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Biomedical subjects

H Wang

Publications and source records attributed to H Wang.

At least 613 records · Page 34Linked to original sources
PubMed2001

[Synthesis and evaluation of ethylstyrene-divinylbenzene-coated titania for high performance liquid chromatography].

Ethylstyrene-divinylbenzene-coated TiO2 (ES-DVB-TiO2) was synthesized by at first bonding of vinyltriethoxysilane with hydroxyl group on titania and secondly by addition polymerization of vinyltriethoxysilane and ethylstyrene-divinylbenzene. The hydrophobic selectivity, absolute mobile phase sensitivity, and stability of the stationary phase were evaluated. Finally, several kinds of practical separations were tested on ES-DVB-TiO2. These examples showed that ES-DVB-TiO2 synthesized in our laboratory was a good reversed-phase stationary phase.

Benzene Derivatives↗
PubMed2001

[Cloning and sequencing of Chinese squash leaf curl virus].

Sequence analysis of virus isolation DNA of Squash leaf curl disease shows that Squash Leaf Curl Virus (SLCH-CHI) contains 2741nt, which encode six Open Reading Frames (ORFs). Two of them are on virus sense strand: AV1 (256aa) and AV2 (140aa). AV1 encode coat protein. The other of ORFs are on complementary sense strand: AC1 (replicase gene, 243aa), AC2 (transactivtor, 134aa), AC3 (136aa) and AC4 (172aa). The virus belongs to one number of begomoviruses from old world.

Amino Acid Sequence↗
PubMed2001

[Gene cloning and expression of human bactericidal/permeability-increasing protein].

The gene of human bactericidal/permeability-increasing protein (BPI) was cloned from peripheral blood lymphocytes of a normal Chinese individual. The result of sequencing showed the gene is 1452 bp encoding a 27-residue signal peptide and a 456-residue matured protein, and it has six nucleotide variations compared with the sequence reported which results in 4 different amino acids. In order to get recombinant BPI, the gene was cloned into an expressing plasmid and expressed in CHO cells. The recombinant protein was purified using cation-exchange chromatography and its bioactivity was proved with bactericidal assays.

Amino Acid Sequence↗
PubMed2001

[Investigation and evaluation on the harmfulness of partial vibration and the contact vibration time].

The harmfulness from partial vibration with extended contact vibration time was studied by investigating into the symptoms and signs of workers with local vibration disease in Taiyuan Iron & Steel Company. The sense of hand, the function of nerve ending and the microcirculation of fingernail wrinkles of 321 workers working on local vibration operations were examined and observed. The indexes examined are worse with the increase of contact time and are better in low contact group. A positive correlation between the abnormal of microcirculation, the pain and numbness of hand was observed. It is concluded that the degree of harmfulness on hands and upper limbs of whom working on local vibration operation is related to the time of contact vibration. The function of nerve ending and the ending circulation were injured at the same time.

Adult↗
PubMed2001

[Effects of air pollution from burning coal on respiratory diseases in adults].

A cross sectional epidemiological study on the effect of air pollution from burning coal on the occurrence of respiratory symptoms and diseases in adults was carried out in Taiyuan city of Shanxi province. Adult residents above 25 year-old living in three areas with different degrees of pollution were selected. The results showed that the prevalence of respiratory symptoms and diseases increased with the extent of air pollution after age, sex, occupation, smoking, indoor coal-burning and history of diseases were adjusted with Logistic model.

Adult↗
PubMed2001

[Cloning and sequence analysis of the phytase phyA gene of Aspergillus niger N25].

The phyA encoding phytase of Aspergillus niger N25 was amplified by the polymerase chain reaction (PCR) with primers designed according to the sequences of the phyA in GenBank. The amplified fragment was cloned and sequenced. The results show that: the coding region is 1506 bp in size, includes a 102 bp intron, and encodes a peptide of 476 amino acid residues, in which there is a signal peptide with 19 amino acids and a mature peptide of 448 amino acids. Comparison of this sequence with the phyA of the natural A. niger NRRL3135 (GenBank Accession: M94550), the most highly secreting-phytase strain, shows that the nucleotide homology is as high as 96.746%, and the amino acid homology comes up to 97.64%. The phyA of A. niger N25 strain in this paper is appropriate to be used to construct the phytase gene-engineering bacteria.

6-Phytase↗
PubMed2001

[Clinical observation of effect on tongmai yizhi capsule in treating vascular dementia].

OBJECTIVE: To observe the effect of Tongmai Yizhi capsule (TMYZC) on learning in patients of vascular dementia (VD). METHODS: Thirty patients of VD were treated by oral administration of TMYZC, the changes in them before and after treatment were observed and compared with those in the 20 patients treated with hydergine as control. RESULTS: The total effective rate in the TMYZC treated group was 70.67% while that in the control group was 70.00%, the difference between the two groups was insignificant. CONCLUSION: Both TMYZC and hydergine have the effects of dilating blood vessels, increasing the cerebral blood flow, lowering the hyper-coagulative status and improving the free radical scavenging capability of patients.

