A definite case of diffuse panbronchiolitis diagnosed by open lung biopsy.
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Adult↗
Biomedical subjects
H Wang
Publications and source records attributed to H Wang.
24-hour gastroesophageal double pH monitoring acid and alkaline gastroesophageal and duodenogastric refluxes in pediatric patients.
OBJECTIVE: To study the pathophysiologic significance of gastroesophageal and duodenogastric-esophageal refluxes in pediatric patients. METHODS: Gastroesophageal double pH monitoring was performed on 68 children with gastroesophageal reflux (GER) diseases and 39 normal children. The pH shifts in the intra-gastric and esophageal lumina were recorded for a total of 24 hour period (P1) and for the period of gastric empty (P2) in supine and upright body postures. RESULTS: The following reflux types were identified: acid GER in 40 cases (58.8%), alkaline GER (AGER) in 8 cases (11.8%), mixed GER (MGER) in 14 cases (20.6%) and silent GER (SGER, i.e., reflux with normal pH values in P1) in 6 cases (8.8%) as well as duodenogastric reflux (DGR). The results showed a number of transient GER and DGR (19 of 39 children) in the control group, recorded mainly in the upright position at meal times and 2 hours postprandially. All pH variables for acid reflux were higher in the acid GER and MGER groups than those in the control group (P < 0.01). The alkaline episode was significantly noted in cases with AGER and MGER in the supine position during P2. There was no significant difference in terms of incidence of esophagitis between GER groups. The gastroesophageal double pH monitoring produces the higher positive diagnostic rate of 91.2% than single esophageal pH testing (79.4%). CONCLUSIONS: Transitory GER and DGR at meal times and 2 hours later might be a physiologic phenomenon. Acid and alkaline reflux occurring in the supine position during P2 should be considered of pathologic significance. This combined gastroesophageal pH monitoring is used not only to improve the diagnostic rate but also to guide clinicians to choose efficient anti-reflux therapy based on the type of reflux as well as to provide refined information for the further study of the pathophysiology of duodenogastroesophageal reflux.
Adolescent↗
Linkage analysis of the genetic markers of the candidate genes with non-insulin dependent diabetes mellitus in Chinese families.
OBJECTIVE: To explore the relationship between the genetic markers of the candidate genes (insulin receptor, glucose transporter 2 and glucokinase) and non-insulin dependent diabetes mellitus (NIDDM) in Chinese pedigrees. METHODS: Genomic DNA of 87 members in 12 Chinese NIDDM pedigrees was amplified using PCR technique. Denaturing polyacrylamide gel electrophoresis was applied to detect short tandem repeat polymorphisms. The linkage program was used under the models of autosomal dominant and recessive. The individuals with impaired glucose tolerance (IGT) of age < or = 45 and age > 45 were counted as affected and unknown status respectively. RESULTS: Linkage of insulin receptor gene and NIDDM was strongly and significantly rejected with total LOD score of-infinity (theta = 0.000). Linkage analysis of glucose transporter 2 gave the result with total LOD score of 2.646 (theta = 0.000) in 4 pedigrees. Linkage of glucokinase and NIDDM was of significance with total LOD score reaching 3.150 in the integration of 6 pedigrees. CONCLUSIONS: Mutations in or near the insulin receptor gene are unlikely to be the major cause of inherited predisposition to NIDDM in Chinese pedigrees that are related in this study, not excluding the principal role in the other Chinese or populations, while the mutations/variations near or at the glucose transporter 2 gene locus might be something of the cause, which needs more data to be ascertained, of familial NIDDM in Chinese pedigrees. However, the variations in or near the glucokinase gene might play a dominant role in the development of NIDDM in some Chinese and represent a subgroup of common NIDDM.
Adult↗
Construction of human insulin gene expression recombinant and its effect on blood glucose of diabetic rats.
OBJECTIVE: To study the effect of human insulin gene modified fibroblasts on blood glucose in diabetic rats. METHODS: An expression vector containing human insulin gene was constructed by recombinant DNA technique and introduced into fibroblast Ltk- cells by lipofectin-mediated DNA transfection. Following G418 screening, the survived cells were selected and enriched. Finally a cell line, PRI-12 was generated for the highest insulin production. Then, these cells were injected into streptozocin (STZ)-induced diabetic rats. RESULTS: Insulin DNA transfected Ltk- cells were able to express insulin at a high level in a long-term culture. Furthermore, these Ltk- transfectants could decrease blood glucose significantly (P < 0.01) and obviously increase the body weight (P < 0.01) when they were injected into STZ-induced diabetic rats. CONCLUSIONS: These results suggest that the cell lines transfected insulin gene could secrete insulin and execute effect on diabetic rats. The study provides support for the view that somatic cell gene therapy offers a potential approach to delivery insulin into diabetes mellitus.
