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H Huber

Publications and source records attributed to H Huber.

At least 217 records · Page 12Linked to original sources
PubMed1987

Diagnostic and prognostic value of immunohistological bone marrow examination: results in 212 patients with lymphoproliferative disorders.

Cryostat sections of 246 consecutive bone marrow biopsies from 212 patients with lymphoproliferative disease were investigated using a panel of monoclonal antibodies (MOAb's) and an immunoperoxidase technique. Bone marrow involvement was demonstrated by immunohistological examination in 121/160 patients (76%) with non-Hodgkin lymphomas (NHL) and 16/23 patients (70%) with plasma cell malignancies; the definite immunological diagnosis could be performed in 77% and 88%, respectively. Reactivity with the MoAb Ki-67 correlated with clinical parameters: in all cases exhibiting more than 5% positive cells an unfavourable course was seen, independent of the histological subtype. Another MoAb of potential prognostic relevance is KiM 4 b, which reacts with follicular dendritic cells (FDC). Besides the presence of FDC in germinal center tumors (CB/CC and CC-NHL) we found FDC in a minority of cases with B-CLL (5/44) and IC lymphoma (4/18). In the latter group 3/4 patients showed a favourable clinical course (vs 2/14 without FDC). The MoAb Tü 1 could discriminate between the lymphoplasmocytoid (11/12 positive) and the lymphoplasmocytic (0/6 positive) subtype of IC lymphoma and has proven of diagnostic importance. Expression of IL-2 receptors, detected by MoAb anti-Tac (CD 25), was demonstrated on leukemic cells from patients with hairy cell leukemia (100%), B-CLL (82%), IC (61%), CC (50%) and CB/CC lymphoma (50%). A considerable number of reactive T-lymphocytes (5%-60% of tissue cells) were identified among the neoplastic B cells with a predominance of CD4+ cells in most cases with NHL, whereas the CD4+/CD8+ ratio was significantly lower in myelomas and non-infiltrated bone marrows. The potential meaning of these findings is discussed. The immunohistological bone marrow analysis represents an important additional method in the diagnostic procedures of lymphoproliferative diseases involving the bone.

Antibodies, Monoclonal↗
PubMed1986

[Aortocoronary bypass operation as an emergency intervention after transluminal coronary angioplasty. Which factors prevent the incidence of a major infarct?].

Among 830 patients in whom transluminal coronary angioplasty (TCA) was undertaken there were 41 (4.9%) who required an aortocoronary bypass operation for acute complications (vessel dissection with markedly reduced flow; complete occlusion or contrast-medium extravasation). The group of 41 was divided into two, based on angiography (25) or ECG (16) criteria. Group 1 comprised 30 patients, who developed no or only a small infarct peri-operatively; group 2 had eleven patients with large infarcts. The patients of group 1 had a greater exercise tolerance pre-operatively than those of group 2 (118 +/- 28 vs 91 +/- 45 Watt; P less than 0.05) and had a higher revascularization time (interval between end of the cardiac catheterization and onset of reperfusion: 154 +/- 58 vs 264 +/- 173 min; P less than 0.05). Collaterals to the area supplied by the treated vessel were observed only in group 1 patients (10), but not of those in group 2. Age, history of previous infarction, number of diseased coronary arteries, ischaemic reaction during ergometry, initial left-ventricular ejection fraction and symptoms after onset of complications were without significance. It is concluded that an aortocoronary bypass operation should be performed as early as possible after post-TCA vascular complication in order to prevent the development of larger infarcts.

Angioplasty, Balloon↗
PubMed1986

In situ quantification of T-cell subsets, NK-like cells and macrophages in Hodgkin's disease: quantity and quality of infiltration density depends on histopathological subtypes.

The present investigation addressed itself to the in situ quantification of reactive cells in tumour tissues affected by Hodgkin's disease. Immunostaining was used for identification and stereology was used for enumeration of T-helper/inducer (CD4+) T-cytotoxic/suppressor (CD8+), NK-like (Leu7+) and cells of macrophage origin (Mono 2+). The evaluation of 50 cases showed that CD4+ cells always outnumbered CD8+ cells but the degree of this predominance varied depending on the histopathological subtypes (n.s. greater than m.c. greater than l.d.). Lymph nodes contained more CD4+ as well as CD8+ cells compared to spleens. Therefore, no changes in the T4:T8 ratio occurred. No significant differences in the densities of NK-like cells were observed, comparing the different histopathological subtypes as well as lymph nodes and spleens. Similarly, macrophage (M phi) density was comparable in all histopathological subtypes. However, lymph nodes contained significantly more M phi compared to spleens. On comparison of reactive cells in Hodgkin's tissues to non-Hodgkin lymphomas (79 cases) and normal controls (7 cases) significantly higher numbers of CD4+, CD8+ and Mono 2+ cells were found in Hodgkin's compared to non-Hodgkin's lymphomas. In contrast, the density of NK-like cells in NHL as well as in normal tissues was fivefold compared to that observed in Hodgkin's tissues.