Aged↗
PubMed2001

[Preliminary experimental study on therapeutic mechanism of kangzhen zhijing capsule in treating Parkinson's disease].

OBJECTIVE: To explore the therapeutic mechanism of Kangzhen Zhijing Capsule (KZZJC) on rats with Parkinson's disease (PD). METHODS: PD rat model was established by microinjection of 6-hydroxydopamine solution into left substantia nigra zona compacta (SNZC). Model rats were randomly divided into 3 groups and treated with KZZJC, levodopa and normal saline respectively for 30 days. The rotational behavior of rats before and after treatment was observed. Then rats were sacrificed and relevant indexes of left SNZC and striatum were examined. RESULTS: In rats treated with KZZJC, the rotational behavior was improved (P < 0.05), the levels of dopamine (DA) content and DA/homovanillic acid (HVA) ratio in striatum, and glutathione peroxidase, superoxide dismutase and glutathione in SNZC increased, but malondialdehyde in SNZC decreased, as compared with those in rats treated with normal saline, the difference was significant (P < 0.01 or P < 0.05). Rotational behavior in rats treated by levodopa was improved significantly (P < 0.01). CONCLUSION: KZZJC could partially improve the rotational behavior in PD model rats, increase DA content and DA/HVA ratio in striatum. The mechanism might be the reducing of free radical damage and DA catabolism.

Animals↗
PubMed2001

[Synthesis and biological activities of new 5-HT2A selective ligands--N-substituted-piperidinyl-4-phenylthioether and sulfone derivatives].

AIM: A series of 4-piperidinylthioether and sulfone derivatives of 4-[1-hydroxy-1-(2,3-dimethoxyphenyl) methyl]-N-2-(4-fluorophenylethyl) piperidine (MDL 100907) were synthesized in order to find new 5-HT2A selective ligands. METHODS: Title compounds 2a-2c were synthesized from 2, 3-dimethoxythiophenol and tested for their affinities to 5-HT2A, 5-HT2C, 5-HT6 and 5-HT7 receptors and some other nervous transmitter receptors in vitro. RESULTS: Compounds 2a-2c are new compounds. The results of the binding assay demonstrated that they have relatively high selectivity for 5-HT2A receptor in vitro. CONCLUSION: Some sulfur containing analogues of MDL 100907 showed selective affinity to 5-HT2A receptor and are worth further study.

Animals↗
PubMed2001

[Study on the permanent MRI technology].

This paper describes the open permanent MRI technology, introduces the special technology such as main magnet field, gradient magnetic field coil, Transmitter/Receiver coils and so on, and points out that the installation and adjustment of the MRI are also very important for optimal operation and best image quality.

Electromagnetic Fields↗
PubMed2001

[Characterization of alumina adobe and sintered body of GI-infiltrated ceramic].

OBJECTIVE: This study was conducted to elucidate the mechanism of formation of porous structure by investigating the porosity of the alumina adobe and sintered body of GI-II Infiltrate Ceramic, and its role in strengthening and toughening this kind of ceramic composite. METHODS: The alumina powder size-mass distribution was obtained by BI-XDC powder size analysis device; the open pore parameters of alumina adobe and sintered body were analyzed using the mercury pressure method. Their fracture surfaces were observed under scanning electronic microscope. RESULTS: Fine powder had two main size groups of 0.09-0.1 micron and 0.2-0.5 micron, respectively, and coarse powder, with size between 1.5 to 4.5 microns, occupied the majority of powder mass. Alumina adobe's pores became larger after sintering. The median pore radii of adobe and sintered body were 0.2531 micron and 0.3081 micron, respectively; the average pore radii changed from 0.0956 micron to 0.1102 micron. Under scanning electronic microscope, fine alumina powders were fused partially together and their surfaces were blunted, but coarse powders did not show such phenomena. CONCLUSION: The alumina size distribution contributes to the formation of porous structure of alumina sintered body. This porous structure is not only the shape skeleton but also the mechanical skeleton of GI-II Infiltrated Ceramic. It plays an important role in raising the mechanical properties of this kind of ceramic composite.

Aluminum Oxide↗
PubMed2001

[Effect of decreased bite force on the temporal and spatial expression of type I collagen mRNA in rat molar periodontal ligament].

