Biomedical subjects

H Schlebusch

Publications and source records attributed to H Schlebusch.

98 records · Page 6Linked to original sources

PubMedDate not supplied

[Reference values of the ferritin concentration in the serum in children].

Concentrations of ferritin were determined using the luminescence enhanced enzyme immunoassay method in serum of 272 (male = 148, female = 124) "healthy" children. The ferritin concentration in children is age dependent. The newborn values (140.1-673.8 micrograms/l, 95% confidence interval) decrease until the end of the first year and stabilize thereafter in a sex specific fashion: male means = 31.90 micrograms/l (95% confidence interval: 14.0-72.2 micrograms/l), female means = 39.23 micrograms/l (95% confidence interval: 16.1-79.9 micrograms/l).

Adolescent↗

PubMedDate not supplied

[Reference values of fasting serum levels of free amino acids in children].

Concentrations of free amino acids were determined quantitatively by ion exchange column chromatography in serum from the fasting blood of 352 (male = 177, female = 175) "healthy" infants, children and adolescents. Serum was deproteinised by 5% sulphosalicylic acid (1:1, v/v). The results -95% confidence range-are presented age-dependent (groups: up to 1 month, 1-24 months, 2-6 years, 6-10 years, 10-14 years) and sex-dependent in table 4 and 5.

Adolescent↗

PubMedDate not supplied

[Age-specific and sex-specific reference values for the excretion of free amino acids in the 24-hour urine of children].

Free amino acids were determined quantitatively by ion exchange column chromatography in 24 h urine of 354 (male = 187, female = 167) "healthy" infants, children and adolescents. Urine was deproteinised by 5% sulphosalicylic acid (1:1, v/v). The results - 95% confidence range - are presented age-dependent (groups: up to 1 month, 1-24 months, 2-6 years, 6-10 years, 10-14 years) and sex-dependent in table 3 and 4.

Adolescent↗

PubMedDate not supplied

[Determination of bilirubin in capillary plasma by a direct photometric method (DPM, bilirubinometer) and the chemical determination of bilirubin in the bilirubin determination (2,5-dichlorophenyldiazonium method) in serum of venous blood samples].

The determination of bilirubin in serum was performed by the 2.5-dichlorphenyldiazonium method (DPD) and in capillary plasma by the direct photometric method (DPM). Both methods showed a good precision and accuracy. The investigation was carried out in 135 samples with a bilirubin concentration up to 25 mg/dl. The comparison of the two methods in 62 samples with a bilirubin concentration up to 10 mg/dl showed a correlation coefficient of r = 0.862 and in 73 samples with a bilirubin concentration between 10 and 25 mg/dl a correlation coefficient of r = 0.893. In 29 cases (21.5%) we found differences between the two methods of 1.5-4.0 mg/dl. Most of them were in the critical higher range. Discussion of the DPD and DPM methods.

Bilirubin↗

PubMedDate not supplied

[Reference values for the concentrations of triiodothyronine, thyroxine and thyrotropin in the blood serum of euthyroid children. Method: luminescence-enhanced enzyme immunoassay].

Concentrations of triiodothyronine, thyroxine and thyrotropin were determined using the luminescence enhanced enzyme immunoassay method in blood serum of 343 (male = 197, female = 146) euthyroid children. Beyond the newborn age no significant difference was found for the thyrotropin concentration. Compilation of the values resulted in a physiological thyrotropin concentration of 1.73 +/- 0.81 (1s) microU/ml for infants, children and juveniles. In opposition to these results the thyroxine and triiodothyronine concentration showed an age-specific dependence. The thyroxine and triiodothyronine concentrations (means +/- 1s) decreased continuously from the infant age (TT4: 182.1 +/- 37.7 ng/ml, 234.9 +/- 48.6 nmol/l, TT3: 1.77 +/- 0.57 ng/ml, 2.73 +/- 0.88 nmol/l) to the juvenile age (TT4: 77.9 +/- 14.3 ng/ml, 100.5 +/- 18.4 nmol/l, TT3: 1.25 +/- 0.31 ng/ml, 1.93 +/- 0.48 nmol/l). The luminescence enhanced enzyme immunoassay technique is suitable for use in the routine thyroid laboratory.

Adolescent↗

PubMedDate not supplied

[Comparison of the luminescence enhanced enzyme immunoassay Amerlite FT4/MAB-FT4 and Amerlite FT3/MAB-FT3 in blood serum of children. Reference values].

Concentrations of free thyroxine (n = 225, male = 124, female = 101) and free triiodothyronine (n = 60, male = 35, female = 25) were determined using the luminescence enhanced enzyme immunoassay methods Amerlite FT4/MAB-FT4 and Amerlite FT3/MAB-FT3 in blood-serum of children. In contrast to the excellent agreement of the results between the Amerlite FT4 and MAB-FT4 method, a significant difference was found between the Amerlite FT3 and MAB-FT3 results. Therefore it was necessary to establish new reference ranges for the MAB-FT3 method in childhood (cord blood = 50, n = 272, male = 183, female = 89).

Adolescent↗

PubMedDate not supplied

Influence of NH(4)CI on polarized release of endogenous protein degradation products and on morphology in LLC-PK(1) cells.

Increased renal ammoniagenesis is thought to be a causative factor for renal hypertrophy which occurs in several disorders accompanied by metabolic acidosis. We studied the influence of ammonia on the polarized release of degradation products of endogenous proteins in LLC-PK(1) cells. Release of acid-soluble radioactivity to the extracellular space decreased under the influence of NH(4)Cl with a remarkable transient reduction of basolateral release. Electron microscopically NH(4)Cl-treated cells showed numerous enlarged lysosomes suggesting an accumulation of incompletely degraded cytoplasmatic material in the lysosomal compartment. We conclude that split products of lysosomal degradation of endogenous proteins are preferentially transported to the extracellular space via the basolateral plasma membrane.

Ammonium Chloride↗

PubMedDate not supplied

Blood glucose determinations in newborns: four instruments compared.

Four portable analyzers, HemoCue B-Glucose (I), Accu-Check III (II), One-touch II (III), and Glucometer Elite (IV), with different measuring principles were tested for their suitability for measuring blood glucose in neonates. Precision of all instruments is satisfactory. In the analysis of capillary blood from newborns, two instruments show an excellent accuracy; however, the scatter of the results for instrument (II) is about 1.6 times greater than for instrument (I). The inaccuracy of instruments (III) and (IV) is not acceptable from a clinical point of view. All devices show an influence of hematocrit, the magnitude of which varies between 5% (I) and 12% (III) for every 10% change of hematocrit. Instruments II and IV show that temperature has a marked influence on the readings; the same is true for oxygen in instrument IV. In conclusion, only instrument (I) has met the requirements of accurate and precise blood glucose determinations in neonates.

Blood Chemical Analysis↗