Hodgkin Disease↗
PubMed1986

[Value of surgery in the treatment of small-cell bronchial cancer].

As a result of strict cooperation between oncologists, surgeons and radiotherapists we developed a comprehensive combination therapy for small cell lung cancer (limited disease): Stage I, II: radical tumour resection; chemotherapy (Cohen); cerebral radiation prophylaxis; locoregional radiotherapy; Stage III: Chemotherapy; cerebral radiation prophylaxis; lung resection in an extension required by the initial tumour; radiotherapy (local). The preliminary results in 15 patients are encouraging.

Adult↗
PubMed1986

Macrophage infiltration in non-Hodgkin's lymphomas: a quantitative in situ study.

The frequency and distribution pattern of macrophages within 93 non-Hodgkin's lymphomas (NHL) were evaluated in situ by immunomorphometry using stereological methods. For the identification of macrophages (M phi), several antibodies (Mono 1, Mono 2, OKM 1) reactive with surface antigens on cells of the monocyte-macrophage series and cytochemical staining for acid phosphatase were applied. The average number of macrophages within lymph node tissue of NHL was 6,299 +/- 760 cells/microliter (similar to reactive lymphatic tissue: 6,559 +/- 1,027). The highest number of infiltrating macrophages was detected in immunoblastic NHL (17,306 +/- 2,773), differing significantly from other histological subtypes and reactive lymphatic tissue (p less than 0.005). The possible impact of tumor-infiltrating macrophages on lymphoma cell proliferation and differentiation is discussed.

Antibodies, Monoclonal↗
PubMed1986

The role of surgery in the combined management of small cell bronchial carcinoma.

Even after the introduction of modern chemotherapeutic regimens and radiotherapeutic approaches in the treatment of small cell bronchial carcinoma (SCLC), the results are still disillusioning. Long-term remissions are rare even in patients with limited disease. We review 66 patients with SCLC (limited disease) which we treated either by single therapies (chemotherapy, radiotherapy, surgery n = 16), or by different combinations of the possible therapeutic measures. Favorable results were achieved by a comprehensive treatment including operation, chemotherapy and prophylactic cranial and local irradiation: 11 of 15 patients survived 3 to 97 months after the onset of therapy, 4 of those more than 24 months. Based on our own experience and the results of other authors we feel that surgical resection should be again included as an essential part of treatment: tumor resection is the rational primary therapeutic approach for cases without lymph node metastases in the mediastinum and for tumors of uncertain histological type. If there is evidence of lymph node involvement in the mediastinum (N2), surgery should be performed after a remission has been successfully induced by chemotherapy (so-called adjuvant surgery).

Adult↗
PubMed1986

Growth fraction of tumour cells and infiltration density with natural killer-like (HNK1+) cells in non-Hodgkin lymphomas.

Two markers for cells in the growth fraction, the T9 antigen (i.e. the transferrin receptor) and the T10 antigen were investigated in frozen tissue sections of 105 non-Hodgkin lymphomas (NHL). The results were correlated with the histological subtype and the pattern of tumour infiltration by reactive cells. Special attention was directed to the density of natural killer (NK)-like cells using the anti-HNK1 (Leu7) antibody since the transferrin receptor (tfr) or other growth-associated membrane structures may serve as target for NK cells. Our study confirms a relationship between number of tumour cells with the T9 marker and tissue infiltration by HNK1+ cells in NHL of low (chronic lymphocytic leukaemia, hairy-cell leukaemia, immunocytic lymphoma, centroblastic-centrocytic lymphoma) and intermediate (centrocytic and centroblastic lymphoma) but not in NHL of high malignant grade (immunoblastic and lymphoblastic lymphoma). Comparable results were obtained with the T10 antigen although the correlation was less close. The percentage of cells in the growth fraction, defined by the expression of the T9 and T10 marker, corresponded with prognostically unfavourable subgroups with the remarkable exception of follicular NHL of centroblastic-centrocytic type. This lymphoma showed high numbers of cells with the T9 and the T10 marker in a microenvironment resembling normal germinal centres in many aspects.

