Biomedical subjects

H Ide

Publications and source records attributed to H Ide.

At least 199 records · Page 11Linked to original sources

PubMed1994

Alcohol consumption and risk of esophageal cancer in Japan: a case-control study in seven hospitals.

In a multi-center case-control study, we evaluated the risk of esophageal cancer in the Japanese population. All patients and controls were inpatients in the surgical departments of seven hospitals nationwide. Patients eligible for the study were those newly diagnosed as having primary esophageal cancer. One control per case was selected from among patients admitted to the same hospital, and 141 male pairs were analyzed using logistic regression analysis. The results showed dose-response relation between the risk of esophageal cancer and both the quantity (g/week) and frequency (times/week) of alcohol drinking (P value for trend = 0.0001). Although a statistically significant risk increase was shown among moderate to heavy smokers (15 < or = cigarette/day < 25) (odds ratio, 4.35:95% confidence interval, 1.81-10.49), the dose-response for cigarette smoking was unclear (P value for trend = 0.07). No combined effect of alcohol drinking and cigarette smoking was found. A frequent intake of fruit was associated with a decreased risk (P value for trend = 0.02). After adjustment for alcohol consumption, cigarette smoking and fruit intake were found not to be associated with the risk, whereas a preference for high-temperature food and drink showed a statistically significant positive association (P value for trend = 0.02). Drinkers who consumed shochu most frequently showed a three-fold increased risk over that for beer consumers, although the association disappeared after adjusting for the amount of alcohol consumed. The present results confirm alcohol intake and a preference for high-temperature food to be associated with an increased risk of esophageal cancer and show the amount of alcohol consumed, rather than the type of alcoholic beverage, to be the main risk determinant.

Alcohol Drinking↗

PubMed1994

[A case of tuberculous pyothorax with retroperitoneal gravitation abscess].

A 65-year-old woman being treated under the diagnosis of pneumonia was subjected to further evaluation for right back tenderness. Chest radiography revealed a granular, net-like shadow in the right lower lung field, and right pleural thickening with pleural fluid retention. Ultrasonography demonstrated a cystic mass of uniformly low echo density, corresponding to the site of tenderness. This lesion was located on the right renal dorsal, right iliopsoas muscle ventral side. Based on CT findings of internal water density and marginal enhancement, the lesion was diagnosed as an abscess. With the suspicion of right tuberculous pyothorax with retroperitoneal gravitation abscess, puncture was attempted. The diagnosis was confirmed by the presence of Mycobacterium tuberculosis in the specimen. Antituberculosis chemotherapy was initiated, with drainage for symptom relief and lesion range definition. The abscess cavity communicated with the right pleural cavity via s stalk. The stalk path was confirmed by CT, and surgical curettage was performed. Tuberculous pyothorax with retroperitoneal gravitation abscess is rare and is not discussed in standard textbooks. In large series of cases it is not mentioned. In this patient we assumed that retroperitoneal gravitation abscess occurred as a result of the advanced state of tuberculous pyothorax.

Abdominal Abscess↗

PubMed1994

Sorting out of limb bud cells in monolayer culture.

To examine differences in the surface properties of limb bud cells, we mixed cells from the progress zone at different stages or from different positions along proximodistal axis of chick and quail wing limb buds. To identify the origin of cells, a chick-specific antibody was used in a mixed culture of chick and quail cells, or cells from one of the stages were labeled with a fluorescent dye, PKH-26. Within 18 hours in mixed culture, cells segregated from each other and formed patches of various sizes. The process zone cells at early developmental stages mixed homogeneously with the cells from proximal region of old limb buds and the progress zone cells at late stages mixed homogeneously with the cells from distal region of the old limb buds. These results suggest that surface properties of cells in progress zone change during limb bud development and vary along the proximodistal and anteroposterior axes of the limb bud and that these differences in surface property may correspond to the positional values for limb pattern formation.

Animals↗

PubMed1994

[Problems and present status of clinical trials for esophageal carcinoma].

