Biomedical subjects

H Wang

Publications and source records attributed to H Wang.

At least 595 records · Page 33Linked to original sources

PubMed2001

[Clinical significance of hemostatic molecular markers in acute leukemia].

OBJECTIVE: To study the changes of hemostatic molecular markers in acute leukemia (AL) for elucidating their clinical significance. METHODS: A series of hemostatic molecular markers, including TF, TFPI, TAT, PAP, u-PA and u-PAR were measured in 82 acute leukemia. RESULTS: The plasma levels of TF, TAT, u-PA and u-PAR were elevated significantly at diagnosis in all AL patients tested, while TFPI, PAP did only in AML. After treatment, TF and TAT remained high in AML, u-PA and u-PAR were still high in un-remission patients. PAP and u-PA remarkably elevated in patients with severe hemorrhage. CONCLUSIONS: There existed hemostatic abnormality as well as hyperfibrinolysis, which varied with leukemia types and was ameliorated with clinical improvement. The measurement of TF, TAT and PAP may provide useful information for the diagnosis of DIC. u-PA and u-PAR may be considered as a useful indicator for prognosis. Patient with severe hemorrhage should be treated with antifibrinolysis drugs. The prevention of hypercoagulability after treatment should be kept in mind.

Acute Disease↗

PubMed2001

[Selection of optimal ultrasonic extraction process of Elaeagnus angustifolia L. by uniform design].

The influences of ultrasonic frequency, ultrasonic time and solvent content on the extraction rate of fat oil in Elaeagnus angustifolia L. were studied. The optimum extraction condition was obtained. The results showed that the ultrasonic extraction method of fat oil in Elaeagnus angustifolia L. could save time, improve extraction rate and need not be hot compared with traditional extraction method.

Elaeagnaceae↗

PubMed2001

[A clinical study of haploidentical transplantation using granulocyte colony-stimulating factor stimulating donor bone marrow].

OBJECTIVE: To explore the effects of reducing the incidence of severe acute graft-versus-host disease (GVHD) and improving the disease free survival(DFS) in haploidentical donor transplantation by granlocyte colony-stimulating factor (G-CSF) administration to donor before harvesting and a number of immunosuppresants added to host. METHODS: Thirteen patients with leukemia received allo-bone marrow transplantation (BMT) from two or three HLA loci mismatched related donor (haploidentical group). The clinical outcomes of the bone marrow transplantion were compared with thase of 13 consecutive HLA identical sibling transplantion (identical group). In haploidentical donor BMT, the donors of patients were given G-CSF (Lenograstim Chugai) 250 micrograms/day for seven doses prior to marrow harvest. CSA, MTX, ATG and mycophenolate mofetil (MMF) were combined for GVHD prophylaxis. ATG 5 mg/kg/day was infused for 4 days before transplantation and MMF was adminisered from 7th day after. RESULTS: All the patients were engrafted. The median number of CD34+ cells in graft was 6.1 x 10(6)/kg in haploidentical group and 2.5 x 10(6)/kg in identical group (P < 0.01). The median number of CD3+ cells was 50.5 x 10(6)/kg and 47.0 x 10(6)/kg respectively (P > 0.05). All patients had 100% donors hematopoietic cells after transplantation by cytogenetic evidence analysis. Five of the thirteen patients (38.5%) in haploidentical group and three of the thirteen patients(23.1%) in identical group experienced II-IV acute GVHD (P > 0.05). The probability of chronic GVHD was 87.5% in haploidentical group and 67.5% in identical group (P > 0.05), However none in both groups developed extensive cGVHD. The median follow-up duration was 453 days (range 180-690 days) for haploidentical group and 510 days (range 220-810 days) for identical group. In haploidentical group, five patients died from transplant related mortality (3 GVHD, 2 infection), none relapsed and eight patients(61.5%) survive in disease free situation. In identical group, two patients died from transplant related mortality (1 GVHD, 1 infection), two patients died from relapse and nine patients (69.2%) survive in disease free situation. DFS in haploidentical group and in identical group was similar(P > 0.05). CONCLUSION: The transplants from haploidentical donor used in this study is 3 effective and feasible in preventing acute severe GVHD and improving DFS.

Adolescent↗

PubMed2001

[Isolation of HIV-1 from infected individuals in Fujian Province].

