With the reflectance meter Reflolux S (Accucheck III) high as well as low blood glucose levels can be determined with excellent precision irrespective of the sample's hematocrit value. Within the range from 20-50 mg/dl we found an average deviation from the expected value of less than 2 mg/dl; in selected samples, however, a difference of up to 10 mg/dl was recorded. The scatter can be explained by the influence of hematocrit values on the results. An increase of 10% in hematocrit lowered the measured glucose value by approximately 10%. At glucose concentrations below 40 mg/dl and in the presence of high resp. low hematocrit values any results obtained with this glucose meter will only have screening character; they should be re-run using an established method.

Blood Chemical Analysis↗

PubMedDate not supplied

[Reference values for the concentration of free triiodothyronine (FT3), triiodothyronine (TT3), free thyroxine (FT4), thyroxine (TT4), thyrotropin (TSH) and thyroxine-binding globulin (TBG) in umbilical cord blood. Method: luminescence-enhanced enzyme immunoassay].

Physiological concentrations of FT3, TT3, FT4, TT4, TSH and TBG were determined using the luminescence enhanced enzyme immunoassay method in cord blood serum (n = 100). The results are presented in Table 1.

Fetal Blood↗

PubMedDate not supplied

[Comparison of free amino acids in capillary and venous blood serum of children and adolescents with phenylketonuria and leucinosis].

Concentrations of free amino acids were determined quantitatively by ion exchange column chromatography in serum from capillary and venous blood of children and adolescents with phenylketonuria (n = 38) and leucinosis (n = 3). Serum was deproteinised by 5% sulphosalicylic acid (1:1, v/v). The results showed with exception of aspartic acid, glutamine and glutamic acid a very close correlation. The correlation coefficients ranged from 0.849 to 0.996. It is concluded that serum from capillary blood can be used for screening of amino acid metabolism.

Adolescent↗

PubMedDate not supplied

[Comparison of the ferritin and iron concentration in capillary and venous blood serum of children, adolescents and adults].

The ferritin and iron concentration were compared in sera from capillary and venous blood (n = 52). Ferritin was determined using a luminescence-enhanced enzyme immunoassay and iron by the ferrozine method. The results showed a very good correlation and a linear relationship for ferritin (r = 0.999) but not for iron (r = 0.855). It is concluded that capillary blood serum can be used only for the determination of ferritin.

Adolescent↗

PubMedDate not supplied

[Reference values for blood levels of free thyroxine, free triiodothyronine and thyroxine-binding globulins in euthyroid children. Method: luminescence-enhanced enzyme immunoassay].

Concentrations of free thyroxine (n = 296, male 164, female = 132), free triiodothyronine (n = 273, male 156, female = 117) and thyroxine-binding globulin (n = 273, male 156, female = 117) were determined using the luminescence enhanced enzyme immunoassay method in blood-serum of euthyroid children. Beyond the 30, day of life no significant differences were found in different age groups for the free thyroxine and beyond the 2, day of life for the free triiodothyronine concentration. The free thyroxine values of the infants in the range of 15-39 pg/ml decreased to the range of 9-18.5 pg/ml and the free triiodothyronine values decreased from 3.4-9.3 pg/ml to the range of 2.8-6.5 pg/ml. The values for thyroxine-binding globulin increased in the first month of life up to the range of 18-35 mg/l and decreased afterwards continuously to the range of 14.5-26.5 mg/l.

Adolescent↗

PubMedDate not supplied

[Serum fructosamine in healthy and diabetic children and adolescents].

The determination of fructosamine in serum of healthy newborns, children and adolescents by a new colorimetric method leads to an age-dependent reference range. Correction of the values for total protein yields a median of the results, which is not dependent on age and the upper limit of the reference range is not significantly different from that of healthy adults; this does not hold true for referral to albumin. As the half-life time of glycated serum proteins is shorter (mean 20 days) than that of hemoglobin A1c, fructosamine can provide useful additional informations about the diabetic control of children and adolescents (medium-term record of blood glucose).

Adolescent↗