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Biomedical subjects

H Ueda

Publications and source records attributed to H Ueda.

At least 865 records · Page 48Linked to original sources
PubMed1983

[Clinical studies on combination chemotherapy with fosfomycin and dibekacin in complicated urinary tract infection].

Clinical studies were performed on combination chemotherapy with Fosfomycin and Dibekacin. Sixteen patients with complicated urinary tract infections were treated with a combination of Fosfomycin (4 g/day, d.i.v.) and Dibekacin (200 mg/day, i.m.) for 5 days; and, 15 of them were clinically evaluated by criteria of UTI committee. The clinical effects proved excellent in 3 patients, good in 8 patients, and poor in 4 patients overall effective rate was 73.3%. Out of 19 strains isolated from the patients, 12 strains disappeared after the therapy. No side effect was observed in 16 cases. Clinical use of the combination chemotherapy with Fosfomycin and Dibekacin was thought to be effective and safe for patients with complicated urinary tract infections, because the combination acts not only synergistically, but also because Fosfomycin acts to protect against the nephrotoxicity induced by Dibekacin.

Adult↗
PubMed1982

Analgesic dipeptide, kyotorphin (Tyr-Arg), is highly concentrated in the synaptosomal fraction of the rat brain.

To examine the physiological role of the analgesic dipeptide, kyotorphin (Tyr-Arg), which was isolated from the mammalian brain, its subcellular localization was studied. Kyotorphin was determined using high-performance liquid chromatography (HPLC) with an electrochemical detector. This dipeptide was found to be concentrated in the crude mitochondrial (P2) fraction. Further subfractionation of the P2 fraction revealed that kyotorphin was exclusively localized in the synaptosomal fraction.This finding suggests the possibility that kyotorphin has a neurotransmitter/neuromodulator role in the brain.

Animals↗
PubMed1982

Retinoic acid binding protein in normal and neopolastic rat prostate.

Sucrose density gradient analysis of cytosol from normal and neoplastic rat prostatic tissues exhibited a peak of (3H) retinoic acid binding in the 2S region, corresponding to the cytoplasmic retinoic acid binding protein (cRABP). In the Fisher-Copenhagen F1 rat, cRABP was present in the lateral lobe, but could not be detected in the ventral nor in the dorsal prostatic lobes. Four sublines of the R-3327 rat prostatic tumor contained similar levels of this binding protein. The absence of cRABP in the normal tissue of origin of the R-3327 tumor, the rat dorsal prostate, and reappearance in the neoplastic tissues follows a pattern described in other human and animal tumors. The occurrence of cRABP in the well-differentiated as well as in the anaplastic R-3327 tumors in which markers which reflect a state of differentiation and hormonal regulation, such as androgen receptor, 5 alpha reductase, and secretory acid phosphatase are either markedly reduced or absent, points to cRABP as a marker of malignant transformation.

Adenocarcinoma↗
PubMed1982

X-ray microanalysis of hydrated biological specimens.

Using a wide angle backscattered electron detector (BED), glutaraldehyde fixed or unfixed specimens of biological soft tissues such as hen oviduct, kidney, liver, duodenum of mouse as well as mitochondrial fraction from rat liver were observed under low vacuum (0.3 to 0.5 torr) at magnifications from x300 to 10,000. The backscattered electron images (BEI) of glutaraldehyde fixed hen oviduct were correlated to X-ray microanalysis. Intracellular secretion granules in such unhydrated, uncoated and unstained slices of hen oviduct preserved Ca even after glutaraldehyde fixation. Backscattered electron images of unfixed duodenum and kidney of mice were not satisfactory for observing intracellular structures, though X-ray microanalysis could detect P,S,Cl and K over cut surfaces of such hydrated unfixed tissue. Compared with frozen dehydrated specimen, the hydrated unfixed tissue tends to preserve more potassium and less chlorine. Cytochemical reaction product of succinate dehydrogenase (SDH-ase) activity in isolated mitochondria from rat liver was detectable in hydrated condition. Quick and easy assessment of localized elements in surgical specimens by combination of wet-SEM and X-ray microanalysis will be a new tool for clinical application of SEM. In spite of obvious limitations of this method, especially of its spatial resolution both in backscattered electron image and in bulk specimen X-ray microanalysis, the combination of wet-SEM and X-ray microanalysis provides information which has not been available in the past.

Animals↗
PubMed1981

Mechanism of kyotorphin-induced release of Met-enkephalin from guinea pig striatum and spinal cord.

The characteristics of kyotorphin (Tyr-Arg)-induced release of Met-enkephalin from the striatum and the spinal cord of guinea pig were determined by superfusing the slices in vitro and then carrying out radioimmunoassays. Depolarization by 50 mM K+ induced a marked release of Met-enkephalin-like immunoreactivity. The potassium-induced release of Met-enkephalin was calcium-dependent. In preparations from a striatum, the addition of kyotorphin to the superfusion medium produced a concentration-dependent increase in Met-enkephalin. The kyotorphin-induced release of Met-enkephalin was calcium-dependent and was abolished by tetrodotoxin. Similar effects of kyotorphin were seen in the spinal cord preparations. Electrical field stimulation of the striatal slices at a frequency of 10 Hz also evoked significant and calcium-dependent increases in the release of Met-enkephalin and markedly enhanced the kyotorphin-induced release of Met-enkephalin, as compared to the controls not given field stimulation. These results suggest that kyotorphin depolarizes the so-called enkephalinergic neurons and releases Met-enkephalin from nerve terminals. This effect of kyotorphin may be a possible mechanism related to the manifestation of analgesia.

Animals↗
PubMed1981

Excretion of indomethacin into saliva following intravenous administration to dogs.

Parotid saliva (Pr) and mandibular-sublingual saliva (MS) were collected separately by means of permanent fistulae in order to investigate the excretion of indomethacin in saliva of dogs receiving a single intravenous dose of 20 mg/kg. Drops of citric acid solution were placed on the tongue to stimulate salivary secretion. The concentrations of indomethacin both in saliva and plasma declined biexponentially with time. There was a good linear relationship between the drug concentration in each saliva and plasma. The Pr and MS levels were 7.4% and 4.4% of the plasma levels, respectively. Indomethacin concentrations in Pr were significantly higher than in MS (p less than 0.05). The roles of salivary pH and salivary protein binding were discussed in respect to the mechanism of salivary excretion of the drug.

Animals↗