Animals↗
[A modification to traditional combined approach tympanoplasty].
A modified method of traditional combined approach tympanoplasty (CAT) was performed on for 86 cases (89 ears). The patients were followed-up on for 18-50 months. No recurrent cholesteatoma or cochlear hearing impairments were found, and all the tympanic membranes had healed. The results of pure tone hearing tests showed that 66 ears (74%) had partial hearing (higher than 30 dB HL level), 56 ears (63%) had a small gap between BC and AC (< 10 dB HL), 10 ears (11%) had no hearing improvement. The total effective rate (the pure tone hearing improved more than 10 dB HL after operation) was 89% (79 ears). The clinical application of this kind of modified operation method had shown its important significance in thoroughly removing the diseased tissue, in preventing recurrent or residual cholesteatoma, in substantial hearing reconstruction and in improving the function of tympanum. Also the indication of combined approach tympanoplasty has been extended.
Adolescent↗
[Clinical and genetic analysis of a severe vWD pedigree].
OBJECTIVE: To investigate clinical features and analyse defect gene transmission in a severe vWD pedigree. METHODS: Phenotypes of a severe hemorrhagic pedigree including 26 family members of four generations were diagnosed with vWF multimer banding and ELISA. Defective gene transmission among the family members was investigated with a series of RFLP and VNTR loci within vWF gene. RESULTS: For the proband, plasma vWF:Ag was less than 2%, and multimer banding was negative. Gene analysis showed that she carried 2 different defective vWF haplotypes from her parents. The family members carrying one of the defective haplotypes showed normal or slight decrease of vWF:Ag and normal multimer banding pattern. CONCLUSION: 1. The proband could be regarded as a putative compound heterozygote of type 3 vWD. The defect vWF gene carried by heterozygote family members could be compensated by a normal allele. 2. DNA analysis based on polymorphism markers within vWF gene has practical values in genetic consulting of severe vWD pedigrees.
Adolescent↗
[A novel missense mutation in the B domain of factor VIII causes severe hemophilia A].
OBJECTIVE: To identify the genetic defect of a patient with severe hemophilia A (SH9). METHODS: PCR, denaturing gradient gel electrophoresis (DGGE) and DNA sequencing were used to screen mutations in the factor VIII gene. Intron 22 inversion was excluded previously by Southern blotting with F8A probe. PCR primers were designed to cover all the coding regions and flanking intron sequences. Amplified products were analysed with DGGE, and bands of abnormal mobility were directly sequenced. RESULTS: PCR fragment 14-2 showed slower mobility than normal. A single nucleotide substitution C2535A causing a missense mutation in the B domain, 826Asp(GAC)-->Glu(GAA) was identified by DNA sequencing. CONCLUSION: This nucleotide substitution may be the molecular etiology of SH9. The possible molecular mechanisms underlying this candidate mutation is yet to be clarified.
Factor VIII↗
[A preliminary study on the in vitro eukaryotic expression of human factor VIII cDNA].
OBJECTIVE: To evaluate the expression efficiency of a cDNA sequence of human clotting factor VIII (4.7 kb, B domain-deleted) in in vitro systems. METHODS: After insertion of the cDNA into several mammalian expression vectors, such as retroviral vector pMSCV, EB virus-based vector pGRE5.2/EBV and eukaryotic expression plasmid pCI, the expression of these constructs were tested in a variety of cells. RESULTS: All the three kinds of constructs-pCI-VIII, pGRE5.2/EBV-VIII and pMSCV-VIII were able to direct FVIII synthesis in NIH3T3, Hela and Bosc23 cells, respectively, while the pMSCV-VIII and pGRE5.2/EBVVIII produced relatively high levels of FVIII activity (up to 0.7 units/ml and 2.0 units/ml from 24 h to 48 h, respectively, after transfection with lipofectamine). The three forms of pMSCV-VIII vector worked in a similar efficacy in Bosc 23 cells, but this function was not detected in NIH3T3, psi-Crip and GP + E86 as well as 32DC13 cells in a transient transfection assay. Moreover, the NIH3T3 and 32DC13 cells infected with culture supernatant from pMSCV-VIII transfected-Bosc23 cells (as packaging cells) unexpectedly did not produce detectable FVIII activity. CONCLUSION: Apart from the design and construction of vectors, target cell selection may play a crucial role in the efficient expression of the FVIII cDNA.
