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Biomedical subjects

H Klug

Publications and source records attributed to H Klug.

At least 37 records · Page 2Linked to original sources
PubMed1983

[Electron microscopy study of insufficient great saphenous veins].

Results of electron microscopic investigations of the vena saphena magna in varicosis are reported. The proximal and distal portion were used in each case. There were neither unambiguous ultrastructural differences on the endothelial cells nor a distinctly different number of Weibel-Palade bodies within the endothelial cells. Compared to this, a more massive dysplasia of the media was ascertained in the distal portion than in the proximal one. It is possible that this represents the structural equivalent of a different pathophysiological reaction which is expressed ultrastructurally in form of vacuolic cytoplasm and disintegration of myofilaments.

Cytoskeleton↗
PubMed1982

[The ultrastructure of megathrombocytes in May-Hegglin's anomaly].

A clinical and extensive electron microscopic study was made of the circulating granulocytes and thrombocytes from three female patients of a family with May-Hegglin anomaly. There have been observed inclusion bodies (Döhle-bodies) in a high proportion of polymorphonuclear leukocytes as well as a thrombocytopenia and giant platelets (so-called megathrombocytes). Other family members did not show hematologic abnormalities. The investigated giant platelets show ultrastructurally a different electron density of the alpha-granules, however, these structures present no typical formation of "bull's eye granules" which has been observed in other cases of May-Hegglin anomaly. Peripheral or central situated net-like structures of the endoplasmic reticulum have been found frequently. There were seen no microtubuli whereas glycogen particles were scattered within the peripheral cytoplasm.

Adult↗
PubMed1982

Submicroscopical aspects of the melanogenesis and malignant melanomas.

The most important pigment of the human organism is the melanin. After a short representation of the most essential reactions of the biosynthesis of eu- and pheomelanins there are discussed some aspects of the formation of melanosomes as well as exhibited the structure of eumelanosomes and pheomelanosomes. In the tumour cells of the most melanotic malignant melanomas there can be observed clearly either eumelanosomes or pheomelanosomes. Therefore, two types (Type A and Type B) have been distinguished. One typical tumour cell of both types has been characterized ultrastructurally. A retrospective analysis of electron microscopically investigated melanomas which have been ascertained histologically shows that tumours containing eumelanosomes are more frequent than those with pheomelanosomes.

Humans↗
PubMed1981

[Genesis and differentiation of interdigitating cells in human lymph nodes (author's transl)].

Genesis and differentiation of so-called interdigitating cells (IDC) of secondary lymphatic organs are reported by electron microscopic investigation of human lymph nodes in different cutaneous disorders. The formation of these cells apparently take place by differentiation of monocytic cells, in which a cyto-morphological transition into typical IDC--showing characteristic tubulo-vesicular structures--has been observed. Therefore, the IDC apparently represent a special structural and functional form of the monocytic-macrophagic system in lymph nodes and spleen. A hypertrophic reaction of these cells obviously takes place parallel with a decrease of electron density of the cytoplasm as well as an increase of the interdigitating cytoplasmic processes. Differentiated IDC also contain numerous T-lymphocytes within of the cytoplasm, which are partly situated quite near of the nucleus. However, the enclosed lymphocytes show no ultrastructural alterations. Therefore such "emperipolesis" may probably be a normal histo-morphological phenomenon of the lymph nodes whereas in immuno-pathological conditions an accumulation of tubulovesicular structures as well as a decrease of the cytomorphological contacts between IDC and T-lymphocytes has been observed.

Axilla↗
PubMed1980

Ultrastructural differences of interdigitating cells in human lymph nodes.

Eleven axillary lymph nodes from patients with different cutaneous disorders (systemic scleroderma, atopic eczema, psoriasis, hairy cell erythroderma, dermatopathic lymphadenitis) were examined by electron microscopy. In systemic scleroderma interdigitating cells (IDC's) showed typical ultrastructural features as well as intimate contacts with neighboring lymphocytes. In atopic eczema IDC's were characterized by widespread invaginations of the cell membrane, and an increase in tubulo-vesicular structures and microfilaments. Similar observations have been made in dermatopathic lymphadenitis. In psoriasis and hairy cell erythroderma. IDC's showed only a few interdigitations and invaginations of the cell surface. It is supposed that these structural changes in IDC's reflect the different immunological conditions of the diverse cutaneous disorders.

Dermatitis, Atopic↗
PubMed1979

[On the clinical aspects and ultrastructure of bullous pemphigoid (parapemphigus) of the oral mucous membrane (author's transl)].

Bullous changes in the oral mucosa are often the first signs of a general dermatological disease. Successful diagnosis of such diseases is largely dependent upon the use of complex laboratory methods. Clinical and ultrastructural changes are described and discussed with particular reference to a case of parapemphigus. Atypical intracellular vacuolation and marked acantholysis observed in electron micrographs is interpreted as a secondary phenomenon of direct blistering.

Aged↗
PubMed1979

[Fabry disease: clinical, biochemical and electron microscopical studies (author's transl)].

Biochemical investigations on blood-plasma and serum as well as well as electron microscopic investigations on skin lesions biopsy specimens were carried out in one case of morbus Fabry 35 years of age distinctly showing cardio-renal symptoms. The diagnosis of Fabry disease was made when the patient was hospitalized for treatment of a chronic glomerulonephritis. The patients' serum alpha-galactosidase activity was reported to be 10 to 13% of normal controls, whereas the values of trihexosyl-ceramids for blood-plasma and serum were increased. Electron microscopic studies revealed that the endothelial cells and pericytes of the cutaneous vessels as well as the fibroblasts contain numerous liposome-like cytoplasmic inclusions of different size showing an internal lamellar structure with exact periodizity of 65 A. These inclusion are not surrounded by a membrane structure, however, it is possible that the arise from defect lysosomes which accumulate trihexosyl-ceramids owing to insufficient of alpha-galactosidase activity.

Adult↗