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Biomedical subjects

H J Hansen

Publications and source records attributed to H J Hansen.

At least 145 records · Page 8Linked to original sources
PubMed1984

Energy dispersive X-ray analysis of tissue gold after silver amplification by physical development.

Rats were treated intraperitoneally with the gold-containing compounds sodium aurothiomalate (Myocrisin), sodium aurothiosulfate (Sanocrysin), and aurothioglucose. Using stem energy dispersive X-ray analysis, gold and silver were shown to be located at the same point in lysosomes of proximal tubular cells of the kidney, in hepatocytes and in macrophages of lymph glands, spleen and liver. This result indicates that, after exposure to ultraviolet radiation, chemically bound tissue gold is transformed to metallic gold that subsequently can catalyze the reduction of silver ions to silver when subjected to physical development, i.e. exposed to a photographic developer containing silver ions in addition to the reducing molecules.

Animals↗
PubMed1984

The effect of prolonged fasting on total lipid synthesis and enzyme activities in the liver of the European eel (Anguilla anguilla).

The extent of fatty acid synthesis from [1-14C]acetate in liver slices was reduced 6-fold when eels were fasted for 1-7 weeks and 20-fold when fasted for 39 weeks; thereafter hepatic lipogenesis seemed to remain constant for up to 95 weeks of fasting. After a 1-3 week fast some hepatic enzyme activities were reduced (acetyl-CoA carboxylase decreased 2-fold and fatty acid synthetase declined 5-fold), while others remained unchanged (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, alpha-glycerol phosphate dehydrogenase as well as malic enzyme and ATP-citrate lyase). The optimum temperature for measuring both total lipid synthesis and lipogenic enzyme activity in eel liver was found to be 30 degrees C.

ATP Citrate (pro-S)-Lyase↗
PubMed1984

Venous thrombectomy for iliofemoral thrombosis. Early and long-term results in 46 consecutive cases.

The results of 46 consecutive operations for iliofemoral venous thrombosis are presented. Phlegmasia cerulea dolens with impending venous gangrene was present preoperatively in 21 of the 46 patients and pulmonary embolism was suggested by preoperative perfusion scintigraphy in at least 10 patients. There was no surgical mortality. In no case was there additional pulmonary embolism during or after the operation. At follow-up 5 months postoperatively, 44% of the patients were asymptomatic. After a mean observation period of 10 years the corresponding figure was 40%. Surgical management of venous iliofemoral thrombosis should be considered for patients with impending venous gangrene.

Adolescent↗
PubMed1983

Estimation of levels of IgG to multiple sclerosis specific brain antigens in the cerebrospinal fluid of MS patients.

The binding of partially purified multiple sclerosis (MS) specific brain antigens (MSG2) and of the corresponding antigens of non-MS brains (KG2) to cerebrospinal fluid IgG of patients with MS and other neurological diseases was assayed employing sandwich enzyme linked immunosorbent assay (ELISA). Assay of the antigen-antibody binding revealed that the concentration of MSG2 required for the optimum binding to IgG in the undiluted MS CSFs was lower than that of KG2 in all cases. The index for IgG binding capacity of an antigen (IgBC) was expressed as a ratio of the optical density of the enzymic products in ELISA at the optimal antigen-antibody binding to the lowest concentration of the antigen required for the optimal binding. The IgBC of MSG2 was found to be linearly correlated with the IgG concentration in the CSF of MS patients. These results indicate that IgG with specificity to MSG2 may be present in the CSF of MS patients.

Adult↗
PubMed1983

Influence of temperature, environmental salinity and fasting on the patterns of fatty acids synthesized by gills and liver of the european eel (Anguilla anguilla).