Antigens, Surface↗
PubMed1986

[Studies of immunologic markers in malignant lymphomas: diagnostic and prognostic relevance].

Immunological markers important for the diagnosis of the following subtypes of Non-Hodgkin lymphomas (NHL) are discussed: lymphocytic lymphomas (i.e.: B-CLL, immunocytic NHL, prolymphocytic- and hairy cell leukemia), NHL derived from follicular center cells (centrocytic, centroblastic/centrocytic and centroblastic NHL), lymphomas with blastoid appearance (various forms of lymphoblastic and "large cell" NHL) and disorders of T lymphocytes (NHL) of peripheral T cells and T gamma lymphocytosis with neutropenia). In these disorders, the patterns of leukocyte surface antigens like T1 (CD5), Tac (CD25), CALLA (CD10), FMC7, Tü1 and Tü33 as well as staining for the terminal transferase (TdT) and of dendritic follicular cells have been examined by investigating specimens of a large group of patients. Apart from the diagnostical value, these evaluations have contributed to a better understanding of normal B cell differentiation whereas the role of marker studies as independent prognostic factors requires additional prognostic studies. Finally, the value of immunoperoxidase or alkaline phosphatase staining of cryostat sections of bone marrow biopsies is emphasized, which allowed the diagnosis and classification of 74% of 92 NHL studied by this technique.

Antibodies, Monoclonal↗
PubMed1986

Receptors for the third component of complement: their association with maturation stage in non-Hodgkin lymphomas (NHL) and their possible implication with the development of follicular structures.

In the majority of 188 non-Hodgkin lymphomas (NHL) investigated in this study, we found a simultaneous expression of the receptors for c3b and c3d (CR1 & CR2; cccorr = 0.69, P less than 0.0005). An analysis of the different histological entities of the Kiel classification revealed that this coexpression was most pronounced for germinal centre-derived (cccorr = 0.63, P = 0.0004) and immunocytic NHL (cccorr = 0.86, P = 0.0024), whereas in chronic lymphocytic leukaemias there tended to be a more heterogeneous pattern of complement receptor (CR) expression (cccorr = 0.29, P greater than 0.10). In contrast to these NHL of mid B cell stage, most of the NHL of early (i.e. acute lymphocytic leukaemia, lymphoblastic NHL) and late B cell stage (i.e. hairy cell leukaemia, immunoblastic NHL, multiple myeloma) did not express either of these receptors. CR positive NHL often showed a follicular arrangement of the neoplastic cells and had higher numbers of T helper/inducer (T4) lymphocytes (PCR1 less than 0.00005, PCR2 less than 0.05). Some cases of mid B cell NHL and all cases of hairy cell leukaemia reacted with antibodies against CR3 (i.e. the ic3b receptor).

B-Lymphocytes↗
PubMed1985

[Infiltration pattern of immunocompetent lymphocytes in the tissue of malignant non-Hodgkin's lymphoma: relationship to histological and clinical parameters].

The number and distribution of reactive cells in more than 100 non-Hodgkin lymphomas (NHL) were evaluated in situ in cryostat sections. The results were compared with histological and clinical findings. 40% of the T cell content of normal lymphatic tissues was found in tissues of B-cell NHL. This corresponds to a mean of 2 X 10(4) reactive T-cells/microliter tumour tissue. The numerical density of natural killer (NK) cells within tumours was similar to that of normal lymphatic tissues (0.5 X 10(4) cells/microliter). The distribution of the reactive cells within the tumours was diffuse except in the case of centroblastic/centrocytic lymphomas. On evaluation of the different histological entities a significant correlation was obtained between number of helper/inducer (TH) cells, TH:T-suppressor (Ts) ratio and prognostically favourable histological subgroups. Furthermore, independent of histological criteria, a close correlation was found between a high number of TH-cells, a high TH:Ts ratio and a favourable clinical course (p less than 0.04). NK-cell infiltration was present to a markedly higher extent in tissues of patients with generalized disease as compared with localized disease (p less than 0.03). Low-grade malignant NHL contained significantly more NK cells than high-grade malignant NHL, as was the case also in treated, as opposed to untreated patients. These findings together suggest that reactive cells influence tumour growth via local interactions. However, tissue distribution and infiltration density of T-cell subpopulations and NK cells were clearly different. Thus, in tumour tissues different and independent immunoregulatory mechanisms seem to be associated with these two lymphocyte subsets.

