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Biomedical subjects

H Ueda

Publications and source records attributed to H Ueda.

At least 901 records · Page 50Linked to original sources
PubMed1980

Immunoglobulins in newborns, particularly in term SFD infants.

The following results were obtained by analyzing serum IgG, IgM and IgA of blood from the umbilical cord chiefly of small-for-date infants (term SFD infants): (1) The serum IgG level was higher in the infant's blood than in the mother's blood in the cases of appropriate-for-date infants (term AFD infants). Its fetus/ mother ratio was 1.25 +/- 0.22. (2) The fetus/mother ratios of IgG in term SFD infants and premature infants were lower than in term AFD infants. (3) The placental transmission of IgG increased as the gestation weeks went by, and its fetus/mother ratio reached 1.0 by the 38th week of pregnancy. (4) The serum IgM level was lower in term SFD infants than in term AFD infants. (5) Term SFD infants showed no difference of serum IgA level from that of term AFD infants.

Female↗
PubMed1980

Retinoic acid-binding protein in a human cell (MCF-7) from breast carcinoma.

A specific retinoic acid-binding protein was demonstrated by sucrose density gradient centrifugation and saturation binding analysis in MCF-7 human breast cancer cells. In contrast, retinol-binding protein could not be detected in this cell line. By Scatchard analysis, this retinoic acid-binding protein was found to have a dissociation constant (Kd) of 154 nM and to bind a maximum of 14 pmoles of [3H] retinoic acid per milligram of cytoplasmic protein. Experiments with intact attached cells revealed the Kd to be 125 nM, which is very close to the value obtained for cytoplasmic extract. The binding of [3H] retinoic acid was abolished by unlabeled retinoic acid. Retinal and alpha-retinoic acid competed for binding sites but were less potent than unlabeled retinoic acid. Retinol, retinyl acetate, and the analog Ro 10-9359 showed little of no competition for the retinoic acid-binding site. A specific retinoic acid-binding protein was also demonstrated by gel electrophoresis. The presence of retinoic acid binding protein in MCF-7 cells suggests that the biologic effects of retinoic acid may be mediated by this specific protein.

Breast Neoplasms↗
PubMed1979

A novel analgesic dipeptide from bovine brain is a possible Met-enkephalin releaser.

It is generally accepted that morphine exerts its analgesic effect by binding to specific opiate receptors in the brain and spinal cord. Since Hughes et al. isolated and identified two endogenous pentapeptides, Met- and Leu-enkephalin, from the brain and found that they acted as agonists at opiate receptors, alpha-, beta- and gamma-endorphins, larger peptides than enkephalins and having morphine-like activity, have been identified in either the brain or pituitary of various species. Several studies have demonstrated that enkephalins possess analgesic properties and that they are distributed in the pain-mediated pathways in the central nervous system. These findings suggest that enkephalins are important neurotransmitters or neuromodulators regulating pain transmission. We now report the isolation of a novel substance which has a Met-enkephalin releasing action. Our findings suggest the possibility of a regulating mechanism for the release of endogenous opioid peptides, especially Met-enkephalin.

Analgesia↗
PubMed1979

Comparison of the analgesic effects of various opioid peptides by a newly devised intracisternal injection technique in conscious mice.

To determine accurately the central analgesic effects of opioid peptides, we devised a special technique for intracisternal administration of drugs to conscious mice. When this method was utilized we found that a series of endogenous opioid peptides and enkephalin analogues, (D-Ala2,Met5)-enkephalinamide and (D-Met2,Pro5)-enkephalinamide produced dose-related analgesic effects, as determined by the tail pinch test. These effects were antagonized by pretreatment with naloxone 0.5 mg/kg s.c. This technique should be a most accurate one for the determination of central analgesic effects of various drugs in conscious mice.

Animals↗
PubMed1979

Pain and the bulbospinal noradrenergic system: pain-induced increase in normetanephrine content in the spinal cord and its modification by morphine.

Experiments were carried out to determine whether noxious stimulation produced biochemical changes in noradrenergic neurons in the spinal cord of rats and whether morphine induced different biochemical effects in the presence and the absence of noxious stimuli. Noxious stimuli, but not stress, significantly increased the normetanephrine (NM) concentration only in the dorsal half of the spinal cord without affecting the noradrenaline concentration. The NM increase induced by noxious stimuli remained after transection at the inter-collicular level. The NM increase induced by noxious stimuli remained after transection at the inter-collicular level. When the noxious stimulation was blocked by lidocaine at the peripheral level, the NM increase disappeared. THe NM concentration elevated by pain, was further increased by morphine. The data suggest that the bulbospinal noradrenergic system works as a pain control system: pain itself slightly activates this system, but not to the extent of producing analgesia and the addition of morphine further activates this system to a level sufficient to produce analgesia.

Animals↗
PubMed1979

Use of curves for prediction of maternal blood levels of placenta-specific substances for diagnosis of placental function.

Maternal blood levels of cystine aminopeptidase (CAP), human placental lactogen (HPL) and beta 1 glycoprotein (SP-1) were predicted and evaluated using the expressions and their charts developed by us to help diagnosis of placental function in women of the third trimester of pregnancy. This study was conducted on the assumption that these placenta-originating substances as markers would behave similarly to the previously reported heat-stable alkaline phosphatase (HSAP). The results realized the following features: (1) CAP, HPL and SP-1, like HSAP, had their normal ranges of values too wide to be based on for diagnosing placental function in general, but it was confirmed that on the individual basis these marker substances could develop adequate "prediction curves" for their values to come well answering to the test in the same way as with HSAP. (2) The expressions for predicted values revealed that these marker substances in their shift in the maternal blood had different critical points start of deviation from exponential rising. Particularly in abnormal pregnancy, their shifting patterns were often dissimilar to one another, with implications that impaired placental function could possibly be confirmed qualitatively by reference to the predicted curve for the values of either of the marker substances.

Aminopeptidases↗
PubMed1979

Nonspecific adjuvant immunotherapy of lung cancer with cell wall skeleton of Mycobacterium bovis Bacillus Calmette-Guérin.

Nonspecific adjuvant immunotherapy with Bacillus Calmette-Guérin cell wall skeleton (BCG-CWS) was given to 155 lung cancer patients. Clinical effects of the BCG-CWS treatment were estimated by comparing the survival of the BCG-CWS group with that of a historical control group on the basis of 4-year results. Significant prolongation of survival time has been observed in Clinical Stages II, III (M0) and III (M1). However, most Stage III patients who were given the BCG-CWS treatment died of cancer itself after marked prolongation of survival time. An increase in complete cure rate has been expected only in Stages I and II. Surgicopathological staging was used in resected cases. Resected cases at any stage were sensitive to treatment with BCG-CWS. Histologically, all types of lung cancer including squamous cell carcinoma, adenocarcinoma, and anaplastic carcinoma were sensitive to treatment with BCG-CWS. Intrapleural administration of BCG-CWS to patients with malignant pleurisy was effective in controlling the pleural effusion and prolonging the survival time. No serious complication has been experienced in our study.

Adenocarcinoma↗