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Biomedical subjects

H Huber

Publications and source records attributed to H Huber.

At least 235 records · Page 13Linked to original sources
PubMed1984

Immunopathology of non-Hodgkin lymphomas.

The immunopathological features of lymphocytic non-Hodgkin lymphomas (NHL), follicular centre cell tumours and "large-cell" NHL are described with particular emphasis on in situ evaluation of cryostat sections using monoclonal antibodies. The immunological phenotypes of tumour cells and the pattern of "reactive" cellular infiltrates with characteristic differences for the various subtypes are summarized. Immunological membrane features of the following lymphocytic NHL of B lymphocyte origin are discussed in detail: B-CLL (most common phenotype of the lymphoma cells: VIB-C5, To-15, Ia, Leu-1), lymphoplasmocytoid NHL (To-15, Ia), B-prolymphocytic leukaemia (VIB-C5, To15, Ia, Leu (+/-), FMC-7(+/-] and hairy-cell leukaemia (HCL: To-15, Ia, FMC-7). NHL of T-lymphocytes detailed in respect of mycosis fungoides and Sézary syndrome (OKT-3, OKT-4, OKT-11), to subtypes of T-CLL with particular emphasis on the "azurophilic" type, to T-zone NHL, and to adult T-cell leukaemia/lymphoma (OKT-3, OKT-4, OKT-11, anti-tac), all of them with the usual immunological phenotype of T-helper (TH) lymphocytes. NHL with a predominance of T-suppressor (TS) lymphocytes include the immunoblastic lymphadenopathy like T-cell lymphoma (OKT-3, OKT-8, OKT-11) and some lymphoepitheloid (Lennert) lymphomas. Immunological features of follicular centre cell tumours are discussed with emphasis on similarities and differences with the normal germinal centre. Tumour cells usually show the following membrane phenotype: centroblastic/centrocytic (cb/cc: To-15, VIL-Al, Ia; meshwork of non-neoplastic R4/23+, To-5 + DRC), centrocytic (VIB-C5, To-15, Leu-1, Ia; R4/23 and To-5 meshwork), centroblastic (cb, To-5, To-15, Ia, VIL-Al +/-). "Large-cell" NHL include the last entity (cb), immunoblastic (B-ib, T-ib) and lymphoblastic (lb) NHL and are discussed in respect to B-ib (Ia, To-15 +/-, VIL-Al +/-), T-lb (WT-1, OKT-6 +/-, OKT-11 +/-), pre-B-lb (VIB-C5, Ia, TdT, VIL-Al +/-) and Burkitt-type NHL (VIB-C5, VIL-Al, Ia, sIg). "Reactive" cellular infiltrates may account for a large fraction of the cellular content of NHL (e.g. the mean TH and TS content amount to about 40% of that seen in normal lymph nodes). In comparison to other NHL B-CLL and cb/cc showed the highest, HCL the lowest number of TH X TS exhibited less typical alterations.(ABSTRACT TRUNCATED AT 400 WORDS)

Antibodies, Monoclonal↗
PubMed1984

Subpopulations of colony-forming B lymphocytes exhibit distinct in vitro growth characteristics.

Human peripheral blood cells enriched for B lymphocytes were stimulated to focal proliferation in semisolid cultures with lymphocyte-conditioned media, Protein A (Prot A), lipopolysaccharide (LPS) and 2-mercaptoethanol (2-ME). After 6-8 days of incubation, two morphologically distinct colony types were observed. Type I colonies were diffusely proliferating aggregates within the agar layer, whereas another subset of B-cell-colony-forming cells (CFU-BL) formed round compact type II colonies which appeared to leave the agar layer and continued to proliferate in the liquid overlayer of our culture system. They reached maximum proliferation 2 days earlier than type I colonies. Cells derived from both colony types were positively identified as B lymphocytes by monoclonal antibodies using immunoperoxidase staining. In addition to this distinct growth pattern, both colony types exhibited different proliferative responses which were dependent on the kind of conditioned media used and the mitogen concentration. In secondary cultures both type I and type II colony-derived cells showed recloning capacity. However, after replating, both colony types gave rise to round compact type II colonies. These results demonstrate that there exist at least two subpopulations of colony-forming B lymphocytes, possibly one more primitive than the other, which can be distinguished by in vitro growth characteristics.