This paper reviews clinical trials for esophageal carcinoma, mainly from the results of the JEOG (Japanese Esophageal Oncology Group) study. Preoperative radiotherapy was not conducive to survival of esophageal cancer patients. On the third JEOG trial, postoperative chemotherapy of CDDP+VDS was equivalent to survival as 50Gy of postoperative irradiation. And on the further fourth trial, survival was also equivalent between the two groups treated with surgery alone or postoperative chemotherapy of CDDP+VDS. This fact reveals the in effectiveness of these therapies. The response rate from JEOG phase II study of CDDP and 5-FU was 35.9%, and RCT of surgery alone versus surgery + postoperative chemotherapy with CDDP+5-FU was ongoing as the fifth JEOG trial. Chemo-radiotherapy seemed to be very effective for esophageal cancer patients, and it may provide a complete cure for them in the future.

Antineoplastic Combined Chemotherapy Protocols↗

PubMed1994

[A case of primary angiosarcoma of the heart].

We report a case of angiosarcoma of the right atrium, manifested as a cardiac tamponade. The patient was a 57-year-old man. Transesophageal echocardiographic examination revealed a pericardial effusion and an abnormal structure arising in the right atrium. Emergency operation was performed and the tumor in the right atrium was resected under cardiopulmonary bypass. The resected tumor measured 5.0 x 3.5 x 2.0 cm, and microscopic examination revealed an angiosarcoma. The patient was initially recovered, but he died of heart failure caused by the recurrence in the left ventricle on the 5th postoperative month.

Cardiac Tamponade↗

PubMed1994

[Replacement of ascending and proximal transverse aorta for a 90-year-old female with aortic dissection].

Successful replacement of ascending and proximal transverse aorta for a 90-year-old female with aortic dissection was achieved using selective cerebral perfusion with cold blood. The ascending aorta enlarged as a diameter of 7 cm and massive blood flow was recognized in the false lumen detected by CT scanning and echocardiography. After the core cooling by cardiopulmonary bypass (CPB), the branches of the aortic arch were cannulated and perfused with cold blood (16 degrees C) for cerebral protection. The time of CPB, cardiac arrest and selective cerebral perfusion was 153 min, 92 min and 35 min, respectively. Post operative course was uneventful. She discharged from the hospital 4 weeks after the operation.

Aged↗

PubMed1994

Evaluation of the pulsatility of a new pulsatile left ventricular assist device--the integrated cardioassist catheter--in dogs.

A new pulsatile left ventricle-femoral artery bypass system (integrated cardioassist catheter system) has been developed for rapid, percutaneous insertion as a left ventricular assist device. Previous experiments revealed its superiority over the intraaortic balloon pump system in maintaining the peripheral circulation and in improving myocardial blood flow and afterload. Our objective was to determine whether the pulsatility of left ventricular bypass of this system would be preferable for maintaining the peripheral circulation and managing the ischemic myocardium as compared with nonpulsatile left ventricular bypass. Ten dogs with profound heart failure were supported by this system. Their hemodynamic status and myocardial blood flow were measured under control, nonpulsatile left ventricular bypass, or synchronous pulsatile left ventricular bypass. Significant differences between the nonpulsatile bypass group and the pulsatile bypass group were observed in the mean increase in aortic pressure (3.5% versus 22.2%, respectively; p < 0.001), total cardiac output (13.0% versus 21.7%; p = 0.004), and myocardial blood flow (9.5% versus 21.8%; p < 0.001). No differences were found between groups in the decrease in left atrial pressure (-20.2% versus -20.2%; p > 0.05). The ratio of diastolic time index/tension time index was shown to be improved significantly in the pulsatile bypass group compared with that of control and nonpulsatile bypass groups (p < 0.001). Thus, the pulsatility of the integrated cardioassist catheter system may support the peripheral circulation and improve the myocardial blood flow and oxygen supply/demand ratio.

Animals↗

PubMed1994

[Application of PCPS (percutaneous cardiopulmonary support) to the resuscitation of DOA (dead on arrival) patients].