OBJECTIVE: To isolate the virus from HIV-1 infected individuals. METHODS: Blood specimens were collected from 10 HIV-1 infected persons in Fujian Province. Peripheral blood mononuclear cells (PBMCs) were separated from the heparinized blood. Each sample was cocultured with fresh normal PBMCs to isolate HIV-1. Supernatant of the cocultures was tested by using HIV-1 P24 antigen Kit. RESULTS: Of the 10 specimens, 9 showed positive for the P24. 4 of 9 isolated infected MT4 cells, and cell syncytias could be seen. Three isolates showed transient infection of MT4, which probably belong to slow/low virus. Only one isolate could infect MT4 successively, which had been passed for 15 generations without significantly changed viral titer; it is believed to be a rapid/high replication virus. CONCLUSIONS: HIV-1 virus was successfully isolated.

HIV Infections↗

PubMed2001

[Analyzing partial nucleotide and amino acid sequences of Chinese TTV isolates].

BACKGROUND: To investigate the prevalence of transfusion transmitted virus (TTV) and its sequence characterization in China. METHODS: TTV DNA was amplified by Semi-Nested-PCR, and the positive products were sequenced directly. RESULTS: The homology of partial nucleotide and amino acid sequences of 7 Chinese TTV isolates were 64.7%-98.4% and 62.7%-96.4% respectively when compared with Japanese isolate. Among 7 Chinese TTV isolates, the homology of partial nucleotide and amino acid sequences were 63.5%-98.4% and 60.2%-96.4% respectively. They differently belong to two genotypes and two subgenotypes. CONCLUSIONS: There exists TTV in China, and there might be several genotypes of Chinese TTV isolates.

Amino Acid Sequence↗

PubMed2001

[Serum glutathione S-transferase in bromobenzene-induced acute hepato-toxicity in mice].

The activity of serum glutathione S-transferase (GST) was tested in bromobenzene-induced acute hepatotoxicity in mice. The data showed that a lot of small local necrosis in liver biopsy was observed and serum GST activity was increased after 2 h of treatment with bromobenzene (150 mg/kg i.p.) in mice. Serum alanine transaminase (ALT) activity was also gradually enhanced after 8 hours of treatment. The most obvious changes were at 16 hours of bromobenzene treatment, and serum GST activity was firstly restored to normal level after 24 hours of treatment. There were good positive correlations between serum GST, ALT, as well as serum GST and the changes on liver biopsy. Evaluating analysis of the diagnosis test showed that the sensitivity, specificity and accuracy was 89%, 75% and 82% in serum GST test, and 39%, 92% and 64% in serum ALT test respectively. The ROC work curve also demonstrated that serum GST was more sensitive and specific than serum ALT.

Alanine Transaminase↗

PubMed2001

[Study on expression of Fas/FasL and HBV antigens in liver tissue of patients with hepatitis B].

OBJECTIVE: To evaluate the role of apoptosis mediated by Fas/FasL in the liver of hepatitis B and the expression status of Fas, FasL, and HBV antigens. METHODS: We studied the expression of Fas antigen, Fas-ligand (FasL) and hepatitis B virus(HBV) antigens(HBsAg and HBcAg) in the livers of 62 patients with hepatitis B using immunohistochemistry ABC method. Six normal liver samples were used as control. RESULTS: Hepatocytes in normal liver had no Fas/FasL expression, but in the cases of hepatitis B, Fas was expressed mainly in the cytoplasm in 58 cases(93.5%); FasL was observed in infiltrating mononuclear cells and in the cytoplasm of hepatocytes in 37 cases (59.7%). The expressions of Fas/FasL were closely related to the liver histological inflammation degrees. CONCLUSION: The apoptosis mediated by Fas/FasL may play an important role in the hepatocellular injury in hepatitis B. No correlation was found between the degrees of Fas/FasL expression and the presence of HBsAg and HBcAg in the liver.

Adolescent↗

PubMed2001

[Detection of TTV DNA and TTV IgG in 328 serum samples].

