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Biomedical subjects

H Meyer

Publications and source records attributed to H Meyer.

At least 325 records · Page 18Linked to original sources
PubMed1990

[The dynamics of the microbial count in experimentally-induced salmonellosis of calves].

Numerical developments of pathogens were followed up in intestinal matter, mucosa, and lymph nodes as well as in spleen and liver of calves which had been orally infected with Salmonella (S.) dublin or S. typhimurium. Massive flooding of the organism with Salmonellae and eventually fatal proliferation were found to be possible immediately after infection or after some days of pathogen-host equilibrium. Complete elimination of Salmonellae was found to be achievable even after infestation of spleen and liver. Intestinal lymph nodes, in addition to intestinal mucosa and intestinal matter, proved to be of decisive relevance to pathogenesis of the infection and to persistence of germs.

Animals↗
PubMed1990

[Meadow saffron (Colchicum autumnale) in hay and colic in horses].

Following feeding of a new delivery of hay (bought in Southern Germany) 3 horses out of a unit of 17 developed colic within a few days one horse died. Post-mortem there was an intensive accumulation of serous or serous-haemorrhagic fluid in the thorax and abdomen. The hay was heavily contaminated by autumn crocus (colchicum autumnale)--about 1.48% of total mass. Other feedstuffs were of good quality and following removal of the affected hay no further cases of colic occurred. The pathological findings also indicated a colchicine intoxication.

Animal Feed↗
PubMed1989

[Changes in fatty acid metabolism in dilated cardiomyopathy and hypertrophic cardiomyopathy in childhood].

The aetiology and the pathomechanisms in both types of cardiomyopathy (CM) are still unknown. In vivo measurements of myocardial metabolism in CM may be useful, but there is hardly any information on this subject. Free fatty acids (FFA) are the main source of energy for the normal myocardium. A method for external measurement of the FFA extraction rate (FFA-ER) in different myocardial regions by the simultaneous use of two isotopes has been developed. 201 TI indicates the myocardial perfusion and 15-(p-123 I-phenyl)-pentadecanoid acid (IPPA) represents the FFA uptake. The relation of IPPA/TI reflects the FFA-ER. 8 patients with hypertrophic CM (HCM), age 0.2-20 years, and 8 patients with dilated CM (DCM), age 0.2-18 years were investigated. 12 healthy adults and 4 infants after an arterial switch operation were used as a control group. All patients with HCM showed normal myocardial perfusion but the FFA uptake was strongly diminished, resulting in a reduction in FFA-ER to 42 +/- 12% of the normal value. The maximal influx rate (IR) of FFA was diminished too. In patients with DCM both the myocardial perfusion and the FFA uptake were globally reduced, resulting in a virtually normal FFA-ER. The IR was slightly increased. In HCM and DCM FFA utilisation was disturbed. The alterations were significantly different in both types of CM and different pathomechanisms can be assumed.

Adolescent↗
PubMed1989

Traumatic shock and head injury: effects of fluid resuscitation on the brain.

UNLABELLED: The effects of resuscitation of traumatic-hemorrhagic shock on the brain are unknown. Traumatic shock in sheep (fracture/crush injury, 2-hr hemorrhage to 40 mm Hg) was followed by resuscitation to baseline mean arterial pressure. Two groups without brain injury were resuscitated with lactated Ringer's (LR1, n = 7) or albumin (ALB1, n = 6). Focal brain injury was added in two further groups (LR2, n = 6; ALB2, n = 6). Hemodynamics, intracranial pressure (ICP), EEG, and colloid osmotic pressure (COP) were followed. Brain water (BW) and cerebral blood volume (CBV) were compared to those of controls (C, n = 7). RESULTS: ICP rose in all groups. Animals without brain injury did not have increased brain water. Below are results for brain-injured animals after resuscitation (mean +/- SEM). (table; see text) Maintaining COP during initial resuscitation does not minimize cerebral edema: the effects of LR and ALB were similar in this setting. Focal brain injury causes edema but does not cause large increases in ICP with initial resuscitation.

Albumins↗
PubMed1989

Establishment of an ELISA for the detection of orthopox viruses based on neutralizing monoclonal and polyclonal antibodies.