Antibodies, Monoclonal↗
PubMed1985

[Host reaction in non-Hodgkin's lymphomas: tumor infiltration with monocytes/macrophages].

The number and distribution of macrophages in deep frozen sections of various non-Hodgkin lymphomas (NHL) were evaluated in situ by immunomorphometry using stereological methods. The density of infiltration was correlated with clinical parameters. Several monoclonal antibodies reactive with antigens present on cells of the monocyte-macrophage series were tested in parallel. The density of macrophages within NHL of high malignancy with 11.3 X 10(3) +/- 1739 positive cells/microliter tumour tissue was higher than in those of low malignancy (4.7 X 10(3) +/- 628). The highest value of positive cells was found in immunoblastic lymphoma (17.3 X 10(3) +/- 2773), differing significantly from other histological subtypes of NHL and normal tissue (normal tissue: 6.5 X 10(3) +/- 1027, p less than 0.005). With regard to clinical parameters, localized NHL displayed lower infiltration numbers than generalized NHL. Possible mechanisms of influencing tumour growth are discussed in view of the large infiltration density of the monocyte macrophage series in NHL of unfavourable histology and advanced clinical stage.

Antibodies, Monoclonal↗
PubMed1985

Discrimination of neuroleptics by means of their interaction with amfonelic acid: an attempt to characterize the test.

The non-amphetamine stimulant amfonelic acid (AFA) is known to enhance the effects of haloperidol, trifluoperazine and spiperone but not those of the atypical neuroleptics clozapine, thioridazine, and sulpiride, on the striatal levels of 3,4-dihydroxyphenylacetic acid. Consequently, the interaction between neuroleptics and AFA has been proposed as a test to discriminate typical and atypical neuroleptics. In this study, these findings were confirmed. Essentially the same results were obtained when the levels of homovanillic acid were measured. However, striatal dopamine levels were decreased similarly by combinations of AFA with typical and atypical neuroleptics. A comparison of the results with 17 neuroleptics with their reported clinical liability to cause extrapyramidal symptoms supported the idea that the test may discriminate drugs with a better ratio of therapeutic vs side effects. A series of antidepressants and alpha-noradrenergic antagonists possessing antidopaminergic properties was found to perform like atypical neuroleptics, i.e. their effects on dopamine metabolites were not enhanced by AFA.

3,4-Dihydroxyphenylacetic Acid↗
PubMed1985

CFU-gm assay, cytochemical and electron microscopic studies in agar in patients with preleukemic syndrome and aplastic anemia.

Thirty-seven patients with chronic cytopenia were studied using a CFU-gm assay in agar. Cell proliferation was evaluated on days 2, 3, 5, 7, and 10 of incubation. Growth patterns were different in cultures of hematologically healthy persons versus patients with preleukemic syndrome (PL) and aplastic anemia (AA). Three types of PL syndrome and two types of AA (C1 and C2) were distinguished. Bone marrow dysfunction was evaluated further using cytochemistry and electron microscopy to morphologically study cell proliferation in vitro. Cytochemical staining performed in agar demonstrated well-defined maturation defects in myelopoietic precursor cells from the bone marrow of PL patients. Electron microscopic findings of Auer-body-like inclusions in "statu nascendi" in the vacuoles of preleukemic cells supported our results. PL patient groups at high risk for development of overt leukemia and patients with grave prognosis in AA were distinguished. Our results are relevant for the clinical diagnosis and prognosis of patients with cytopenia.

Aged↗
PubMed1985

Infiltration density of HNK 1 positive cells in non-Hodgkin's lymphomas depends on histologic subtype: an in situ morphometric analysis.