We report three cases of DOA patients who were resuscitated by using PCPS. All had resisted standard advanced life support methods. The conditions responsible for the heart arrest were acute myocardial infarction (AM1) in 2 (case 1 and 3) and subarachnoid hemorrhage (SAH) in the other (case 2). All 3 were resuscitated by PCPS. Case 1 survived with a slight neurological deficit. Case 2 succumbed to SAH 3 days later. Case 3 was transferred to left heart bypass but succumbed to hemorrhagic infarction of the brain 6 days later. Although this system allows rapid and simple deployment and provides a benefit in resuscitation of DOA patients in a state that is refractory to standard cardiopulmonary resuscitation, the problem of indication for resuscitation remains to be solved.

Angioplasty, Balloon, Coronary↗

PubMed1993

Formation of distal structures from stumps of chick wing buds at stages 24-25 following the grafting of quail tissue from X-irradiated distal limb buds.

To examine the regulatory activity of the proximal region of chick limb buds, distal halves of chick wing buds at stages 24-25 were removed, and the distal tips of X-irradiated stage 21 quail limb buds with an apical ectodermal ridge (AER) were grafted onto the chick stumps. The host stumps formed only humerus after removal of the donor distal tips or with tip mesoderm alone. However, when distal tips with an AER were grafted onto the stumps, one or two cartilage elements of host origin were formed at the distal end of the humerus. To examine whether the stump cells have changed to cells of a more distal, progress zone (PZ) cells, the chick stumps with quail tips were immunostained with antibody against AV-1, which reacts with the anterior PZ region of chick limb buds and is specific for an antigen that is expressed under the control of the AER. Within two days of grafting, cells positive for the antigen reappeared in the stump tissues. These results suggest that the some of the stump cells may be converted to PZ cells and their positional values may change to those of more distal structures under the influence of the AER.

Animals↗

PubMed1993

Synthesis and damage specificity of a novel probe for the detection of abasic sites in DNA.

The abasic site (apurinic/apyrimidinic site) is the most common lesion in DNA and is suggested to be an important intermediate in mutagenesis and carcinogenesis. We have recently reported a novel assay for the detection and quantitation of abasic sites in DNA [Kubo, K., Ide, H., Wallace, S. S., & Kow, Y. W. (1992) Biochemistry 31, 3703-3708]. In this assay, the aldehyde group in an abasic site is first modified by a probe bearing a biotin residue, called the Aldehyde Reactive Probe (ARP) and then the tagged biotin is quantified by an ELISA-like assay. However, in the previous study, ARP was prepared only in a crude form, and no solid chemical data concerning the structure and specificity of ARP were reported. In this study, an improved method for the preparative synthesis of ARP has been established, and its structure has been unambiguously characterized using spectroscopic means. In order to elucidate the specificity of ARP to DNA damages, ARP was incubated with a variety of damaged bases or nucleosides and the reaction mixtures were analyzed by HPLC. Of the 14 compounds tested for their reactivity to ARP, 2-deoxyribose (a model compound for an abasic site) and 5-formyluracil reacted with ARP. Interestingly, compounds bearing a formamide group such as formamidopyrimidine and deoxyribosylformamide did not react with ARP, indicating that ARP is specific to damages having an alkyl or allyl aldehyde group. Furthermore, the ability of ARP synthesized by the defined chemical route to detect abasic sites has been substantiated using natural DNA containing abasic sites. Potential applications and limitations of the ARP assay are discussed.

Animals↗

PubMed1993

Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112.

The complete amino acid sequence of transglutaminase (EC 2.3.2.13) (TGase), which is produced by a microorganism, Streptoverticillium sp. strain s-8112, and catalyzes the acyl transfer reaction between gamma-carboxyamide groups of glutamine residues in proteins and various primary amines, has been established by a combination of fast atom bombardment mass spectrometry and standard Edman degradation of peptide fragments produced by treatment of the TGase with various proteolytic enzymes and purified by a reversed-phase high performance liquid chromatography. The TGase consists of 331 amino acid residues with a chemical molecular weight of 37,863, in agreement with the observed molecular weight (37,869.2 +/- 8.8) determined from its electrospray ionization mass spectrum. The sequence of the enzyme is very different from those of mammalian TGases represented by guinea pig liver enzyme. The enzyme contains a sole Cys residue, which is essential for its catalytic activity. Hydropathy analysis indicated that the secondary structure of the region around the active site Cys residue is similar to those of mammalian TGases. These results suggest that this microbial protein evolved by a different pathway from that of mammalian TGases and acquired acyl transfer activity during the evolutional process.