A rise in temperature on the average from 8 to 28 degrees C resulted in an enhanced relative incorporation of [1-14C]acetate into saturated fatty acids in liver tissue. The same effect was seen in gill tissue, but only after incubation in vitro and when the precursor was injected into the eel. When [1-14C]acetate was added to the water in the incubation tank such a "homeoviscous adaption" was not observed in gill tissue. A rise in temperature resulted in less relative incorporation of radioactivity into saturated gill fatty acids. We attribute this difference to a specific labelling of salt transport cells in the gills, due to the labelled precursor itself taking part in biological ion transport when it is added to the water. It would appear that palmitoleic acid plays a special role in the function of the salt transport cell. When [1-14C]acetate was added to the water in the incubation tank there was a significantly enhanced percentage incorporation into saturated gill fatty acids in fresh water relative to sea water. Fasting led to less relative incorporation of [1-14C]acetate into saturated liver fatty acids in vitro.

Acetates↗
PubMed1982

Laboratory evaluation and assistance efforts: mailed, on-site and blind proficiency testing surveys conducted by the Centers for Disease Control.

During the last three years, the Centers for Disease Control (CDC) has conducted: 1) on-site surveys in which trained personnel visited laboratories that had experienced performance problems in the quarterly mailed proficiency testing (PT) program, reviewing the laboratories' analytical procedures by using carefully referenced samples to determine sources of errors and providing assistance in correcting them; 2) special assistance surveys in which carefully referenced samples were mailed to laboratories that had performed unsatisfactorily in routine mailed PT surveys and then telephone consultations were conducted to correct the problems; and 3) blind surveys in which carefully referenced samples were sent through normal patient sample acquisition routes to assess the actual day-to-day performance capability of the laboratories. Results suggest that on-site surveys by trained laboratory surveyors and special mailed assistance surveys can be very effective in identifying the source of analytical errors in laboratories previously found, through mailed PT surveys, to have performance problems. Blind-survey results indicate that good performance in mailed PT does not necessarily imply good laboratory performance with routine patient specimens. Although difficult to conduct, blind surveys should be conducted whenever the logistics can be worked out by contractors for laboratory services, clinicians using laboratory services, and the laboratories themselves to assure the continuation of quality service.

Blood Chemical Analysis↗
PubMed1981

Familial hemiplegic migraine--a reappraisal and a long-term follow-up study.

Six patients (two females and four males in one family) with hemiplegic migraine are described. The age of onset was between six and eighteen years. Hemiplegic attacks were usually accompanied by transient neurological disturbances referable to the territory of the vertebrobasilar arterial tree. Brain-stem involvement and vertebrobasilar dysfunction in the reported family was supported by angiography in one case, revealing a marked and prolonged spasm of the basilar artery. Despite its dramatic character and occasionally prolonged deficits the course was essentially benign. Hemiplegic attacks disappeared during adolescence in five of the six patients. No permanent neurological residual phenomena were encountered during a mean follow-up of 14 years. The possibility is raised of a genetically determined susceptibility to periodic vasoconstriction in a particular vascular area as the basis of the syndrome.

Adolescent↗
PubMed1981

Effect of symptomatic therapy on motor difficulties in quantitative measurements of voluntary power.

Thirty-one multiple sclerosis patients in a stable phase were given intensive symptomatic therapy. Isometric and isokinetic measurements of voluntary power were used to assess the effect of the treatment. Twenty-one of the patients were improved, six unchanged and four aggravated. The relative improvement was on the average 35%, best in the weakest patients. Isokinetic measurements revealed a particularly good progress for the fastest movements, probably reflecting reduction of spasticity. The optimum effect of treatment was found on an average after three weeks. Such tests seem to be useful in following and analysing the training and possibly in establishing treatment models.

Adult↗
PubMed1981

A-beta-lipoproteinaemia. Ocular involvement in a Danish case.

A-beta-lipoproteinaemia in a Danish woman is reported. The ocular involvement comprised pigmentary retinopathy, anisocoria and reduced corneal sensibility. Serum concentrations of vitamin A and retinol-binding protein (RBP) were low, but were normalized after vitamin treatment. Serum concentration of carotene was low, and not altered by treatment. The retinopathy progressed over a 2 1/2 year period in spite of controlled serum vitamin A and RBP concentrations. The possible role of vitamin A in the development of retinopathy is discussed.

Abetalipoproteinemia↗