Various combinations of polyclonal and neutralizing monoclonal antibodies (MAbs) of distinct specificity were evaluated as capture or detecting antibodies in an orthopox virus antigen ELISA. Acceptable results were achieved in an assay based on polyclonal antibodies. A 10 times higher sensitivity, however, was obtained using a combination of one monoclonal catching antibody reactive with viral envelope epitopes and polyclonal detection antibodies. This configuration proved to be superior in sensitivity to all others. Specificity was confirmed with 5 vaccinia virus strains (including one recombinant virus) and 8 species of orthopox viruses. Monkeypox and mousepox virus reacted exclusively in the polyclonal assay due to a lack of the specific epitope for the monoclonal antibody. The detection limit compared to the infectivity titer amounted to 10(3)-10(4) in the monoclonal/polyclonal and to 10(4)-10(5) TCID50/0.1 ml in the polyclonal combination. The correlation between infectivity- and ELISA-titer was demonstrated by a study of the replication cycle of the rabbitpox virus Uetrecht in the permanent rabbit kidney cell line RK-13. With the established ELISAs vaccinia virus could also be detected in organ suspensions of lethally infected NMRI-mice, depending on the level of infectivity.

Animals↗
PubMed1989

Identification and characterization of a human cytomegalovirus gene coding for a membrane protein that is conserved among human herpesviruses.

A rabbit antiserum was raised against envelope material from purified human cytomegalovirus strain AD169. The serum recognized polypeptides 200, 170, 160, 75, 58, and 45 kilodaltons in size. It was used to screen a cDNA library constructed from poly(A)+ RNA from human cytomegalovirus-infected cells in the expression vector lambda gt11. A recombinant bacteriophage expressing cytomegalovirus-specific sequences was identified, and the corresponding gene was mapped to the HindIII R fragment. The gene is transcribed into a late 1.5-kilobase RNA. The nucleotide sequence of the coding region was determined. Computer analysis of the gene product revealed a polypeptide containing multiple potential membrane-spanning domains, representing a type of protein not identified in the envelope of herpesviruses before. The protein shows homology on the amino acid level to hypothetical proteins from reading frames BBRF3 of Epstein-Barr virus, UL10 of herpes simplex virus type 1, and ORF50 of varicella-zoster virus. By using an antiserum raised against procaryote-expressed parts of the cytomegalovirus membrane protein, a 45-kilodalton structural component of the virus was identified as the gene product.

Amino Acid Sequence↗
PubMed1989

[Experimental detection of the paraspecific effects of purified and inactivated poxviruses].

The paraspecific effect of pox viruses i.e. stimulation of the nonspecific part of the complex immune system, was demonstrated in vitro and in vivo for 3 different types of virus (vaccinia virus, avipox virus, parapox virus). By means of purification, concentration and inactivation, it was possible to prove that the paraspecific effect is brought about by the pox virus particle per se. The effect is induced by certain epitopes in the complex structure of the virus and is independent of virus multiplication. Both purified pox viruses and inactivated, purified pox viruses stimulate NK cell activity, the CSA and the formation of interferon, and reach higher efficacy indices in a challenge test with baby mice using stomatitis vesicularis virus than non-purified and non-inactivated cell culture material. The degree of purity of the pox virus preparations was established using the electron microscope and monoclonal antibodies. The purified and concentrated pox virus preparations proved to be pyrogen-free; they were not toxic to baby mice in vivo. An endotoxin-induced effect could be ruled out. The broad paraspecific effect of pox viruses and their structural components lends itself to use as paramunity inducers in prophylaxis and therapy.

Animals↗
PubMed1989

[The prevention and control of salmonellosis in cattle and swine].

The control of salmonellosis in the national livestock of the GDR is carried through on the basis of central regulations issued by the Head of the Veterinary Service. The crucial points of these programmes are zoo-sanitary, organizational and immunoprophylactic measures. The effectiveness of the immunoprophylaxis against salmonellosis in cattle and pigs has increased after using live vaccines. At present, the elimination of the host-adapted serovars and the decrease of serovars important for infections in man is focused on. Particular attention is payed to the serovars S. typhimurium and S. enteritidis. The objective requires a well coordinated cooperation between the Veterinary Service and the Public Health Service.

Animals↗
PubMed1989

[Overdenture screws].

The progressive significant characteristics of the cover-denture-screw are proved. The computer-recordings of the cover-denture-screw don't show any extreme maxima at a complex distribution of tension because of the favourable form-design of the screw. An obvious low concentration of tension mainly in the cervical part of the screw is the result of the special form of the implant.

Dental Implantation, Endosseous↗