OBJECTIVE: The aim of this study was to observe the effect of decreased bite force on type I collagen mRNA in periodontal ligament (PDL) and probe into the molecular mechanism of the change in type I collagen mRNA. METHODS: Animal models were established by extracting left maxillary molars of rats. In situ hybridization was applied. RESULTS: The results showed that under decreased bite force, the expression level of type I collagen mRNA decreased at the sixth hour (49.7 +/- 11.1) (P < 0.05), reached the nadir on the second day (23.7 +/- 8.6), began to get over on the third day (43.1 +/- 10.3), and reached a relatively high level at the second week(56.3 +/- 9.8), but it was still lower than that of the normal bite force group (91.4 +/- 16.0)(P < 0.05); the expression of type I collagen mRNA near the cementum was higher than that near the alveolar bone. CONCLUSION: These results indicate that the expression level of type I collagen mRNA is closely related to bite force.

Animals↗
PubMed2001

[Effect of increased and decreased bite force on morphology of periodontal tissues].

OBJECTIVE: The aim of this study was to observe the effect of bite force on morphology of periodontal tissues. METHODS: Animal models were induced by extracting the left maxillary molars; the left mandibular molars served as decreased bite force models; the right mandibular molars served as increased bite force models. The dynamic changes of widths of periodontal ligament and cementum were measured. RESULTS: In decreased bite force group, there were significant morphologic changes of periodontal tissues after 1 week. In increased bite force group, there was no significant change. Ligament widths were significantly different after 2, 3 weeks respectively in those two groups, compared with that in control group (P < 0.05). There were significant differences between proximal alveolar wall and distal alveolar wall in width (P < 0.05). CONCLUSION: The histological morphology is closely related to the mechanical condition. Physiological bite force is necessary for periodontal ligament to maintain its physiological structure.

Animals↗
PubMed2001

[A clinical evaluation on reconstruction in extended partial laryngectomy for transglottic cancer].

OBJECTIVE: Transglottic cancer has been a definite indication for total laryngectomy in decades, compromising function of the larynx. The purpose of this study is to present a new radical surgery of extended partial laryngectomy and reconstruction to restore the essential function of larynx. METHODS: Forty-one cases of transglottic cancer were treated by extended vertical laryngectomy (26/41), extended frontolateral laryngectomy (5/41) and subtotal laryngectomy (10/41); the defects of larynx were reconstructed by rotary door myocutaneous flap in twenty-seven cases, by sternohyoideus flap in seven cases, by osteomuscular flap in five cases and by sternocleidomastoid flap in two cases. RESULTS: The 3 and 5 year survival rates were 85.7% (30/35) and 74.1% (20/27) respectively, those for stage III were 84.6% (22/26) and 76.2% (16/21), for stage IV were 3/4 and 1/2 respectively. All cases resumed acceptable voice, 92.7% (38/41) and enjoyed satisfactory phonation. The over-all decannulation rate was 87.8% (36/41), those with rotary door myocutaneous flap was 96.3% (26/27), with sternohyoideus flap was 5/7, with osteomuscular flap was 4/5 and with sternocleidomastoid flap was 1/2. All patients had normal swallow function. CONCLUSION: Extended partial laryngectomy is a kind of radical operation in selected cases of transglottic cancer. The method of reconstruction with rotary door myocutaneous flap can improve curative effect and the essential functions of the larynx.

Adult↗
PubMed2001

[Clinical evaluation on second stage reconstruction for laryngostenosis after partial laryngectomy for laryngeal cancer patients].

OBJECTIVE: To present a new method of decannulation for laryngostenosis patients after partial laryngectomy and extended partial laryngectomy, and restoration of the essential functions of larynx and normal neck appearance. METHODS: Nineteen cases of laryngostenosis after partial laryngectomy and extended partial laryngectomy were treated with second stage reconstruction. The patients had been treated by vertical laryngectomy (6/19) using extended vertical laryngectomy (11/19) and frontolateral laryngectomy (2/19). Among these patients, second stage reconstruction of larynx was performed by using rotary door myocutaneous flap (17/19). Stemohyoideus flap (1/19) and sternocleidomastoid flap (1/19). RESULTS: Three and five year-survival rates were 91.7% (11/12) and 3/5, respectively. Over-all decannulation rate was 84.2% (16/19), but 94.1% (16/17) in patients with rotary door myocutaneous flap and 0% (0/2) in both patients with sternohyoideus flap and sternocleidomastoid flap. There were 3 decannulation failures (15.8%). All patients resumed acceptable voice, 94.7% (18/19) enjoyed satisfactory phonation, but 5.3% (1/19) showed severe hoarseness. All except 2 patients returned normal swallow function. The latter 2 patients experienced mild abnormal swallow during eating fluid food in early stage of surgery, but had normal swallow function after 1-2 weeks. CONCLUSION: Functional laryngectomy is a radical operation in selected cases with advanced laryngeal cancer. The second stage reconstruction with bi-pedical rotary door myocutaneous flaps can help decannulation in patients who developed laryngostenosis after partial laryngectomy and extended partial laryngectomy, and restore the essential function of larynx and normal neck appearance.

Adult↗