B-Lymphocytes↗
PubMed1984

Radiosensitizing effect of medroxyprogesterone acetate on endometrial cancer cells in vitro.

From clinical experience it is known that medroxyprogesterone acetate (MPA) can increase the radiosensitivity of adenocarcinomas of the corpus uteri. This study investigates this phenomenon in vitro. Primary explants of highly differentiated adenocarcinomas were irradiated with or without pretreatment with MPA and compared with an untreated control group and to a group treated with MPA only. Cell culture itself was performed on an agarose medium in order to prevent overgrowth by fibroblasts. Untreated samples formed 43 +/- 5 clones, explants treated with MPA only produced 39 +/- 5 clones, a difference which was not statistically different; samples irradiated without pretreatment produced 16 +/- 8 and samples after combined treatment 9 +/- 3 clones (all values means +/- SD). This numeric reduction of cell growth through preirradiation treatment with MPA was statistically significant. The effect of MPA as a radiosensitizer may be due to its potential to prolong the radiosensitive G2 phase of the cell cycle. This effect of MPA may be useful also in other hormone-dependent tumors.

Adenocarcinoma↗
PubMed1984

Imbalance of helper and suppressor T lymphocytes in malignant non-Hodgkin lymphomas: an in situ morphometric analysis.

The number and distribution of reactive T cells within 100 malignant B-cell lymphomas were evaluated in situ by immunomorphometry using stereological methods. Findings were related to histological and clinical parameters. A mean of 2 X 10(4) reactive T cells/microliter tumour tissue was found. This corresponds to 40% of the T-cell content of normal lymphatic tissues. The distribution of reactive T cells within the tumours was diffuse except for centroblastic/centrocytic lymphomas. When evaluating the different histological entities a correlation between number of helper T cells, T helper:T suppressor (TH:TS) ratio and histological subgroups emerged, particularly in non-Hodgkin lymphomas of low-grade malignancy. The highest ratio was found in prognostically favourable subgroups, CLL (2.7 +/- 0.3) and tumour areas of centroblastic/centrocytic lymphomas (2.9 +/- 0.4). In contrast, a significantly lower ratio was found in centrocytic lymphomas (1.4 +/- 0.3) corresponding well to the worst prognosis of this subgroup. The relationship between the number of helper T cells in tumour tissues, TH:TS ratio and prognosis was confirmed and extended by the evaluation of clinical data. It could be shown that, independently of histological criteria, a close correlation exists between the number of T cells, particularly T helper cells within the tumour, TH:TS ratio and clinical course. Patients with a favourable course had 1.4 X 10(4) T helper cells/microliter tumour tissue compared to only 0.8 X 10(4) for patients with an unfavourable clinical course (p less than 0.01); the TH:TS ratio was 2.8 for the favourable and 1.8 for the unfavourable group, respectively (p less than 0.04). In contrast, neither treatment nor tumour stage had a clear-cut influence on the extent of T-cell infiltration.

B-Lymphocytes↗
PubMed1984

[Additive therapy in ovulation induction with clomiphene].

Between 1977 and 1981 1319 patients consulted our hospital with the problem of sterility. 763 women became pregnant, 200 were treated with clomiphen . In 69 cases we used a combination with other agents, because ovulation could not be induced with clomiphen alone. 7 times we combined clomiphen with HCG, in 7 other cases with Prednisolon . Ovulation and pregnancy occurred subsequent to a treatment with Estradiolvalerianate -Norgestrel and clomiphen in 15 patients, in 32 patients after administration of a combination of clomiphen and Bromocriptine. The inadequate cervical mucus which may develop in patients during clomiphen therapy was treated with an additional low dose of ethinylestradiol. Our retrospective study confirms the value of a combination therapy in the treatment of anovulation and sterility.

Adult↗
PubMed1984

Experiences in the application of NONMEM to pharmacokinetic data analysis.