Amino Acid Sequence↗

PubMed1993

Investigation of the interaction between enzyme and inhibitor by the combination of chemical modification, electrospray ionization mass spectrometry and frit-fast atom bombardment liquid chromatography/mass spectrometry.

The interaction between enzyme and its inhibitor, hen egg-white lysozyme and tri-N-acetylglucosamine (NAG3), was studied by the combination of chemical modification, enzymatic digestion, electrospray ionization mass spectrometry and frit-fast atom bombardment liquid chromatography/mass spectrometry. Chemical modification of amino groups, carboxyl groups, and indole groups was carried out independently. In the absence of NAG3, the carboxyl group in Asp 101 was modified by glycinamidation, and the indole group in Trp 62 was modified by Koshland reagent. In the presence of NAG3, the degree of modification of Asp 101 and Trp 62 decreased. It is suggested that Asp 101 and Trp 62 are involved in the interaction with NAG3. The result is consistent with the one obtained by x-ray crystallography. It is indicated that the combination of chemical modification and mass spectrometry may be effective for the investigation of the binding reaction of enzyme to inhibitor and of protein-protein interaction.

Amines↗

PubMed1993

Expression of AV-1, a position-specific molecule, in response to retinoic acid beads and ZPA grafts in chick limb development.

Local application of retinoic acid (RA) to the anterior-distal margin of chick limb buds causes digit pattern duplications identical to those that result from zone of polarizing activity (ZPA) grafts. The AV-1 antigen is a cell membrane glycoprotein which is expressed during chick limb bud development in a stage- and position-specific manner that correlates with cartilage pattern formation. In order to further understand the limb patterning mechanisms affected by RA and by ZPA grafts, we first examined the effects of RA treatment on AV-1 expression. We then compared the effect of RA with those of ZPA grafts. When beads soaked in varying concentrations of RA were applied to the anterior-distal margin of wing buds, changes in the pattern of AV-1 antigen expression were observed. AV-1 expression appears to correlate with regions that will give rise to a digit 3. These results suggest that the AV-1 protein is one of the molecules which may be involved in the process of cartilage pattern formation during limb development. ZPA grafts produced similar alterations in AV-1 expression, but the initial changes were observed earlier with ZPA grafts than with RA beads. These results support the idea that RA changes anterior limb bud cells into ZPA cells.

Animals↗

PubMed1993

Induction of polarizing activity by retinoic acid occurs independently of duplicate formation in developing chick limb buds.

Retinoic acid (RA) is known to mimic the action of the zone of polarizing activity (ZPA) in inducing the formation of anteroposterior duplicates in the chick limb bud. Although RA had been thought to be a morphogen produced by the ZPA, recently we (Noji et al., 1991) and Wanek et al. (1991) independently concluded that RA induces polarizing activity in cells at the anterior margin of the chick limb bud. In this study, we examined the distribution of RA-induced polarizing activity in the limb bud. At first, RA-containing beads were implanted into various regions of stages 20-23 limb buds. After 24 hr, we grafted tissue fragments from sites adjacent to the RA beads into the anterior margin of host limb buds. High polarizing activity was induced in anterior-distal and apical mesoderm regions immediately under the apical ectodermal ridge (AER) when beads presoaked in 1 mg/ml RA were grafted to anterior sites at stages 19-20. However, in mesoderms distant from the AER, even if adjacent to the implanted RA bead, only low activity was induced. Although implantation of beads soaked in a high concentration (10 mg/ml) of RA did not result in duplications but rather in truncations of the limbs, the apical mesodermal cells in these limbs were converted to ZPA cells, indicating that induction of polarizing activity by RA can occur independently of duplicate formation. The ability to respond to RA treatment was rapidly lost in the cells along the anterior margin of stage 22 limb buds. When the anterior AER was removed, the induction of polarizing activity in the anterior margin of limb buds was markedly reduced. Thus, the AER seems to be necessary for the RA-directed induction of polarizing activity.