OBJECTIVE: To investigate the prevalence of TT virus(TTV) in a variety of populations in China. METHODS: TTV DNA and anti TTV IgG were simultaneously tested by PCR and ELISA. RESULTS: The positive rate of TTV DNA in the samples of 81 normal subjects, 92 blood donors, 123 hepatitis A-G patients and 32 hepatitis non A-G patients were 2.5%, 2.2%, 19.5% and 28.1%, respectively; and anti-TTV IgG was 1.2%, 3.7%, 26.8% and 34.4%, respectively. There were significant differences between the normal and the blood donor group and the hepatitis group; and among multi-infectious cases, the infection rate of TTV with HBV was as high as 75.0%. CONCLUSION: The investigation indicated that TTV infection was very popular in different groups. Some of the normal and blood donor groups infected with TTV could become healthy carries of virus. And the hepatitis patients were the high risk group. In addition, TTV may be transmitted via other pathways besides blood.

Adult↗

PubMed2001

[Determination of shrinkage and bending strength of GI-II infiltrated ceramic].

OBJECTIVE: To determine the shrinkage and strength of GI-II Infiltrated Ceramic for providing scientific basis of its clinical use. METHODS: The adobes by slip-casting alumina powder of GI-II Infiltrated Ceramic were sintered at 1125 degrees C for 2 hours. The sintered alumina bodies were then divided into two groups and infiltrated by melt glass at 1125 degrees C and 1100 degrees C respectively for 6 hours. The length of the infiltrated ceramic specimens before and after fabrication was determined to calculate shrinkage. Their strength values were obtained by 3-point bending test. RESULTS: The infiltrated ceramic infiltrated at 1125 degrees C was 0.289 +/- 0.155% in shrinkage, 367.7 +/- 76.1 MPa in strength, and 4.79 in Weibull modulus; that infiltrated at 1100 degrees C was 0.253 +/- 0.043%, 377.5 +/- 64.4 MPa, and 5.52, respectively. There was no statistically significant difference between those two groups. But their strength values were statistically high, compared with 300 MPa. CONCLUSION: The shrinkage of GI-II Infiltrated Ceramic could be compensated by brushing spacer on dies and expansion of die stone. Its strength satisfied the requirement for clinical use of all-ceramic bridge materials. This kind of high-strength and low-shrinkage ceramic could be used for fabricating all ceramic crown and bridge core.

Aluminum Oxide↗

PubMed2001

[Research on the high strength all ceramic crown by the combination of CAD/CAM and glass infiltrated alumina blocks].

OBJECTIVE: To search for a method that fabricates the high strength all ceramic crown by the combination of CAD/CAM and glass-infiltrated alumina. METHODS: Alumina coping was milled by the Cerec II CAD/CAM and glass was infiltrated into alumina by the capillary effect. Vita alpha ceramic was used to veneer the surface of substructure for all ceramic crown. RESULTS: This technology need not duplicate die and sinter alumina substructure for 10 h, compared with the conventional In-ceram technology. The time of glass infiltration has been decreased from 4 h to 40 minutes by the high capillary effects of prefabricated alumina blocks. CONCLUSION: This is a new method for all ceramic crown by using Cerec II CAD/CAM to mill a simple ceramic block so as to fabricate prosthetics in clinic.

Aluminum Oxide↗

PubMed2001

[Expression of type I collagen mRNA in rat molar periodontal ligament under normal bite force].

OBJECTIVE: The main component of the periodontal ligament(PDL) is collagen fiber, especially type I collagen, and collagen plays an important role in PDL. The aim of this study is to observe the expression of rat PDL mRNA under normal bite force and to investigate the molecular mechanisms of these changes in type I collagen mRNA. METHODS: 40 male Wistar rats were used, and animals were intra-cardiac perfused with a solution of 4% polyformaldehyde under anesthesia. Dissected mandibles were immersed in the same fixation for 6 hours and subsequently decalcified in EDTA. The demineralized specimens were embedded in paraffin and cut into slices with thickness 5 microns. The probe was synthesized and labeled with digoxigenin. Expression of type I collagen mRNA was measured by using in situ hybridization(ISH). RESULTS: Under the normal bite force, the mRNA expression of type I collagen was very strong on the whole, including the alveolar bone side, the root side and the area between them. Positive signals were located mainly in the cytoplasm and some in the nuclei. But the mRNA expression of type I collagen still had spatial characteristics. The signals in some fibroblasts were apparently stronger than those in other fibroblasts in the apical 1/3 fragment of the roots. The signal of type I collagen mRNA was strong near the root sides. The expression signal on the proximal alveolar walls was strong, however, on the distal alveolar wall, there was no expression. CONCLUSION: The expression of type I collagen mRNA is closely related with bite force.

