCLUSION: Forced oscillation is a convenient, quick, and noninvasive new method when applied to measure airway resistance and phase angle. It is especially suitable for patients with severe COPD and better than conventional pulmonary function test in observing therapeutic effect in asthma.
Adult↗
[Chemical constituents of radix Angelicae Sinensis].
Six compounds were isolated from Radix Angelicae Sinensis and identified as (E)-ligustilide, (Z)-ligustilide, (Z)-n-butylidene phthalide, palmitic acid, beta-sitosterol and ferulic acid.
4-Butyrolactone↗
[Protective effects of folium Ginkgo extract on experimental cerebral ischemia of mice].
Folium Ginkgo extract(EGb761) has been proved able to significantly prolong the survival time of mice suffering from cerebral ischemia induced by occluding bilateral common carotids, and reduce the inhibition of cerebral GSH-Px and Na(+)-K(+)-ATPase activities during reperfusion processes of these mice. Pretreatment with EGb761 also helps alleviate the subcellular damages in hippocampus of cerebral ischemic mice.
Animals↗
[Chemical constituents of Rheum wittrochii Lundstr(II)].
OBJECTIVE: To study the chemical constituents in the root of Rheum wittrochii. METHOD: Chromatography and spectroscopic analysis to isolate and elucidate the chemical constituents in the plant. RESULT: Six compounds, namely 3, 5-dihydroxy-4'-methoxystilbene; 3, 5, 4'-tridroxystilbene; piceatannol; desoxyrhaponticin; piceatannol-4'-O-beta-D-glucopyranoside; 2, 5-dimethyl-7-hydroxychromone, have been isolated from ethyl ether and ethyl acetate fractions. CONCLUSION: These compounds were isolated from the title plant for the first time.
Drugs, Chinese Herbal↗
[Chemical constituents of Rheum wittrochii Lundstr(I)].
OBJECTIVE: To study the chemical constituents in the root of Rheum wittrochii. METHOD: Chromatography and spectroscopic analysis were used to isolate and elucidate the chemical constituents in the plant. RESULT: Six compounds, namely chrysophanol, physcion, emodin, chrysophanol-8-O-beta-D-glucopyranoside, physcion-8-O-beta-D-glucopyranoside and beta-sitosterol, have been isolated from petroleum ether and ethyl acetate fractions. CONCLUSION: All the above-cited compounds were isolated from the title plant for the first time, and anthraquinone compounds are some of the major constituents of R. wittrochii.
Anthraquinones↗
[The influence of implantal autograft bone in total cleft palate on maxillary growth an experiment study in dogs].
OBJECTIVE: To test the hypothesis that implant autograft bone in total cleft palate at primary cleft palate repair which may resists the disturbance of maxillary growth by cleft and denud bony wound. METHODS: 40 dogs were randomly divided into four groups: three control groups and one experimental group. The first control group was unoperated, the second group had a surgically created cleft plate, the third group also had a surgically created cleft palate. Using a two-flap procedure immidiately, the experiment group had a surgically created cleft palate, but the palate was recontracted by implanting a piece of autograft rib bone with two-flap repair. All animals were sacrificed on the 34th week after operation (43 weeks postnatal). Thirty-four metricranial variables were measured directy from the cleaned skulls. RESULTS: The method is useful for maxillary growth and development by bone implant in total cleft palate, but it can cause maxillary deformity. CONCLUSION: Clinical doctors should be cautious in using the method of primary-delay bone grafting in reparing total cleft of cleft palate.
Animals↗
[Occlusion and temporomandibular joint dysfunction syndrome].
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Dental Occlusion↗
[An experimental study on the bone repairing ability of recombinant human bone morphogenetic protein-2-coral composited artificial bone].
OBJECTIVE: To improve the bone repairing ability of coral. METHODS: Recombinant human bone morphogenetic protein-2 (rhBMP-2)-coral composited artificial bone was used. The composite was implanted into rabbit calvarial critical-size defects, and coral alone was implanted as control. The rabbits were sacrificed at 2, 6, 12 weeks respectively after operation. The bone repairing ability was assessed by histology and image analysis system. RESULTS: The composite repairing the bone defect both in osteoconductive and osteoinductive manners was seen, repairing ability and effect were better than those of coral. CONCLUSION: The composite is ideal as a bone graft substitute.
Animals↗
[Effects of overextended mouth opening on substance P-like immunologic reaction of TMJ soft tissues].
OBJECTIVE: To probe deeply into the traumatic characteristics and the neurobiologic mechanism of overextended mouth opening of the temporomandibular joint (TMJ). METHODS: Changes of the substance P-like immunologic reaction (SPIR) of the TMJ soft tissues were examined with ABC immunohistochemical staining along with the histopathological study of the overextended opening. RESULTS: An obvious increase of SPIR brought about by overextended opening was mainly in the anterior extending tissue of the disc, the bilaminar zone and the subsynovial layer of the articular capsule, and the extent of the reaction became greater with the increased duration of over-opening. The SPIR decreased obviously two weeks after removing the nocuous stimulation. The changes of SPIR almost coincided with the histopathological changes in sites and extents, and in the effects on them of the relevant treatment, which indicate that there are some internal relations between the two kinds of changes. CONCLUSION: To recognize the characteristics of the changes and grasp the internal relations will help us to find new approaches to the prevention, diagnosis and treatment for the trauma and pain of the TMJ.
Animals↗
[Long-term follow-up study of postoperative revascularization in childhood moyamoya disease: results of 226 cases].
OBJECTIVE: To study the long-term results of 226 patients with moyamoya disease ased below 15 years and to report the modified method of extra-intracranial arterial anastomosis and encephalo-myo-arterio-syn-anastomosis, etc. METHOD: The mean follow-up period was 15.5 years (range 8 - 19 years). The results were obtained from the pre- and postoperative cerebral angiographic examination, MRI, CT, cerebrovascular Doppler ultrasonography, Glasgow Outcome Scale (GOS), intelligence quotient (IQ) as classified by Taft and neurological symptoms. RESULT: Of the 226 patients with childhood moyamoya disease, 64 (28%) recovered in completely, 69 (31%) in improved markedly, and 46 (20%) improved slightly. 43 patients (19%) had no changes and 4 (2%) died. The effective rate of the operation was 79%. CONCLUSION: The modified direct anastomosis and encephalo-myo-arterio-synagiosis played a role in this improvement by increasing CBF after the operation. A significant correlation is found between the postoperative effect and the stages of preoperative angiograms.
Adolescent↗