Animals↗

PubMed1993

Basic FGF maintains some characteristics of the progress zone of chick limb bud in cell culture.

The apical region of chick limb buds (progress zone, PZ) is known to be essential for limb pattern formation. In the PZ, the cells are in an uncommitted state and change their positional values under the influence of the apical ectodermal ridge (AER). Using a culture system of chick PZ cells, we have examined the factor(s) which helps to maintain some of the characteristics of PZ cells. It was found that basic FGF (bFGF) stimulated the expression of AV-1 and Msx 1 mRNA, which are rich in PZ cells at early stages, in the cultured PZ cells. These two molecules were rapidly lost in the control culture without the bFGF. Further, bFGF promoted growth and inhibited chondrogenesis of the cultured PZ cells. When quail PZ cells were cultured in FGF medium, harvested, and grafted to the apical region of chick limb buds, in about half of the grafts, the cells distributed to form a long band along the proximodistal axis and participated in normal host cartilage pattern formation as did intact PZ tissues. In contrast, those cultured in the medium without bFGF distributed as a cluster and did not participate in host cartilage pattern formation. The culture with conditioned medium of BRL cells increased the percentage of the grafts participating in normal pattern formation. These results indicate that bFGF helps to maintain some of the characteristics of PZ cells in culture and suggest that bFGF may be a PZ maintenance factor from the AER.

Animals↗

PubMed1993

MRC-5, human embryonic lung fibroblasts, induce the duplication of the developing chick limb bud.

The anteroposterior (A-P) axis pattern of the chick limb is likely to be controlled by a small region of mesenchyme cells at the posterior margin of the limb bud (ZPA, zone of polarizing activity). In this study, we found that MRC-5 fibroblast cells had the capacity for duplicated-pattern formation of the chick limb along the A-P axis when grafted to the anterior region of the limb bud. MRC-5 cells were effective only during pre-limb bud stages, and the leg bud was more responsive than the wing bud. Grafted cells remained at the base of the limb bud when limb development proceeded. These results suggest that the products of MRC-5 cells are involved in three possible processes of duplicated-pattern formation: induction of the polarizing activity; maintenance of this activity, which is present weakly at pre-limb bud stages; and determination of A-P axis as the ZPA factor(s). By allowing the use of a small number of cells of embryonic tissues with polarizing activity, the analysis of duplicate formation with the MRC-5 cell line provides a powerful tool for elucidating the molecular nature of the ZPA.

3T3 Cells↗

PubMed1993

Formation of ductular structures in vitro by rat pancreatic epithelial oval cells.

In the rat pancreas oval cells were shown to contain albumin transcripts and are considered to be precursors of pancreatic hepatocytes. Pancreatic oval cells have been maintained in our laboratory as a cell line. In the present study we have examined the differentiation potential of rat pancreatic epithelial oval cells under different culture conditions using light and electron microscopy and in situ hybridization techniques. Oval cells cultured on collagen gels formed a monolayer. However, when oval cells were embedded in collagen gels and cultured with or without fibroblasts, they readily organized into duct-like structures within 1 week. Over the next 3 weeks there was a gradual increase in the number and size of ductules. Morphological examination of duct-like structures showed true lumen formation. Ultrastructurally, cells showed features of ductal cells characterized by well-formed cell junctions, interdigitation of plasma membranes, well-developed cytoplasmic organelles, and apical microvilli. Light microscopic autoradiography showed proliferation of cells lining the ductules throughout the 4-week culture period. Albumin mRNA expression was demonstrable by in situ hybridization with albumin probe in oval cells cultured on collagen gels and embedded in collagen gels. Results of these studies indicate that under these in vitro conditions, oval cells appear to express albumin transcripts but exhibit duct-like morphogenetic features.

Animals↗

PubMed1993

Successful repair of combined ventricular septal rupture and free wall rupture.

The successful surgical repair of combined ventricular septal perforation and free wall rupture (double rupture) after inferior myocardial infarction is reported. Extensive inferior wall infarctectomy was performed, and both defects were closed with a prosthetic patch, using a modification of the technique developed by Daggett. The patient was alive and well 1 year after operation.

Heart Rupture, Post-Infarction↗