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Biomedical subjects

H Sakaguchi

Publications and source records attributed to H Sakaguchi.

At least 397 records · Page 22Linked to original sources
PubMed1979

Finger print deposits of the kidney in pure monoclonal IgG kappa cryoglobulinemia.

In a male patient with pure monoclonal IgG kappa cryoglobulin associated with the nephrotic syndrome, finger print deposits were observed in the subendothelial space and mesangial matrix of the kidney by electron microscopy. The structure was similar to a unique tubular crystal structure of IgGl kappa cryoglobulin which was reported by Bogaars et al. [1973] in the serum of a patient with multiple myeloma. It was therefore supposed that the finger print deposits in the kidney of this patient were the precipitation of the monoclonal IgG kappa cryoglobulin in serum.

Cryoglobulins↗
PubMed1978

GI absorption of beta-lactam antibiotics I: kinetic assessment of competing absorption and degradation in GI tract.

An equation was derived for the simultaneous assessment of rate constatns for absorption and nonenzymatic degradation of unstable drugs in in situ absorption experiments. The equation was substantiated by using a variety of beta-lactam antibiotics in the recirculation technique through the rat small intestine. Plots of the apparent first-order rate constant for the disappearance of the drug from the gut lumen versus the reciprocal of the volume of recirculating solution yielded a straight line with a slope equal to the intrinsic absorption rate constant and with an intercept equal to the nonenzymatic degradation rate constant in the GI lumen. The kinetic method for evaluation of the absorption rate constant also was developed for a more complex situation in the GI lumen involving absorption, nonenzymatic degradation, and enzymatic metabolism. The proposed method was confirmed with carbenicillin indanyl, which was metabolized rapidly to carbenicillin by the action of nonspecific esterase in the intestine. In the absence of information of Michaelis--Menten kinetic parameters, the present method is advantageous for evaluation of the intrinsic absorption rate of all unstable drugs.

Animals↗
PubMed1978

Studies on the formative mechanism of mesangiolytic changes of renal glomerulus induced by a low calcium diet breeding.

Mucopolysaccharides (MPS) are widely distributed in the connective tissues throughout the animal body. There are a lot of MPS classified both chemically and immunochemically and solutions of these various polysaccharides are characterized physicochemically by their high viscosity. In this study guinea pigs were bred on a low calcium diet of 0.2% for 13 weeks and the mesangiolytic changes of renal glomerulus--the partial cystic dilatation of the glomerular tufts with edematous loosening of the mesangial tissue were shown light microscopically. The less dense mesangial matrix with prominence of mesangial fibrils and small cytoplasmic projections of the mesangial cells were demonstrated electron microscopically. The formative mechanism of these mesangiolytic changes of renal glomerulus induced by a low calcium diet breeding will be discussed in this paper.

Animals↗
PubMed1978

Mechanisms of immune deposition in relation to a loosened molecular structure of acid mucopolysaccharides (aMP) caused by a low calcium diet--with special regard to their negative charges.

Diets used for breeding guinea pigs usually require 1.3--1.5% calcium. In the previous paper, guinea pigs bred on a low calcium diet of 0.2% for 4--6 weeks, showed mesangiolytic changes in the glomerulus. In this experiment, after breeding guinea pigs on a low calcium diet, soluble immune complexes which have positive charges in their structure, were injected once intravenously. By electron microscopy an increased amount of deposits in the basement membrane, in the subepithelial side of it, and in the mesangial matrix where they were loosened by a low calcium diet breeding, as reported in the previous paper, was noted. In this paper, mechanisms of immune deposition in relation to a loosened molecular structure of acid mucopolysaccharides, caused by a low calcium diet--especially their negative charges--will be discussed.

Animals↗
PubMed1978

Nephritogenic glycoprotein. VIII. Demonstration of the limited role of immune complex mechanism for the production of membranous glomerulonephritis.

Proliferative glomerulonephritis was induced in rats by a single injection in the hind footpads of the glycoprotein isolated from pronase digests of homologous renal cortex, in spite of the evidence that the glycoprotein no longer possessed the antigenic activity with respect to producing the nephrotoxic antibody. In rats given the glycoprotein (without trichloroacetic acid (TCA) treatment) prepared from pronase digests of homologous renal cortex, morphologic changes of proliferative glomerulonephritis were followed (3--4 months after injection) by those of typical membranous glomerulonephritis with an immunofluorescent 'granular' pattern, but in animals which received the glycoprotein (with TCA treatment), the morphologic changes of the kidney were those of proliferative glomerulonephritis with an immunofluorescent 'mesangial' pattern throughout the whole course. Morphologic changes of membranous glomerulonephritis induced in the group given the glycoprotein (without TCA treatment) can be explained by an immune-complex mechanism, superimposed on the basic pathogenesis (common to both groups) of proliferative glomerulonephritis with immunofluorescent 'mesangial' pattern.

Animals↗
PubMed1977

The pathogenesis of Bartter's syndrome. Functional and histologic studies.

We describe a patient with Bartter's snyndrome. In addition to the well-known characteristic findings by light microscopy, electron micrograms confirmed the presence of juxtaglomerular cell hyperplasia with polymorphous renin secretory granules and dense multivesicular bodies. Volume expansion by albumin infusion decreased plasma renin activity and aldosterone excretion, and improved the pressor response to exogenous angiotensin, suggesting that the renin-angiotensin-aldosterone system was not autonomous but that a decreased extracellular volume might be a major defect in this patient. During hypotonic saline diuresis, moreover, fractional free water clearance per fractional distal sodium delivery, CH2O/CH2O + CNa, was markedly depressed in the patient when compared with the value in the controls. Evidence presented suggests that chronic extracellular volume depletion exists as a consequence of an impaired sodium transport in the ascending limb of Henle's loop.

Adult↗
PubMed1977

The renal lesion associated with total lipodystrophy.

This report concerns a 41-year-old man with total lipodystrophy associated with a specific renal disorder. He had massive proteinuria, mild azotemia and a normal level of serum complement in addition to the generalized loss of subcutaneous fatty tissue. Results of 1 50-gm glucose tolerance test indicated a high fasting insulin level with an exaggerated response to glucose. Hypoglycemic responses to exogenous insulin were reduced. Renal biopsy revealed changes consistent with glomerular lipidosis. This case is unique in that the renal lesion was not the Kimmelstiel-Wilson pathologic change atributable to abnormal glucose metabolism.

Adult↗