Animals↗

PubMed2001

[Study of bacteriology on local application of Tinidazole stilus in treatment for periodontitis].

OBJECTIVE: The study was designed to evaluate the antiseptic efficacy of Tinidazole stilus against periodontal pathogen. METHODS: A total of 41 patients with periodontitis were included in this study. 26 of them were treated by Tindazole stilus and other 15 cases were treated by metronidazole stilus as controls. The prevalence of subgingival bacteria from both group were examined. RESULTS: The eliminate rates for Black pigmented anaerobic rod (Porphyromonas gingivalis, Prevotella intermedius, Prevotella melaninogenicus), Fusobacteriums, Prevotella oris, Prevotella oralis, Eubacterium and Actinomyces were 95.8% 94.1%, 83.3% and 76.5%. The anti-bacterial efficiency of Tinidazole against periodontal pathogen was significantly higher than that of metronidazole (P < 0.05). CONCLUSION: It appears to be effective to use Tinidazole stilus as local application against periodontal pathogens.

Administration, Topical↗

PubMed2001

[Shear strength of bond of the dentin to GI-II glass-infiltrated alumia ceramic agter varying modes of surface conditioning].

OBJECTIVE: The adequate surface conditioning method for GI-II glass infiltrated alumia ceramic was investigated. METHODS: The test specimens were divided into 3 groups (A. HF + silicon, B. sandblasting + silicone and C. thermal silicoating + silicone). Shear bonding strengths of porcelain bonded to dentin were tested. RESULTS: The bond strength of group A and B slightly increased after one day but decreased sharply after 30 days. However, no statistical significance was observed. The group C showed a pronounced enlargement of bond strength after one day and had a durable bond after 30 days' thermal cycling. It is statistically significant when group C was compared with group A and B. CONCLUSION: It can be inferred that silicoating method be a better way for glass infiltrated alumia ceramic bonding.

Aluminum Oxide↗

PubMed2001

[Application of posterior canal wall reconstruction and mastoid obliteration].

OBJECTIVE: To explore the obliteration of the old mastoid cavity and reconstruction of the posterior canal wall after radical mastoidectomy. METHOD: Posterior canal wall reconstruction and mastoid obliteration of 11 ears of 10 patients with old mastoid cavity for 1 to 6 years after radical mastoidectomy were done using the pedicle postauricular composite skin flap (PPCSF), and their tympanoplasty were performed at the same time. Follow-up was performed at 3 to 30 months. RESULT: All PPCSFs survived. The old mastoid cavity of 11 ears vanished and reconstructed posterior canal wall remained in normal position without retraction. The drying ear time took 2 to 3 weeks postoperatively, and the symptoms of the radical cavity disease after radical mastoidectomy were cleared up nearly, and the average hearing threshold decreased 13.6 dB HL. The otorrhea and perforation of tympanic membrane resulted from infection happened to 1 of 11 ears three month after the operation. CONCLUSION: The PPCSF is an effective method in the obliteration of old mastoid cavity and reconstruction of the posterior canal wall. It can reduce drying ear time postoperatively, treat the radical cavity disease after radical mastoidectomy and is beneficial to the tympanoplasty at the posterior external at the same time.

Adolescent↗

PubMed2001

[Study on the working noise in BYPC and the effects caused by working noise on the workers' vestibular and auditory function].

OBJECTIVE: In order to observe the kinds and intensity of the working noise of Yansan Petrochemical Co. and the effects caused by the working noise on the workers' vestibular and auditory function. METHOD: The intensity and frequency of the working noise were recorded by exactolnoisemeter in the workshop. One hundred and seventeen workers were tested in routine pure-tone tested method. The SPVN and ABR were tested within fifty-one workers of all. RESULT: The working noise of Yansan Petrochemical Co. belongs to the broad band and steady noise. The intensity of the working noise were during 85.7-104.0 dB(A) and the main frequency were during 1-8 kHz. About 59 percent workers who exposed to the working noise had hearing loss. The most hearing-loss were in the high frequency. The hearing-loss of speech frequency were slight. Workers who have more six years standing have obviously increased hearing-loss than the workers who have less five years standing. There were significant differences the ABR thresholds and wave-interval between the tested and controlled groups. The SPVN and CP were abnormal in more than 17.4 percent workers with hearing loss. CONCLUSION: The working noise of Yansan Petrochemical Co. belongs to the broad band and steady noise. Working noise can lead to workers' hearing loss of certain degrees who exposed in the noise for a long time. Obvious correlation was not defined between the hearing-loss and the abnormal vestibular response group.