3T3 Cells↗
[Tissue factor expression during all-trans retinoic acid or arsenic trioxide treatment in acute promyelocytic leukemia].
OBJECTIVE: In order to study the effect of all-trans retinoic acid (ATRA) or arsenic trioxide (As2O3) treatment on the expression of tissue factor (TF) in acute promyelocytic leukemia(APL). METHODS: The plasma level of soluble fibrin monomer complex(SFMC) and D-dimer(D-D), and the TF level of cell lysate were measured by ELISA, the transcription of TF mRNA was assessed by RT-PCR. RESULTS: The plasma level of SFMC and D-D, the procoagulant activity(PCA) of bone marrow blasts, the TF level of cell lysate and the transcription of TF mRNA all remarkably elevated at diagnosis, while reduced after ATRA or As2O3 therapy. CONCLUSION: Both ATRA and As2O3 downregulated the expression of TF mRNA, decreased the PCA and TF levels in APL cells, inhibited coagulation activation and secondary hyperfibrinolysis, thus greatly relieved the bleeding symptom in the early stage of treatment.
Adolescent↗
[Linkage analysis of vWD family by multi-PCR assay of microsatellite DNA in vWF gene].
OBJECTIVE: To set up a convenient method for analysis of the amplified fragment length polymorphism (amp-FLP) of two microsatellite DNA in vWF gene. METHODS: Multi-PCR followed by denatured PAGE and silver stain were used to analyze the amp-FLP of two locus (A and B) on one gel. DNA samples from 112 normal people and two vWD families in the Shanghai area were assayed. RESULTS: 1. seven and 5 types of amp-FLP were identified on loci A and B, respectively. The heterozygote rates were 75% and 74%, respectively. 2. Haplotypes A2/B3 and A4/B3 were found to link with defective vWF genes in the two vWD families, respectively. CONCLUSION: 1. Multi-PCR is a fast and practical method to carry out family analysis of inherited diseases. 2. nt1980-1990 and nt2215-2380 of vWF gene are two ideal genetic labels in linkage study and hereditary consultation of vWD family.
Child↗
[RBC-CR1 genotype polymorphism and RBC immune function in childhood ITP].
OBJECTIVE: To investigate the RBC immune functions in childhood idiopathic thrombocytopenic purpura (ITP). METHODS: RBC-CR1 polymorphism genotype, RBC immune function and its regulator function were detected in 22 ITP, 30 allergic purpura (AP) and 50 normal children by PCR and Guo Feng's method. RESULTS: 1. There were 18 cases of high expression (82%) and 4 of medium expression (18%) of RBC-CR1 genotypes in the ITP cases. The rate of the medium expression genotype was significantly higher in ITP than that in AP and normal children (P < 0.01). 2. The rates of RBC-C3bRR and RBC-C3bRFER were much lower (P < 0.01), and the rates of RBC-ICR and RBC-C3bRFIR were significantly higher in ITP than those in normal children (P < 0.01). 3. RBC-CR1 genotypes in ITP were correlated negatively with RBC-C3bRR and RBC-C3bRFER (P < 0.05), but positively with RBC-ICR and RBC-C3bRFIR (P < 0.05). CONCLUSION: RBC immune participated in the pathogenesis of ITP.
Child↗
[Expression and purification of recombinant hirudin].
OBJECTIVE: To express the recombinant hirudin variant 1 (rHV1) with biological activity in prokaryotic cells and then isolate and purify the expressed products. METHODS: The hirudin variant 1 gene in plasmid vector pBV220 was expressed in E. coli strain DH5 alpha. The biological activity of rHV1 was determined with chromogenic substrate method. The expressed product was purified by ultrafiltration, DEAE-Sephadex A-50 filtration and thrombin-Sepharose 4B affinity chromatography. RESULTS: The expressed rHV1 accounted for approximately 16.9% of E. coli cell proteins with an activity of 20-30 ATU (antithrombin unit) per milliliter culture. The purified rHV1 showed a homogeneous band on SDS-PAGE. CONCLUSIONS: This is the first report in China on successful expression of hirudin variant 1 gene in E. coli and purification of the expressed rHV1.