Ethical issues arising from the use of patients in medical research have stimulated pharmacokinetic research in population kinetics, which requires only a few concentration samples of each individual. Using historical maprotiline data, the new approach of population kinetics was investigated and compared to individually estimated kinetics. Two different population kinetic methods were applied. The naive approach, a quick and dirty method, was compared to the nonlinear mixed-effects method, which was applied by the NONMEM package. Based on the results we obtained from actual maprotiline data as well as from simulated data, NONMEM is a reasonable tool for the estimation of population pharmacokinetic parameters. The main advantage of NONMEM over the naive approach lies in the possibility of obtaining standard deviations of random effects related to the variability between subjects.

Computers↗
PubMed1984

[Bleomycin-induced linear hyperpigmentation].

We describe the progression of bleomycin-induced inflammatory lesions to linear hyperpigmentations in 2 patients. Striking, hitherto unreported observations were the development of streaking after a single dose of 5 mg bleomycin (patient 1) and vacuolar degeneration of melanocytes in the inflammatory stage (patient 2).

Bleomycin↗
PubMed1984

Human large granular lymphocytes and their relationship to natural killer cell activity in various disease states.

This study investigated the numbers of large granular lymphocytes (LGL) and their relationship to natural killer (NK) function, as assessed by their capacity to lyse the human tumor target, K562. Peripheral blood mononuclear cells from 42 normal controls and from 171 patients suffering from various nonmalignant or malignant diseases were evaluated. Also studied were samples from a patient undergoing autologous bone marrow reconstitution following total body irradiation. Results suggested the existence of a close relationship between the numbers of LGL and the capacity to lyse K562 targets, further supporting the view that LGL are crucial effector cells mediating NK lysis. In certain diseases, such as malignant states, functional capacity was not simply determined by the numbers of LGL. Here preferential reduction of the capacity to lyse K562 targets was observed, indicating that additional limiting factors are involved in the determination of the cytotoxic potential. Based on the relationship between LGL and natural immune functions, as well as on the identification of leukemias affecting this cell type, we would recommend their evaluation on a large scale clinical basis.

Adolescent↗
PubMed1984

[Radiomenolysis of the endometrium using high-dose iridium irradiation--clinical and cytologic results].

423 patients with benign recurrent uterine bleeding resistant to other therapy were treated by intracavitary radiotherapy. In all cases there was a contraindication for the operative removal of the uterus. Since 1980 radiomenolysis was performed by high-dose Iridium-192 irradiation (afterloading technique). In 94.3% of our cases with Radium-226 irradiation the bleeding could be treated successfully. Side effects of the irradiation occurred only in a minimal and neglectible percentage. In all cases irradiated with Iridium-192 the bleeding stopped after treatment. Side effects could not be observed. Therefore the intrauterine contact-irradiation therapy with high-dose Iridium-192 should be remembered in cases of uterine bleeding resistant to hormonal therapy or in cases of high risk for operation. With the afterloading device the molestation for the patients could be reduced to a minimum. By intrauterine exfoliative smear performed before and after irradiation the cytomorphologic effect of an Iridium-192 afterloading irradiation was demonstrated. The examinations showed that the cytologic findings were characteristic for the effect if ionizing radiation of the histologically unchanged, not radiosensitive, normal cylindrical epithelium. A marked cellular diathesis and augmentation of size of nuclei and plasma is a characteristic result of the irradiation with Iridium-192.

Adult↗
PubMed1983

[Molecular-biological studies of the cervical secretion with one-dimensional electrophoresis].

In the present study the polypeptide composition of cervical mucus was analysed during the human menstrual cycle. Changes in the polypeptide profile as displayed by a very sensitive silverstaining method were consistent with cycle dependent variations in observed rheological properties of the mucus and activities (concentrations) of luteinising hormone (LH) (from comparatively analysed blood). It could be shown that whereas three main polypeptide components of the mucus (50 kd [kilodalton], 14 kd, 12 kd) remained constant the amount and intensity of other polypeptides increased towards midcycle (ovulation) and diminished on the days after ovulation. A 25 kd polypeptide (being a main component on the days before ovulation) disappeared at midcycle (on the day of ovulation). On the same day a new main peptide (8 kd) was observed, which was decreasing (in favour of the 25 kd peptide) on the days after ovulation. It is postulated that the described shift in the polypeptide pattern of the cervical mucus may be a result of the degradation of the light chain of IgG at midcycle.

Cervix Mucus↗