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Biomedical subjects

H Klug

Publications and source records attributed to H Klug.

At least 19 recordsLinked to original sources
PubMed1990

[Percutaneous absorption of hematoporphyrin derivative--fluorescence- and electron microscopic studies].

Using surviving human skin, percutaneous absorption following topical application of hematoporphyrin derivative was investigated. Hematoporphyrin derivative gave strong red fluorescence, which was dependent from it's actual concentration, from the used kind of vehicle, from preparation time, and from time between preparation and application of hematoporphyrin derivative solution. Ultrastructural investigation verified cytoplasmatic membranes and mitochondria as the principal targets also after topical application followed by irradiation with visible light.

Hematoporphyrin Derivative↗
PubMed1989

Different ultrastructural feature of keratinocytes in human malignant melanomas.

The ultrastructural feature of keratinocytes was investigated in human superficially spreading melanomas of type B. The tumor cells of these melanomas are characterized by spheroidal melanosomes and containing pheomelanins. Contrary to type A melanomas the keratinocytes show no striking transformation reaction neither in the nucleus nor in the cytoplasm although this part of the cell mostly contains abundant melanin granules surrounding the nucleus. Whether there exists a relation to the clinical course still has to be clarified.

Epidermis↗
PubMed1989

[The ultrastructure of interdigitating cells in atopic eczema].

Electron microscopy was used in investigations of interdigitating reticulum cells of axillary lymph nodes sampled from patients with atopic eczema in different clinical stages. The ultrastructure of these cells was found to differ clearly from that of cells under largely normal conditions. For example, the lumina of the so-called tubulovesicular structures (TV) were clearly reduced. Considerable lobulation of peripheral cytoplasm was an additional finding recorded from the acute clinical stage, while in terms of cellular morphology (interdigitation) contact with T-lymphocytes was obviously reduced. Cytoplasmic processus were clearly decreased in the chronic stage.

Axilla↗
PubMed1988

Unusual mitochondrial reaction in psoriatic keratinocytes.

Electron microscopic investigation of skin biopsies revealed that keratinocytes in clinically uninvolved skin from psoriatic patients show the same mitochondrial ring phenomenon after the application of dithranol as has been observed in the cutaneous lesions proper. In various other skin diseases no mitochondrial reaction was evident after dithranol treatment, nor was it seen in chronic dermatitis when dithranol was not applied. It appears, therefore, that this morphological phenomenon is specific to psoriasis and may provide information concerning functional alterations in the pathogenesis of this disease.

Adult↗
PubMed1988

Ultrastructural features of Langerhans cells in clinically uninvolved skin of psoriatics.

Epidermal Langerhans cells (LC) from skin of clinically normal appearance in psoriatics were studied electron microscopically and compared with LC from the skin of healthy volunteers and from those with other dermatoses. Results show that LC from psoriatics display partly distinct cytoplasmic alterations. Moreover, about half of LC investigated were partly or mainly surrounded by clearly widened intercellular spaces, such that an immediate cellular contact with neighbouring keratinocytes was correspondingly decreased. Whether such LC are functionally altered remains to be clarified.

Humans↗
PubMed1984

Ultrastructure of interdigitating cells in the human tonsil.

Electron microscopic investigations were performed on palatine tonsils from 12 patients with different disorders. In the tonsils a number of non-lymphatic cells were observed which showed the characteristic features of interdigitating cells (IDC): Interdigitating arrangement of the peripheral cytoplasm and invaginations of the cell membrane; Cells with typical tubulovesicular structure, and Partly an electron-lucent cytoplasm. A difference in functional phase may explain the ultrastructural feature of the IDCs. The nucleus, usually containing one or two nucleoli, is mostly multilobulated. These cells are frequently surrounded by mononuclear cells showing ultrastructural characteristics of T-lymphocytes.

Cell Nucleus↗
PubMed1984

Cellular transformation phenomenon in human malignant melanomas.

A cellular transformation phenomenon in keratinocytes based on electron microscopic studies of human cutaneous malignant melanoma is described. In contrast only minimal reactions of this kind were observed in lentigo maligna. The histologic features of the transformation were manifest first by striking enlargement of the nucleus and nucleolus as well as by changes in chromatin structures and the formation of nuclear bodies. The cytoplasmic reaction began with degradation at desmosomes and tonofilaments. A subsequent increase in the number of mitochondria was noted. Moreover, the cytoplasm contained numerous melanosomes, so many in fact that these cells were indistinguishable in their ultrastructure from true tumor cells. In addition to melanosomes, it is quite possible that nuclear substances, which may be responsible for the transformation, are transferred to the neighbouring keratinocytes.

Cell Transformation, Neoplastic↗