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Biomedical subjects

H Shoji

Publications and source records attributed to H Shoji.

At least 181 records · Page 10Linked to original sources
PubMed1984

Identification of fibronectin in preparations of osteoarthritic human cartilage.

Several high molecular weight proteins were observed in dissociative extracts of osteoarthritic, but not of normal, human cartilage. By gel electrophoresis, by DEAE-cellulose and gelatin-agarose chromatography, and immunologically, they were found to be identical to fibronectin. Incorporation of tritiated proline into these proteins indicated that this material was not a synovial fluid contaminant. Interactions with the proteoglycans suggested that, in articular cartilage, the role of fibronectin may be more closely associated with proteoglycans than with collagen. The appearance of fibronectin in the diseased cartilage suggests that this may be a feature of the chondrocyte's repair response to the loss of extracellular matrix.

Cartilage, Articular↗
PubMed1984

Type of collagen in chordoma.

Pepsin-soluble collagen was extracted from three histologically proven cases of chordoma and nucleus pulposus. The collagen types of these materials were investigated by differential salting-out, SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) of native collagen and their CNBr (cyanogen bromide) cleaved peptides, and their amino acid compositions. Although the collagen of nucleus pulposus was type II, that of chordoma was largely type I. Collagen of notochord, the origin of nucleus pulposus, is known to be type II. Further investigation is necessary in view of the fact that collagen of chordoma, a tumor believed to be derived from notochord, is not type II.

Aged↗
PubMed1983

A search for rheumatoid arthritis-associated nuclear antigen and Epstein-Barr virus specific antigens or genomes in tissues and cells from patients with rheumatoid arthritis.

Pannus extracts and/or cells and tissues from rheumatoid arthritis (RA) patients and controls were tested for the presence of RA-associated nuclear antigen (RANA), anti-RANA, Epstein-Barr virus (EBV)-specific antigens, and EBV genomes. Using our present rather insensitive methods, RANA was rarely detected in RA pannus extracts or tissues, whereas anti-RANA was commonly found. EBV-specific antigens or genomes could not be detected in a limited number of tissues tested using our present methods. The data suggest that EBV may not be the primary event causing RA through direct infection and transformation of synovial tissues.

Antibodies, Antinuclear↗
PubMed1983

Biochemical features of pseudomembrane at the bone-cement interface of loosened total hip prostheses.

Biochemical features of pseudomembrane formed at the bone-cement interface of 13 failed total hip replacements were studied and histological examination of the pseudomembrane was conducted. The results of biochemical analyses revealed on average hydroxyproline, 86 micrograms/mg; water content, 57%; hexosamine, 12 micrograms/mg; glucosamine/galactosamine, 1.4%; and calcium, 2.1%. Approximately 78% of collagen was extracted with pepsin, whereas only 8 and 0.3% collagen were extracted with acetic acid and neutral salt, respectively. On further differential salting out of the extracted collagen, about 77% was type I collagen, 19% type III, and 2.6% type V. Unlike pseudoarthrosis of fracture, no type II collagen was identified. Overall biochemical features in the light of the histological appearance suggested that pseudomembrane resembles granulation tissue. Bone healing mechanisms at the bone-cement interface appear to differ from that of fracture healing.

Adult↗
PubMed1983

Voice disorders without organic diseases of the larynx. A 10-year review of 62 patients.

We reviewed the clinical records of 62 patients with voice disorders without organic diseases of the larynx who were examined in the Department of Otolaryngology, Kurume University Hospital during the 10 years from 1971 to 1980. There were 9 patients with psychogenic dysphonia, 24 with vocal abuse, 4 with spastic dysphonia, 9 with mutational voice disturbance, 8 with virilization of voice, 7 with dysphonia attributed to diseases of other organs than the larynx, and 1 with senile change of voice. The clinical service to the patients with these kinds of dysphonia has been poor in Japan for lack of trained voice pathologists. This has been also the case with our department. Recognition for the necessity of voice pathologists is strongly demanded.

Adolescent↗
PubMed1982

Compositional changes of glycosaminoglycans of the human menisci with age and degenerative joint disease.

The compositional profiles of meniscal glycosaminoglycans (GAG) at different ages and in the presence of osteoarthritis were determined. The major components of meniscal GAG were chondroitin sulphates and dermatan sulphate. Other major elements were keratan sulphate and hyaluronic acid. Chondroitin sulphate-4 and -6 decreased with age. Dermatan sulphate content remained unchanged with age whereas keratan sulphate and hyaluronic acid increased. GAG compositional profiles of meniscus from osteoarthritis were similar to those of comparable age group. The results indicate that GAG compositional make-up of the meniscus changes with age. These changes may make it more susceptible to horizontal tears in the elderly.

Adolescent↗