Escherichia coli↗
[Advances in the molecular biological study of hereditary antithrombin III deficiency].
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Antithrombin III↗
[Investigation of the clinicopathologic features, immunophenotype and Epstein-Barr virus infection of the upper respiratory tract lymphomas in patients].
OBJECTIVE: To study the clinicopathologic features, immunophenotype and Epstein-Barr virus (EBV) infection of the upper respiratory tract lymphomas in patients from north China. METHODS: 112 cases of upper respiratory tract lymphomas in patients from north China were investigated. HE stain for histologic features. Immunohistochemistry studies were performed using monoclonal antibodies, UCHL-1 for T-lymphocyte, L26 and 4KB5 for B lymphocyte, CS1-4 for latent membrane protein-1 (LMP-1). In situ hybridization for EBV encoded small nuclear RNA (EBER 1/2, Y017, DAKO). RESULTS: 77/112 cases (68.75%) were T-cell lymphomas, 35 cases (31.25%) were B cell lymphomas. EBV-EBER 1/2 in situ hybridization positive in 54/101 (53.46%) cases, including 48 T-cell lymphoma cases and 6 B-cell lymphoma cases. CONCLUSION: Most upper respiratory tract lymphoma cases in patients from north China are T-cell lymphomas and are strongly associated with EBV.
Adolescent↗
Opening blood-brain-barrier by intracarotid infusion of papaverine in treatment of malignant cerebral glioma.
OBJECTIVE: To find an effective method for the treatment of malignant cerebral glioma by opening the blood-brain-barrier (BBB). METHODS: The concentration of methotrexate (MTX) in tumor was measured in 18 patients with cerebral glioma, and 155 patients with malignant glioma were treated by intracarotid infusion of papaverine and Bis-chloronitrosourea (BCNU). RESULTS: After intracarotid infusion of papaverine for reversible opening of BBB, the MTX concentration in tumor tissue was 1810 +/- 380 ng/g, higher than that in patients without papaverine infusion (997 +/- 126 ng/g). Eighty-nine patients with glioblastoma and 66 patients with anaplastic astrocytoma received intracarotid infusion of papaverine and BCNU (250 mg) for an average of 2.14 times. The mean survival time of these patients was 114.5 weeks, median survival time was 140 weeks, and five-year survival rate was 22.97%. CONCLUSION: The combined chemotherapy with papaverine and BCNU through intra-arterial infusion is simple, safe and effective in treating malignant cerebral glioma so long as the tumor is sensitive to the drugs.
Adolescent↗
[A study on DPOAE in patients with diabetes mellitus].
In present experiment we tested DPOAE in 19 patients with diabetes mellitus and compared with control subjects. Results showed that DPOAE (f2 above 3 kHz) disappeared in 2 patients (3 ears) indicating cochlear lesion, both of DPOAE contralateral suppressive effects and selective attention effects significantly reduced in 4 patients (7 ears), and reversed in 3 patients (6 ears) indicating disorders existing in cortex topdown and efferent of MOCS. It was suggested that tests of DPOAE and suppression (induced by CAS and selective attention) may be useful for making early diagnosis of cochlear-pathy and auditory efferent disorders (cortex and MOCS) before retinalpathy happened. This study on DPOAE provided important evidence for early diagnosis and early treatment of diabetic complications.
Acoustic Stimulation↗
[Application of coronal CT scan and three-dimensional reconstruction in rhinology].
To display the structure of the Ostiomeatal Complex (OMC) more clearly, and provide clearer and more living imaging for safe functional endoscopic sinus operation and thoroughly eliminating the focus of disease, coronal CT scanning and 3-dimensional reconstruction were performed in 168 sinusitis patients (290 sides) and 107 patients with sinusitis and nasal polyposis (175 sides). The CT findings were compared with endoscopic examination and normal people were taken as control. The found of coronal CT and endoscopic examination are consistent in recognizing mucosal thickening within nasal cavity and sinuses. Three-dimensional CT is more living than common coronal CT in displaying the uncinate process and turbinates. Our conclusion is that coronal CT scan processes high values for recognizing nasal sinus disease, anatomical variants, disease extent and adjacent structures of OMC. Three-dimensional reconstructed CT make the image of OMC more stereoscopic, audio-visual and living.
Adult↗
[Effects of testing parameters on the result of tympanometry].
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Acoustic Impedance Tests↗