Adult↗

PubMed2001

[The dynamic study of cytokines pre- and postoperation in patients with carcinoma of larynx].

OBJECTIVE: To observe the dynamic changes of cytokines pre- and postoperation in patients with carcinoma of the larynx and provide reference for recurrence monitor and prognosis evaluation in postoperative patients with head and neck tumor. METHOD: Thirty-four patients with head and neck squamous cell carcinoma were included in this study. Serum levels of IL-2, sIL-2R and TNF-alpha were measured before surgery, 7 days, 30 days and 90 days after surgery with ELISA and RIA. RESULT: The serum levels of sIL-2R and TNF-alpha before surgery in patients with carcinoma of the larynx were significantly elevated compared with controls(P < 0.01), while the level of IL-2 was not different. Serum concentrations of sIL-2R and TNF-alpha in patients without recurrence found at 7 days after operation were slightly higher than those before operation, but there was not significant difference. Moreover, the levels of sIL-2R and TNF-alpha at 30 days and 90 days postoperation decreased gradually, especially the sIL-2R levels decreased more significantly. No statistical difference were found among serum IL-2 levels in non-recurrence patients. The serum levels of sIL-2R and TNF-alpha in patients with recurrence at 30 days after operation were lower than those before operation and increased at 90 days after operation, while the levels of IL-2 decreased gradually. CONCLUSION: Dynamic study of the cytokines pre and postoperation play reference role in recurrence monitor and prognosis evaluation after the larynx carcinoma were removed.

Adult↗

PubMed2001

[Surgical treatment and reconstruction for the patients with advanced-stage tonsillar cancer].

OBJECTIVE: To investigate the operative approach and reconstruction for the patients with advanced-stage tonsillar cancer. METHOD: 7 cases with advanced-stage tonsillar cancer were operated from Dec. 1994 to May 1999. Among them, 5 cases were operated through combined neck-mandible-oral cavity approach (CNMOCA) and with immediate reconstruction. Forearm free skin (FFS) flaps were applied in 3 cases. Pectoralis major myocutaneous (PMM) flaps were applied in 2 cases. 2 other cases were operated separately through approach of oral cavity or lateral pharynx. 6 cases were operated with radical neck dissection. RESULT: 5 cases who were operated through CNMOCA or with reconstruction were followed up from 1 year to 5 years and 3 months. They had not recurrence of tumor and complication. All had function recovery of speech. Swallowing or appearance is satisfactory in the 3 cases with FFS flaps. 2 Other cases died as recurrence of tumor in 1 year and 2 years and 10 months post-operation. CONCLUSION: CNMOCA is able to provide a good exposure method to remove the extensive tumors completely for the patients with advanced stage tonsillar cancer. It is necessary to reconstruct immediately for the patients.

Adult↗

PubMed2001

[The effects of BDP on the inflammatory cells in nasal secretions of the patients with allergic rhinitis].

OBJECTIVE: To study the effects of beclomethasone dipropionate (BDP) on the inflammatory cells in nasal secretions of the patients with allergic rhinitis. METHOD: The variation of intercellular RNA and RNA/DNA ratio of the eosinophils, neutrocyte and lymphocyte in the nasal secretions were observed under the laser scanning confocal microscopy and technology of fluorescence. RESULT: The scanning images displayed that either in eosinophils or in neutrocyte, the fluorescence signal of intercellular RNA was significantly decreased after BDP treatment, and the intercellular RNA/DNA ratio was significantly reduced (eosinophils group P < 0.001, and neutrocyte group P < 0.01). The fluorescence singal of lymphocyte intercellular RNA and RNA/DNA ratio were not significantly changed (P > 0.05). CONCLUSION: BDP can reduce the content of intercellular RNA of the eosinophils and neutrocyte. The anti-inflammation function of BDP is realized by regulating the intercellular RNA synthesize and/or RNA degradation, and by inhibiting the intercellular inflammatory medium production. BDP does not directly influence the intercellular RNA metabolize of the lymphocyte.

Adult↗