Numbers and distribution of HNK-1 (Leu 7) positive cells within 115 malignant Non-Hodgkin's lymphomas (NHL) were evaluated in situ by immunomorphometry. Results on the infiltration were related to histological and clinical parameters. A mean of 4.099 +/- 350 HNK 1+ cells/microliter tumor tissue was found, which was comparable to normal (reactive) lymphatic tissues (4.441 +/- 1.235) and was about a quarter of the population density of T helper/inducer (TH) and T cytotoxic/suppressor (TS) lymphocytes together. The distribution of HNK 1+ cells within the tumors was diffuse except in nodular lymphomas of follicular center cell origin (centroblastic/centrocytic = cb/cc NHL). When evaluating the different histological subgroups, the highest number of HNK 1+ cells was found within the tumor areas of cb/cc, which contained about three times as many positive cells as the other NHL. High numbers were also found in the diffuse variant of cb/cc but not in centrocytic NHL. Different degrees of HNK 1+ cell densities were observed in lymphocytic lymphomas (4.426 +/- 754), with high numbers in about 30% of the patients. Splenic tissues of 6 hairy cell leukemias displayed lower numbers of HNK 1+ cells as compared with other low grade malignant NHL. In "large cell" NHL, the lymphoblastic subtype showed only sparse HNK 1+ cells (1.345 +/- 386). The number was significantly reduced in comparison to all other NHL (p less than 0.01) and markedly lower than in the other NHL of high malignancy (immunoblastic and centroblastic NHL, p less than 0.02). The diminuation was not due to a simple dilution phenomenon in a rapidly proliferating tumor, as TH and TS infiltrates were comparable to other NHL. Correlating results with the clinical course (available in 58 patients), significantly higher numbers of HNK 1+ cells were found in NHL of low malignancy (p less than 0.02), but patients with a favourable course did not differ from those with progressive disease (p less than 0.5). Patients treated by cytotoxic drugs showed higher numbers of HNK 1+ cells than those before or without treatment (p less than 0.02). Results on TH and TS cell numbers in comparison to HNK 1+ cells showed completely different patterns of infiltration.

Antibodies, Monoclonal↗
PubMed1985

Treatment with low dose human recombinant interferon-alpha-2-ARG induces complete remission in patients with hairy cell leukemia.

Five cases with advanced hairy cell leukemia refractory to treatment with splenectomy and chemotherapy as well as one patient presenting with a stage-A response to splenectomy were treated with rhu-IFN-alpha 2-arg. 5 X 10(6) were administered intramuscularly every day. Both patients, with advanced disease resistant to conventional therapy and treated for six or more months with rhu-IFN-alpha 2-arg, achieved complete clinical remissions. Three further cases treated for less than half a year and also with advanced disease achieved partial remission states with marked reduction of circulating hairy cells and with recovery of normal hemopoiesis. Minimal residual disease in the remaining patient during a three-month period of treatment did not respond. Side effects of rhu-IFN-alpha 2 low dose therapy were minimal in 5 cases and comprised a severe leukopenia reversible after dose reduction in one patient.

Adult↗
PubMed1985

[Immunocytologic diagnosis of leukemia and lymphoma: monoclonal antibodies in the differential diagnosis of hematologic neoplasms].

Malignant lymphomas and leukemias are related to maturation stages of distinct subpopulations of hematopoietic or lymphoid cells as defined by immunocytological methods. We first describe various monoclonal antibodies, and the methodology employed in our investigations and report on recent developments in the standardization of these reagents (part 1). The maturation sequence of normal lymphoid cells and their precursors in the bone marrow, thymus and the peripheral lymphoid organs are discussed in part 2. Typical immunomorphological findings in Non Hodgkin Lymphomas (NHL) are summarized in respect to lymphocytic NHL (CLL, lymphoplasmocytoid NHL, hairy cell and prolymphocytic leukemia, some lymphomas of peripheral T-lymphocytes), to NHL of follicular center cells (centroblastic-centrocytic, centrocytic NHL), to "large cell" NHL (centroblastic, immunoblastic NHL, large cell NHL derived from T-lymphocytes or "lymphomas" of macrophage origin) and to lymphoblastic NHL (derived from T-lymphocytes, pre-B lymphocytes and Burkitt-type). Findings in multiple myeloma are also summarized in part 3. Immunocytological features of the normal Myelo-, Mono-, Erythro- and Thrombopoieses are discussed in part 4. The reactivity of some monoclonal antibodies with precursor cells of these cells (CFU-GM, BFU-e and CFU-e, CFU-M) are also described. Finally we summarized the immune phenotype of acute leukemias (part 5) in respect to acute lymphoid leukemias (cALL, T-ALL, O-ALL, B-ALL), to acute non lymphoid leukemias (M1-M6 type according to the FAB-Classification) and to blastic stages of chronic leukemias.

Antibodies, Monoclonal↗